Erythrocyte Indices and Hemoglobin Analysis for α-Thalassemia Screening in an Area with High Carrying Rate

Abstract Background The quantification of hemoglobin A2 (Hb A2; α2δ2) is used as a valuable test to differentiate α- and ß-thal carriers in clinical laboratories. Therefore, the HBD (δ-globin) gene variants could result in reduced levels of Hb A2 and have implications for thalassemia screening programs. The aim of the present study was to predict the consequences of HBD gene variants identified in the Iranome project. Results The highest number of variants was in the Persian Gulf Islanders. The variants of p.Gln132Glu (HBD: c.394C>G), p.Gly17Arg (HBD: c.49G>C), p.Thr5Ile (HBD: c.14C>T), and p.Ala28Ser (HBD: c.82G>T) presented damage results in three or more prediction tools. In addition, it seems that the p.Gly30= (HBD: c.90C>T) decreases the use of authentic splice and, instead, creates a new donor splice site (DSS) or leads to the use of a cryptic DSS. Conclusions Most of these variants have been associated with a decrease in Hb A2 levels. Due to the high mutational diversity in the HBB gene in the Iranian population and the use of Hb A2 quantification to differentiate α- and ß-thal carriers among Iranian clinical laboratories, some attention should be taken to a possible co-inheritance of HBD gene variants to avoid the misdiagnosis of ß-thal carriers.

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Evaluation of four different HPLC devices for hemoglobinopathy screening

Turkish Journal of Biochemistry ◽

10.1515/tjb-2019-0484 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Müjgan Ercan Karadağ ◽

Emiş Deniz Akbulut ◽

Esin Avcı ◽

Esra Fırat Oğuz ◽

Saadet Kader ◽

...

Keyword(s):

Gold Standard ◽

High Performance ◽

Public Health Problem ◽

Reference Value ◽

Comparison Study ◽

Gold Standard Method ◽

Blood Samples ◽

Systematic Biases ◽

Hemoglobin Variants ◽

Thalassemia Screening

AbstractObjectiveHemoglobinopathies are a common public health problem in Turkey. In the screening of these disorders in population, cation-exchange high performance liquid chromatography (HPLC) is accepted as the gold standard method. In this study, the aim was to assess four different HPLC devices used in hemoglobinopathy screening.Materials and methodsA total of 58 blood samples were analyzed with four different HPLC methods (Bio-Rad variant II, Agilent 1100, Tosoh G8 and Trinity Ultra2 trademarks).ResultsThe comparison study demonstrated a good correlation between the results of each HPLC analyzer and the reference value obtained by averaging all the HbA2 results belonging to the methods tested in the study [ (Tosoh G8 (r=0.988), Bio-Rad variant II (r=0.993), Agilent 1100 (r=0.98) and Trinity Ultra2 (r=0.992) ]. HbA2 determination in the presence of HbE was interfered in both Bio-Rad variant II and Tosoh G8.ConclusionThe analyzers were found to have compatible HbA2 results but with accompanying different degrees of proportional and systematic biases. HPLC analyzers may be affected by different hemoglobin variants at different HbA2 concentrations, which is an important point to take into consideration during the evaluation of HbA2 results in thalassemia screening.

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Hemoglobin is more than a surrogate of tumour oxygenation: part 1. Relation of erythrocyte indices and body iron markers to oral cancer growth

European Journal of Cancer ◽

10.1016/s0959-8049(99)81150-2 ◽

1999 ◽

Vol 35 ◽

pp. S188

Author(s):

V.N. Bhattathiri

Keyword(s):

Oral Cancer ◽

Cancer Growth ◽

Erythrocyte Indices ◽

Body Iron ◽

Tumour Oxygenation

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Erythrocyte indices and serum ferritin in newborns

Revista Brasileira de Hematologia e Hemoterapia ◽

10.1590/s1516-84842010000500008 ◽

2010 ◽

Vol 32 (5) ◽

pp. 365-370 ◽

Cited By ~ 3

Author(s):

Maria de Fatima Pussick Nunes ◽

Ana Marlúcia Oliveira Assis ◽

Sandra Maria Conceição Pinheiro ◽

Fabíola Eloy da Rocha Medrado

Keyword(s):

Serum Ferritin ◽

Erythrocyte Indices

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Association of body mass index with hemoglobin level, erythrocyte indices and red cell distribution width.

The Professional Medical Journal ◽

10.29309/tpmj/2021.28.03.5334 ◽

2021 ◽

Vol 28 (03) ◽

pp. 311-317

Author(s):

Saleemullah Abro ◽

Qurratulain Saleem ◽

Syeda Asiya Parveen ◽

Ali Ahmed ◽

Syed Hafeezul Hussan ◽

...

Keyword(s):

Body Mass Index ◽

Body Mass ◽

Hemoglobin Level ◽

Red Cell Distribution Width ◽

Deficiency Anemia ◽

Red Cell ◽

Cell Distribution ◽

Distribution Width ◽

Erythrocyte Indices ◽

Study Participants

Objectives: Objective of this study is to evaluate the association of body mass index with hemoglobin Level, erythrocyte Indices and red cell distribution width in medical students of Baqai Medical University Karachi. Study Design: Descriptive cross - sectional study. Setting: Physiology Department of Baqai Medical University Karachi. Period: 15 February 2017 to 15 August 2017. Material & Methods: A total of 500 students were enrolled in this study. The anthropometric measurement [height (m2) and weight (kg)] was recorded for calculation of the Body Mass Index and Complete blood count i-e Hemoglobin%, erythrocyte indices {Mean corpuscular volume(MCV), Mean corpuscular hemoglobin(MCH), Mean corpuscular hemoglobin concentration (MCHC), Red cell distribution width (RDW) were done and Mentzer’s Index was calculated. Statistical Package for the Social Sciences (SPSS) version 22.0 was used to analyze the collected data. Results: It was observed in 500 medical students that 36% of the students were having low hemoglobin % level, while low values of erythrocyte indices (MCH: 38.0%, MCV 36.6% and MCHC 39.0% respectively) and 93.8% of participants had a higher values of RDW in study participants. Iron deficiency anemia (87.2%) and thalassemia (carrier or minor: 12.8%) were determined by using Menterzer Index. Mean body mass index of study participants were 23.04±3.68 and it was observed in study participants that only RDW was significantly associated with obese students (X²=9.878, p=0.02). Conclusion: With regard to our study results, higher percentage (87.2%) of Iron deficiency anemia were observed and having association between red cell distribution width to obesity.

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