Effects of cell culture techniques on gene expression and cholesterol efflux in primary bovine mammary epithelial cells derived from milk and tissue

2012 ◽  
Vol 48 (9) ◽  
pp. 550-553 ◽  
Author(s):  
D. Sorg ◽  
A. Potzel ◽  
M. Beck ◽  
H. H. D. Meyer ◽  
E. Viturro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Epithelial Cells ◽  
Cholesterol Efflux ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Cell Culture Techniques ◽  
Bovine Mammary Epithelial Cells ◽  
Culture Techniques

scholarly journals  Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

2012 ◽  
Vol 57 (No. 10) ◽  
pp. 469-480 ◽  
Author(s):  
T. Sigl ◽  
H.H.D. Meyer ◽  
S. Wiedemann
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Milk Protein ◽  
Protein Gene ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Milk Protein Gene ◽  
Bovine Mammary Epithelial Cells ◽  
Milk Protein Genes ◽  
Milk Protein Gene Expression

&nbsp;The objective of the present study was to refine a previously developed method to isolate primary bovine mammary epithelial cells (pBMEC) from fresh milk. Using this method, it was tested whether the number of pBMEC and the relation of recovered pBMEC to total somatic cell count vary within the individual lactation stages. Furthermore, the expression levels of the milk protein genes during the first twenty weeks of lactation were determined by quantitative PCR method. A total number of 152 morning milk samples were obtained from twenty-four Holstein-Friesian cows during the first 20 weeks of lactation (day 8, 15, 26, 43, 57, 113, and 141 postpartum). Numbers of extracted pBMEC were consistent at all time-points (1.1 &plusmn; 0.06 to 1.4 &plusmn; 0.03 &times;10<sup>3</sup>/ml) and an average value of RNA integrity number (RIN) was 6.3 &plusmn; 0.3. Percentage of pBMEC in relation to total milk cells (2.0 &plusmn; 0.2 to 6.7 &plusmn; 1.0%) correlated with milk yield. Expression patterns of the casein genes alpha (&alpha;)<sub>S1</sub>, (&alpha;)<sub>S2</sub>, beta (&beta;), and kappa (&kappa;) (CSN1S1, CSN1S2, CSN2, CSN3, respectively) and the whey protein genes &alpha;-lactalbumin (LALBA) and progestagen-associated endometrial protein (PAEP; known as &beta;-lactoglobulin) were shown to be comparable, i.e. transcripts of all six milk protein genes were found to peak during the first two weeks of lactation and to decline continuously towards mid lactation. However, mRNA levels were different among genes with CSN3 showing the highest and LALBA the lowest abundance. We hypothesized that milk protein gene expression has a pivotal effect on milk protein composition with no influence on milk protein concentration. This paper is the first to describe milk protein gene expression during lactation in pBMEC collected in milk. Future studies will be needed to understand molecular mechanisms in pBMEC including regulation of expression and translation throughout lactation. &nbsp;

1082 Gene expression and secretion of chemerin in bovine mammary epithelial cells

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 519-519
Author(s):  
Y. Suzuki ◽  
S. Chiba ◽  
S. Haga ◽  
S. Roh
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk

2017 ◽  
Vol 53 (8) ◽  
pp. 706-720 ◽  
Author(s):  
Maria Hillreiner ◽  
Nadine I. Müller ◽  
Heiner M. Koch ◽  
Christiane Schmautz ◽  
Bernhard Küster ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
3D Cell Culture ◽  
Mammary Epithelial ◽  
Cell Culture Model ◽  
Bovine Mammary Epithelial Cells ◽  
Fresh Milk ◽  
Culture Model

Immunomodulatory molecules regulate adhesin gene expression in Staphylococcus aureus: Effect on bacterial internalization into bovine mammary epithelial cells

2019 ◽  
Vol 131 ◽  
pp. 15-21
Author(s):  
Minerva Frutis-Murillo ◽  
Marcelo Alejandro Sandoval-Carrillo ◽  
Nayeli Alva-Murillo ◽  
Alejandra Ochoa-Zarzosa ◽  
Joel E. López-Meza
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

FABP5 is a critical regulator of methionine- and estrogen-induced SREBP-1c gene expression in bovine mammary epithelial cells

2018 ◽  
Vol 234 (1) ◽  
pp. 537-549 ◽  
Author(s):  
Ping Li ◽  
Mengmeng Yu ◽  
Chengjian Zhou ◽  
Hao Qi ◽  
Xuepeng Wen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells
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