Analysis of quantitative trait loci associated with seed germination and coleoptile length under low temperature condition

2015 ◽  
Vol 18 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Joohyun Lee ◽  
Soon Wook Kwon
Keyword(s):  
Quantitative Trait Loci ◽  
Seed Germination ◽  
Low Temperature ◽  
Quantitative Trait ◽  
Coleoptile Length ◽  
Trait Loci

scholarly journals Characterization of Quantitative Trait Loci for Germination and Coleoptile Length under Low-Temperature Condition Using Introgression Lines Derived from an Interspecific Cross in Rice

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1200
Author(s):  
Mirjalol Akhtamov ◽  
Cheryl Adeva ◽  
Kyu-Chan Shim ◽  
Hyun-Sook Lee ◽  
Sun Ha Kim ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Low Temperature ◽  
Quantitative Trait ◽  
Single Gene ◽  
Interspecific Cross ◽  
Introgression Lines ◽  
Oryza Rufipogon ◽  
Isogenic Line ◽  
Coleoptile Length ◽  
Trait Loci

Previously, five putative quantitative trait loci (QTLs) for low-temperature germination (LTG) have been detected using 96 BC3F8 lines derived from an interspecific cross between the Korean japonica cultivar “Hwaseong” and Oryza rufipogon. In the present study, two introgression lines, CR1517 and CR1518, were used as parents to detect additional QTLs and analyze interactions among QTLs for LTG. The F2 population (154 plants) along with parental lines, Hwaseong and O. rufipogon, were evaluated for LTG and coleoptile length under low-temperature conditions (13 °C). Among five QTLs for LTG, two major QTLs, qLTG1 and qLTG3, were consistently detected at 6 and 7 days after incubation. Three minor QTLs were detected on chromosomes 8 and 10. Two QTLs, qLTG10.1 and qLTG10.2, showing linkage on chromosome 10, exerted opposite effects with the Hwaseong allele at qLTG10.2 and the O. rufipogon allele at qLTG10.1 respectively, in turn, increasing LTG. Interactions among QTLs were not significant, implying that the QTLs act in an additive manner. Near-isogenic line plants with the combination of favorable alleles from O. rufipogon and Hwaseong exhibited higher LTG than two introgression lines. With regard to coleoptile length, three QTLs observed on chromosomes 1, 3, and 8 were colocalized with QTLs for LTG, suggesting the pleiotropy of the single gene at each locus. According to the results, the introgression of favorable O. rufipogon alleles could hasten the development of rice with high LTG and high coleoptile elongation in japonica cultivars.

Identification of Quantitative Trait Loci for ABA Sensitivity at Seed Germination and Seedling Stages in Rice

2006 ◽  
Vol 33 (6) ◽  
pp. 532-541 ◽  
Author(s):  
Jun YOU ◽  
Qiang LI ◽  
Bing YUE ◽  
Wei-Ya XUE ◽  
Li-Jun LUO ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Seed Germination ◽  
Quantitative Trait ◽  
Aba Sensitivity ◽  
Trait Loci

Quantitative trait loci associated with lettuce seed germination under different temperature and light environments

Genome ◽  
2008 ◽  
Vol 51 (11) ◽  
pp. 928-947 ◽  
Author(s):  
Eiji Hayashi ◽  
Natsuyo Aoyama ◽  
David W. Still
Keyword(s):  
Quantitative Trait Loci ◽  
Seed Germination ◽  
Path Analysis ◽  
Quantitative Trait ◽  
Germination Rate ◽  
Red Light ◽  
Seed Maturation ◽  
Lettuce Seed ◽  
Factors Affecting ◽  
Trait Loci

Temperature and light are primary environmental cues affecting seed germination. To elucidate the genetic architecture underlying lettuce ( Lactuca sativa L.) seed germination under different environmental conditions, an F8 recombinant inbred line population consisting of 131 families was phenotyped for final germination and germination rate. Seeds were imbibed in water at 20 °C under continuous red light (20-Rc), 20 °C continuous dark (20-Dc), 31.5 °C continuous red light (31.5-Rc), 31.5 °C continuous dark (31.5-Dc), or 20 °C far-red light for 24 h followed by continuous dark (20-FRc-Dc). Thirty-eight quantitative trait loci (QTL) were identified from two seed maturation environments: 10 for final germination and 28 for germination rate. The amount of variation attributed to an individual QTL ranged from 9.3% to 17.2% and from 5.6% to 26.2% for final germination and germination rate, respectively. Path analysis indicated that factors affecting germination under 31.5-Rc or 31.5-Dc are largely the same, and these appear to differ from those employed under 20-FRc-Dc. QTL and path analysis support the notion of common and unique factors for germination under diverse temperature and light regimes. A highly significant effect of the seed maturation environment on subsequent germination capacity under environmental stress was observed.

Quantitative trait loci mapping and stability for low temperature germination ability of rice

2009 ◽  
Vol 128 (4) ◽  
pp. 387-392 ◽  
Author(s):  
S. L. Ji ◽  
L. Jiang ◽  
Y. H. Wang ◽  
W. W. Zhang ◽  
X. Liu ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Low Temperature ◽  
Quantitative Trait ◽  
Quantitative Trait Loci Mapping ◽  
Germination Ability ◽  
Trait Loci

scholarly journals Identification of main-effect quantitative trait loci (QTLs) for low-temperature stress tolerance germination- and early seedling vigor-related traits in rice (Oryza sativa L.)

2020 ◽  
Vol 40 (1) ◽  
Author(s):  
S. Najeeb ◽  
J. Ali ◽  
A. Mahender ◽  
Y.L. Pang ◽  
J. Zilhas ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Low Temperature ◽  
Quantitative Trait ◽  
Root Length ◽  
Stress Index ◽  
Multiple Traits ◽  
Main Effect ◽  
Trait Loci ◽  
Early Seedling ◽  
Genomic Regions

AbstractAn attempt was made in the current study to identify the main-effect and co-localized quantitative trait loci (QTLs) for germination and early seedling growth traits under low-temperature stress (LTS) conditions in rice. The plant material used in this study was an early backcross population of 230 introgression lines (ILs) in BCIF7 generation derived from the Weed Tolerant Rice-1 (WTR-1) (as the recipient) and Haoannong (HNG) (as the donor). Genetic analyses of LTS tolerance revealed a total of 27 main-effect quantitative trait loci (M-QTLs) mapped on 12 chromosomes. These QTLs explained more than 10% of phenotypic variance (PV), and average PV of 12.71% while employing 704 high-quality SNP markers. Of these 27 QTLs distributed on 12 chromosomes, 11 were associated with low-temperature germination (LTG), nine with low-temperature germination stress index (LTGS), five with root length stress index (RLSI), and two with biomass stress index (BMSI) QTLs, shoot length stress index (SLSI) and root length stress index (RLSI), seven with seed vigor index (SVI), and single QTL with root length (RL). Among them, five significant major QTLs (qLTG(I)1, qLTGS(I)1–2, qLTG(I)5, qLTGS(I)5, and qLTG(I)7) mapped on chromosomes 1, 5, and 7 were associated with LTG and LTGS traits and the PV explained ranged from 16 to 23.3%. The genomic regions of these QTLs were co-localized with two to six QTLs. Most of the QTLs were growth stage-specific and found to harbor QTLs governing multiple traits. Eight chromosomes had more than four QTLs and were clustered together and designated as promising LTS tolerance QTLs (qLTTs), as qLTT1, qLTT2, qLTT3, qLTT5, qLTT6, qLTT8, qLTT9, and qLTT11. A total of 16 putative candidate genes were identified in the major M-QTLs and co-localized QTL regions distributed on different chromosomes. Overall, these significant genomic regions of M-QTLs are responsible for multiple traits and this suggested that these could serve as the best predictors of LTS tolerance at germination and early seedling growth stages. Furthermore, it is necessary to fine-map these regions and to find functional markers for marker-assisted selection in rice breeding programs for cold tolerance.

Quantitative trait loci controlling rice seed germination under salt stress

Euphytica ◽  
2010 ◽  
Vol 178 (3) ◽  
pp. 297-307 ◽  
Author(s):  
Zhoufei Wang ◽  
Jianfei Wang ◽  
Yongmei Bao ◽  
Yunyu Wu ◽  
Hongsheng Zhang
Keyword(s):  
Salt Stress ◽  
Quantitative Trait Loci ◽  
Seed Germination ◽  
Quantitative Trait ◽  
Rice Seed ◽  
Trait Loci

scholarly journals Quantitative trait loci for cold tolerance of rice recombinant inbred lines in low temperature environments

2011 ◽  
Vol 32 (6) ◽  
pp. 579-587 ◽  
Author(s):  
Wenzhu Jiang ◽  
Yong-Mei Jin ◽  
Joohyun Lee ◽  
Kang-Ie Lee ◽  
Rihua Piao ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Trait Loci

Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.)

2001 ◽  
Vol 52 (12) ◽  
pp. 1221 ◽  
Author(s):  
G. J. Rebetzke ◽  
R. Appels ◽  
A. D. Morrison ◽  
R. A. Richards ◽  
G. McDonald ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Area ◽  
Plant Height ◽  
Quantitative Trait ◽  
Dwarfing Genes ◽  
Genotypic Variance ◽  
Coleoptile Length ◽  
Dwarfing Gene ◽  
Early Vigour ◽  
Trait Loci

The Norin-10 dwarfing genes, Rht-B1b (Rht1) and Rht-D1b (Rht2), are commonly used to reduce plant height and increase grain yield in wheat breeding programs. These dwarfing genes lower sensitivity of vegetative tissue to endogenous gibberellin to reduce cell and subsequent stem elongation. This reduction in cell elongation capacity reportedly results in a concomitant reduction in coleoptile length and early vigour (leaf area) thereby affecting seedling establishment and growth. A detailed genetic map from a cross between tall Halberd (Rht-B1a) and semidwarf Cranbrook (Rht-B1b) wheat cultivars was used to assess genetic factors affecting seedling growth. Parental and 150 doubled haploid progeny lines were characterised for seedling and height-related traits in controlled and field environments. Genotypic variation was large and predominantly under additive genetic control with evidence for transgressive segregation for some traits. Narrow-sense heritabilities were moderate to high (h2 = 0.31–0.91) indicating a strong genetic basis for differences between progeny. Molecular marker analyses identified a number of significant (P < 0.05) quantitative trait loci (QTL) for each trait. A major QTL, mapping directly to the Rht-B1 locus on chromosome arm 4BS, accounted for up to 49% of the genotypic variance in peduncle length and plant height, and 27–45% of the genotypic variance in coleoptile length across different temperatures. Another QTL, located close to the RFLP marker XksuC2 on the long arm of chromosome 4B, accounted for 15–27% of the genotypic variance in coleoptile length. The influence of the XksuC2-linked QTL on coleoptile length was greatest at 19˚C and decreased with cooler temperatures. The same QTL affected reductions in leaf size, and both coleoptile tiller size and presence to affect overall seedling vigour. There was also some evidence for epistatic interactions influencing coleoptile tiller growth. Reductions in plant size at the Rht-B1b and XksuC2 loci were associated with presence of the Cranbrook 4B allele. The negative genetic effect of the Rht-B1b dwarfing gene on early growth of wheat confirms phenotypic evidence of a pleiotropic effect of Rht-B1b on establishment and early vigour. Genetic increases in coleoptile length and early leaf area development are likely to be limited in wheat populations containing the Rht-B1b dwarfing gene.

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