Structural transformation of oyster, hard clam, and sea urchin shells after calcination and their antibacterial activity against foodborne microorganisms

2015 ◽  
Vol 81 (4) ◽  
pp. 787-794 ◽  
Author(s):  
Yu-Chun Chen ◽  
Chun-Lan Lin ◽  
Chih-Ting Li ◽  
Deng-Fwu Hwang
Keyword(s):  
Antibacterial Activity ◽  
Structural Transformation ◽  
Sea Urchin ◽  
Hard Clam

Antibacterial Activity of Hydroalcoholic Extract of Sea Urchin Temnopleurus Alexandri (Bell, 1884)

2011 ◽  
Vol 4 (1) ◽  
pp. 557-559
Author(s):  
R. Parvathavarthini R. Parvathavarthini ◽  
◽  
Dr. B.Uma Dr. B.Uma
Keyword(s):  
Antibacterial Activity ◽  
Sea Urchin ◽  
Hydroalcoholic Extract

scholarly journals Potential Bacterial Symbion of Sea Urchin As a Multi-Drug Resistant (MDR) Antibacterial Agent Against Staphylococcus aureus and Escherichia Coli Bacteria

2018 ◽  
Vol 23 (3) ◽  
pp. 131
Author(s):  
Suzana Kristy Satriani Fofied ◽  
Agus Sabdono ◽  
Diah Permata Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Sea Urchin ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Symbiotic Bacteria ◽  
Dilution Method ◽  
Drug Resistant ◽  
Escherichia Coli Bacteria

Staphylococcus aureus and Escherichia coli are pathogenic bacteria agent of many human diseases. Those bacteria infect in various levels and also been antibiotic resistants. Bacterial resistance has become a serious global problem. The purposes of this study were to isolate and identify the symbiotic bacteria of the Sea Urchin that have an antibacterial activity of the Strain Multi-Drug Resistant (MDR) against Staphylococcus aureus and Escherichia coli. Sea Urchin was taken from Panjang island, Jepara Indonesia at 2-3 m depth. The symbiotic bacteria were isolated from Sea Urchin by using dilution method and spread plate method. Phenotypic characteristics was observed on colony shape, color and texture of growing bacteria. While the streak method was used to purify bacterial symbion. The antibacterial activity test was performed using overlay method. The results showed that 3 out of 37 isolates have antibacterial activity against Staphylococcus aureus and Escherichia coli. The BB.03.35 isolate was selected for molecular identification due to the largest inhibitory zone diameter. The sequence of this bacterium showed 97% homology and closely related to Pseudoalteromonas flavipulchra.

scholarly journals Isolation and Characterization of Antibacterial Compounds from Sea Urchin

2015 ◽  
Vol 18 (1) ◽  
Author(s):  
Febrina Olivia Akerina ◽  
Tati Nurhayati ◽  
Ruddy Suwandi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Sea Urchin ◽  
Proximate Composition ◽  
Bioactive Compound ◽  
Body Part ◽  
Main Research ◽  
E Coli ◽  
Isolation And Characterization ◽  
Preliminary Research

Sea urchin is highly valued seafood. Its bioactive compound also has a potential as natural antibacterial agent. The aim of this research was to screen the antibacterial activity using cup-plate method, toxicity potential using brine shrimp lethality test (BSLT) method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50) from the three gonads extract was 471.861 ppm (n-hexane), 563.226 ppm (ethyl acetate) and 577.531 ppm (methanolic), respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli. Keyword : Antibacteria, bioactive compound, D. setosum, sea urchin

scholarly journals In vitro antibacterial activity and potential applications in food of sea urchin (Diadema setosum) from Cape of Palette, South Sulawesi

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2139-2146
Author(s):  
M. Yusuf ◽  
U.A. Fitriani Nur ◽  
A. Rifai
Keyword(s):  
Antibacterial Activity ◽  
Sea Urchin ◽  
Methanol Extract ◽  
Marine Invertebrates ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Ultrasound Assisted Extraction ◽  
E Coli ◽  
Diadema Setosum

Marine invertebrates in support of his life's defence of sea predatory use an innate immune mechanism, namely the cellular component hemocytes by secreting the dissolved antimicrobial and cytotoxic substances. It shows that marine invertebrates are potential sources and promising antimicrobial compounds. This research was aimed at determining the antibacterial activity of sea urchin (Diadema setosum) extract against some bacterial isolates and its application to foodstuffs. The gonad and shell of sea urchin extracted by methanol and ethyl acetate and then separated by ultrasound-assisted extraction. Screening of antibacterial compound use gas chromatography-mass spectrometry (GC-MS), and disc diffusion method was followed to determine the antimicrobial activity against Salmonella, Escherichia coli and Staphylococcus aureus. The results of this study showed antibacterial activity against one or more strains. The gonad of sea urchin from methanol extract exhibited significant inhibitory effect and effective against Salmonella, E. coli and S. aureus. Majority of gonad and shell of sea urchin extract showed antibacterial activity against the tested strains. However, gonad of methanol extract was found to be inhibiting microorganisms gram-negative (E. coli) and gram-positive (Salmonella and S. aureus). Gonad extract can be a good source of antibacterial agents.

Synthesis and characterization of whitlockite from sea urchin skeleton and investigation of antibacterial activity

2021 ◽  
Vol 47 (1) ◽  
pp. 626-633
Author(s):  
Ayşegül Yücel ◽  
Selda Sezer ◽  
Emre Birhanlı ◽  
Tuba Ekinci ◽  
Emine Yalman ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Sea Urchin ◽  
Synthesis And Characterization

Cellular distribution of sea urchin antibacterial activity

1990 ◽  
Vol 70 (3) ◽  
pp. 153-157 ◽  
Author(s):  
Patrizia Gerardi ◽  
Maguy Lassegues ◽  
Calogero Canicatti
Keyword(s):  
Antibacterial Activity ◽  
Sea Urchin ◽  
Cellular Distribution

The structure of pre-mRNA and mRNA from erythroid cells

1978 ◽  
Vol 36 (2) ◽  
pp. 234-235
Author(s):  
A.-M. Ladhoff ◽  
B.J. Thiele ◽  
Ch. Coutelle ◽  
S. Rosenthal
Keyword(s):  
Bone Marrow ◽  
Structural Transformation ◽  
Electron Micrographs ◽  
Ammonium Acetate ◽  
Bone Marrow Cells ◽  
Erythroid Cells ◽  
Globin Mrna ◽  
Ordered Structures ◽  
Rabbit Bone ◽  
Rabbit Reticulocytes

The suggested precursor-product relationship between the nuclear pre-mRNA and the cytoplasmic mRNA has created increased interest also in the structure of these RNA species. Previously we have been published electron micrographs of individual pre-mRNA molecules from erythroid cells. An intersting observation was the appearance of a contour, probably corresponding to higher ordered structures, on one end of 10 % of the pre-mRNA molecules from erythroid rabbit bone marrow cells (Fig. 1A). A virtual similar contour was observed in molecules of 9S globin mRNA from rabbit reticulocytes (Fig. 1B). A structural transformation in a linear contour occurs if the RNA is heated for 10 min to 90°C in the presence of 80 % formamide. This structural transformation is reversible when the denatured RNA is precipitated and redissolved in 0.2 M ammonium acetate.

Immunogold and immunoblot studies on a cell surface protein found in primary mesenchyme cells and in the cortical secretory vesicles of the sea urchin egg

1987 ◽  
Vol 45 ◽  
pp. 832-833
Author(s):  
G.L. Decker ◽  
M.C. Valdizan
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Protein ◽  
Egg Cell ◽  
Secretory Vesicles ◽  
Cell Surface Protein ◽  
Surface Complexes ◽  
Mesenchyme Cells ◽  
Lowicryl K4m ◽  
Primary Mesenchyme Cells

A monoclonal antibody designated MAb 1223 has been used to show that primary mesenchyme cells of the sea urchin embryo express a 130-kDa cell surface protein that may be directly involved in Ca2+ uptake required for growth of skeletal spicules. Other studies from this laboratory have shown that the 1223 antigen, although in relatively low abundance, is also expressed on the cell surfaces of unfertilized eggs and on the majority of blastomeres formed prior to differentiation of the primary mesenchyme cells.We have studied the distribution of 1223 antigen in S. purpuratus eggs and embryos and in isolated egg cell surface complexes that contain the cortical secretory vesicles. Specimens were fixed in 1.0% paraformaldehyde and 1.0% glutaraldehyde and embedded in Lowicryl K4M as previously reported. Colloidal gold (8nm diameter) was prepared by the method of Mulpfordt.

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