Studies on Regulation of Global Protein Profile and Cellular Bioenergetics of Differentiating SH-SY5Y Cells

The goal of diabetes treatment is primarily to save life and alleviate symptoms and secondary to prevent long-term diabetic complications resulting from hyperglycemia. Thus, our present investigation was designed to evaluate the hepato-renal protective effects of gallic acid and p-coumaric acid in nicotinamide/streptozotocin (NA/STZ)-induced diabetic rats. Experimental type 2 diabetes was induced by a single intraperitoneal (i.p.) injection of STZ (65 mg/kg b.wt.), after 15 min of i.p. injection of NA (120 mg/kg b.wt.). Gallic acid and p-coumaric acid were orally administered to diabetic rats at a dose of 20, 40 mg/kg b.wt./day, respectively, for 6 weeks. Body weight, serum glucose, protein profile, liver function enzymes and kidney function indicators was assayed. Treatment with either gallic acid or p-coumaric acid significantly ameliorated the elevated levels of glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and uric acid. Both compounds were also found to restore total protein, albumin, and globulin as well as body weight of diabetic rats to near normal values. It can conclude that both gallic acid and p-coumaric acid have potent hypoglycemic and hepato-renal protective effects in diabetic rats. Therefore, our results suggest promising hypoglycemic agents that can attenuate the progression of diabetic hepatopathy and nephropathy.

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Mutans Streptococci counts from saliva and its protein profile in early childhood caries

Interventional Medicine and Applied Science ◽

10.1556/1646.10.2018.42 ◽

2018 ◽

Vol 10 (4) ◽

pp. 222-225 ◽

Cited By ~ 1

Author(s):

Endang W. Bachtiar ◽

Ferry P. Gultom ◽

Atika Rahmasari ◽

Boy M. Bachtiar

Keyword(s):

Early Childhood ◽

Early Childhood Caries ◽

Protein Profile ◽

Mutans Streptococci ◽

Childhood Caries

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ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

Proteome Science ◽

10.1186/s12953-021-00170-2 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Rong Zhang ◽

Weitao Jiang ◽

Xin Liu ◽

Yanan Duan ◽

Li Xiang ◽

...

Keyword(s):

Fusarium Moniliforme ◽

Abiotic Factors ◽

Fusarium Verticillioides ◽

Protein Profile ◽

Sugar Metabolism ◽

Differentially Expressed ◽

Pcr Analysis ◽

Differentially Expressed Proteins ◽

Apple Replant Disease ◽

Qrt Pcr

Abstract Background Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp. in ARD was further clarified. Methods In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. The differentially expressed protein was validated by qRT-PCR analysis. Results A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P< 0.05) differences. In-depth data analysis revealed that 59 proteins were found with more than 1.5 times (P< 0.05) differences, and most proteins were consistent with the result of qRT-PCR. Differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways. Conclusions This study is the first to perform quantitative proteomic investigation by iTRAQ labeling and LC-MS/MS to identify differentially expressed proteins in F. moniliforme under phloridzin conditions. The results confirmed that F. moniliforme presented a unique protein profile that indicated the adaptive mechanisms of this species to phloridzin environments. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.

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Synovial Tissue Proteins and Patient-Specific Variables as Predictive Factors for Temporomandibular Joint Surgery

Diagnostics ◽

10.3390/diagnostics11010046 ◽

2020 ◽

Vol 11 (1) ◽

pp. 46

Author(s):

Mattias Ulmner ◽

Rachael Sugars ◽

Aron Naimi-Akbar ◽

Nikolce Tudzarovski ◽

Carina Kruger-Weiner ◽

...

Keyword(s):

Temporomandibular Joint ◽

Synovial Tissue ◽

Surgical Outcome ◽

Clinical Decision Making ◽

Protein Profile ◽

Strong Association ◽

Tissue Inhibitor Of Metalloproteinase ◽

Patient Specific ◽

Disc Displacement ◽

Patient Reported

Our knowledge of synovial tissues in patients that are scheduled for surgery as a result of temporomandibular joint (TMJ) disorders is limited. Characterising the protein profile, as well as mapping clinical preoperative variables, might increase our understanding of pathogenesis and forecast surgical outcome. A cohort of 100 patients with either disc displacement, osteoarthritis, or chronic inflammatory arthritis (CIA) was prospectively investigated for a set of preoperative clinical variables. During surgery, a synovial tissue biopsy was sampled and analysed via multi-analytic profiling. The surgical outcome was classified according to a predefined set of outcome criteria six months postoperatively. Higher concentrations of interleukin 8 (p = 0.049), matrix metalloproteinase 7 (p = 0.038), lumican (p = 0.037), and tissue inhibitor of metalloproteinase 2 (p = 0.015) were significantly related to an inferior surgical outcome. Several other proteins, which were not described earlier in the TMJ synovia, were detected but not related to surgical outcome. Bilateral masticatory muscle palpation pain had strong association to a poor outcome that was related to the diagnoses disc displacement and osteoarthritis. CIA and the patient-reported variable TMJ disability might be related to an unfavourable outcome according to the multivariate model. These findings of surgical predictors show potential in aiding clinical decision-making and they might enhance the understanding of aetiopathogenesis in TMJ disorders.

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Discovery of fungal surface NADases predominantly present in pathogenic species

Nature Communications ◽

10.1038/s41467-021-21307-z ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Øyvind Strømland ◽

Juha P. Kallio ◽

Annica Pschibul ◽

Renate H. Skoge ◽

Hulda M. Harðardóttir ◽

...

Keyword(s):

Cell Death ◽

Aspergillus Fumigatus ◽

Neurospora Crassa ◽

Binding Site ◽

Host Cell ◽

Nicotinamide Adenine Dinucleotide ◽

Catalytic Mechanism ◽

Pathogenic Species ◽

X Ray ◽

Cellular Bioenergetics

AbstractNicotinamide adenine dinucleotide (NAD) is a key molecule in cellular bioenergetics and signalling. Various bacterial pathogens release NADase enzymes into the host cell that deplete the host’s NAD+ pool, thereby causing rapid cell death. Here, we report the identification of NADases on the surface of fungi such as the pathogen Aspergillus fumigatus and the saprophyte Neurospora crassa. The enzymes harbour a tuberculosis necrotizing toxin (TNT) domain and are predominately present in pathogenic species. The 1.6 Å X-ray structure of the homodimeric A. fumigatus protein reveals unique properties including N-linked glycosylation and a Ca2+-binding site whose occupancy regulates activity. The structure in complex with a substrate analogue suggests a catalytic mechanism that is distinct from those of known NADases, ADP-ribosyl cyclases and transferases. We propose that fungal NADases may convey advantages during interaction with the host or competing microorganisms.

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Snail mucus from the mantle and foot of two land snails, Lissachatina fulica and Hemiplecta distincta, exhibits different protein profile and biological activity

BMC Research Notes ◽

10.1186/s13104-021-05557-0 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Nattaphop Noothuan ◽

Kantamas Apitanyasai ◽

Somsak Panha ◽

Anchalee Tassanakajon

Keyword(s):

Biological Activities ◽

Protein Pattern ◽

Protein Profile ◽

Antibacterial Properties ◽

Biological Properties ◽

Specific Protein ◽

Land Snails ◽

Snail Species ◽

Significant Difference ◽

Different Types

Abstract Objective Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical and cosmetic applications. In this study, we explored the protein pattern and compared the biological properties of the mucus secreted from the mantle collar and foot of two snail species, Lissachatina fulica and Hemiplecta distincta. Result Protein profile showed a different pattern between the two species and between the two secretory parts. The mantle-specific protein bands were further characterized and among them was an antibacterial protein, achacin. Accordingly, the mucus from the mantle exhibited the higher antibacterial activity than that from the foot in both snail species. The mucus from H. distincta, first reported here, also showed antibacterial properties, but with a lower activity compared to that for L. fulica. Snail mucus also exhibited anti-tyrosinase activity and antioxidant activity but with no significant difference between the foot and mantle mucus. These results indicate some different protein compositions and biological activities of snail slime from the mantle and foot, which might be associated with their specific functions in the animal and are useful for medical applications.

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Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome

Diagnostics ◽

10.3390/diagnostics11030456 ◽

2021 ◽

Vol 11 (3) ◽

pp. 456

Author(s):

Lucia Santorelli ◽

William Morello ◽

Elisa Barigazzi ◽

Giulia Capitoli ◽

Chiara Tamburello ◽

...

Keyword(s):

Nephrotic Syndrome ◽

Idiopathic Nephrotic Syndrome ◽

Protein Profile ◽

Therapy Response ◽

Extracellular Vesicle ◽

Distinct Pattern ◽

Specific Patient ◽

Steroid Resistant ◽

Primary Glomerular Disease ◽

Disease Signature

Idiopathic nephrotic syndrome (INS) is the most frequent primary glomerular disease in children, displaying high grade proteinuria and oedema. The mainstay of therapy are steroids, and patients are usually classified according to the treatment response (sensitive vs. resistant). The mechanisms involved in INS pathogenesis and treatment responsiveness have not yet been identified. In this context, the analysis of urinary extracellular vesicles (UEv) is interesting, since they represent a molecular snapshot of the parental cells, offering a “fingerprint” for monitoring their status. Therefore, the aim of this study is to verify the feasibility of using UEv of INS patients as indicators of therapy response and its prediction. UEv were isolated from the urine of pediatric patients in remission after therapy; they showed characteristic electrophoresis profiles that matched specific patient subgroups. We then built a statistical model to interpret objectively each patient UEv protein profile: in particular, steroid-resistant patients cluster together with a very distinct pattern from other INS patients and controls. In conclusion, the evaluation of the UEv protein profile looks promising in the investigation of INS, showing a disease signature that might predict clinical evolution.

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