Cooling Characteristic of a Wall Jet for Suppressing Crossflow Effect under Conjugate Heat Transfer Condition

The leading edge is the critical portion for a gas turbine blade and is often insufficiently cooled due to the adverse effect of Crossflow in the cooling chamber. A novel internal cooling structure, wall jet cooling, can suppress Crossflow effect by changing the coolant flow direction. In this paper, the conjugate heat transfer and aerodynamic characteristics of blades with three different internal cooling structures, including impingement with a single row of jets, swirl cooling, and wall jet cooling, are investigated through RANS simulations. The results show that wall jet cooling combines the advantages of impingement cooling and swirl cooling, and has a 19–54% higher laterally-averaged overall cooling effectiveness than the conventional methods at different positions on the suction side. In the blade with wall jet cooling, the spent coolant at the leading edge is extracted away through the downstream channels so that the jet could accurately impinge the target surface without unnecessary mixing, and the high turbulence generated by the separation vortex enhances the heat transfer intensity. The Coriolis force induces the coolant air to adhere to the pressure side’s inner wall surface, preventing the jet from leaving the target surface. The parallel cooling channels eliminate the common Crossflow effect and make the flow distribution of the orifices more uniform. The trailing edge outlet reduces the entire cooling structure’s pressure to a low level, which means less penalty on power output and engine efficiency.

Surface Texturing Technique Based on Ultrasonic Turning for Improving Tribological Properties

Tribological properties such as lubrication, friction, and wear resistance greatly affect machine operation efficiency, performance, and service life. Surface texturing methods such as scraping can be used to improve these properties. Scraping creates many small depressions on the target surface. These depressions, which are evenly distributed, function as oil holes and thus improve lubrication performance. This paper describes a surface texturing technique based on ultrasonic vibration-assisted turning (UVAT) that simultaneously improves tribological properties and machinability. In UVAT, the cutting tool is oscillated mainly in the principal direction. Vibration in the radial direction, which is induced by Poisson deformation, periodically digs up or pushes the workpiece surface in the radial direction, creating a textured surface. A surface subjected to UVAT has periodic depressions along the workpiece rotation direction. The texturing rate of UVAT is up to 6700 mm2/min, which is higher than that of manual scraping. To evaluate the tribological performance of a surface textured by UVAT, the friction coefficient between a stainless steel pin and the surface was measured under oil dipping conditions. The results of friction experiments show that the friction coefficient of the UVAT-treated surface and its fluctuation were lower than those of a conventional turned surface. The UVAT-treated surface had stable friction properties.

Influence of the Distance between Focusing Lens and Target Surface on the Characteristics of Laser-excited Lead Plasma

The present work investigated the effect of distance from target surface on the parameters of lead plasma excited by 1064nm Q-switched Nd:YAG laser. The excitation was conducted in air, at atmospheric pressure, with pulse length of 5 ns, and at different pulse laser energies. Electron temperature was calculated by Boltzmann plot method based on the PbI emission spectral lines (369.03 nm, 416.98 nm, 523.48, and 561.94 nm). The PbI lines were recorded at different distances from the target surface at laser pulse energies of 260 and 280 mJ. The emission intensity of plasma increased with increasing the lens-to-target distance. The results also detected an increase in electron temperature with increasing the distance between the focal lens and the surface of the target in all laser energies under study. In addition, the electron number density was determined by using the Stark broadening method. The data illustrated that the electron number density was increased with increasing the distance from target surface, reaching the maximum at a distance of 11 cm for all pulse laser energy levels under study.

Mortality of Fruit Flies (Diptera: Tephritidae) Exposed to Spinetoram Toxic Bait in the Laboratory

Fruit flies (Diptera: Tephritidae) cause significant losses during the production and marketing of horticultural products. Brazilian growers usually adopt full-coverage insecticide spraying to control fruit flies, but toxic bait is a more strategic technique, because reach efficacy and the target surface is the foliage and branches. We provide information regarding the toxicity of spinetoram bait to two fruit fly species in the laboratory as an alternative to organophosphates and the specific spinosad formulation. We tested toxic baits in the laboratory, using commercial hydrolysed corn protein (10% v/v) plus 90 g, 120 g, 150 g and 180 g dilutions of spinetoram 250 WG (commercial product/1,000 litres of water). All toxic baits were compared with an untreated control (only protein) for the adults of females and males of Anastrepha obliqua (Macquart, 1835) and Ceratitis capitata (Wiedemann, 1824) up to 30 hours of exposure. Dry food for adults was included in all dilutions (5% w/v). In addition, we tested the residual effect of toxic baits applied to the leaves of mandarin seedlings. We used the same treatments of the earlier bioassay without dry food, collecting treated leaves and exposing them to C. capitata (medfly) females for 24 hours in the laboratory. Leaves were collected 1, 3, 7, 15 and 30 days after application. Overall, medfly adults were more susceptible to spinetoram baits than A. obliqua. All toxic baits resulted in 100% C. capitata mortality 24 hours after initial exposure, and the toxic bait at 150 g/1,000 L of water resulted in the maximum mortality (96%) in A. obliqua. Except for 90 g of spinetoram bait at 30 days after application, all spinetoram bait concentrations resulted in significantly, more dead C. capitata females than the control over all tested periods in the residual bioassay. At 30 days after application, spinetoram baits at 120 g, 150 g and 180 g resulted in 85%, 87% and 86% mortality in C. capitata, respectively. Spinetoram toxic baits have proven promising for long-term fruit fly management.

SURFACE HEATER FABRICATION USING MICRO-LITHOGRAPHY FOR TRANSPIRATION COOLING HEAT TRANSFER COEFFICIENT MEASUREMENTS

Transpiration cooling is able to provide more uniform coolant coverage than film cooling to effectively protect the component surface from contacting the hot gas. Due to numerous coolant ejection outlets within a small area at the target surface, the experimental thermo-fluid investigation on transpiration cooing becomes a significant challenge. Two classic methods to investigate film cooling, the steady-state foil heater method and the transient thermography technique, both fail for transpiration cooling because the foil heater would block numerous coolant outlets, and the semi-infinite solid conduction model no longer holds for porous plates. In this study, a micro-lithography method to fabricate a silver coil pattern on top of the additively manufactured polymer porous media as the surface heater was proposed. The circuit was deliberately designed to cover the solid surface in a combination of series connection and parallel connection to ensure the power in each unit cell area at the target surface was identical. With uniform heat flux generation, the steady-state tests were conducted to obtain distributions of a pair of parameters, adiabatic cooling effectiveness, and heat transfer coefficient (HTC). The results showed that the adiabatic cooling effectiveness could reach 0.65 with a blowing ratio lower than 0.5. Meanwhile, the heat transfer coefficient ratio (hf/h0) of transpiration cooling was close to 1 with a small blowing ratio at 0.125. A higher HTC ratio was observed for smaller pitch-to-diameter cases due to more turbulence intensity generated at the target surface.

Active species evolution in presence of different targets impacted by helium plasma jet at atmospheric pressure

A low-temperature plasma jet is generated by a dielectric barrier discharge poweredby a pulsed high voltage in helium flow (3 L/min) at atmospheric pressure inpresence of different targets (glass slide or ultra-pure water or a grounded metal plate)positioned perpendicular to the plasma propagation axis. Experimental electricalcharacterizations as discharge current, voltage and powerand optical ones as intensifiedcamera ICCD, Schlieren imaging and emission spectroscopy to follow specific excitedspecies have been achieved. The transition from laminar to turbulent regime wereobserved during the discharge ignition with a larger spreading of the plasma on thesurface target with lower dielectric permittivity and the generation of two dischargesduring each voltage pulse is highlighted during the propagation of the ionization wavethat has shown a variable speed along the plasma axis not depending on the target kind.The evolution of some active species (as OH, O and excited nitrogen and helium) areinvestigated using time resolved mapping of the emissions of radiative excited speciespropagating in ambient air between the plasma jet output andthe target. For a lowrelative permittivity target (glass), the volume ionization wave at its arrival on thetarget spreads on its surface thus behaving as a surface ionization wave. For thehighest relative permittivity (metal), a conductive channel appears between the targetsurface and the plasma jet during the first discharge, followed by a diffuse plasma plumefrom the target surface towards the plasma jet after the impact of the ionization waveon the target. A hybrid behavior is highlighted for the ultra-pure water which leadsto a short spreading of the ionization wave on the target surface, the formation of aconductive channel in ambient air between tube output and target and the formationof a plasma plume on the target surface.

Generation of elastic geodesic gridshells with anisotropic cross sections

Geodesic gridshells are shell structures made of continuous elements following geodesic lines. Their properties ease the use of beams with anisotropic cross-sections by avoiding bending about their strong axis. However, such bending may arise when flattening arbitrary geodesic grids, which forbids their initial assembly on the ground. This study provides a process to design elastic geodesic gridshells, that is, gridshells that minimise bending moments in both formed and near-flat configurations. The generation process first brings a target geodesic network onto a plane by maintaining arc lengths. The flat mesh is then relaxed to minimise its main curvatures and hence bending moments in its members. The result is an elastic geodesic gridshell that can be assembled flat on the ground and then lifted up into its target surface. The method is applied to the design of six geodesic gridshells made of reclaimed skis.

Non-stationary convective heat transfer in an air synthetic impinging jet. Experiment and numerical simulation

An unsteady local heat transfer in an air synthetic non-steady-state jet impingement onto a flat plate with a variation of the Reynolds number, nozzle-to-plate distance and pulses frequency is experimentally and numerically studied. Measurements of the averaged and pulsating heat transfer at the stagnation point are conducted using a heat flux sensor. The axisymmetric URANS method and the Reynolds stress model are used for numerical simulations. For local values of heat transfer, zones with the maximum instantaneous value of heat flux and heat transfer coefficient are identified. The heat transfer increases at relatively low nozzle-to-plate distances (H/d ≤ 4). The heat transfer decreases at high distance from the orifice and target surface. An increase in the Reynolds number causes reduction of heat transfer.

Sensitive Immunofluorescent Detection of the PRAME Antigen Using a Practical Antibody Conjugation Approach

Bioconjugation of antibodies with various payloads has diverse applications across various fields, including drug delivery and targeted imaging techniques. Fluorescent immunoconjugates provide a promising tool for cancer diagnostics due to their high brightness, specificity, stability and target affinity. Fluorescent antibodies are widely used in flow cytometry for fast and sensitive identification and collection of cells expressing the target surface antigen. Nonetheless, current approaches to fluorescent labeling of antibodies most often use random modification, along with a few rather sophisticated site-specific techniques. The aim of our work was to develop a procedure for fluorescent labeling of immunoglobulin G via periodate oxidation of antibody glycans, followed by oxime ligation with fluorescent oxyamines. Here, we report a novel technique based on an in situ oxime ligation of ethoxyethylidene-protected aminooxy compounds with oxidized antibody glycans. The approach is suitable for easy modification of any immunoglobulin G, while ensuring that antigen-binding domains remain intact, thus revealing various possibilities for fluorescent probe design. The technique was used to label an antibody to PRAME, a cancer-testis protein overexpressed in a number of cancers. A 6H8 monoclonal antibody to the PRAME protein was directly modified with protected-oxyamine derivatives of fluorescein-type dyes (FAM, Alexa488, BDP-FL); the stoichiometry of the resulting conjugates was characterized spectroscopically. The immunofluorescent conjugates obtained were applied to the analysis of bone marrow samples from patients with oncohematological diseases and demonstrated high efficiency in flow cytometry quantification. The approach can be applied for the development of various immunofluorescent probes for detection of diagnostic and prognostic markers, which can be useful in anticancer therapy.