Adulticidal activities of Cymbopogon citratus (Stapf.) and Eucalyptus globulus (Labill.) essential oils and of their synergistic combinations against Aedes aegypti (L.), Aedes albopictus (Skuse), and Musca domestica (L.)

2020 ◽  
Vol 27 (16) ◽  
pp. 20201-20214 ◽  
Author(s):  
Mayura Soonwera ◽  
Sirawut Sittichok
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jirapon Aungtikun ◽  
Mayura Soonwera

AbstractImproved natural adulticidal agents against mosquito vectors are in urgent need, and essential oils from Cinnamomum plants can assume this role quite readily. Cinnamomum verum, C. cassia, and C. loureiroi essential oils (EOs) were extracted from the barks and evaluated for their chemical composition by GC–MS. The major constituent of the three EOs was cinnamaldehyde. WHO susceptibility tests on individual and combined EOs as well as cinnamaldehyde were conducted against female adults of Aedes aegypti and Aedes albopictus. All EO combinations exhibited a synergistic effect, manifesting a higher toxicity, with a synergistic value ranging from 2.9 to 6.7. Their increasing mortality value was improved between 16.0 to 41.7%. The highest synergistic effect was achieved by an EO combination of 0.5% C. cassia + 0.5% C. loureiroi, while the highest insecticidal activity was achieved by 2.5% C. verum + 2.5% C. cassia and 1% cinnamaldehyde, with a knockdown and mortality rate of 100% and a KT50 between 0.7 and 2.1 min. This combination was more toxic to both mosquito species than 1% w/v cypermethrin. These findings demonstrate that cinnamaldehyde and synergistic combinations of C. verum + C. cassia EOs and C. cassia + C. loureiroi EOs have a high insecticidal efficacy against Aedes populations.


The Biologist ◽  
2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Maureen Leyva ◽  
María Del Carmen Marquetti ◽  
Domingo Montada ◽  
Juan Payroll ◽  
Ramón Scull ◽  
...  

El extensivo uso de los insecticidas ha provocado daños medioambientales de diversos tipos y generado resistencia en el sitio diana de control de algunos vectores. El estudio de la actividad insecticida de plantas, constituye un objetivo primordial en la búsqueda  de fuente de compuestos bioplaguicidas para la preparación de formulados. El objetivo propuesto para este trabajo consistió en; evaluar la actividad bioplaguicida de los aceites esenciales de <em>Eucalyptus globulus </em>(Labill, 1800<em>) </em>y<em>  Bursera graveolens </em>((Kunth) Triana &amp; Planch, 1872)<em> </em>sobre <em>Aedes aegypti</em> (Linnaeus, 1762),<em> Aedes albopictus </em>(Skuse<em>, </em>1894) <em>y Culex quinquefasciatus</em><em> </em>(Say, 1823)<em>. </em>Se evaluó la actividad larvicida siguiendo la metodología estandarizada de la Organización Mundial de la Salud (OMS) y en el caso de la  actividad adulticida se utilizó la metodología de impregnación de papeles según OMS y la impregnación de botellas según lo establecido por el Centro para el Control de Enfermedades (CDC). Los aceites evaluados evidenciaron una significativa actividad larvicida en las poblaciones de mosquitos estudiadas con valores de concentraciones letales medias calculadas inferiores a 100 mg·L<sup>-1</sup>. Las concentraciones estudiadas sobre la fase adulta que  favorecieron el 100 % de derribo de los individuos expuestos a los 30 min oscilaron entre 10 y 60 mg·mL<sup>-1</sup>. Los resultados obtenidos con<em> B. graveolens</em> son los primeros reportes de esta actividad, para la región de las Américas. Estos  resultados evidenciaron que los aceites esenciales de estos recursos forestales pueden ser considerados candidatos promisorios para el control de mosquitos de importancia médica.


Insects ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 128 ◽  
Author(s):  
Ho Dung Manh ◽  
Do Thi Hue ◽  
Nguyen Thi Thanh Hieu ◽  
Doan Thi Thanh Tuyen ◽  
Ong Thi Tuyet

The larvicidal activity of essential oils (EOs) extracted from Cymbopogon citratus, Cymbopogon winterianus, Eucalyptus citriodora, and Eucalyptus camaldulensis aromatic plants grown in Vietnam was evaluated on Aedes aegypti larvae. The EOs were hydro-distilled in a Clevenger-type apparatus. The EOs were analyzed by gas chromatography–mass spectrometry (GC–MS). The mortality rates obtained from the bioassays were used to calculate the lethal concentrations (LC50) of the EOs by the probit analysis method. These essential oils exhibited toxicity to the larvae of Aedes aegypti. Results were obtained for Cymbopogon citratus (LC50 = 120.6 ppm), Cymbopogon winterianus (LC50 = 38.8 ppm), Eucalyptus citriodora (LC50 = 104.4 ppm), and Eucalyptus camaldulensis (LC50 = 33.7 ppm). The essential oils of Eucalyptus camaldulensis and Cymbopogon winterianus were found to be the most efficient, and their respective values of LC50 were 33.7 ppm, 38.8 ppm. In conclusion, this research adds to the growing body of literature on natural larvicides from essential oils against Aedes aegypti mosquitoes.


2009 ◽  
Vol 57 (23) ◽  
pp. 11127-11133 ◽  
Author(s):  
Hui-Jing Gu ◽  
Sen-Sung Cheng ◽  
Chun-Ya Lin ◽  
Chin-Gi Huang ◽  
Wei-June Chen ◽  
...  

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Sonnia J. Giroth ◽  
Janno B. B. Bernadus ◽  
Angle M. H. Sorisi

Abstract: Aedes aegypti and Aedes albopictus are known as the two main vectors of DHF disease. One way to control DHF is by breaking the chain of spread in the larval phase with larvicides. The use of organic larvicides derived from plants is in great demand, one of which is extracts of lemongrass (Cymbopogon citratus). This study aims to determine the level of efficacy or the effect of concentration of lemongrass extract solution on mortality rates of Aedes sp. larvae. This is an experimental study using 40 instar larvae III / IV of Aedes sp. which were given the intervention of lemongrass plant extracts with a concentration of 5%, 10%, 15%, and 20%. This experiment was carried out twice. Four observations were made every 6 hours. Probit analysis was performed to determine Lethal Concentration (LC50 and LC90) and Lethal Time (LT50 and LT90). The analysis showed that the lemongrass plant extract at a concentration of 20% had a significant difference with the concentration of 5%, 10%, 15%, and the control group (p <0.05). In conclusion, extract of lemongrass (Cymbopogon citratus) with a concentration of 20% is effective for killing larvae of Aedes sp.Keywords: Aedes sp., Cymbopogon citratus, larval mortality  Abstrak: Nyamuk Aedes aegypti dan Aedes albopictus diketahui sebagai dua vektor utama dari penyakit DBD. Salah satu cara pengendalian DBD adalah dengan memutus rantai penyebaran pada fase larva dengan larvasida. Pemanfaatan larvasida organik atau alami yang berasal dari tumbuh-tumbuhan banyak diminati, salah satunya adalah ekstrak tanaman serai (Cymbopogon citratus). Penelitian ini bertujuan mengetahui tingkat keampuhan atau pengaruh konsentrasi larutan ekstrak tanaman serai terhadap tingkat mortalitas larva nyamuk Aedessp. Penelitian ini bersifat eksperimental sederhana menggunakan 40 ekor larva instar III/IV nyamuk Aedes sp. yang diberikan intervensi ekstrak tanaman serai dengan konsentrasi 5%, 10%, 15%, dan 20%. Pengamatan dilakukan setiap 6 jam sebanyak 4 kali, dengan 2 kali percobaan. Analisis probit dilakukan untuk mengetahui Lethal Concentration (LC50 dan LC90) dan Lethal Time (LT50dan LT90). Hasil analisis menunjukkan bahwa ekstrak tanaman serai pada konsentrasi 20% memiliki perbedaan signifikan dengan konsentrasi 5%, 10%, 15%, dan kelompok kontrol (p < 0,05). Simpulan penelitian ialah ekstrak tanaman serai dengan konsentrasi 20% efektif untuk mematikan larva Aedes sp.Kata Kunci: Aedes sp., Cymbopogon citratus, mortalitas larva


Author(s):  
Puput Mirawati ◽  
Eva Susanty Simaremare ◽  
Rani Dewi Pratiwi

Zodia (Evodia suaveolens Scheff) and lemongrass (Cymbopogon citratus) are native Indonesian plants with repellant activity against mosquitoes and insects. The purpose of this research were to formulate and evaluate the repellant activity of zodia-serai lotion against Aedes aegypti and determine the protection power of three formulas of lotion. The method used in this research including steam distillation of essential oil from zodia leaves and lemongrass, formulation and evaluation of lotions, power protection test, and data analysis. The result of this research showed that lotion had acidic pH of 5, and they were stable without separation after stability evaluation with centrifugation. The most effective repellant activity of the lotion was exhibited at 0 hours with 100% of repellency. The best combination of essential oils of zodia (Evodia suaveolens Scheff) and lemongrass (Cymbopogon citratus) as Aedes aegypti repellent was at ratio of 7:3.


2021 ◽  
Author(s):  
Mayura Soonwera ◽  
Tanapoom Moungthipmalai ◽  
Wacharaporn Takawirapat ◽  
Sirawut Sittichok

Abstract Natural ovicidal and repellent agents against Periplaneta americana are in urgent need, and plant essential oils (EOs) can assume this role quite readily. In this study, ovicidal and repellent activities against Periplaneta americana of EOs from Cymbopogon citratus, Cinnamomum verum, Eucalyptus globulus, Illicium verum, and Zanthoxylum limonella in soybean oil and in ethyl alcohol were determined by topical and dual-choice assays, as well as 10% cypermethrin and a combined formulation of 5% C. verum EO + 5% I. verum EO. Cypermethrin at 10% provided the highest toxicity (100% inhibition rate) against the eggs, but only slightly higher than that (99.3%) provided by the combined EO formulation, while the highest repellent activity against the adults was provided by the combined formulation (89.5% repelled cockroaches at 48 h after treatment). In addition, all EO formulations in soybean oil provided higher ovicidal and repellent activities than in ethyl alcohol. To conclude, the combined EO formulation in soybean oil can replace cypermethrin because their efficacy was nearly equivalent, but the combination should be much safer to use.


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