HrpNEa-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana

The complex interactions between aphids and their host plant are species-specific and involve multiple layers of recognition and defense. Aphid salivary proteins, which are released into the plant during phloem feeding, are a likely mediator of these interactions. In an approach to identify aphid effectors that facilitate feeding from host plants, eleven Myzus persicae (green peach aphid) salivary proteins and the GroEL protein of Buchnera aphidicola, a bacterial endosymbiont of this aphid species, were expressed transiently in Nicotiana tabacum (tobacco). Whereas two salivary proteins increased aphid reproduction, expression of three other aphid proteins and GroEL significantly decreased aphid reproduction on N. tabacum. These effects were recapitulated in stable transgenic Arabidopsis thaliana plants. Further experiments with A. thaliana expressing Mp55, a salivary protein that increased aphid reproduction, showed lower accumulation of 4-methoxyindol-3-ylmethylglucosinolate, callose and hydrogen peroxide in response to aphid feeding. Mp55-expressing plants also were more attractive for aphids in choice assays. Silencing Mp55 gene expression in M. persicae using RNA interference approaches reduced aphid reproduction on N. tabacum, A. thaliana, and N. benthamiana. Together, these results demonstrate a role for Mp55, a protein with as-yet-unknown molecular function, in the interaction of M. persicae with its host plants.

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Redox responses of Arabidopsis thaliana to the green peach aphid, Myzus persicae

Molecular Plant Pathology ◽

10.1111/mpp.13054 ◽

2021 ◽

Author(s):

Junhuan Xu ◽

Carmen S. Padilla ◽

Jiamei Li ◽

Janithri Wickramanayake ◽

Hillary D. Fischer ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Myzus Persicae ◽

Green Peach Aphid

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Fatty Acid Desaturases in the chloroplast and endoplasmic reticulum promote susceptibility to the Green Peach Aphid, Myzus persicae, in Arabidopsis thaliana

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-12-20-0345-r ◽

2021 ◽

Author(s):

Jiamei Li ◽

Aravind L. Galla ◽

Carlos Augusto Avila ◽

Kaitlin Flattmann ◽

Kaleb L. Vaughn ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Endoplasmic Reticulum ◽

Fatty Acid ◽

Myzus Persicae ◽

Acyl Carrier Protein ◽

Green Peach Aphid ◽

Single Species ◽

Aphid Resistance ◽

Fatty Acid Desaturases ◽

Triple Mutant

Fatty acid desaturases (FADs) in plants influence levels of susceptibility to multiple stresses, including insect infestations. In this study, infestations of the greAtFABen peach aphid (Myzus persicae) on Arabidopsis thaliana were reduced by mutations in three desaturases: FAB2/SSI2, which encodes a chloroplastic stearoyl-[acyl-carrier-protein] 9-desaturase, and AtFAD7 or AtFAD3, which encode ω-3 FADs in the chloroplast and endoplasmic reticulum (ER) respectively. These data indicate that certain FADs promote susceptibility to aphids, and that aphids are impacted by desaturases in both the chloroplast and ER. Aphid resistance in ssi2, fad3, and/or fad7 might involve altered signaling between these subcellular compartments. C18:1 levels are depleted in ssi2, whereas C18:2 accumulation is enhanced in fad3 and fad7. In contrast, fad8 has higher than normal C18:2 levels but also high C18:1 and low C18:0, and does not impact aphid numbers. Potentially, aphids may be influenced by the balance of multiple fatty acids (FAs) rather than by a single species, with C18:2 promoting aphid resistance and C18:1 promoting susceptibility. Although the fad7 mutant also accumulates higher-than-normal levels of C16:2, this FA does not contribute to aphid resistance because a triple mutant line that lacks detectable levels of C16:2 (fad2fad6fad7) retains comparable levels of aphid resistance as fad7. In addition, aphid numbers are unaffected by the fad5 mutation that inhibits C16:1 synthesis. Together, these results demonstrate that certain FADs are important susceptibility factors in plant-aphid interactions, and that aphid resistance is more strongly associated with differences in C18 abundance than C16 abundance.

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Antibiosis against the green peach aphid requires the Arabidopsis thaliana MYZUS PERSICAE-INDUCED LIPASE1 gene

The Plant Journal ◽

10.1111/j.1365-313x.2010.04378.x ◽

2010 ◽

Vol 64 (5) ◽

pp. 800-811 ◽

Cited By ~ 24

Author(s):

Joe Louis ◽

Katarzyna Lorenc-Kukula ◽

Vijay Singh ◽

John Reese ◽

Georg Jander ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Myzus Persicae ◽

Green Peach Aphid

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Nutrition Outweighs Defense: Myzus Persicae (Green Peach Aphid) Prefers and Performs Better on Young Leaves of Cabbage

10.1101/085159 ◽

2017 ◽

Author(s):

He-He Cao ◽

Zhan-Feng Zhanga ◽

Xiao-Feng Wang ◽

Tong-Xian Liu

Keyword(s):

Myzus Persicae ◽

Green Peach Aphid ◽

Sucrose Solution ◽

Molar Ratio ◽

Future Research ◽

Feeding Time ◽

Leaf Ontogeny ◽

Phloem Sap ◽

Young Leaves ◽

Phloem Feeding

AbstractPlant leaves of different ages differ in nutrition and toxic metabolites and thus exhibit various resistance levels against insect herbivores. However, little is known about the relationship between leaf ontogeny and plant resistance against phloem-feeding insects. In this study, we found that the green peach aphid, Myzus persicae Sulzer, preferred to settle on young cabbage leaves (Brassica oleracea L. var. capitata) rather than mature or old leaves, although young leaves contained the highest concentration of glucosinolates. Furthermore, aphids feeding on young leaves had higher levels of glucosinolates in their bodies, but aphids performed better on young leaves in terms of body weight and population growth. The concentration of glutamine in young leaves was the highest, which stimulated aphids feeding when added to the sucrose solution. Phloem sap of young leaves had higher amino acid:sucrose molar ratio than mature leaves, and aphids feeding on young leaves showed two times longer phloem feeding time and five times more dry honeydew excretion than on other leaves. These results indicate that aphids acquired the highest amount of nutrition and defensive metabolites when feeding on young cabbage leaves that are strong natural plant sinks. The higher phloem sap availability of young leaves likely contributes to the attractiveness and suitability for aphids and may compensate the negative effects of glucosinolates on aphids. According to these findings, we propose that phloem sap availability influenced by leaf ontogeny and source-sink status play a significant role in plant-aphid interaction, which desires more attention in future research.

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