scholarly journals Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

2015 ◽  
Vol 37 (11) ◽  
Author(s):  
Kamil Frankowski ◽  
Emilia Wilmowicz ◽  
Agata Kućko ◽  
Agnieszka Zienkiewicz ◽  
Krzysztof Zienkiewicz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abscission Zone ◽  
Lupinus Luteus ◽  
Generative Organs

Increased hydrolase gene expression and hydrolase activity in the abscission zone involved in chilling-induced abscission of Dendrobium flowers

2016 ◽  
Vol 117 ◽  
pp. 217-229 ◽  
Author(s):  
Saovalak Phetsirikoon ◽  
Robert E. Paull ◽  
Nancy Chen ◽  
Saichol Ketsa ◽  
Wouter G.van Doorn
Keyword(s):  
Gene Expression ◽  
Abscission Zone ◽  
Hydrolase Activity

scholarly journals Fruit drop: II. Biological background of flower and fruit drop

2006 ◽  
Vol 12 (3) ◽  
Author(s):  
J. Racskó ◽  
M. Soltész ◽  
Z. Szabó ◽  
J. Nyéki
Keyword(s):  
Water Stress ◽  
Abscission Zone ◽  
Leaf Abscission ◽  
Fruit Drop ◽  
Windy Weather ◽  
Environmental Causes ◽  
Generative Organs

The most important components of fruit drop are: the rootstock, the combination of polliniser varieties, the conditions depending of nutrition, the extent and timing of the administration of fertilisers, the moments of water stress and the timing of agrotechnical interventions. Further adversities may appear as flushes of heat and drought, the rainy spring weather during the blooming period as well as the excessive hot, cool or windy weather impairing pollination, moreover, the appearance of diseases and pests all influence the fate of flowers of growing and become ripe fruits. As generally maintained, dry springs are causing severe fruit drop. In analysing the endogenous and environmental causes of drop of the generative organs (flowers and fruits), the model of leaf abscission has been used, as a study of the excised, well defined abscission zone (AZ) seemed to be an adequate approach to the question. Comparing the effects active in the abscission of fruit with those of the excised leaf stem differences are observed as well as analogies between the anatomy and the accumulation of ethylene in the respective abscission tissues.

The influence of abscisic acid on the ethylene biosynthesis pathway in the functioning of the flower abscission zone in Lupinus luteus

2016 ◽  
Vol 206 ◽  
pp. 49-58 ◽  
Author(s):  
Emilia Wilmowicz ◽  
Kamil Frankowski ◽  
Agata Kućko ◽  
Michał Świdziński ◽  
Juan de Dios Alché ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Ethylene Biosynthesis ◽  
Abscission Zone ◽  
Lupinus Luteus ◽  
Biosynthesis Pathway ◽  
Flower Abscission

Gibberellic acid affects the functioning of the flower abscission zone in Lupinus luteus via cooperation with the ethylene precursor independently of abscisic acid

2018 ◽  
Vol 229 ◽  
pp. 170-174 ◽  
Author(s):  
Katarzyna Marciniak ◽  
Agata Kućko ◽  
Emilia Wilmowicz ◽  
Michał Świdziński ◽  
Krzysztof Przedniczek ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Abscission Zone ◽  
Lupinus Luteus ◽  
Ethylene Precursor ◽  
Flower Abscission

scholarly journals Functional Analysis of the Lupinus luteus Cyclophilin Gene Promoter Region in Lotus japonicus

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 435
Author(s):  
Katarzyna Nuc ◽  
Przemysław Olejnik ◽  
Mirella Samardakiewicz ◽  
Przemysław Nuc
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Promoter Region ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Gus Expression ◽  
Lupinus Luteus ◽  
Cis Elements ◽  
Specific Element ◽  
Promoter Deletion

Functional analysis of promoter sequences is important to understand the regulation of gene expression. This study aimed to investigate the promoter region of the Lupinus luteus cytoplasmic cyclophilin gene (LlCyP; AF178458). After bioinformatic analysis, four promoter deletion fragments were fused to the β-glucuronidase reporter gene. We used Lotus japonicus as a model plant. After Agrobacterium rhizogenes transformation of L. japonicus, only the longest promoter region (−1055 bp to ATG) supported the β-glucuronidase expression in root nodule parenchyma. Putative cis-elements located between −1055 and −846 bp were subjected to site-directed mutagenesis. Mutations incorporated in the TGATT and AGATT motifs (cytokinin response) abolished GUS expression in nodules, but the mutated AAAGAT motif (OSE, organ-specific element) still activated the GUS expression in root nodules, mainly in cells surrounding the vascular bundle. Promoter deletion and mutation experiments suggest that cis-elements responsible for gene expression in the nodule are located in the region spanning from −1055 to −846 bp. We constructed a deletion fragment, in which the DNA sequence located between −822 and −198 bp was removed (pCYPMG). The promoter region arranged in the pCYPMG supports the expression in the parenchyma of L. japonicus nodules, but it is lower than the whole promoter region. The obtained results may be useful for transgene expression in determinate and indeterminate root nodules.

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

2015 ◽  
Vol 290 (5) ◽  
pp. 1991-2006 ◽  
Author(s):  
Chunzhen Cheng ◽  
Lingyun Zhang ◽  
Xuelian Yang ◽  
Guangyan Zhong
Keyword(s):  
Gene Expression ◽  
Citrus Fruit ◽  
Abscission Zone ◽  
Calyx Abscission

scholarly journals Cellulase Activity and Gene Expression in Citrus Fruit Abscission Zones during and after Ethylene Treatment

1998 ◽  
Vol 123 (5) ◽  
pp. 781-786 ◽  
Author(s):  
William C. Kazokas ◽  
Jacqueline K. Burns
Keyword(s):  
Gene Expression ◽  
Cellulase Activity ◽  
Storage Period ◽  
Abscission Zone ◽  
Cellulase Gene ◽  
Immature Fruit ◽  
Ethylene Treatment ◽  
Fruit Abscission ◽  
Valencia Orange ◽  
Low Levels

Mature and immature `Valencia' orange [Citrus sinensis (L.) Osbeck] and immature `Valencia' orange and `Tahiti' lime (Citrus latifolia Tan.) fruit with attached pedicels were treated with 8 μL·L-1 ethylene for periods up to 24 hours. Endo-β-1,4-glucanase (cellulase) activity and gene expression were determined in fruit abscission zones during and after ethylene exposure. Cellulase activities were not detected in mature `Valencia' orange and immature `Tahiti' lime fruit abscission zones immediately following harvest and after 6 hours of ethylene treatment. After 12 hours of ethylene treatment, cellulase activity increased and was highest after 24 hours. Cellulase gene expression preceded the rise in cellulase activity and was detectable after 6 hours of ethylene treatment, but then declined after 12 hours. Following transfer to air storage, abscission zone cellulase activity in mature `Valencia' fruit remained high, whereas activity in immature `Tahiti' fruit declined. After 168 hours air storage, activity in abscission zones of mature `Valencia' fruit decreased slightly, but activity in abscission zones of immature `Tahiti' lime fruit increased to the highest level. Expression of abscission zone cellulase gene Cel-a1 in abscission zones of mature `Valencia' fruit markedly increased after transfer to air and was highest after 48 hours air storage. Cel-a1 expression returned to low levels after 168 hours of air storage, but expression of cellulase gene Cel-b1 remained at low levels throughout the air storage period. Expression of Cel-a1 and Cel-b1 declined in fruit abscission zones of immature `Valencia' and `Tahiti' lime fruit upon transfer to air. After 168 hours of air storage, expression of Cel-a1 again rose to high levels but Cel-b1 remained low. The results suggest that differences in cellulase activity and gene expression measured in mature and immature fruit abscission zones during ethylene treatment and subsequent air storage may, in part, explain the differential response of mature and immature fruit to abscission agents.

scholarly journals Early gene expression events in the laminar abscission zone of abscission-promoted citrus leaves after a cycle of water stress/rehydration: involvement of CitbHLH1

2012 ◽  
Vol 63 (17) ◽  
pp. 6079-6091 ◽  
Author(s):  
Javier Agustí ◽  
Jacinta Gimeno ◽  
Paz Merelo ◽  
Ramón Serrano ◽  
Manuel Cercós ◽  
...  
Keyword(s):  
Gene Expression ◽  
Water Stress ◽  
Abscission Zone ◽  
Early Gene ◽  
Early Gene Expression ◽  
Citrus Leaves

scholarly journals Ethylene-dependent effects on generative organ abscission of Lupinus luteus

2017 ◽  
Vol 86 (1) ◽  
Author(s):  
Kamil Frankowski ◽  
Agata Kućko ◽  
Agnieszka Zienkiewicz ◽  
Krzysztof Zienkiewicz ◽  
Juan de Dios Alché ◽  
...  
Keyword(s):  
Physiological Response ◽  
Considerable Increase ◽  
Separation Process ◽  
Abscission Zone ◽  
Lupinus Luteus ◽  
Flower Abortion ◽  
Dividing Cells ◽  
Generative Organ ◽  
Flower Abscission ◽  
Acc Content

The abscission of certain organs from the plant is part of the fulfilment of its developmental programs. The separation process occurs in a specialized abscission zone usually formed at the base of detached organ. The changing level of phytohormones, particularly ethylene, is the element responsible for coordinating anatomical and physiological transformation that accompanies organ abscission. The application of ethylene (ET) on <em>Lupinus luteus</em> stimulates flower abortion. However, the treatment with 1-aminocyclopropane-1-carboxylic acid (ACC) – direct ET precursor – does not cause such a strong physiological response. In turn, when applied on the pedicels both ET biosynthesis (2-aminoethoxyvinylglycine; AVG) and action (norbornadiene; NBD) inhibitors reversed the stimulatory effect of ET on generative organ separation. In order to determine ET role in the flower abscission process in <em>L. luteus</em>, we identified the sequences coding for synthase (<em>LlACS</em>) and oxidase (<em>LlACO</em>) of ACC and measured their expression levels. Abscission zone activation is accompanied by a considerable increase both in <em>LlACS</em> and <em>LlACO</em> cDNAs and also ACC content, which is specifically localized in the dividing cells at the base of the flower being detached. Obtained results suggest that ET is a strong stimulator of flower abortion in <em>L. luteus</em>.

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