Oxygen-Limiting Growth Conditions and Deletion of the Transition State Regulator Protein Abrb in Bacillus subtilis 6633 Result in an Increase in Subtilosin Production and a Decrease in Subtilin Production

2019 ◽  
Vol 12 (2) ◽  
pp. 725-731 ◽  
Author(s):  
Torsten Stein
Keyword(s):  
Bacillus Subtilis ◽  
Transition State ◽  
Growth Conditions ◽  
Regulator Protein ◽  
State Regulator ◽  
Transition State Regulator

A dual mode of regulation of flgM by ScoC in Bacillus subtilis

2009 ◽  
Vol 55 (8) ◽  
pp. 983-989 ◽  
Author(s):  
Prashant Kodgire ◽  
K. Krishnamurthy Rao
Keyword(s):  
Bacillus Subtilis ◽  
Transition State ◽  
Promoter Region ◽  
Dual Mode ◽  
State Regulator ◽  
Transition State Regulator ◽  
Dependent Pathway ◽  
Mode Of Regulation

In Bacillus subtilis , the transition state regulator ScoC indirectly, negatively regulates the anti-σD factor FlgM in a SinR-dependent pathway leading to an increased availability of σD. In addition to the SinR-dependent pathway, ScoC negatively regulates FlgM via directly repressing flgM transcription by binding to two sites in the promoter region of the flgM operon. Our studies also show that the regulation of FlgM by SinR is not at the transcriptional or translational levels. Thus, ScoC shows a dual mode of downregulation of FlgM, via both SinR-dependent and -independent pathways, which eventually results in the increased σD activity.

scholarly journals Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation

2010 ◽  
Vol 39 (2) ◽  
pp. 414-428 ◽  
Author(s):  
Onuma Chumsakul ◽  
Hiroki Takahashi ◽  
Taku Oshima ◽  
Takahiro Hishimoto ◽  
Shigehiko Kanaya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Transcriptional Regulation ◽  
Transition State ◽  
Genome Wide ◽  
State Regulator ◽  
Transition State Regulator

scholarly journals hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC

Microbiology ◽  
2009 ◽  
Vol 155 (1) ◽  
pp. 142-149 ◽  
Author(s):  
Prashant Kodgire ◽  
K. Krishnamurthy Rao
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Transition State ◽  
Negative Regulation ◽  
Positive Regulation ◽  
State Regulator ◽  
Transition State Regulator

In Bacillus subtilis, motility and chemotaxis require the expression of hag, which encodes flagellin. This gene is transcribed by the σ D form of RNA polymerase and is regulated by a group of proteins called transition state regulators (TSRs). Our studies show that hag transcription is negatively regulated by the transition state regulator ScoC, by binding to its promoter. Furthermore, ScoC, indirectly, also positively regulates hag by increasing the availability of σ D by downregulating the levels of the anti-σ D-factor FlgM. We further show that the positive regulation by ScoC predominates over the negative regulation.

Behavior of Transition State Regulator AbrB in Batch Cultures of Bacillus thuringiensis

2014 ◽  
Vol 69 (5) ◽  
pp. 725-732 ◽  
Author(s):  
Astrid Magdalena Lozano Goné ◽  
Jabel Dinorín Téllez Girón ◽  
Fabiola Eloisa Jiménez Montejo ◽  
María Eugenia Hidalgo-Lara ◽  
Víctor Eric López y López
Keyword(s):  
Bacillus Thuringiensis ◽  
Transition State ◽  
Batch Cultures ◽  
State Regulator ◽  
Transition State Regulator

scholarly journals ScoC Regulates Bacilysin Production at the Transcription Level in Bacillus subtilis

2009 ◽  
Vol 191 (23) ◽  
pp. 7367-7371 ◽  
Author(s):  
Takashi Inaoka ◽  
Guojun Wang ◽  
Kozo Ochi
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Engineering ◽  
Genome Sequencing ◽  
Promoter Region ◽  
Sequencing Analysis ◽  
Transcription Level ◽  
Mobility Shift ◽  
State Regulator ◽  
Transition State Regulator ◽  
Gel Mobility Shift

ABSTRACT Bacillus subtilis mutants with high expression of the bacilysin operon ywfBCDEFG were isolated. Comparative genome sequencing analysis revealed that all of these mutants have a mutation in the scoC gene. The disruption of scoC by genetic engineering also resulted in increased expression of ywfBCDEFG. Primer extension and gel mobility shift analyses showed that the ScoC protein binds directly to the promoter region of ywfBCDEFG. Our results indicate that the transition state regulator ScoC, together with CodY and AbrB, negatively regulates bacilysin production in B. subtilis.

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