Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources

2019 ◽  
Vol 96 (4) ◽  
pp. 396-402 ◽  
Author(s):  
Lili Jiang ◽  
Ziquan Wang ◽  
Guanghui Jin ◽  
Dianqiu Lu ◽  
Xuezhan Li
2018 ◽  
Vol 42 (5) ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Gonzalo Sanchez Correa ◽  
Berta Elizabet Llorente

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Kho Ying Han ◽  
Bee Lynn Chew ◽  
Sreeramanan Subramaniam

AbstractThe use of artificial light sources such as light-emitting diodes (LEDs) has become a prerequisite in tissue culture studies to obtain morphogenetic enhancements on in vitro plants. This technology is essential for developmental enhancements in the growing plant cultures due to its light quality and intensity greatly influencing the in vitro growing explants at a cellular level. The current study investigates the effects of different light-emitting diode (LED) spectra on the growth of apical buds of Ficus carica var. Black Jack. Ficus carica, commonly known as figs is rich in vitamins, minerals, and phytochemicals capable of treating microbial infections and gastric, inflammatory, and cardiac disorders. Apical buds of Ficus carica var. Black Jack, presented morphogenetic changes when grown under six different LED spectra. The highest multiple shoots (1.80 per growing explant) and healthy growing cultures were observed under the blue + red LED spectrum. Wound-induced callus formation was observed on apical buds grown under green LED spectrum and discolouration of the growing shoots were observed on the cultures grown under far-red LED spectrum. Multiple shoots obtained from the blue + red LED treatment were rooted using 8 µM indole-3-acetic acid (IAA), and the rooted plantlets were successfully acclimatised. Compared with the other monochromatic LEDs, blue + red proved to be significantly better for producing excellent plant morphogeny. It is apparent that blue and red LED is the most suitable spectra for the healthy development of plants. The findings have confirmed that the combination of blue + red LED can potentially be used for enhancing growth yields of medicinally and commercially important plants.


2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Jinren Yan

A reflector-based light-emitting diode (LED) luminaire structure that can achieve a large cut-off angle for general lighting is presented in this work. The proposed lighting unit mainly consists of a spherical reflector and a primary packaging lens that contains an aspheric surface and a spherical surface. The light rays emitted from the LED light source are well controlled by the spherical reflector and the aspheric surface of the lens for the purpose of obtaining a uniform illumination on the target surface. Both the ideal Lambertian LED and non-Lambertian LED light sources were employed to validate the proposed structure and the performance of the designed lighting units was analyzed by optical simulation. The results show that the light utilization efficiencies and the estimated uniformities are 92.96% and 91.11% for ideal Lambertian LED-based lighting unit and 93.31% and 91.64% for non-Lambertian LED-based lighting unit, respectively. Further analysis shows that the tolerances of horizontal, vertical, and rotational deviation of the both lighting units were about 2.0 mm, 1.0 mm, and 1.0°, respectively.


2007 ◽  
Vol 8 (2) ◽  
pp. 43-51 ◽  
Author(s):  
Barry M. Owens ◽  
Kelbin H. Rodriguez

Abstract Aims Light-emitting diode (LED) polymerization of dental restorative materials has become increasingly popular. However, individual light-curing unit (LCU) functions (intensity and/or wavelength emission) may not conform to manufacturer specifications due to quality control issues. The purpose of this study was to evaluate the quality of irradiance, in terms of power density (intensity) and spectral distribution (peak wavelength), emitted from LED and quartz-tungsten halogen (QTH) LCUs in vitro. The battery expenditure of these LED units was also tested. Methods and Materials The intensity and spectral distribution from four third generation LED (Smartlite PS, Coltolux LED, radii Plus, Diopower) and one QTH (Schein Visible Cure) light sources were measured using six different dental curing light meters (Coltolux, Cure Right, Demetron 100, Demetron LED., Hilux, and Light Meter-200) and a visible-ultraviolet light spectrophotometer (Hitachi Elmer-Perkins). The battery life was also plotted for each light source following a 1500 second duration period. The data obtained from radiometric and spectrophotometric analysis was compared to manufacturer specifications. Results Radiometric evaluation revealed LED light units tested did not satisfy manufacturer claims for minimum intensities. Spectral emissions from the LED light sources did meet manufacturer requirements. No clinically appreciable battery drain was evidenced from testing all re-chargeable LED units. Conclusion Despite limitations LED technology appears to be an effective alternative for curing of lightactivated esthetic restorative materials. Additional advantages associated with LED curing lights include ergonomic handling capabilities, negative heat generation, and minimal maintenance concerns. Citation Owens BM, Rodriguez KH. Radiometric and Spectrophotometric Analysis of Third Generation Light- Emitting Diode (LED) Light-Curing Units. J Contemp Dent Pract 2007 February;(8)2:043-051.


2011 ◽  
Vol 04 (01) ◽  
pp. 45-52 ◽  
Author(s):  
GUANGDA LIU ◽  
CHANGE PAN ◽  
KAI LI ◽  
YUAN TAN ◽  
XUNBIN WEI

In this paper, we studied portable blue and red light-emitting-diode (LED) light sources in phototherapy for mild to moderate acne vulgaris to evaluate the efficacy and tolerance of patients. Patients, randomly divided into blue and red groups, received either blue or red LED phototherapy twice a week for four weeks. After complete treatment, the number of lesions reduced by 71.4% in the blue group, in contrast to 19.5% in the red group. No obvious side effects were observed during and one month after the treatment, except for some mild dryness mentioned by several patients.


2021 ◽  
Author(s):  
Olga V. Nakonechnaya ◽  
◽  
Evgeniy P. Subbotin ◽  
Olga V. Grishchenko ◽  
Irina V. Gafitskaya ◽  
...  

We studied growth of potato plantlets of two cultivars under different constant polychromatic light-emitting diodes (LEDs) and dynamic lighting with the spectrum modifications during growth. Light sources constructed by us were sunbox SB, red-green-blue RGB, and full spectrum FS, red-blue. White luminescent lamps were used as control light. Dynamic lighting was achieved by transferring groups of plantlets from one box to another. Red/green/blue portions in the light sources were (in percents): 39R/39G/22B for SB, 63R/21G/16B for RGB, 74R/8G/18B for FS, and 22R/49G/29B for control. PPFD was set at 45 μmol/m2 s. Morphometric measurements were made 14 and 28 days after planting. Plantlets developed differently from initial stages. Cultivar 'Red Scarlett' appeared to be more susceptible to different lighting than 'Innovator'. Light variations RGB and RGB-SB were the most favorable for plantlet development. This is the first report on the dynamic lighting application for growing potato plantlets.


2021 ◽  
Author(s):  
Wendong Nie ◽  
Liqin Yao ◽  
Guang Chen ◽  
Shihao Wu ◽  
Zhijian Liao ◽  
...  

The near-infrared (NIR) phosphor-converted light emitting diode (pc-LED) light sources have broad application prospects in environmental science, biomedical and plant growth fields. However, NIR phosphor still suffers from narrowband emission...


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