Saccharomyces Cerevisiae as an Untapped Source of Fungal Chitosan for Antimicrobial Action

Abstract Despite being widely available, Saccharomyces cerevisiae has not been widely explored for direct extraction of chitosan biopolymer for antimicrobial applications. In our study, S. cerevisiae from Baker’s yeast and Aspergillus niger from moldy onion extracts are studied as alternative sources of chitosan; and S cerevisiae chitosan tested for antimicrobial efficacy. The properties of S. cerevisiae chitosan are compared with moldy onion chitosan and shrimp chitosan extracted from shrimp shells. Chitosan extracted from S. cerevisiae is tested for antimicrobial efficacy against Staphylococcus Aureus. The maximum yields of fungal chitosan are 20.85 ± 0.35 mg/g dry S. cerevisiae biomass at 4th day using a culture broth containing sodium acetate, and 16.15 ± 0.95 mg/g dry A. niger biomass at 12th day. The degree of deacetylation (DD%) of the extracted fungal chitosan samples from S. cerevisiae and A. niger are found to be 63.4%, and 61.2% respectively, using Fourier Transform Infrared Spectroscopy. At a concentration of 2 g/L, S. cerevisiae chitosan shows the maximum inhibition zone diameter of 15.48 ± 0.07 mm. Baker’s yeast S cerevisiae biomass and A. niger from moldy onions has not been previously explored as a source of extractible fungal chitosan. This study gives insight that S. cerevisiae and A. niger from agricultural or industrial wastes could be a potential biomass source for production of the chitosan biopolymer. The S. cerevisiae chitosan displayed effective antimicrobial properties against S aureus , indicating the viablitiy of S cerevisae as a resource for extraction of high-quality chitosan.

Download Full-text

Synergistic antimicrobial action of phyco-synthesized silver nanoparticles and nano-fungal chitosan composites against drug resistant bacterial pathogens

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2020.1796787 ◽

2020 ◽

Vol 34 (1) ◽

pp. 631-639

Author(s):

Mohammed S. Alsaggaf ◽

Ahmed A. Tayel ◽

Mousa A. Alghuthaymi ◽

Shaaban H. Moussa

Keyword(s):

Silver Nanoparticles ◽

Bacterial Pathogens ◽

Antimicrobial Action ◽

Drug Resistant ◽

Fungal Chitosan

Download Full-text

Comparative analysis and antimicrobial action of some essential oils from plants

BIO Web of Conferences ◽

10.1051/bioconf/20213001011 ◽

2021 ◽

Vol 30 ◽

pp. 01011

Author(s):

Monica Mironescu ◽

Cecilia Georgescu

Keyword(s):

Saccharomyces Cerevisiae ◽

Comparative Analysis ◽

Essential Oils ◽

Volatile Oil ◽

Antimicrobial Action ◽

Mentha Piperita ◽

Inhibition Rate ◽

Origanum Vulgare ◽

Aerial Parts ◽

Main Components

In this research, essential oils (EOs) were extracted through steaming from aerial parts of three plants: basil (Ocinum basilicum L.), peppermint (Mentha piperita L.) and oregano (Origanum vulgare L.) form Sibiu County, Romania. The GC-MS analysis indicated that eugenol and apiol were the major compounds in the basil EO. Timol, ɣ-terpinene, and p-cymene were the major compounds in the volatile oil extracted from oregano and menthol and methone the main components in the peppermint EO. In the first 48 h of analysis, basil EO inhibition action was the highest against Salmonella anatum (48%), followed by Aspergillus niger (26%), Bacillus cereus (21%) and Saccharomyces cerevisiae (15%); this action was maintained for 120 h for all samples. In the first 48 h, the peppermint EO showed the highest inhibition rate (50%) on B. cereus, followed by A. niger (45%), S. cerevisiae (20%) and no action against Salmonella; this action varied after 72h and 120 h by slightly increasing in case of B. cereus, strongly decreasing in case of A. niger and remaining constant in case of S. cerevisiae. Oregano EO showed a very small antimicrobial action, only on S. anatum (12%) and this action was maintained for 120 h.

Download Full-text