Safety studies of polyethylene glycol–hydroxyapatite nanocomposites on Drosophila melanogaster: an in vivo model

2022 ◽  
Author(s):  
Pallavi Dan ◽  
Swetha Senthilkumar ◽  
Devanand Venkatsubbu Gopinath ◽  
Sahabudeen Sheik Mohideen
Keyword(s):  
Drosophila Melanogaster ◽  
Polyethylene Glycol ◽  
In Vivo Model ◽  
Safety Studies

Drosophila melanogaster as an in vivo model to study the potential toxicity of cerium oxide nanoparticles

2019 ◽  
Vol 490 ◽  
pp. 70-80 ◽  
Author(s):  
Vignesh Sundararajan ◽  
Pallavi Dan ◽  
Ajay Kumar ◽  
G. Devanand Venkatasubbu ◽  
Sahoko Ichihara ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cerium Oxide ◽  
Cerium Oxide Nanoparticles ◽  
In Vivo Model ◽  
Oxide Nanoparticles ◽  
Potential Toxicity

scholarly journals Arsenic-containing hydrocarbons are toxic in the in vivo model Drosophila melanogaster

Metallomics ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 2010-2014 ◽  
Author(s):  
S. Meyer ◽  
J. Schulz ◽  
A. Jeibmann ◽  
M. S. Taleshi ◽  
F. Ebert ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Toxicity ◽  
In Vivo Model

Arsenic-containing hydrocarbons cause developmental toxicity in Drosophila melanogaster.

scholarly journals Impact of probiotics on development and behaviour in Drosophila melanogaster – a potential in vivo model to assess probiotics

2019 ◽  
Vol 10 (2) ◽  
pp. 179-188 ◽  
Author(s):  
E. Gómez ◽  
F. Martín ◽  
A.M. Nogacka ◽  
N. Salazar ◽  
L. Aláez ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
In Vivo Model ◽  
Average Weight ◽  
Wild Type ◽  
Egg Survival ◽  
Experimental Conditions ◽  
Screening Process ◽  
Axenic Condition ◽  
Behavioural Aspects

In vitro models are frequently used in probiotic research. However, such models often fail to predict in vivo functionality and efficacy. This fact complicates the screening process for selecting the most suitable strains, prior to accomplish expensive animal studies and clinical intervention trials. Therefore, additional sensitive, discriminating and cost-effective models are needed to conduct preliminary assays before undertaking human intervention studies definitely proving efficacy. With this purpose in mind, we explored the potential of axenic Drosophila melanogaster populations as well as of these axenic flies treated with probiotic microbial strains as a model to test the effects of probiotics on a subset of developmental and behavioural traits. An axenic D. melanogaster progeny from the wild-type Canton S strain was obtained and its eggs were further developed until pupae eclosion occurred in growth medium containing either of two probiotic strains: Bifidobacterium animalis subsp. lactis Bb12 or Lactobacillus rhamnosus GG. Whereas B. animalis Bb12 colonised the flies, the capacity of L. rhamnosus LGG to colonise was considerably lower in our experimental conditions. Regarding the influence of microbial load on the flies’ development, the axenic condition caused a decrease in egg survival, and lowered adults’ average weight with respect to wild-type flies. Both probiotics were able to counteract these effects. An earlier emergence of adults was observed from eggs treated with L. rhamnosus GG in comparison to the other fly populations. The axenic condition did not influence negative geotaxis behaviour in Drosophila; however, flies mono-associated with B. animalis Bb12 moved faster than wild-type. Our results suggest that the use of axenic/probiotic-treated D. melanogaster populations may be an affordable model for preliminary testing of the effects of probiotics on developmental or behavioural aspects.

A comprehensive study of the harmful effects of ZnO nanoparticles using Drosophila melanogaster as an in vivo model

2015 ◽  
Vol 296 ◽  
pp. 166-174 ◽  
Author(s):  
Mohamed Alaraby ◽  
Balasubramanyam Annangi ◽  
Alba Hernández ◽  
Amadeu Creus ◽  
Ricard Marcos
Keyword(s):  
Drosophila Melanogaster ◽  
Zno Nanoparticles ◽  
In Vivo Model ◽  
Harmful Effects ◽  
Comprehensive Study

scholarly journals Iron sequestration by transferrin 1 mediates nutritional immunity in Drosophila melanogaster

2020 ◽  
Vol 117 (13) ◽  
pp. 7317-7325 ◽  
Author(s):  
Igor Iatsenko ◽  
Alice Marra ◽  
Jean-Philippe Boquete ◽  
Jasquelin Peña ◽  
Bruno Lemaitre
Keyword(s):  
Drosophila Melanogaster ◽  
Defense Mechanism ◽  
Fat Body ◽  
Immune Defense ◽  
In Vivo Model ◽  
Nutritional Immunity ◽  
Iron Sequestration

Iron sequestration is a recognized innate immune mechanism against invading pathogens mediated by iron-binding proteins called transferrins. Despite many studies on antimicrobial activity of transferrins in vitro, their specific in vivo functions are poorly understood. Here we use Drosophila melanogaster as an in vivo model to investigate the role of transferrins in host defense. We find that systemic infections with a variety of pathogens trigger a hypoferremic response in flies, namely, iron withdrawal from the hemolymph and accumulation in the fat body. Notably, this hypoferremia to infection requires Drosophila nuclear factor κB (NF-κB) immune pathways, Toll and Imd, revealing that these pathways also mediate nutritional immunity in flies. Next, we show that the iron transporter Tsf1 is induced by infections downstream of the Toll and Imd pathways and is necessary for iron relocation from the hemolymph to the fat body. Consistent with elevated iron levels in the hemolymph, Tsf1 mutants exhibited increased susceptibility to Pseudomonas bacteria and Mucorales fungi, which could be rescued by chemical chelation of iron. Furthermore, using siderophore-deficient Pseudomonas aeruginosa, we discover that the siderophore pyoverdine is necessary for pathogenesis in wild-type flies, but it becomes dispensable in Tsf1 mutants due to excessive iron present in the hemolymph of these flies. As such, our study reveals that, similar to mammals, Drosophila uses iron limitation as an immune defense mechanism mediated by conserved iron-transporting proteins transferrins. Our in vivo work, together with accumulating in vitro studies, supports the immune role of insect transferrins against infections via an iron withholding strategy.

Assessing potential harmful effects of CdSe quantum dots by using Drosophila melanogaster as in vivo model

2015 ◽  
Vol 530-531 ◽  
pp. 66-75 ◽  
Author(s):  
Mohamed Alaraby ◽  
Esref Demir ◽  
Alba Hernández ◽  
Ricard Marcos
Keyword(s):  
Quantum Dots ◽  
Drosophila Melanogaster ◽  
In Vivo Model ◽  
Cdse Quantum Dots ◽  
Harmful Effects

scholarly journals Nutraceutical Strategy to Counteract Eye Neurodegeneration and Oxidative Stress in Drosophila melanogaster Fed with High-Sugar Diet

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1197
Author(s):  
Elisabetta Catalani ◽  
Giuseppina Fanelli ◽  
Federica Silvestri ◽  
Agnese Cherubini ◽  
Simona Del Quondam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Antioxidant Properties ◽  
Fruit Fly ◽  
Retinal Cell ◽  
Food Supplementation ◽  
In Vivo Model ◽  
Retinal Neurons ◽  
High Sucrose

Aberrant production of reactive oxygen species (ROS) is a common feature of damaged retinal neurons in diabetic retinopathy, and antioxidants may exert both preventive and therapeutic action. To evaluate the beneficial and antioxidant properties of food supplementation with Lisosan G, a powder of bran and germ of grain (Triticum aestivum) obtained by fermentation with selected lactobacillus and natural yeast strains, we used an in vivo model of hyperglycemia-induced retinal damage, the fruit fly Drosophila melanogaster fed with high-sucrose diet. Lisosan G positively affected the visual system of hyperglycemic flies at structural/functional level, decreased apoptosis, and reactivated protective autophagy at the retina internal network. Also, in high sucrose-fed Drosophila, Lisosan G reduced the levels of brain ROS and retina peroxynitrite. The analysis of oxidative stress-related metabolites suggested 7,8-dihydrofolate, uric acid, dihydroorotate, γ-L-glutamyl-L-cysteine, allantoin, cysteinyl-glycine, and quinolate as key mediators of Lisosan G-induced inhibition of neuronal ROS, along with the upregulation of glutathione system. Of note, Lisosan G may impact oxidative stress and the ensuing retinal cell death, also independently from autophagy, although the autophagy-ROS cross-talk is critical. This study demonstrated that the continuous supplementation with the alimentary integrator Lisosan G exerts a robust and multifaceted antioxidant effect on retinal neurons, thus providing efficacious neuroprotection of hyperglycemic eye.

Role of cerium oxide nanoparticles in improving oxidative stress and developmental delays in Drosophila melanogaster as an in-vivo model for bisphenol a toxicity

Chemosphere ◽  
2021 ◽  
pp. 131363
Author(s):  
Arkajyoti Sarkar ◽  
Tharun Selvam Mahendran ◽  
Aasha Meenakshisundaram ◽  
Rushenka Vashti Christopher ◽  
Pallavi Dan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Bisphenol A ◽  
Cerium Oxide ◽  
Developmental Delays ◽  
Cerium Oxide Nanoparticles ◽  
In Vivo Model ◽  
Oxide Nanoparticles

scholarly journals Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay

Mutagenesis ◽  
2005 ◽  
Vol 20 (4) ◽  
pp. 285-290 ◽  
Author(s):  
Hifzur R. Siddique ◽  
D.Kar Chowdhuri ◽  
D.K. Saxena ◽  
Alok Dhawan
Keyword(s):  
Drosophila Melanogaster ◽  
Comet Assay ◽  
In Vivo Model ◽  
Alkaline Comet Assay
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