Competitive immunoassay for simultaneous detection of imidacloprid and thiacloprid by upconversion nanoparticles and magnetic nanoparticles

2019 ◽  
Vol 26 (23) ◽  
pp. 23471-23479 ◽  
Author(s):  
Zhexuan Tao ◽  
Jiaqi Deng ◽  
Yan Wang ◽  
He Chen ◽  
Yuan Ding ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Simultaneous Detection ◽  
Upconversion Nanoparticles ◽  
Competitive Immunoassay

Multiplex bead-array competitive immunoassay for simultaneous detection of three pesticides in vegetables

2013 ◽  
Vol 180 (5-6) ◽  
pp. 387-395 ◽  
Author(s):  
Yirong Guo ◽  
Jie Tian ◽  
Chizhou Liang ◽  
Guonian Zhu ◽  
Wenjun Gui
Keyword(s):  
Simultaneous Detection ◽  
Competitive Immunoassay ◽  
Multiplex Bead

Simultaneous detection of multiple viruses based on chemiluminescence and magnetic separation

2017 ◽  
Vol 5 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Zeeshan Ali ◽  
Jiuhai Wang ◽  
Yongjun Tang ◽  
Bin Liu ◽  
Nongyue He ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Throughput ◽  
Dna Hybridization ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Reliable Detection ◽  
Automated Platform ◽  
Modified Magnetic Nanoparticles ◽  
Surface Modified ◽  
Multiple Pathogens

In this report, a DNA hybridization based chemiluminescent detection method has been proposed for reliable detection of multiple pathogens. The use of surface modified magnetic nanoparticles can help to integrate this system into an automated platform for high throughput applications.

scholarly journals SIMULTANEOUS ELECTROCHEMICAL DETECTION OF ASCORBIC ACID, DOPAMINE, AND URIC ACID AT MAGNETIC NANOPARTICLES/REDUCED GRAPHENE OXIDE MODIFIED ELECTRODE

2021 ◽  
Vol 83 (3) ◽  
pp. 85-92
Author(s):  
Azleen Rashidah Mohd Rosli ◽  
Farhanini Yusoff ◽  
Saw Hong Loh ◽  
Hanis Mohd Yusoff ◽  
Muhammad Mahadi Abdul Jamil ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Magnetic Nanoparticles ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Differential Pulse ◽  
Reduced Graphene

A magnetic nanoparticles/reduced graphene oxide modified glassy carbon electrode (MNP/rGO/GCE) was fabricated via one-step facile synthesis route for the simultaneous determination of ascorbic acid (AA), dopamine (DA), along with uric acid (UA). A series of diseases and disorders has been associated with irregular levels of these respective analytes, thus early detection is highly crucial. Physical and electrochemical characterization of the modified electrode was conducted by using Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) analysis, X-Ray Diffraction (XRD) analysis and Brauneur-Emmet-Teller (BET), Cyclic Voltammetry (CV) and Electron Impedance Spectroscopy (EIS). The results obtained confirmed the formation of MNP/rGO composite. Differential pulse voltammetry (DPV) of MNP/rGO/GCE displays three well-defined peaks which associated to AA, DA and UA, respectively. The response towards DA is linear in the concentration range of 15 nM to 100 µM with a detection limit of 0.19 nM while a response to AA and UA is also linear in the concentration range of 10 µM to 100 µM with a limit of detection 0.22 µM and 45 nM respectively. The proposed modified electrode offers a good response towards simultaneous detection of three different electroactive species with excellent electron transfer rate, great capacitance and ideal diffusive control behavior.

Simultaneous Detection of Seven Drugs of Abuse by the TriageTM Panel for Drugs of Abuse

1992 ◽  
Vol 38 (9) ◽  
pp. 1678-1684 ◽  
Author(s):  
K F Buechler ◽  
S Moi ◽  
B Noar ◽  
D McGrath ◽  
J Villela ◽  
...  
Keyword(s):  
Urine Sample ◽  
Drug Class ◽  
Drugs Of Abuse ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Threshold Concentration ◽  
Competitive Immunoassay ◽  
Immobilized Antibodies ◽  
Procedural Control ◽  
To Come

Abstract This novel, competitive immunoassay simultaneously detects seven drugs of abuse in urine. A urine sample is placed in contact with lyophilized reagents, the reaction mixture is allowed to come to equilibrium (10 min), and then the whole mixture is applied to a solid phase that contains various immobilized antibodies in discrete drug-class-specific zones. After a washing step, the operator visually examines each zone for the presence of a red bar. The method incorporates present threshold concentrations that are independent for each drug. In the absence of drug or in the presence of drug in quantities less than the threshold concentration, no colored bar is visible. Samples containing drug(s) at or above the threshold concentration cause a red bar to appear for the appropriate drug(s). Positive and negative procedural control zones are incorporated into each determination. The performance of the assay methodology matches that of instrumented immunoassay systems.

scholarly journals Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay

2020 ◽  
Vol 14 (1) ◽  
pp. 59-69
Author(s):  
Milka Atanasova ◽  
Yavor Ivanov ◽  
Elena Zvereva ◽  
Anatoly Zherdev ◽  
Tzonka Godjevargova
Keyword(s):  
Magnetic Nanoparticles ◽  
Simultaneous Determination ◽  
Magnetic Nanoparticle ◽  
Limit Of Detection ◽  
Animal Health ◽  
Simultaneous Detection ◽  
Penicillin G ◽  
Frequent Use ◽  
Simultaneous Immunoassay

Background: Antibiotic residues are a problem of increasing importance and have direct consequences for human and animal health. The frequent use of antibiotics in veterinary practice causes their excretion in milk in dairy cattle. This way, they can easily enter the human body through the consumption of milk and dairy products. Objectives: This induces the need for accurate and sensitive methods to monitor antibiotic levels in milk. The aim of this study was to develop a rapid and sensitive magnetic nanoparticle-based fluorescence immunoassay for the simultaneous detection of chloramphenicol and penicillin G in milk. Methods: Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Chloramphenicol-Ovalbumin and Chloramphenicol-Ovalbumin-Fluorescein-5-isothiocyanate conjugates were prepared. Penicillin G – ATTO 633 fluorescent conjugate was synthesized. Antibodies against chloramphenicol and penicillin G were immobilized onto the magnetic nanoparticles. The competitive fluorescent immunoassay was developed. The optimal concentration of the antibody-magnetic nanoparticles and the fluorescent conjugates for the assay was determined. The calibration curves for the antibiotics in buffer and milk were plotted. Fluorescent immunoassay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. Results: The limit of detection by the simultaneous immunoassay of chloramphenicol and penicillin G in milk was 0.85 ng/mL and 1.6 ng/mL, respectively. The recovery of different concentrations of chloramphenicol and penicillin G in milk samples varied from 98% to 106%. Conclusions: A rapid and sensitive magnetic nanoparticle-based immunofluorescent assay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. The magnetic nanoparticles ensured rapid and easy procedure.

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