Early detection of elevated lactate levels in a mitochondrial disease model using chemical exchange saturation transfer (CEST) and magnetic resonance spectroscopy (MRS) with 7T MR imaging

2019 ◽  
Vol 12 (2) ◽  
pp. 232-233
Author(s):  
Shigeyoshi Saito ◽  
Yusuke Takahashi ◽  
Akiko Ohki ◽  
Yasunori Shintani ◽  
Takahiro Higuchi
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Mr Imaging ◽  
Chemical Exchange ◽  
Disease Model ◽  
Chemical Exchange Saturation Transfer ◽  
Resonance Spectroscopy ◽  
Saturation Transfer ◽  
Lactate Levels ◽  
Elevated Lactate

scholarly journals pH Mapping of Skeletal Muscle by Chemical Exchange Saturation Transfer (CEST) Imaging

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2610
Author(s):  
Yu-Wen Chen ◽  
Hong-Qing Liu ◽  
Qi-Xuan Wu ◽  
Yu-Han Huang ◽  
Yu-Ying Tung ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Magnetic Resonance ◽  
Ph Value ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Resonance Spectroscopy ◽  
Saturation Transfer ◽  
Ph Changes ◽  
Cest Imaging

Magnetic resonance imaging (MRI) is extensively used in clinical and basic biomedical research. However, MRI detection of pH changes still poses a technical challenge. Chemical exchange saturation transfer (CEST) imaging is a possible solution to this problem. Using saturation transfer, alterations in the exchange rates between the solute and water protons because of small pH changes can be detected with greater sensitivity. In this study, we examined a fatigued skeletal muscle model in electrically stimulated mice. The measured CEST signal ratio was between 1.96 ppm and 2.6 ppm in the z-spectrum, and this was associated with pH values based on the ratio between the creatine (Cr) and the phosphocreatine (PCr). The CEST results demonstrated a significant contrast change at the electrical stimulation site. Moreover, the pH value was observed to decrease from 7.23 to 7.15 within 20 h after electrical stimulation. This pH decrease was verified by 31P magnetic resonance spectroscopy and behavioral tests, which showed a consistent variation over time.

scholarly journals Magnetic resonance spectroscopy of atypical diffuse pontine masses

2003 ◽  
Vol 15 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Mark D. Krieger ◽  
Stefan Blüml ◽  
J. Gordon McComb
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Mr Imaging ◽  
Resonance Spectroscopy ◽  
Prognostic Information ◽  
Original Diagnosis ◽  
Dismal Prognosis ◽  
Diffuse Pontine Glioma ◽  
Lactate Levels ◽  
Elevated Lipid

Object Diffuse pontine gliomas in children carry a dismal prognosis, with a mean survival of less than 1 year despite therapy. The diagnosis is based on the characteristic changes demonstrated on traditional magnetic resonance (MR) imaging. A few typically MR imaging–appearing pontine masses, however, do not behave in the expected fashion, which calls the original diagnosis into question. Methods The authors conducted a retrospective review of data obtained in 42 children (age 6 months–13 years) in whom diffuse pontine glioma had been diagnosed at their institution. Five of these patients (12%) survived longer than expected (> 8 months). There were no differences in these patients in terms of demographics, presentation, traditional imaging findings, or treatment compared with the group as a whole. Magnetic resonance spectroscopy, however, demonstrated two distinct patterns not seen in typical diffuse pontine gliomas. In two patients elevated lipid and lactate levels were shown, with decreased levels of choline, myoinositol, and n-acetyl-aspartate (NAA). In the other patients strikingly elevated choline/creatinine ratios and myoinositol levels were observed in comparison with typical pontine tumors. Conclusions These MR spectroscopy patterns demonstrated in this retrospective study seem to convey prognostic information and may lead to an expansion of this diagnostic tool.

scholarly journals Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo: A Biomarker for LDH Activity

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Catherine DeBrosse ◽  
Ravi Prakash Reddy Nanga ◽  
Puneet Bagga ◽  
Kavindra Nath ◽  
Mohammad Haris ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Magnetic Resonance ◽  
Musculoskeletal Diseases ◽  
Chemical Exchange ◽  
Radioactive Isotopes ◽  
Chemical Exchange Saturation Transfer ◽  
Resonance Spectroscopy ◽  
Multiple Quantum ◽  
Saturation Transfer ◽  
Wide Range

Abstract Non-invasive imaging of lactate is of enormous significance in cancer and metabolic disorders where glycolysis dominates. Here, for the first time, we describe a chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method (LATEST), based on the exchange between lactate hydroxyl proton and bulk water protons to image lactate with high spatial resolution. We demonstrate the feasibility of imaging lactate with LATEST in lactate phantoms under physiological conditions, in a mouse model of lymphoma tumors and in skeletal muscle of healthy human subjects pre- and post-exercise. The method is validated by measuring LATEST changes in lymphoma tumors pre- and post-infusion of pyruvate and correlating them with lactate determined from multiple quantum filtered proton magnetic resonance spectroscopy (SEL-MQC 1H-MRS). Similarly, dynamic LATEST changes in exercising human skeletal muscle are correlated with lactate determined from SEL-MQC 1H-MRS. The LATEST method does not involve injection of radioactive isotopes or labeled metabolites. It has over two orders of magnitude higher sensitivity compared to conventional 1H-MRS. It is anticipated that this technique will have a wide range of applications including diagnosis and evaluation of therapeutic response of cancer, diabetes, cardiac and musculoskeletal diseases. The advantages of LATEST over existing methods and its potential challenges are discussed.

scholarly journals Chemical Exchange Saturation Transfer (CEST) MR Imaging for the Diagnosis of Brain Tumors

2020 ◽  
Author(s):  
Sachi OKUCHI ◽  
Yasutaka FUSHIMI ◽  
Tomohisa OKADA ◽  
Satoshi NAKAJIMA ◽  
Akihiko SAKATA ◽  
...  
Keyword(s):  
Brain Tumors ◽  
Mr Imaging ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer
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