Larvicidal and repellent activity of N-methyl-1-adamantylamine and oleic acid a major derivative of bael tree ethanol leaf extracts against dengue mosquito vector and their biosafety on natural predator

Author(s):  
Muthiah Chellappandian ◽  
Sengottayan Senthil-Nathan ◽  
Sengodan Karthi ◽  
Prabhakaran Vasantha-Srinivasan ◽  
Kandaswamy Kalaivani ◽  
...  
Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3844
Author(s):  
Sengodan Karthi ◽  
Karthic Uthirarajan ◽  
Vinothkumar Manohar ◽  
Manigandan Venkatesan ◽  
Kamaraj Chinnaperumal ◽  
...  

The larvicidal potential of crude leaf extracts of Rhizophora mucronata, the red mangrove, using diverse solvent extracts of the plant against the early fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquito vectors was analyzed. The acetone extract of R. mucronata showed the greatest efficacy: for Cx. quinquefasciatus (LC50 = 0.13 mg/mL; LC90 = 2.84 mg/mL), An. stephensi (LC50 = 0.34 mg/mL; LC90 = 6.03 mg/mL), and Ae. aegypti (LC50 = 0.11 mg/mL; LC90 = 1.35 mg/mL). The acetone extract was further fractionated into four fractions and tested for its larvicidal activity. Fraction 3 showed stronger larvicidal activity against all the three mosquito larvae. Chemical characterization of the acetone extract displayed the existence of several identifiable compounds like phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 1-hexyl-2-nitrocyclohexane, eicosanoic acid etc. Enzyme assay displayed that R. mucronata active F3-fractions exert divergent effects on all three mosquitos’ biochemical defensive mechanisms. The plant fractions displayed significant repellent activity against all the three mosquito vectors up to the maximum repellent time of 210 min. Thus, the bioactive molecules in the acetone extract of R. murconata leaves showed significant larvicidal and enzyme inhibitory activity and displayed novel eco-friendly tool for mosquito control.


1970 ◽  
Vol 43 (2) ◽  
pp. 251-258 ◽  
Author(s):  
LAM Khanam ◽  
MS Rahman ◽  
I Mahfuz

Repellent activity of different solvent extracts of Zingiber cassumunar rhizome and leaf against Tribolium castaneum and T. confusum were conducted. The results indicated that both the insect species were repelled by Z. cassumunar rhizome and leaf extracts. Among these, acetone extracts of rhizome showed highest repellency to T. castaneum than those of other extract tested. Petroleum ether extracts of Z. cassumunar leaf acted as repulsive to T. castaneum whereas other extracts exhibited weak to moderate effects to the beetles. All the extracts of leaf produced weak to moderate effects to T. confusum however, methanol extract of leaf showed little effect to the bettles. Key words: Zingiber cassumunar, Seed and leaf extracts, Repellency, Tribolium castaneum, T. confusum.DOI: 10.3329/bjsir.v43i2.969   Bangladesh J. Sci. Ind. Res. 43(2), 251-258, 2008


Author(s):  
Bapan Banik ◽  
Jayanta Barman ◽  
Manash Pratim Dutta ◽  
Nikita Bhowmick

Mosquito repellent is a product that is applied to the skin or other surfaces to deter insects (and arthropods in general) from landing or climbing on that surface. The interest in plant-based repellants has been revived seeing that the development of resistance, cross-resistance and possible toxicity hazards associated with synthetic insecticides and their rising cost. The present study aimed towards the development and evaluation of safe and efficient herbal mosquito repellent from leaf extracts of Azadirachta indica, Vitex negundo, and Ocimum gratissimum. Six different types of formulation (Type-1 to 6) were prepared using each plant extract and optimized ingredients. The mosquito repellent activity of the formulation was tested using the arm-in-cage method. One skin rubbed with a small quantity of formulation was exposed to 20 mosquitoes and the number of mosquitoes that aligned or biting the arm was recorded in each minute for five minutes. The most effective repellent activity of the Type2 formulation was 87.5% (Y-shaped Model) and 91.62% (Rectangular-shaped Model). The Type1, Type3, Type4, Type5 and Type6 formulation showed 66.62% , 58.25%, 68.75%, 70.75% and 62.5% repellent activity in Y shaped model and 66.62%, 62.5%, 70.75%, 83.25% and 62.5% repellent activity in Rectangular shaped model respectively. During these studies, we found that Hexane extract showed higher repellent activity. No allergic reactions were observed upon the skin. So, it is a safe product. The formulation was ecological, economical and highly efficient.


2021 ◽  
Vol 46 (3) ◽  
Author(s):  
Maureen Leyva ◽  
María del Carmen Marquetti ◽  
Domingo Montada ◽  
Gisel Morejón ◽  
Oriela Pino ◽  
...  

2012 ◽  
Vol 30 ◽  
pp. 25-28 ◽  
Author(s):  
M Abdullah ◽  
A Kumar Pk ◽  
DKMA Saleh ◽  
A R Khan ◽  
N Islam

The insecticidal and repellent activity tests of the chloroform extracts of fruit, leaf, root and stem of Urena sinuata L. against the red flour beetle Tribolium castaneum L. adults have been conducted. The leaf, root and stem extracts showed high toxicity by giving mortality of the beetles within 30 min. of exposure. The LD50 values for the 12, 24, 36 and 48h of exposures were 0.354, 0.262, 0.209 and 0.196mg cm2 for the fruit extract; 0.587, 0.413, 0.355 and 0.299mg cm2 for the leaf extract; 0.440, 0.389, 0.340 and 0.268mg cm2 for the root extract and 0.968, 0.661, 0.491 and 0.362mg cm2 for the stem extract. The insecticidal activity can be arranged in the order of fruit> root> leaf> stem extracts. The root and stem showed repellent activity against T. castaneum adults at P<0.01 and P<0.05 levels of significance, while the fruit and the leaf extracts did not show repellency at all. DOI: http://dx.doi.org/10.3329/ujzru.v30i0.10740 Univ. j. zool. Rajshahi Univ. Vol. 30, 2011 pp. 25-28


2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Suharno Zen

Dengue fever is an infectious disease caused by a virus and is transmitted by the Aedes aegypti mosquito. Dengue fever has not yet found a cure and the only prevention is through mosquito vector  control. Health Department policy to prevent and eradicate dengue fever today is to make eradication by means of mechanical, physical, chemical or biological. Biological is one of alternative  control of mosquito vectors that are safe for humans and the environment but still effective in suppressing the mosquito vector. One attempt to do that is by using animals to fight other creatures, or better known as biological control. In the present research was performed using fish of Betta spp, Cyprinus carpio and Oreochormis niloticus as a natural predator of the Aedes aegypti mosquito. The design of this research is  Completely Randomized Design (CRD) with three treatments are 3 types of fish with 5 times repeated. Data were analyzed with analysis of the range and tested further with the Smallest Real Differences Test (LSD) on the real level 5%. The result of this research is the ability Betta spp eating is higher than the Oreochromis niloticus and Cyprinus carpio by the number of larvae that consumed between 34.6 to 36.9 mosquito larvae tail. The ability to eat at various types of fish against mosquito larvae, influenced by several factors: the agressive, espesialisasi fish to food, the active duration and the amount of time the fish is active in 24 hours.


2017 ◽  
Vol 9 (4) ◽  
pp. 351-357 ◽  
Author(s):  
M. Nesa ◽  
S. Islam ◽  
S. K. Sharma ◽  
S. B. Rekha ◽  
J. Ferdous ◽  
...  

Petroleum (Pet.) ether, CHCl3 and CH3OH extracts of Cassia renigera Benth. were tested against Callosobruchus chinensis L. for dose-mortality and repellency; and against Sitophilus oryzae L. and Tribolium castaneum (Hbst.) for repellent activity test. The Pet. ether extract of leaf was found active against C. chinensis adults through dose mortality assay and the LD50 values established were 9.270, 4.932, 2.032, 1.721, 1.671, 1.543, 1.513, 1.503 and 1.478 mg cm-2 for 0.5, 6, 12, 18, 24, 30, 36, 42 and 48 h of exposure respectively. However, the leaf extracts of CHCl3 and CH3OH; as well as the Pet. ether, CHCl3 and CH3OH extracts of the stem-bark did not give mortality to the test organism. The CHCl3 extract of leaf showed repellent activity against Tribolium castaneum at 0.1% level of significance (P < 0.001), and the Pet. ether extracts of the leaf and the CH3OH extracts of the stem-bark offered repellency at 1% level of significance (P < 0.01) between dose levels. Only the CHCl3 extracts of the stem-bark gave repellency at 5% level of significance (P < 0.05), while the CH3OH extracts of leaf and Pet. ether extract of stem-bark did not show any significant repellency at all.


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