scholarly journals Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal

2012 ◽  
Vol 19 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Nilanjan Chakraborty ◽  
Debarupa Banerjee ◽  
Moumita Ghosh ◽  
Prakash Pradhan ◽  
Namrata Shanu Gupta ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Withania Somnifera

Differential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudiana

Biologia ◽  
2017 ◽  
Vol 72 (10) ◽  
Author(s):  
Rabia Javed ◽  
Buhara Yucesan ◽  
Muhammad Zia ◽  
Ekrem Gurel
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Antioxidant Capacity ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Biotic Stress ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Defence Mechanism ◽  
Differential Effects

AbstractPlants naturally produce secondary metabolites on exposure to abiotic/biotic stress. Production of secondary metabolites, like phenols or flavonoids, is a defence mechanism against different stresses. This study explores the influence of plant growth regulators (PGRs) on the growth and secondary metabolites of

scholarly journals Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

AMB Express ◽  
2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Fatemeh Ahmadpoor ◽  
Nasser Zare ◽  
Rasool Asghari ◽  
Parisa Sheikhzadeh
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Cultures ◽  
Melia Azedarach ◽  
Ms Medium ◽  
Fresh Weight ◽  
Metabolites Production

AbstractMelia azedarach L. is a valuable source of antioxidants and secondary metabolites. This study is a first extensive report about the effect of different serialization protocols and plant growth regulators (PGRs) on explant disinfection efficiency, callus induction and secondary metabolites production and accumulation in callus cultures of M. azedarach L. In this regard, the effect of plant growth regulators on callus induction and secondary metabolites production were examined. In addition, different sterilization agents were evaluated for disinfection of chinaberry leaf explants. The results showed that the lowest percentage of explant contamination and browning with the highest percentage of callus induction and callus growth obtained with explants pretreated with benomyl (2 g/L) for 2 h and sterilized with 7% H2O2 for 10 min and NaOCl 2% (without pH adjustment) for 12 min. Although adjusting the pH of NaOCl to pH  = 7 and 10 significantly reduced the microbial contamination and increased the percentage of contamination-free cultures of M. azedarach L., adversely influenced the explant viability and callus induction and growth. The highest percentage of callus induction obtained on the MS medium containing 3 mg/L NAA/2,4-D and 1 or 3 mg/L Kin/BAP, and the highest callus yield (1804.833 mg/explant) belonged to the MS medium supplemented with 5 mg/L 2,4-D and 5 mg/L Kin. The callus cultures grown on the MS medium supplemented with 3 mg/L NAA and 1 mg/L Kin produced the highest amount of Quercetin (2.06 mg/g fresh weight), Rutin (5.56 mg/g fresh weight) and Kaempferol (1.84 mg/g fresh weight).

The Effects of NH4+ and NO3− and Plant Growth Regulators on the Accumulation of Nutrients, Carbohydrates and Secondary Metabolites of Stevia rebaudiana Bertoni

Sugar Tech ◽  
2020 ◽  
Author(s):  
Hourieh Tavakoli Hasanaklou ◽  
Ali Ebadi Khazineh Ghadim ◽  
Foad Moradi ◽  
Sodabeh Jahanbakhsh Ghodehkahriz ◽  
Abdolghayoum Gholipouri
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni

scholarly journals Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Sarieh TARIGHOLIZADEH ◽  
Rouhollah MOTAFAKKERAZAD ◽  
Morteza KOSARI-NASAB ◽  
Ali MOVAFEGHI ◽  
Sakineh MOHAMMADI ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Total Phenolics ◽  
Fresh Mass

<p class="042abstractstekst">The impact of combinations of plant growth regulators (PGRs) on callus culture of <em>Satureja sahendica </em>Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l<sup>-1</sup>. The treatment of 2 mg l<sup>-1</sup> 2,4-D + 2 mg l<sup>-1 </sup>Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g<sup>-1</sup>) and flavonoids (24.903 ± 7.016 mg QE g<sup>-1</sup>). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of <em>Satureja sahendica</em> was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.</p>

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