Evaluation of different conventional lipid extraction techniques’ efficiency in obtaining oil from oleaginous seeds

2020 ◽  
Author(s):  
Victor H. M. da Cruz ◽  
Rafael L. G. Priori ◽  
Patrícia D. S. Santos ◽  
Cintia S. F. Ferreira ◽  
Angélica de F. B. Piccioli ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Extraction Techniques

Microalgal Cell Disruption and Lipid Extraction Techniques for Potential Biofuel Production

2020 ◽  
pp. 129-147
Author(s):  
Ahasanul Karim ◽  
M. Amirul Islam ◽  
Zaied Bin Khalid ◽  
Che Ku Mohammad Faizal ◽  
Md. Maksudur Rahman Khan ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Cell Disruption ◽  
Biofuel Production ◽  
Extraction Techniques

scholarly journals Effect on the fatty acid profiles of a microalgae strain (Dunaliella tertiolecta) using different lipid extraction techniques

2020 ◽  
pp. 15-22
Author(s):  
Fidel Gómez-Cordova ◽  
Irma Palomares-Ruiz ◽  
David Santos-Ballardo ◽  
Nildia Mejias-Brizuela
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Physicochemical Parameters ◽  
Lipid Extraction ◽  
Dry Weight ◽  
Dunaliella Tertiolecta ◽  
Fatty Acid Profiles ◽  
Extraction Techniques ◽  
Weight Content

Biodiesel is subject of research because it is less polluting when used in pure form or mixed with petroleum diesel. Microalgae are now a material new in research for mass production of biodiesel, so the techniques used for cell growth, biomass extraction and lipid extraction influence the profile of fatty acids susceptible to transesterification and consequently the quality of biofuel. This work shows the effect on the fatty acid profile of a Dunaliella tertiolecta strain using two lipid extraction techniques. For this, the culture was carried out in the medium F/2, the recovery of the biomass was carried out by sedimentation-flocculation with NaOH and once dry it was subjected to extraction with solvent by Soxhlet and by ultrasound to obtain lipids under the methodology by Bligh & Dyer. The fatty acids were analyzed by gas chromatography and the profile is made up of saturated-monounsaturated-polyunsaturated fatty acids and shows differences regarding the presence-absence and dry weight content. This represents a contribution to analyze the effect on physicochemical parameters established in Mexican regulations regarding the quality of biodiesel and to determine the potential of the microalgae strain for such production.

scholarly journals Differential Thermal Isomerization: Its Role in the Analysis of Vitamin D3 in Foods

2020 ◽  
Vol 103 (2) ◽  
pp. 563-569
Author(s):  
Brendon D Gill ◽  
Grant A Abernethy ◽  
Harvey E Indyk ◽  
Jackie E Wood ◽  
David C Woollard
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Lipid Extraction ◽  
Internal Standard ◽  
Thermal Isomerization ◽  
Reliable Estimate ◽  
Vitamin D2 ◽  
Extraction Techniques ◽  
Internal Standards ◽  
Cold Saponification

Abstract Background For nutritional purposes, the measurement of vitamin D3 (defined as the sum of vitamin D3 and previtamin D3) is required to obtain an accurate and reliable estimate of its content in foods. An often neglected aspect in the development of methods for the analysis of vitamin D3 is accounting for any potential analytical bias in the results associated with differential thermal isomerization between previtamin D and vitamin D. Conclusions For LC-UV methods using a vitamin D2 internal standard, cold saponification, or direct lipid extraction techniques should be avoided, unless chromatographic separation of vitamin D2, vitamin D3, and their previtamin forms is achieved so that UV absorbance corrections can be made. For both LC-UV and LC-MS methods using calciferol internal standards, the simplest solution to avoid analytical bias due to the presence of previtamin D is to utilize heating conditions (typically during saponification) such that previtamin D and vitamin D in the sample and the internal standard reach an equivalent equilibrium state prior to instrumental analysis. Only under such circumstances is the integration of previtamin D unnecessary to obtain accurate results for vitamin D3. Highlights A detailed discussion of the quantitation of vitamin D3 in food with concise recommendations for avoiding measurement bias as a consequence of differential thermal isomerization.

scholarly journals Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 231 ◽  
Author(s):  
Julian Aldana ◽  
Adriana Romero-Otero ◽  
Mónica P. Cala
Keyword(s):  
Mass Spectrometry ◽  
Energy Homeostasis ◽  
Lipid Extraction ◽  
Structural Diversity ◽  
Extraction Yield ◽  
Mass Spectrometry Analysis ◽  
Extraction Techniques ◽  
Lipid Profiling ◽  
Spectrometry Analysis ◽  
Living Organisms

In recent years, high-throughput lipid profiling has contributed to understand the biological, physiological and pathological roles of lipids in living organisms. Across all kingdoms of life, important cell and systemic processes are mediated by lipids including compartmentalization, signaling and energy homeostasis. Despite important advances in liquid chromatography and mass spectrometry, sample extraction procedures remain a bottleneck in lipidomic studies, since the wide structural diversity of lipids imposes a constrain in the type and amount of lipids extracted. Differences in extraction yield across lipid classes can induce a bias on down-stream analysis and outcomes. This review aims to summarize current lipid extraction techniques used for untargeted and targeted studies based on mass spectrometry. Considerations, applications, and limitations of these techniques are discussed when used to extract lipids in complex biological matrices, such as tissues, biofluids, foods, and microorganisms.

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