Improving rapeseed (Brassica napus L.) plant performance by exogenous salicylic acid and putrescine under gradual water deficit

2019 ◽  
Vol 41 (12) ◽  
Author(s):  
Kazem Ghassemi-Golezani ◽  
Hamed Mabudi Bilasvar ◽  
Adel Dabbagh Mohammadi Nassab
2015 ◽  
Vol 67 (2) ◽  
pp. 397-404 ◽  
Author(s):  
Ghader Habibi

The present research was designed to determine the effects of exogenous salicylic acid (SA), selenium (Se) and silicon (Si) on the resistance of canola (Brassica napus L. cv Okapi) seedlings to salt stress. Foliar application of SA (0.1 mM) in canola plants under drought stress for 25 days exhibited a significantly positive effect on shoot dry mass and raised the levels of total chlorophyll as well as boosting the activity of superoxide dismutase (SOD) and catalase (CAT). In addition, soil application of silicon (0.35 g Na2SiO3/kg soil) had ameliorative effects on canola root growth under drought. It is concluded that SA and Si enhanced the salt tolerance of canola by protecting the cell membrane against lipid peroxidation. However, the foliar application of Se (10 mg/l) had no ameliorative effects on canola growth and antioxidant capacity under drought stress, as could be judged by accumulation of malondialdehyde (MDA).


2016 ◽  
Vol 8 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Hamed KESHAVARZ ◽  
Seyed Ali Mohammad MODARRES SANAVY ◽  
Ramin SADEGH GHOL MOGHADAM

In this study the effect of foliar application of salicylic acid on the chlorophyll content, antioxidant enzymes activity, and the content of solute protein and proline were investigated in two canola varieties (Brassica napus L., cv ‘RGS’ and ‘Licord’) leaves during 0, 24, and 48 hours after salicylic acid treatment. The results showed that the content of total chlorophyll was decreased in ‘RGS’ cultivar during the experiment and this process was related with increasing of salicylic acid concentration. The activity of superoxide dismutase, peroxidase, and also lipid peroxidation were increased significantly after 48 hours compared with the first day. The results of catalase activity showed that, this trait was decreased 24 hours after salicylic acid treatment and this decrease was related with salicylic concentration. The content of protein in both cultivars slightly changed and plants treated with salicylic acid had more protein content, by contrast proline was greatly affected by salicylic acid treatment and its content was the highest 24 hours after treatment. According to the present findings the application of salicylic acid has useful effects on the biochemical traits of Brassica napus cultivars. Therefore it may be effective for the improvement of plant growth in cold regions.


2020 ◽  
Vol 33 (1) ◽  
pp. 13-20
Author(s):  
Muhammad Awais Ghani ◽  
Muhammad Mehran Abbas ◽  
Basharat Ali ◽  
Khurram Ziaf ◽  
Muhammad Azam ◽  
...  

Tri-genomic Brassica napus L.wasdeveloped by the cross between Brassica napusand Brassica nigra. The crop is animportant source of vegetable seed oil in Pakistan,after cotton. The low oilseed rape yield is attributed to high temperature in the production zones. Interspecific hybridization using these two speciescan be helpful to produce heat resistant hybrids. On the other hand, it has been found that foliar application of different plant growth regulators can be used to reduce the heat stress in Brassica. The objectiveof this studywas to test the response of three different tri-genomic hybrids to high temperature stressat seedling stage. Seedlings were foliar sprayed with 0.13 mM salicylic acid (SA) prior to exposure tohigh temperatureat two true leaf stage. The plants were harvested after 30 days of sowing for growth and biochemical analysis. Plants ofV38 showed the highest values for all morphological traits and biochemical activities among the threehybrids. In general, plants exposed to the temperature stress exhibited a significant decline in growth, chlorophyll content and enzyme activity.Foliar application of SA significantly improved leaf and root biomass under heat stress.Further, antioxidativeenzyme activities significantly increased in response to SA either compared to control or to plants exposed to temperature stress.It is concluded thatapplication of salicylic acid elevated activity of antioxidative enzymes and was helpful in mitigating the detrimental effects of high temperature inoil seed rape.


Author(s):  
Mahdi Khozaei ◽  
Shiva Boroumand Jazi

Oilseed plant, Brassica napus L. seedlings grown in hydroponic condition with different concentrations of Pb were treated with salicylic acid (SA) to investigate the role of exogenous salicylic acid in alleviating lead toxicity on biochemical and physiological activities of the plant. The results showed that application of different concentrations of Pb increased soluble sugars and reduced carbohydrate levels significantly in roots and shoots of the plants. The stress induced by application of Pb triggered significant inhibitory effects on growth and chlorophyll synthesis induced on the production of protein and proline and enhanced the levels of antioxidant activity. Salicylic acid (SA) treated plants showed alleviation increasing total dry mass, leaf area, shoot and root length as well as leaf total chlorophyll content in responses to Pb stress. Results revealed the importance of salicylic acid (SA) activity in enabling plants to reduce the soluble sugars and increase of insoluble sugar in heavy -metal-stressed plants. The content of proline and proteins were also reduced in plants were treated with salicylic acid. Our data provide evidence that salicylic acid treatment decreased the activity of antioxidant enzymes in plants were exposed to different levels of Pb.


2011 ◽  
Vol 3 (3) ◽  
pp. 120-125 ◽  
Author(s):  
Mohammad HOSSEINI ◽  
Payman HASSIBI

Water deficit stress considered as one of the most important limiting factors for oil seed canola (Brassica napus L.) growth and productivity in Iran. To evaluate the effects of water deficit stress on some qualitative and quantitative characteristics of canola cultivars, this experiment in a greenhouse trial carried out as factorial based on completely randomized design with three replications in Shahid Chamran University of Ahwaz (Iran). Canola cultivars, including ‘Hyola 308’, ‘Hyola 401’ and ‘RGS 003’ as first factor, and the second one was three levels of water deficit stress, including stress at early stem elongation stage to early flowering (D1), early flowering stage to early emergence of sacs (D2), beginning of stem elongation stage to early emergence of sacs (D3) and normal irrigation (C, as check). Results showed that the interaction between water deficit stress and cultivars affected biological yield, seed oil yields and harvest index (p≤0.01), dry matter and economic yield (p≤0.05). Water deficit stress reduced grain oil yields. ‘Hyola 308’ under stress at beginning stem elongation stage to early flowering had the lowest oil yields (1.1 g plants-1) and ‘Hyola 401’ under non-stress conditions showed highest oil yields (4.3 g plants-1). The decrease of oil yields at the flowering stage to stem elongation stage was more than the other stages. In addition, water deficit stress reduced harvest index in the three stress levels due to reduced economic yield and reduced biological yield. Stress susceptibility index for ‘Hyola 401’ at the beginning of stem elongation stage to early emergence of sacs was 0.914 and the ‘Hyola 308’ showed 1.12 at the beginning of stem elongation stage to early emergence of sacs respectively, which it can implies that ‘Hyola 308’ is more sensitive than ‘Hyola 401’ to water deficit stress.


2015 ◽  
Vol 52 (1-2) ◽  
pp. 19-36 ◽  
Author(s):  
Roya Razavizadeh

Abstract The effect of salicylic acid (SA) on the salt (NaCl) tolerance mechanism was studied in canola plants (oilseed rape, Brassica napus L.) by molecular and physiological experiments in plant tissue culture. Seeds of B. napus ‘Ocapy’ were germinated at 0, 50, and 100 mM NaCl on Murashige and Skoog (MS) medium containing different levels (0, 2, and 5 μM) of SA for 4 weeks. Total chlorophyll, carotenoid, and flavonoid content increased in response to interactive effects of SA and NaCl treatments at some concentrations. Proline content was increased under salt and SA treatments in shoot and root tissues. Salt alone and in combination with SA increased the total soluble protein content of shoots only, while the different concentrations of SA in the culture media affected variously the total soluble protein content. Protein patterns of shoots and roots showed some remarkable differences, based on gel electrophoresis and the consequent analysis of bands by ImageJ program. The relative expression of 15 and 12 protein bands in shoots and roots, respectively, differed under the applied treatments. In addition, the protein profile indicated that salinity and SA regulate the expression of salt-stress-inducible proteins as well as induced de novo synthesis of specific polypeptides. The findings may help to explain the salt tolerance mechanisms and to produce salt-tolerant canola plants.


Sign in / Sign up

Export Citation Format

Share Document