Occurrence of aflatoxin M1 in commercial pasteurized milk samples in Sari, Mazandaran province, Iran

2016 ◽  
Vol 32 (2) ◽  
pp. 85-87 ◽  
Author(s):  
Hamidreza Mohammadi ◽  
Mohammad Shokrzadeh ◽  
Zahra Aliabadi ◽  
Bamdad Riahi-Zanjani
Keyword(s):  
Aflatoxin M1 ◽  
Mazandaran Province ◽  
Pasteurized Milk ◽  
Milk Samples

Incidence of Aflatoxin M1 in Thai Milk Products

1997 ◽  
Vol 60 (8) ◽  
pp. 1010-1012 ◽  
Author(s):  
KRIENGSAG SAITANU
Keyword(s):  
High Temperature ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Test Results ◽  
Powdered Milk ◽  
Milk Products ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Two Samples ◽  
Sterilized Milk

Two hundred seventy samples of raw milk and off-the-shelf milk products were examined for aflatoxin M1 content using a radioimmunoassay. Aflatoxin M1 was found in the majority of milk samples except 1 sample of raw milk and 11 samples of imported powdered milk. All cases of aflatoxin M1 content greater than 0.5 ppb were found in 18% (48) of the samples including raw milk (17/67), pasteurized milk (20/63), ultra high temperature milk (7/60), sterilized milk (3/60), and pelleted milk (1/7). All powdered milk samples were negative for aflatoxin M1 except two samples with less than 0.1 ppb. The positive aflatoxin M1 test results for five of the raw milk samples were confirmed by HPLC.

Occurrence of Aflatoxins in Peanuts, Milk, and Animal Feed in Trinidad

2007 ◽  
Vol 70 (3) ◽  
pp. 771-775 ◽  
Author(s):  
NKECHI OFFIAH ◽  
ABIODUN ADESIYUN
Keyword(s):  
Animal Feed ◽  
Peanut Butter ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Public Health Importance ◽  
Dairy Farmers ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Radioimmunoassay Method ◽  
Feed Samples

The prevalence of aflatoxin B1 (AFB1) in 186 peanut products (140 peanuts, 32 peanut butter, and 14 nut cakes) from supermarkets, road vendors, and sale outlets, and 40 feed samples from dairy farmers was determined using the radioimmunoassay method (Charm II) test for aflatoxins. The frequency of aflatoxin M1 (AFM1) was also determined in 175 raw milk samples from milk collection centers and 37 pasteurized milk samples obtained from supermarkets and sale outlets. Overall, from a total of 438 samples tested, 18 (4.1%) were positive for aflatoxin comprising 5 (2.2%) of 226 peanut products and feeds positive for AFB1, and 13 (6.1%) of 212 milk samples positive for AFM1. All 186 peanuts and peanut products were negative (0.0%) for AFB1 while 5 (7.4%) of 40 dairy feed samples were positive. Of the 175 raw milk samples tested, 13 (7.4%) were contaminated with AFM1 while all pasteurized milk samples were negative. The detection of AFB1 in feed and AFM1 in milk is of public health importance considering the practice of raw milk consumption by the farmers and their families in the country.

Occurrence of Aflatoxin M1 in Pasteurized Milk of the School Milk Project in Thailand

2009 ◽  
Vol 72 (8) ◽  
pp. 1761-1763 ◽  
Author(s):  
SUTHEP RUANGWISES ◽  
NONGLUCK RUANGWISES
Keyword(s):  
Rainy Season ◽  
High Performance ◽  
Aflatoxin M1 ◽  
Milk Products ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Regulatory Limits ◽  
The Status ◽  
The Mean ◽  
Quality Of Milk

Concentrations of aflatoxin M1 (AFM1) were determined in 150 pasteurized milk samples from the School Milk Project in Thailand. Milk samples were collected from 50 schools in the Central region of Thailand in three seasons: summer (May to June 2006), the rainy season (August to September 2006), and winter (December 2006 to January 2007). AFM1 was isolated by using an immunoaffinity column and quantified by high-performance liquid chromatography. All of the 150 pasteurized milk samples were contaminated with AFM1, and the concentrations were within the U.S. regulatory limit of 0.5 μg/liter. The highest concentration of AFM1 found in school milk samples was 0.114 μg/liter. The mean concentration of AFM1 in milk samples collected in winter was significantly higher than the mean concentrations found in the rainy season and summer. Further monitoring of school milk to evaluate the status of contamination of AFM1 is necessary, with a special emphasis on samples collected in the rainy season and winter. Thailand is one of several countries that have no regulatory limits for AFM1 in milk and milk products. The results of this study suggest that safety limits for AFM1 are needed for regulating and ensuring the quality of milk and milk products in Thailand.

scholarly journals Quantum bead-based fluorescence-linked immunosorbent assay for ultrasensitive detection of aflatoxin M1 in pasteurized milk, yogurt, and milk powder

2019 ◽  
Vol 102 (5) ◽  
pp. 3985-3993 ◽  
Author(s):  
Yaofeng Zhou ◽  
Sicheng Xiong ◽  
KangKang Zhang ◽  
Lin Feng ◽  
Xuelan Chen ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Milk Powder ◽  
Aflatoxin M1 ◽  
Ultrasensitive Detection ◽  
Pasteurized Milk

The concentration of aflatoxin M1 in raw and pasteurized milk: A worldwide systematic review and meta-analysis

2021 ◽  
Author(s):  
Inass Mollayusefian ◽  
Vahid Ranaei ◽  
Zahra Pilevar ◽  
Marina M.S. Cabral-Pinto ◽  
Ali Rostami ◽  
...  
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Aflatoxin M1 ◽  
Pasteurized Milk

Characterization of Serratia species and qualitative detection of Serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction

2021 ◽  
Vol 67 (2) ◽  
pp. 3453-3464
Author(s):  
Evelin Korcz ◽  
László Varga ◽  
Zoltán Kerényi
Keyword(s):  
Serratia Marcescens ◽  
Bacterial Species ◽  
Relevant Literature ◽  
Inhibitory Effect ◽  
Test Methods ◽  
In Vitro Assays ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Microbiological Methods

Serratia species are opportunistic pathogenic microorganisms primarily known as nosocomial infectious agents, which can also cause food quality problems. The appearance of the extracellular pigment-producing Serratia marcescens in cow’s milk causes its red discoloration, posing a challenge to the dairy industry and food certification laboratories. The detection of the bacterium by conventional procedures based on microbiological methods is time-consuming and labor-intensive, and in many cases does not lead to satisfactory results due to the competitive inhibitory effect of the accompanying microflora. Following the analysis of the relevant literature, the published endpoint PCR methods and the primers used for the detection of S. marcescens were evaluated in in silico and in vitro assays, and then the procedure was tested on farm milk samples. Using the method, a total of 60 raw and pasteurized milk samples were analyzed, more than half of which (i.e., 32) were identified as S. marcescens positive. The significance of our work is mainly represented by the application of the published test methods in food industry practice. Our results highlight to the importance of detecting this bacterial species.

Heat-Resistant Psychrotrophic Bacteria Isolated from Pasteurized Milk1

1977 ◽  
Vol 40 (2) ◽  
pp. 101-108 ◽  
Author(s):  
C. J. WASHAM ◽  
H. C. OLSON ◽  
E. R. VEDAMUTHU
Keyword(s):  
Thermal Resistance ◽  
Shelf Life ◽  
Detailed Examination ◽  
Genus Bacillus ◽  
Pasteurized Milk ◽  
Psychrotrophic Bacteria ◽  
Milk Samples ◽  
Heat Resistant ◽  
Different Types

Psychrotrophic bacteria were isolated from 227 pasteurized milk samples which had a shelf life in excess of 20 days at 7.2 C. Of 700 cultures isolated, 135 were resistant to heating at 72 C for 16 sec and were able to re-establish growth at 7.2 C. Thirty-five cultures, representing 15 different types were subjected to detailed examination to determine their actions on refrigerated milk, growth temperatures, thermal resistance at various temperatures, and their identities. The spore-forming genus Bacillus occured most frequently. The non-sporing types were assigned to the genera Arthrobacter, Microbacterium, Streptococcus, and Corynebacterium.

scholarly journals The Occurrence of Aflatoxin M1 in Milk, soft cheese and yoghurt in Baghdad Province by Using ELISA Test

2014 ◽  
Vol 38 (2) ◽  
pp. 9-16
Author(s):  
Najim Hadi Najim
Keyword(s):  
Dairy Products ◽  
Raw Milk ◽  
Elisa Test ◽  
Mean Value ◽  
Aflatoxin M1 ◽  
Contamination Level ◽  
Milk Samples ◽  
White Cheese ◽  
The Mean ◽  
Milk And Dairy Products

     Milk and dairy products are fundamental components in the human diet and may be the principle way for entrance of Aflatoxin M1 (AFM1) in to the human body. All milk and dairy products samples were tested for the occurrence of AFM1 by the competitive ELISA technique. Out of 32 bovine raw milk samples that were collected from eight villages around Baghdad province, 32 samples (100 %) were contaminated with AFM1 ranging from 0.15 to 86.96ng/kg with mean value of 42.37±26.07 ng/kg, of which 17 samples were contaminated with concentrations < 50 ng/kg and 15 samples exceeded the maximum acceptable level of AFM1 in milk (50 ng/kg) imposed by the European legislation. The raw milk samples belonged to animals fed with composite and stored fodder as in Althahab Alabiadh, Radhwaniya and Fadhaliya villages had higher significantly AFM1 concentrations over all the other five villages (Grazing feed). All 32 (100%) locally produced soft white cheese samples analyzed were contaminated with AFM1 ranging from 31.84 to 89.44 ng/kg with the mean value of 59.92±17.03 ng/kg. Out of 32 locally produced yoghurt samples analyzed, 32 samples (100%) were contaminated with AFM1 ranging from 0.16 to 42.74 ng/kg with the mean value of 16.92±11.55 ng/kg. Thirty samples (100%) of the examined 30 imported UHT milk samples that were collected from different commercial companies in the province of Baghdad presented significantly  high contamination level with AFM1 that were found to range from 0.18 to 85.66 ng/kg.

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