Antibiotic resistance and virulence genes in Enterococcus species isolated from raw and processed seafood

2021 ◽  
Author(s):  
Mine Çardak ◽  
Sine Özmen Toğay ◽  
Mustafa Ay ◽  
Onur Karaalioğlu ◽  
Özlem Erol ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Enterococcus Species

scholarly journals Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Xuehan Li ◽  
Tao Huang ◽  
Kai Xu ◽  
Chenglin Li ◽  
Yirong Li
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Virulence Genes ◽  
Mainland China ◽  
Geographical Location ◽  
Antibiotic Resistance Genes ◽  
Virulence Gene ◽  
Molecular Characteristics ◽  
Staphylococcal Protein A

Abstract Background There have been no reports regarding the molecular characteristics, virulence features, and antibiotic resistance profiles of Staphylococcus aureus (S. aureus) from Hainan, the southernmost province of China. Methods Two hundred twenty-seven S. aureus isolates, consisting of 76 methicillin-resistant S. aureus (MRSA) and 151 methicillin-susceptible S. aureus (MSSA), were collected in 2013–2014 and 2018–2019 in Hainan, and investigated for their molecular characteristics, virulence genes, antibiotic resistance profiles and main antibiotic resistance genes. Results Forty sequence types (STs) including three new STs (ST5489, ST5492 and ST5493), and 79 Staphylococcal protein A (spa) types were identified based on multilocus sequence typing (MLST) and spa typing, respectively. ST398 (14.1%, 32/227) was found to be the most prevalent, and the prevalence of ST398-MSSA increased significantly from 2013 to 2014 (5.5%, 5/91) to 2018–2019 (18.4%, 25/136). Seventy-six MRSA isolates were subject to staphylococcus chromosomal cassette mec (SCCmec) typing. SCCmec-IVa was the predominant SCCmec type, and specifically, ST45-SCCmec IVa, an infrequent type in mainland China, was predominant in S. aureus from Hainan. The antibiotic resistance profiles and antibiotic resistance genes of S. aureus show distinctive features in Hainan. The resistant rates of the MRSA isolates to a variety of antibiotics were significantly higher than those of the MSSA isolates. The predominant erythromycin and tetracycline resistance genes were ermC (90.1%, 100/111) and tetK (91.8%, 78/85), respectively. Eleven virulence genes, including the Panton-Valentine leukocidin (pvl) and eta, were determined, and the frequency of eta and pvl were found to be 57.3 and 47.6%. Such high prevalence has never been seen in mainland China before. Conclusion S. aureus isolates in Hainan have unique molecular characteristics, virulence gene and antibiotic resistance profiles, and main antibiotic resistance genes which may be associated with the special geographical location of Hainan and local trends in antibiotic use.

scholarly journals Influx of Enterococci and Associated Antibiotic Resistance and Virulence Genes from Ready-To-Eat Food to the Human Digestive Tract

2007 ◽  
Vol 73 (21) ◽  
pp. 6740-6747 ◽  
Author(s):  
Lilia Macovei ◽  
Ludek Zurek
Keyword(s):  
Antibiotic Resistance ◽  
Digestive Tract ◽  
Fast Food ◽  
Virulence Genes ◽  
Food Contamination ◽  
Gene Copy ◽  
Marker Genes ◽  
Enterococcus Casseliflavus ◽  
Fast Food Restaurants ◽  
Human Digestive Tract

ABSTRACT The influx of enterococcal antibiotic resistance (AR) and virulence genes from ready-to-eat food (RTEF) to the human digestive tract was assessed. Three RTEFs (chicken salad, chicken burger, and carrot cake) were sampled from five fast-food restaurants five times in summer (SU) and winter (WI). The prevalence of enterococci was significantly higher in SU (92.0% of salad samples and 64.0% of burger samples) than in WI (64.0% of salad samples and 24.0% of burger samples). The overall concentrations of enterococci during the two seasons were similar (∼103 CFU/g); the most prevalent were Enterococcus casseliflavus (41.5% of isolates) and Enterococcus hirae (41.5%) in WI and Enterococcus faecium (36.8%), E. casseliflavus (27.6%), and Enterococcus faecalis (22.4%) in SU. Resistance in WI was detected primarily to tetracycline (50.8%), ciprofloxacin (13.8%), and erythromycin (4.6%). SU isolates were resistant mainly to tetracycline (22.8%), erythromycin (22.1%), and kanamycin (13.0%). The most common tet gene was tet(M) (35.4% of WI isolates and 11.9% of SU isolates). The prevalence of virulence genes (gelE, asa1, cylA, and esp) and marker genes for clinical isolates (EF_0573, EF_0592, EF_0605, EF_1420, EF_2144, and pathogenicity island EF_0050) was low (≤12.3%). Genotyping of E. faecalis and E. faecium using pulsed-field gel electrophoresis revealed that the food contamination likely originated from various sources and that it was not clonal. Our conservative estimate (single AR gene copy per cell) for the influx of tet genes alone to the human digestive tract is 3.8 � 105 per meal (chicken salad). This AR gene influx is frequent because RTEFs are commonly consumed and that may play a role in the acquisition of AR determinants in the human digestive tract.

scholarly journals Association Between Presence of Virulence Genes and Antibiotic Resistance in ClinicalKlebsiella PneumoniaeIsolates

2016 ◽  
Vol 47 (4) ◽  
pp. 306-311 ◽  
Author(s):  
Safoura Derakhshan ◽  
Shahin Najar Peerayeh ◽  
Bita Bakhshi
Keyword(s):  
Antibiotic Resistance ◽  
Virulence Genes

scholarly journals Assessing the population dynamics of Escherichia coli in a metropolitan river after an extreme flood event

2016 ◽  
Vol 15 (2) ◽  
pp. 196-208 ◽  
Author(s):  
Nicole M. Masters ◽  
Aaron Wiegand ◽  
Jasmin M. Thompson ◽  
Tara L. Vollmerhausen ◽  
Eva Hatje ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Population Dynamics ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Wastewater Treatment Plants ◽  
Vero Cells ◽  
Flood Event ◽  
Typing Method ◽  
E Coli ◽  
Extreme Flood

We investigated Escherichia coli populations in a metropolitan river after an extreme flood event. Between nine and 15 of the 23 selected sites along the river were sampled fortnightly over three rounds. In all, 307 E. coli were typed using the PhP typing method and were grouped into common (C) or single (S) biochemical phenotypes (BPTs). A representative from each of the 31 identified C-BPTs was tested for 58 virulence genes (VGs) associated with intestinal and extra-intestinal E. coli, resistance to 22 antibiotics, production of biofilm and cytotoxicity to Vero cells. The number of E. coli in the first sampling round was significantly (P < 0.01) higher than subsequent rounds, whereas the number of VGs was significantly (P < 0.05) higher in isolates from the last sampling round when compared to previous rounds. Comparison of the C-BPTs with an existing database from wastewater treatment plants (WWTPs) in the same catchment showed that 40.6% of the river isolates were identical to the WWTP isolates. The relatively high number of VGs and antibiotic resistance among the C-BPTs suggests possessing and retaining these genes may provide niche advantages for those naturalised and/or persistent E. coli populations which may pose a health risk to the community.

Antibiotic Resistance and Virulence Genes in Enterococcus faecalis Isolated From Human Dental Plaque

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Mehrnaz Bakhti ◽  
Mona Akhondnezhad ◽  
Mehrdad Gholami ◽  
Mohtaram Nasrolahei ◽  
Hamid Reza Goli
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Dental Plaque ◽  
Virulence Genes

scholarly journals Molecular characterization and antibiotic resistance of Enterococcus species from gut microbiota of Chilean Altiplano camelids

2014 ◽  
Vol 4 (1) ◽  
pp. 24714 ◽  
Author(s):  
Katheryne Guerrero-Olmos ◽  
John Báez ◽  
Nicomédes Valenzuela ◽  
Joselyne Gahona ◽  
Rosa del Campo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gut Microbiota ◽  
Molecular Characterization ◽  
Enterococcus Species ◽  
Chilean Altiplano

scholarly journals The population genomics of increased virulence and antibiotic resistance in human commensal Escherichia coli over 30 years in France

2021 ◽  
Author(s):  
Julie Marin ◽  
Olivier Clermont ◽  
Guilhem Royer ◽  
Melanie Mercier-Darty ◽  
Jean-Winoc Decousser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
High Risk ◽  
Virulence Genes ◽  
Population Genomics ◽  
E Coli ◽  
O Antigen ◽  
Similar Region ◽  
Intestinal Infections ◽  
Evolution Of Virulence

Escherichia coli is a commensal species of the lower intestine, but also a major pathogen causing intestinal and extra-intestinal infections. Most studies on genomic evolution of E. coli used isolates from infections, and/or focused on antibiotic resistance, but neglected the evolution of virulence. Here instead, we whole-genome sequenced a collection of 436 E. coli isolated from fecal samples of healthy adult volunteers in France between 1980 and 2010. These isolates were distributed among 159 sequence types (STs), the five most frequent being ST10 (15.6%), ST73 (5.5%) and ST95 (4.8%), ST69 (3.7%) and ST59 (3.7%), and 230 O:H serotypes. ST and serotype diversity increased over time. Comparison with 912 E. coli bacteremia isolates from similar region and time showed a greater diversity in commensal isolates. The O1, O2, O6 and O25-groups used in bioconjugate O-antigen vaccine were found in only 63% of the four main STs associated with a high risk of bacteremia (ST69, ST73, ST95 and ST131). In commensals, STs associated with a high risk of bacteremia increased in frequency. Both extra-intestinal virulence-associated genes and resistance to antibiotics increased in frequency. Evolution of virulence genes was driven by both clonal expansion of STs with more virulence genes, and increases in frequency within STs, whereas the evolution of resistance was dominated by increases in frequency within STs. This study provides a unique picture of the phylogenomic evolution of E. coli in its human commensal habitat over a 30-year period and suggests that the efficacy of O-antigen vaccines would be threatened by serotype replacement.

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