scholarly journals Uji Efikasi Ekstrak Tanaman Serai (Cymbopogon citratus) terhadap Tingkat Mortalitas Larva Nyamuk Aedes sp.

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Sonnia J. Giroth ◽  
Janno B. B. Bernadus ◽  
Angle M. H. Sorisi
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Lethal Concentration ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Larval Phase ◽  
Significant Difference ◽  
Lethal Time

Abstract: Aedes aegypti and Aedes albopictus are known as the two main vectors of DHF disease. One way to control DHF is by breaking the chain of spread in the larval phase with larvicides. The use of organic larvicides derived from plants is in great demand, one of which is extracts of lemongrass (Cymbopogon citratus). This study aims to determine the level of efficacy or the effect of concentration of lemongrass extract solution on mortality rates of Aedes sp. larvae. This is an experimental study using 40 instar larvae III / IV of Aedes sp. which were given the intervention of lemongrass plant extracts with a concentration of 5%, 10%, 15%, and 20%. This experiment was carried out twice. Four observations were made every 6 hours. Probit analysis was performed to determine Lethal Concentration (LC50 and LC90) and Lethal Time (LT50 and LT90). The analysis showed that the lemongrass plant extract at a concentration of 20% had a significant difference with the concentration of 5%, 10%, 15%, and the control group (p <0.05). In conclusion, extract of lemongrass (Cymbopogon citratus) with a concentration of 20% is effective for killing larvae of Aedes sp.Keywords: Aedes sp., Cymbopogon citratus, larval mortality  Abstrak: Nyamuk Aedes aegypti dan Aedes albopictus diketahui sebagai dua vektor utama dari penyakit DBD. Salah satu cara pengendalian DBD adalah dengan memutus rantai penyebaran pada fase larva dengan larvasida. Pemanfaatan larvasida organik atau alami yang berasal dari tumbuh-tumbuhan banyak diminati, salah satunya adalah ekstrak tanaman serai (Cymbopogon citratus). Penelitian ini bertujuan mengetahui tingkat keampuhan atau pengaruh konsentrasi larutan ekstrak tanaman serai terhadap tingkat mortalitas larva nyamuk Aedessp. Penelitian ini bersifat eksperimental sederhana menggunakan 40 ekor larva instar III/IV nyamuk Aedes sp. yang diberikan intervensi ekstrak tanaman serai dengan konsentrasi 5%, 10%, 15%, dan 20%. Pengamatan dilakukan setiap 6 jam sebanyak 4 kali, dengan 2 kali percobaan. Analisis probit dilakukan untuk mengetahui Lethal Concentration (LC50 dan LC90) dan Lethal Time (LT50dan LT90). Hasil analisis menunjukkan bahwa ekstrak tanaman serai pada konsentrasi 20% memiliki perbedaan signifikan dengan konsentrasi 5%, 10%, 15%, dan kelompok kontrol (p < 0,05). Simpulan penelitian ialah ekstrak tanaman serai dengan konsentrasi 20% efektif untuk mematikan larva Aedes sp.Kata Kunci: Aedes sp., Cymbopogon citratus, mortalitas larva

scholarly journals Effectiveness Test of Papaya Leaves Extract (Carica papaya L.) as Antihelmintics of Ascaridia galli Worm

2019 ◽  
Vol 15 (2) ◽  
pp. 97
Author(s):  
Farindira Vesti Rahmasari ◽  
Fikri Adhi Wibowo
Keyword(s):  
Carica Papaya ◽  
Ethanol Extract ◽  
Negative Control ◽  
Probit Analysis ◽  
Lethal Concentration ◽  
Control Group ◽  
Ascaridia Galli ◽  
Significant Difference ◽  
Lethal Time ◽  
Lc50 And Lc90

Abstract: Introduction: Soil-transmitted helminth is a group of nematoda worm parasite that causes infection in human because the egg is swallowed or direct contact with the larva. More than two billion people in the world had infection for at least one spesies of the nematoda worm, especially A. lumbricoides, T. trichiura and A. duodenale. Ascaridia galli is a worm parasite that classifeid in nematoda fillum. Ascaridia worm has the same genus with Ascaris Lumbricoides that infect human. Antihelmintic is a drug that can eradicate the worm in human and animal body. There is a side effect in the antihelmintic drug like Mebendazole so another alternative like organical antihelmintic from papaya leaves (Carica papaya L.) is needed. This research is a true experiment with post test control group design. The subjects were 160 Ascaridia galli worm which were divided into 5 worms in each test group (5%, 10%, 20%, 40%, 60%, dan 80%), positive control group (Pirantel pamoat 0,5%) and negative control  repeated as many as 4 replications. The treatment was given for 12 hours and observed the number of dead worm at each hour. Data were analyzed by statistical test of Kruskal Wallis Test and followed by post-hoc Mann-Whitney Test. It is also tested the probit analysis to determine lethal time (LT50 and LT90) and lethal concentration (LC50 and LC90).There was a significant difference (p <0.05) between all test concentrations except between 5% to negative control. It could be evidenced that the papaya leaves (Carica papaya L.) has an antihelmintic effect on Ascaridia gall. The results of probit analysis for LC50 and LC90 were 6,182% and 14,422% respectively. It is known that LT50 at concentrations of 25%, 10%, 20%, 40%, 60%, and 80% respectively were 11,84 hours, 10,536 hours, 9,328 hours, 6,794 hours, 5,472 hours and 2,892 hours. While the LT90 at concentrations of 25%, 10%, 20%, 40%, 60%, and 80% respectively were 13,608 hours, 12,303 hours, 11,095 hours, 8,562 hours, 7,24 hours, dan 4,66 hours.The ethanol extract of Papaya leaves Carica papaya was shown to have an antihelmentic effect on Ascaridia galli effectifically at concentrations of 10%, 20%, 40%, 60% dan 80%. The values of LC50 and LT50 at highest concentration (80%) at the end of the observations are 6,182% and 2,892 hours respectively. The values of LC50 and LT90 at the highest concentration (80%) were 14,442% dan 4,66 hours respectively. The higher concentration of ethanol extract of papaya leaves Carica papaya the greater the effectiveness in killing Ascaridia galli. Keywords: Antihelmintic – Ascaridia galli – Carica papaya L. – Lethal Concentration – Lethal Time

scholarly journals Potensi Ekstrak Jahe Merah (Zingiber Officinale Var. Rubrum) Sebagai Larvasida Alami Aedes Albopictus

2021 ◽  
Vol 8 (2) ◽  
pp. 106
Author(s):  
Qatrinida Qatrinida ◽  
Norfai Norfai ◽  
Kasman Kasman
Keyword(s):  
Aedes Albopictus ◽  
Zingiber Officinale ◽  
Lethal Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Lethal Time

DBD masih merupakan masalah kesehatan masyarakat utama di Indonesia. Upaya pencegahan yang dilakukan saat ini menggunakan insektisida berbahan dasar kimia yang dapat merusak lingkungan juga menimbulkan resistensi. Penggunaan larvasida alami dari tumbuhan dapat menjadi alternatif insektisida, salah satunya adalah jahe merah (Zingiber officinale var. rubrum) yang dapat dijadikan sebagai insektisida alami karena adanya kandungan senyawa aktif. Tujuan penelitian ini adalah untuk mengetahui tingkat kematian, perbedaan rerata kematian larva Aedes albopictus serta mengetahui Lethal Time dan Lethal Concentration. Jenis penelitian ini merupakan penelitian true eksperimental dengan posttest only control group design. Hasil penelitian menunjukkan bahwa kematian larva 100% lebih cepat pada konsenrasi 8% yaitu setelah 6 jam pengujian. Hasil Uji Kruskal Wallis untuk melihat perbedaan rerata kematian larva setiap jam kelompok perlakuan ekstrak jahe merah dengan kelompok kontrol menunjukkan terdapat perbedaan secara signifikan rerata kematian larva (0,000 < 0,05). Hasil uji probit Lethal Time menunjukkan bahwa diperlukan waktu 2,196 jam dalam mematikan 50% larva dan 4,788 jam dalam mematikan 99% larva dengan konsentrasi 8% sedangkan Lethal Concentration menunjukkan bahwa diperlukan konsentrasi sebesar sebesar 0,772% dalam mematikan 50% larva dan 1,973% dalam mematikan 99% larva setelah 9 jam pengukuran. Perlu dilakukan penelitian lebih lanjut untuk mengembangkan ekstrak jahe merah yang aman dan layak pakai namun tidak menghilangkan senyawa yang ada pada ekstrak jahe merah.

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals Toxisitas temephos, minyak atsiri jahe ​(Zingiber officinale Roxb)​, dan ​Bacillus thuringiensis ssp. israelensis​ (Bti) terhadap larva nyamuk ​Ae. aegypti​ dari Sumatra Utara

2018 ◽  
Vol 34 (3) ◽  
pp. 127
Author(s):  
Horas Sihotang ◽  
Sitti Umniyati
Keyword(s):  
Essential Oil ◽  
Larval Mortality ◽  
Zingiber Officinale ◽  
Liquid Solution ◽  
Probit Analysis ◽  
Control Group ◽  
Diagnostic Dose ◽  
Post Test ◽  
Lethal Time ◽  
Lc50 And Lc90

Purpose:  The purpose of this study was to confirm the resistance status and resistance ratio (RR) of  Ae. aegypti larvae to temephos as well as to determine the effectiveness of a liquid solution of Bti H-14 and the essential oil of ginger (Zingiber officinale roxb) against Ae. aegypti larvae from Deli Serdang, North Sumatra.Method: A randomized post-test only control group was con- ducted using 3rd instar larvae of Ae. aegypti as the subjects of this study. Larval mortality was recorded after 24 hours of exposure with diagnostic dose of temephos (0.02ppm) to determine the resistance status. Probit analysis was conducted to determine median lethal time (LT50) and the RR. The concetration of Bti toxicity test was 0.01, 0.013, 0.017, 0.02, 0.03 and 0.04 mL/L and the concentration of toxicity essential oil of ginger test was 66.6, 99.9, 133.2, 166.5, 199.8 and 266.4 ppm.Results: Ae. aegypti larvae were resistant to temephos (0.02ppm) with RR 1.9 < 5. The value of LC50 and LC90 of Bti were 0.014; 0.024 mL/L respectively. The value of LC50 and LC90 of essential oil of ginger solution were 65.6ppm and 129.1ppm respectively.Conclusion: Larvae are resistant to temephos with low resistance criteria. Bti and essential oil of ginger are toxic to Ae. aegypti larvae.

scholarly journals BIOINSECTICIDE AGAINST AEDES AEGYPTI MOSQUITOES DERIVED FROM LEMONGRASS (CYMBOPOGON CITRATUS) LEAVES ETHANOL EXTRACT

2021 ◽  
Vol 20 (1) ◽  
pp. 18-25
Author(s):  
Jennifer Wiranatha ◽  
Robby Makimian ◽  
Rita Dewi
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Ethanol Extract ◽  
Hemorrhagic Fever ◽  
Lethal Effect ◽  
Negative Control ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Chemical Insecticide ◽  
Significant Difference

Introduction: Dengue hemorrhagic fever is one of the most common infectious diseases in Indonesia. The vector of this disease is Aedes aegypti (Ae. aegypti). Dengue hemorrhagic fever can be controlled by a few measures, one of them is using insecticide. However, frequent use of chemical insecticide could lead to resistance and is harmful to non-target organisms. One of the solutions for this problem is the use of bioinsecticide derived from lemongrass (Cymbopogon citratus or C. citratus) leaves extract. Methods: The design of this study is true experimental post-test only control group. The population used are adult Ae. aegypti mosquitoes. For each experiment, 10 mosquitoes are required and given the extract of C. citratus in 2%, 10%, and 20% concentrations, and negative control respectively.The lethal effect of the extract is observed in 10, 30, 60 minutes and 6, 12, 18, 24 hours. Results: There is a significant difference in the lethal effect of Ae. aegypti mosquitoes with variations in duration of exposure to the C. citratus leaves extract (p = 0.007), but no significant difference with variations in extract concentration given (p = 0.281). Conclusion: C. citratus leaves extract has bioinsecticidal effect on Ae. aegypti mosquitoes. The optimal result of mosquito mortality is achieved by using the extract in 20% concentration with duration of 12 hours.

scholarly journals EFFECTIVITY OF EXTRACT LEAF, FRUIT, ROOT MANGROVE Avecennia marina ON Aedes aegypti

2020 ◽  
Vol 2 (3) ◽  
pp. 206-213
Author(s):  
Eti Eriani ◽  
Irwan Effendi ◽  
Dessy Yoswaty
Keyword(s):  
Air Pollution ◽  
Aedes Aegypti ◽  
Experimental Method ◽  
Probit Analysis ◽  
Lethal Concentration ◽  
Mosquito Larvae ◽  
Mangrove Plants ◽  
Mangrove Root ◽  
Lethal Time

Inhibiting the life of the Aedes aegypti mosquito is the mian way that is done to eradicate DBF, which is popularly done by chemically synthetic insecticides can be used air pollution and poisoning of humans so it needs safer natural incecticides such as compounds derived from mangrove plants. Mangrove Avecennia marina contains saponis, flavonoids, and alkaloids can act as stomach poisons resulting in larval death. The study was conducted in Februari-Maret 2019. The purpose of the study was to deteremine the efectiveness of extracts of leaves, fruit Avecennia marina mangrove root that quickly inhibit the growth of Aedes aegypyi larvae. The method used was the experimental method, the location of the research sample taking in Kayu Ara Village, Siak Riau Province. Results showed the mangrove leaves at a concentration of 12% mosquito larvae deaths reached 99 tails in the 120th minute, results showed the mangrove fruit at a concentration of 12% mosquito larvae deaths reached 96 tails in the 120th minute, Results showed the mangrove root at a concentration of 12% mosquito larvae deaths reached 85 tails in the 120th minute, analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. The LC50 probit analysis velue is estimate leaves 2.721, estimate fruit 1.584 dan estimate root 1,232. Whereas at LT50 estimate leaves 2.841, fruit 1.579, root 1.115.

scholarly journals Implementation of a deep learning model for automated classification of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in real time

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song-Quan Ong ◽  
Hamdan Ahmad ◽  
Gomesh Nair ◽  
Pradeep Isawasan ◽  
Abdul Hafiz Ab Majid
Keyword(s):  
Deep Learning ◽  
Aedes Aegypti ◽  
Real Time ◽  
Aedes Albopictus ◽  
Automated Classification ◽  
Expert Performance ◽  
Deep Convolutional Neural Networks ◽  
Significant Difference ◽  
Set Up

AbstractClassification of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) by humans remains challenging. We proposed a highly accessible method to develop a deep learning (DL) model and implement the model for mosquito image classification by using hardware that could regulate the development process. In particular, we constructed a dataset with 4120 images of Aedes mosquitoes that were older than 12 days old and had common morphological features that disappeared, and we illustrated how to set up supervised deep convolutional neural networks (DCNNs) with hyperparameter adjustment. The model application was first conducted by deploying the model externally in real time on three different generations of mosquitoes, and the accuracy was compared with human expert performance. Our results showed that both the learning rate and epochs significantly affected the accuracy, and the best-performing hyperparameters achieved an accuracy of more than 98% at classifying mosquitoes, which showed no significant difference from human-level performance. We demonstrated the feasibility of the method to construct a model with the DCNN when deployed externally on mosquitoes in real time.

scholarly journals Larvicidal Effectiveness of Azolla pinnata against Aedes aegypti (Diptera: Culicidae) with Its Effects on Larval Morphology and Visualization of Behavioural Response

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Nor Shaida Husna Zulkrnin ◽  
Nurul Nadiah Rozhan ◽  
Nur Amanina Zulkfili ◽  
Nik Raihan Nik Yusoff ◽  
Mohd Sukhairi Mat Rasat ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Control Program ◽  
Test Method ◽  
World Health ◽  
Azolla Pinnata ◽  
Agricultural Field ◽  
Aquatic Fern ◽  
Significant Difference

Dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. Aedes aegypti (L.) (Diptera: Culicidae) is a primary vector for dengue viral infections for humans. Current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. The extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and development of insecticide resistance on mosquitoes. Azolla pinnata is an aquatic fern and predominantly used as feed in poultry industry and as fertilizer in agricultural field for enhancing the fertility of rice paddy soil. The present study was conducted to explore the larvicidal efficacy of A. pinnata using fresh and powdered form against late third-stage larvae (6 days, 5 mm in larvae body length) of Ae. aegypti (L.) (Diptera: Culicidae). The larvicidal bioassays were performed using World Health Organization standard larval susceptibility test method for different concentration for powdered and fresh A. pinnata. Powdered A. pinnata concentration used during larvicidal bioassay ranges from 500ppm to 2000ppm; meanwhile, fresh A. pinnata ranges from 500ppm to 9,000,000 ppm. The highest mortality was at 1853 ppm for powdered A. pinnata compared with fresh A. pinnata at 2,521,535 ppm, while the LC50 for both powdered and fresh A. pinnata recorded at 1262 ppm and 1853 ppm, respectively. Finally, the analysis of variance (ANOVA) showed significant difference on Ae. aegypti larval mortality (F=30.439, df=1, p≤0.001) and concentration (F=20.002, df=1, p≤0.001) compared to powdered and fresh A. pinnata at 24-hour bioassay test. In conclusion, the powdered A. pinnata serves as a good larvicidal agent against Ae. aegypti (L.) (Diptera: Culicidae) and this study provided information on the lethal concentration that may have potential for a more eco-friendly Aedes mosquito control program.

scholarly journals The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L.

BIOEDUKASI ◽  
2020 ◽  
pp. 1
Author(s):  
Rima Gloria Purwanto ◽  
Dwi Wahyuni ◽  
Joko Waluyo
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Heating Temperature ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Annona Squamosa ◽  
Forest Region ◽  
Randomized Design ◽  
Significant Difference ◽  
Lower Temperature

Abstract: Aedes aegypti L. is a mosquito carrying dengue virus that causes dengue fever, especially in Southeast Asia which is a tropical rain forest region which is a habitat for mosquito growth. Aedes aegypti L. mosquito control is carried out by chemical means of fogging and using abate but this control actually causes Aedes aegypti L. mosquitoes to become resistant or immune to chemical drugs so that a new breakthrough is created by making bioinsecticides biologically to eradicate the Aedes aegypti L. mosquito. with characteristics that are environmentally friendly and do not kill non-target animals and cause the Aedes aegypti L. mosquito not to become resistant. To be able to realize this desire so that the sugar cane granules extract of Annona squamosa L. containing active compounds in the form of annonain and squamosin so that they are toxic to Aedes aegypti L. mosquito larvae. Further research is to test the heating temperature level of the granules of Annona squamosa L. extract temperature of 40 ° C and 60 ° C which is more deadly of Aedes aegypti L. mosquito larvae. This research method uses a completely randomized design with four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm, each temperature compared with aquadest and abate. Data analyzed using probit analysis to determine the LC50, then followed by a statistical test paired sample T-test with SPSS to find out a significant difference between heating temperatures of 40 ° C to 60 ° C. These results then show that a higher temperature of 60 ° C has a higher level of toxicity compared to a lower temperature of 40 ° C.

scholarly journals UJI EFEKTIVITAS EKSTRAK DAUN CENGKEH (Syzygium aromaticum) SEBAGAI BAHAN DASAR OBAT NYAMUK ELEKTRIK CAIR TERHADAP NYAMUK Aedes aegypti

2014 ◽  
Vol 2 (2) ◽  
pp. 91-96
Author(s):  
Sasono Handito ◽  
Endah Setyaningrum ◽  
Tundjung Tripeni Handayani
Keyword(s):  
Aedes Aegypti ◽  
Lethal Concentration ◽  
Syzygium Aromaticum ◽  
Lethal Time

Aedes aegypti merupakan vektor penyakit Demam Berdarah Dengue (DBD) yang masih menjadi masalah kesehatan di Indonesia. Upaya pengendalian dan pemberantasan Ae. aegypti saat ini banyak dilakukan, salah satunya dengan menggunakan insektisida kimiawi. Penggunaan insektisida kimiawi secara berkelanjutan menimbulkan resistensi pada nyamuk vektor. Telah diketahui bahwa daun tanaman cengkeh (Syzygium aromaticum L) mengandung flavonoid dan saponin yang berpotensi sebagai insektisida. Untuk membuktikan hal tersebut dilakukan penelitian yang bertujuan untuk mengetahui efektifitas dari ekstrak daun cengkeh dengan menuntukan nilai Lethal Concentration (LC50 dan LC90) dan Lethal Time (LT50dan LT90). Penelitian eksperimen yang menggunakan rancangan acak kelompok ini dilakukan dengan lima konsentrasi, yaitu; 10%; 20%; 30%; 40%; dan 50% ekstrak daun cengkeh, dan dengan dua kontrol yaitu, kontrol negatif yang berisi aquades, kontrol positif yang berisi tranflutrin12,38g/l, dengan empat kali pengulangan setiap konsentrasi dan kontrol. Pengamatan terhadap jumlah nyamuk yang mati setiap 5, 10, 20, 40, 60, 120, 240, 480, dan 1440 menit setelah perlakuan. Dari hasil Uji ANOVA yang diuji lanjut dengan Uji BNT diketahui bahwa konsentrasi yang paling efektif dibandingkan kontrol negatif dan sama dengan kontrol positif adalah 50%. Dari analisis probit diperoleh nilai LC50 sebesar 43,709%, sedangkan untuk nilai LC90 sebesar 49,069%. Nilai LT50 dan LT90 dari penelitian ini adalah 1220,152 menit dan 1126,488 menit. Kesimpulan dari penelitian ini adalah ekstrak daun cengkeh berpotensi sebagai insektisida terhadap Ae. aegypti, dan konsentrasi ekstrak yang paling efektif adalah konsentrasi sebesar 50%.

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