GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds

Sheep in Bangladesh are well known as efficient users of low quality roughages, welladapted to hot and humid agro-climatic conditions, capable of bi-annual lambing withmultiple births and resistant to common prevalent diseases. They play an important role inthe supply of animal protein. Present study aimed to investigate possible association ofGDF9 gene polymorphisms with litter size. A total of 126 DNA samples extracted from theblood of indigenous sheep from 5 locations namely Tangail, Noakhali, Naogaon,Gaibandha and Satkhira with known litter size were used to study the association ofGDF9 polymorphism by PCR based RFLP method. Two polymorphic regions of GDF9(FecG1 and FecG8) were amplified by PCR, digested with respective restriction enzymesand 126 sheep were genotyped. Current study revealed that genotype and allelefrequency for FecG1 varied among the sheep from different locations. The genotype (GG,AG, AA) frequency were 51.59%, 45.24% and 3.17% and the allele (G and A) frequenciesin the overall population were 74.21% and 25.79%, respectively. There was a significantassociation of FecG1 of GDF9 gene polymorphism with litter size. The homozygous GGgenotype had the lowest litter size (1.59±0.09; n=65) and homozygous AA genotype hadthe highest litter size (2.00±0.41; n=4). No genotypic variations were found for FecG8.Findings of this study specially the polymorphism of FecG1 together with genotyping ofsome sheep could be utilized in the selection program to increase the lamb productionpotentiality of indigenous sheep of Bangladesh. Res. Agric., Livest. Fish.7(2): 283-292, August 2020

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Genetic polymorphism in fecundity gene (FecG) among Indiansheep breeds Balangir, Shahabadi and Bonpala

Indian Journal of Animal Research ◽

10.18805/ijar.9648 ◽

2016 ◽

Author(s):

Jowel Debnath ◽

Ran Vir Singh

Keyword(s):

Litter Size ◽

Transforming Growth Factor ◽

Natural Habitat ◽

Factor B ◽

Sheep Breeds ◽

Homozygous Genotype ◽

Average Litter Size ◽

Pcr Rflp ◽

Selection Of ◽

Gdf9 Gene

FecG (GDF9) is a member of the transforming growth factor-b (TGF- b) superfamily, have been shown to be essential for follicular growth and ovulation. Different mutations in FecG gene caused increased ovulation and infertility in sheep. The present study was designed for screening polymorphism of FecG gene in 250 selected ewes from different sheep flocks representing Balangir (100), Shahabadi (100) and Bonpala (50) by employing forced PCR-RFLP technique. Genomic DNA was extracted from the blood of Balangir, Shahabadi and Bonpala matured ewes with average litter size varying from 1.00± 0.00 to 1.14±0.02 at different parities. Digestion of FecG (GDF9) gene with DdeI restriction enzyme resulted into FecGHH homozygous genotype. In all three sheep breeds, genotypic frequencies of FecGHH were 100% and gene frequency of H allele was unity. This indicates that the FecG gene is fixed in the Balangir, Shahabadi and Bonpala population in the natural habitat. Litter size of Balangir and Bonpala sheep breeds were single but in Shahabadi sheep twin was recorded. In present study all the animals of three breeds were homozygous for FecG and there was no infertility observed in above mentioned breeds in field condition and organized farm, which is not in consonance with previous report. The observed effects could be caused by linkage disequilibrium with other nearby loci. The study revealed that FecG gene is not a reliable genetic marker for selection of high prolificacy in sheep.

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Variant <i>GDF9</i> mRNA is likely not the main cause of larger litter size in Iranian Lori-Bakhtyari, Shal, Ghezel, and Afshari sheep breeds

Archives Animal Breeding ◽

10.5194/aab-60-119-2017 ◽

2017 ◽

Vol 60 (2) ◽

pp. 119-129 ◽

Cited By ~ 3

Author(s):

Shahin Eghbalsaied ◽

Farzad Rashidi Khorasgani ◽

Hamid-Reza Amini ◽

Majid Farahi ◽

Maryam Davari ◽

...

Keyword(s):

Litter Size ◽

Signal Peptide ◽

Large Dataset ◽

Sheep Breeds ◽

Signal Peptide Region ◽

Twin Birth ◽

Significant Difference ◽

Pcr Rflp ◽

Peptide Region ◽

Gdf9 Gene

Abstract. This study was carried out to screen the GDF9 gene and evaluate the polymorphism effect on litter size of four Iranian sheep breeds using the PCR-RFLP and PCR-SSCP methods. First, sequencing of the GDF9 gene in 16 twin-birth, 4 triplet-birth, and 2 infertile ewes showed that, in addition to G2, G3, G4, G5, and G6 mutations that have been previously reported in other breeds, a new G0 mutation, called C25T, exists in the GDF9 sequence of 1 out of 22 ewes and causes L9F substitution in the signal peptide region. None of the triplet-birth or infertile ewes carried G1, G4, G7, FecGE, G8, or FecGT mutations. In the second experiment, a large dataset was used: 605 individuals including 496 ewes (145 Afshari, 54 Shal, 126 Ghezel, and 171 Lori-Bakhtyari sheep), and 109 rams (26 Afshari, 23 Shal, 10 Ghezel, and 50 Lori-Bakhtyari sheep. There were no sheep carrying the G7, G8, or Thoka mutations. Among all 109 rams that were used in this study, none of them were homozygous for the G1 mutation. Moreover, abundance of heterozygote rams (G1/G+) varied from 0.0 (Afshari) to 28.6 % (Lori-Bakhtyari and Ghezel). The highest and the lowest frequencies of the G4 mutation were 30.6 and 3.0 % in Shal and Afshari breeds, respectively. Moreover, G4 abundance varied from 0.0 to 42.3 %, from 3.0 to 26.9, and from 3.0 to 30.6 % in rams, ewes, and overall, respectively. There was a significant difference in the abundance of G1 and G4 mutations between breeds. However, neither the G1 nor the G4 mutation was associated with litter size in Afshari, Ghezel, Lori-Bakhtyari, or Shal sheep breeds. In conclusion, the results of this study showed that GDF9 G1 and G4 mutations are not the reason for higher litter size in Iranian sheep. Moreover, the GDF9 G0 and G6 mutations do not cause triplet births or infertility in Iranian ewes. Therefore, it is unlikely that variant GDF9 mRNA induces larger litter size or infertility in Iranian ewes.

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G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

Veterinary World ◽

10.14202/vetworld.2021.104-112 ◽

2021 ◽

Vol 14 (1) ◽

pp. 104-112

Author(s):

Amani Z. Abdelgadir ◽

Lutfi M. A. Musa ◽

Khaleel I. Jawasreh ◽

Aubai. O. Saleem ◽

Faisal El-Hag ◽

...

Keyword(s):

Point Mutation ◽

Litter Size ◽

Reference Sequence ◽

Wild Type ◽

Mutant Type ◽

Type Allele ◽

Sheep Breeds ◽

Differentiation Factor ◽

Growth Differentiation Factor 9 ◽

Gdf9 Gene

Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

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Polymorphism Detection of GDF9 Gene and Its Association with Litter Size in Luzhong Mutton Sheep (Ovis aries)

Animals ◽

10.3390/ani11020571 ◽

2021 ◽

Vol 11 (2) ◽

pp. 571

Author(s):

Fengyan Wang ◽

Mingxing Chu ◽

Linxiang Pan ◽

Xiangyu Wang ◽

Xiaoyun He ◽

...

Keyword(s):

Litter Size ◽

Tertiary Structure ◽

Novel Mutation ◽

Major Gene ◽

Ovis Aries ◽

Nucleotide Polymorphisms ◽

Economic Traits ◽

Coding Region ◽

Association Analyses ◽

Gdf9 Gene

Litter size is one of the most important economic traits in sheep. GDF9 and BMPR1B are major genes affecting the litter size of sheep. In this study, the whole coding region of GDF9 was sequenced and all the SNPs (single nucleotide polymorphisms) were determined in Luzhong mutton ewes. The FecB mutation was genotyped using the Sequenom MassARRAY®SNP assay technology. Then, the association analyses between polymorphic loci of GDF9 gene, FecB, and litter size were performed using a general linear model procedure. The results showed that eight SNPs were detected in GDF9 of Luzhong mutton sheep, including one novel mutation (g.41769606 T > G). The g.41768501A > G, g.41768485 G > A in GDF9 and FecB were significantly associated with litter size in Luzhong mutton ewes. The g.41768485 G > A is a missense mutation in the mature GDF9 protein region and is predicted to affect the tertiary structure of the protein. The results preliminarily demonstrated that GDF9 was a major gene affecting the fecundity of Luzhong mutton sheep and the two loci g.41768501A > G and g.41768485 G > A may be potential genetic markers for improving litter size.

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CAST/MspI gene polymorphism and its impact on growth traits of Soviet Merinoand Salsk sheep breeds in the South European part of Russia

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES ◽

10.3906/vet-1507-101 ◽

2016 ◽

Vol 40 ◽

pp. 399-405 ◽

Cited By ~ 3

Author(s):

Ivan FedIvan GORLOV ◽

Nadezhda Vasilevna SHIROKOVA ◽

Alexander Vasilevich RANDELIN ◽

Valeriya Nikolaevna VORONKOVA ◽

Natalya Ivanovna MOSOLOVA ◽

...

Keyword(s):

Gene Polymorphism ◽

Growth Traits ◽

European Part ◽

The South ◽

Sheep Breeds ◽

European Part Of Russia

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THE Β-LG GENE POLYMORPHISM LEVEL OF ASCANIAN KARAKUL AND ASCANIAN FINE-FLEECED SHEEP BREEDS

International Scientific Conference ◽

10.30525/978-9934-26-002-5-45 ◽

2020 ◽

Author(s):

Kostiantyn Skrepets ◽

◽

Hanna Yakovchuk ◽

Keyword(s):

Gene Polymorphism ◽

Sheep Breeds ◽

Polymorphism Level

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Repeatability of ovulation rate and its relationship with litter size in four sheep breeds

Genetics Selection Evolution ◽

10.1186/1297-9686-9-1-126c ◽

1977 ◽

Vol 9 (1) ◽

pp. 126c

Author(s):

JP Hanrahan

Keyword(s):

Litter Size ◽

Ovulation Rate ◽

Sheep Breeds

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Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

Animals ◽

10.3390/ani9110958 ◽

2019 ◽

Vol 9 (11) ◽

pp. 958 ◽

Cited By ~ 3

Author(s):

Yuan ◽

Zhang ◽

Li ◽

Wang ◽

Li ◽

...

Keyword(s):

Candidate Genes ◽

Genetic Markers ◽

Litter Size ◽

Nucleotide Polymorphisms ◽

Female Reproduction ◽

Dna Pooling ◽

High Fecundity ◽

Sheep Breeds ◽

Hu Sheep ◽

Six Genes

Hu sheep and Small-tailed Han sheep are the most widely raised and most famous maternal sheep breeds in China, which are known for precocious puberty, perennial oestrus and high fecundity (1–6 lambs each parity). Therefore, it is crucial to increase litter size of these two breeds for intensive sheep industry. The objective of this study was to identify potential genetic markers linked with sheep litter size located at ten genes. This study collected blood sample of 537 Hu sheep and 420 Small-tailed Han sheep with litter size of first parity. The average litter sizes in Hu sheep and Small-tailed Han sheep were 2.21 and 1.93. DNA-pooling sequencing method was used for detecting the potential single nucleotide polymorphisms (SNPs) in ten genes related to follicle development and female reproduction. SNPscan® was used for individually genotyping. As a result, a total of 78 putative SNPs in nine out of ten candidate genes (except NOG) were identified. In total, 50 SNPs were successfully genotyped in Hu sheep and Small-tailed Han sheep. After quality control, a total of 42 SNPs in Hu sheep and 44 SNPs in Small-tailed Han sheep were finally used for further analysis. Association analysis revealed that nine SNPs within six genes (KIT: g.70199073A>G, KITLG: g.124520653G>C, ADAMTS1: g.127753565T>C, ADAMTS1: g.127754640G>T, NCOA1: g.31928165C>T, NCOA1: g.32140565G>A, LIFR: g.35862868C>T, LIFR: g.35862947G>T and NGF: g.91795933T>C) were significantly associated with litter size in Hu sheep or Small-tailed Han sheep. A combined haplotypes analysis of the two loci (LIFR: g.35862868C>T and LIFR: g.35862947G>T) revealed that H2H3 (CTTT) combined haplotypes had the largest litter size than the rest combined haplotypes and more than those with either mutation alone in Small-tailed Han sheep. Taken together, our study suggests that nine significant SNPs in six genes can be served as useful genetic markers for MAS in sheep.

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