scholarly journals Evaluation of Microencapsulated Synbiotic Preparations Containing Lactobionic Acid

2021 ◽  
Author(s):  
Kamila Goderska ◽  
Patryk Kozłowski
Keyword(s):  
In Vitro Model ◽  
Bifidobacterium Bifidum ◽  
Probiotic Bacteria ◽  
Lactobionic Acid ◽  
Adverse Conditions ◽  
Encapsulation Process ◽  
Vitro Model ◽  
Human Digestive Tract ◽  
Different Strains

AbstractThe aim of this paper was to assess the prebiotic properties of lactobionic acid in the human gastrointestinal model. Five different strains of probiotic, or potentially probiotic, bacteria were used in the microencapsulation process; these were Lactobacillus casei Shirota, Lactococcus lactis ATCC1, Lactobacillus fermentum, Bifidobacterium bifidum DSM 20456, and Bifidobacterium bifidum DSM 20082. Starch with a concentration of 4% (w/v) and a degree of substitution of 0.03 was used to produce the microcapsules. The alginian microcapsules we produced functioned as a protective barrier for the probiotic microorganisms closed in them, protecting them from adverse conditions in the human digestive tract. The microorganisms could thus survive the encapsulation process and the in vitro model digestion process while retaining the ability to produce biomass. Factors such as pH and time affect the solution of alginate microcapsules. The capsule solution began when a pH of 7.4 was reached; this corresponded to pH in the target probiotic site, an in vitro model of the colon. The capsules had completely dissolved after 24 h of digestion at a pH of 8. The addition of lactobionic acid stimulated the growth of probiotic and potentially probiotic bacteria, thus confirming its prebiotic properties.

scholarly journals Immobilisation of bifidobacteria in biodegradable food-grade microparticles

2019 ◽  
pp. 74-83 ◽  
Author(s):  
Tatyana Voblikova ◽  
Saverio Mannino ◽  
Lyudmila Barybina ◽  
Vladimir Sadovoy ◽  
Anatoly Permyakov ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Control Sample ◽  
Test Sample ◽  
Plate Count ◽  
Modified Method ◽  
New Type ◽  
Vitro Model ◽  
Animal Mass ◽  
Human Digestive Tract

The present research features a natural polymer that can be used for immobilisation of bifidobacteria as well as a method of immobilisation. We described a modified method of microencapsulation of probiotics using sodi- um alginate. The experiment studied the effect of encapsulation on probiotic stability and involved an in vitro model of human digestive tract. The test sample of microencapsulated Bifi obacterium bifi um 791 showed a decrease in the activity from 3.0×107 to 2.2×105 CFU/ml in a mouse model with pH 1.2. By contrast, the control sample, unprotected by biodegradable polymer microcapsules, demonstrated a higher death rate of bifidobacteria: from 2.6×108 CFU/ml to 5.0×103 CFU/ml. The control sample demonstrated the same downward trend in in vitro gastrointestinal models with pH values of 4.5, 6.8, 7.2, and 5.8. Because the total plate count fell down to 4.0l g CFU/ml in acidity gradients, the titres of the initial microencapsulated biomass had to be increased up to > 109 CFU/ml. According to the results of scanning electron microscopy, the new type of microcapsules obtained by using a resistant starch had a closed sur- face. Prebiotics increased the resistance of bacteria to low pH and bile salts. Bifidobacteria encapsulated with natural biodegradable polymers proved to be well-tolerated and harmless for mice. The experiment revealed that biodegrad- able polymer microcapsules did not cause any chronic or acute toxicity when administered orally at 2×107 CFU per 1 gram of animal mass. The microcapsules demonstrated neither dermonecrotic properties nor any irritant effect on the ocular mucosa and, thus, can be used for food enforcement.

scholarly journals Synbiotic-Derived Metabolites Reduce Neuroinflammatory Symptoms of Alzheimer's Disease

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1578-1578
Author(s):  
Giulio Pasinetti
Keyword(s):  
Gastrointestinal Tract ◽  
Blood Brain Barrier ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Probiotic Bacteria ◽  
Amyloid Aggregation ◽  
Human Gastrointestinal Tract ◽  
Metabolite Production ◽  
Vitro Model

Abstract Objectives Synbiotics, the combination of probiotics and prebiotics, can be designed to produce specific bioavailable metabolites that penetrate the blood-brain-barrier and reduce neuropathologies associated with Alzheimer's Disease (AD). We designed an in vitro model of the human gastrointestinal tract that can be used to optimize the production of metabolites from synbiotic formulations using machine learning algorithms that can then be tested in animal models of AD. Our objective is to optimize/characterize a synbiotic's metabolite production using an in vitro bioreactor as a therapeutic tool against AD. We hypothesize that a synbiotic designed towards the production of specific brain-bioactive metabolites will synergistically mitigate the neuro- and systemic-pathologies associated with AD. Methods Using the in vitro model of the human gastrointestinal tract, we have used this optimized combination of polyphenols and tested 15 combinations of 6 probiotic bacteria as a synbiotic to optimize metabolite production using a multivariate regression algorithm (MARS) and found that three probiotic bacteria, Lactobacillus plantarum, Bifidobacterium infantis and L. salivarius together synergistically improve the production of brain bioavailable metabolites including quercetin, kamferin, 4-hydroxyphenylpyruvic acid and 4-hydroxyphenylacetic acid. Results We have found that two polyphenolic metabolites 3-hydroxybenzoic acid and 3-(3’-hydroxyphenyl) propionic acid derived from a grapeseed polyphenolic extract (GSPE) can penetrate the blood-brain-barrier and in vitro, inhibit the aggregation of amyloid plaques and tau fibrils. Increasing the diversity of the polyphenolic pool by adding a concord grape extract and resveratrol to the GSPE, we provided greater protection against cognitive impairment and amyloid aggregation in an AD mouse model than the components alone. Conclusions In vitro studies have confirmed that these metabolites have potent anti-inflammatory activity. This synbiotic combining potent grape-derived polyphenolic precursors with bioactive probiotic bacteria has the potential to slow the progression and treat AD by synergistically targeting multiple of its neuropathologies including inflammation, amyloid aggregation and tau fibril formation. Funding Sources These studies were funded by the NCCIH P50 AT008661 Center.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model
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