DAQ based Impedance Measurement System for Low Cost and Portable Electrical Cell-Substrate Impedance Sensing

2018 ◽  
Vol 12 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Il-Hwan Park ◽  
Yeonhee Hong ◽  
Hee-Sook Jun ◽  
Eou-Sik Cho ◽  
Sungbo Cho
Keyword(s):  
Measurement System ◽  
Low Cost ◽  
Impedance Measurement ◽  
Impedance Sensing ◽  
Cell Substrate

Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications

2021 ◽  
Vol 7 (2) ◽  
pp. 496-499
Author(s):  
Stadler B. Eng. Sebastian ◽  
Herbert Plischke ◽  
Christian Hanshans
Keyword(s):  
Measurement System ◽  
Low Cost ◽  
Measurement Data ◽  
Impedance Measurement ◽  
Microtiter Plate ◽  
Label Free ◽  
Single Chip ◽  
Do It Yourself ◽  
Store And Forward

Abstract Bioimpedance analysis is a label-free and easy approach to obtain information on cellular barrier integrity and cell viability more broadly. In this work, we introduce a small, low-cost, portable in vitro impedance measurement system for studies where a shadow-free exposure of the cells is a requirement. It can be controlled by a user-friendly web interface and can perform measurements automated and autonomously at short intervals. The system can be integrated into an existing IoT network for remote monitoring and indepth analyses. A single-board computer (SBC) serves as the central unit, to control, analyze, store and forward the measurement data from the single-chip impedance analyzer. Various materials and manufacturing methods were used to produce a purpose-built lid on top of a modified 24-well microtiter plate in a “do it yourself” fashion. Furthermore, three different sensor designs were developed utilizing anodic aluminum oxide (AAO) membranes and gold-plated electrodes. Preliminary tests with potassium chloride (KCl) showed first promising results.

Design of a Portable Bioelectrical Impedance Measurement System

2011 ◽  
Vol 365 ◽  
pp. 409-414
Author(s):  
Yi Lu ◽  
Cheng Yu Huo ◽  
Ai Jun He
Keyword(s):  
Biomedical Research ◽  
Measurement System ◽  
Low Cost ◽  
Impedance Measurement ◽  
Bioelectrical Impedance ◽  
Usb Bus ◽  
Measurement Devices ◽  
Digital Demodulation ◽  
Electrode Technique ◽  
Impedance Converter

Bioelectrical impedance measurement is a widely used biomedical detecting technology. However, most traditional bioelectrical impedance measurement devices are bulky, expensive and lack of flexibility. In order to reduce costs, increase flexibility and maintain similar performance, a portable bioelectrical impedance measurement system was designed in this paper. The measurement front, with an ARM based microcontroller and integrated impedance converter chip as its core device, communicated with the host computer via USB bus. Based on the four-electrode technique, DFT digital demodulation and software calibration and compensation algorithm, the system realized its functions. Experimental results indicate that the system can achieve high precision bioelectrical impedance measurement. With the advantage of high integration and low-cost, the system can be widely used in household healthcare, clinics and biomedical research and testing.

scholarly journals Impedimetric characterization of human blood using three-electrode based ECIS devices

2019 ◽  
Vol 3 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Rangadhar Pradhan ◽  
Analava Mitra ◽  
Soumen Das
Keyword(s):  
Whole Blood ◽  
Electrochemical Impedance ◽  
Impedance Measurement ◽  
Frequency Range ◽  
Counter Electrodes ◽  
Impedance Sensing ◽  
Cell Substrate ◽  
Specific Impedance ◽  
Lower Frequency Range

Abstract In this study, three-electrode based electric cell-substrate impedance sensing (ECIS) devices were used to study the electrical properties of blood and its constituents using electrochemical impedance spectroscopy. The three-electrode based ECIS devices were fabricated by using micromachining technology with varying sizes for working, reference and counter electrodes. The blood and its constituents such as serum, plasma, and red blood cells (RBCs) were prepared by conventional methods and stored for impedance measurement using fabricated microdevices. Equivalent circuits for blood, serum, plasma and RBCs were proposed using the software package ZSimpWin to validate the experimental data. The proposed equivalent circuit models of blood and its components have excellent agreement up to a frequency of 1 MHz. It is evident from the experimental results that blood and its components have specific impedance signatures that decrease with the increase of frequency. Blood shows higher impedance than the other samples in the lower frequency range (<50 kHz). It was also found that above 50 kHz, the impedance value of RBCs is nearly the same as whole blood. The impedance of serum and plasma steadily decreases with the increase of frequency up to 100 kHz and flattens out after that. The minimum impedance value achieved for serum and plasma is much less than the value obtained for whole blood.

scholarly journals Electric Cell-Substrate Impedance Sensing To Monitor Viral Growth and Study Cellular Responses to Infection with Alphaherpesviruses in Real Time

mSphere ◽  
2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Matthew R. Pennington ◽  
Gerlinde R. Van de Walle
Keyword(s):  
Real Time ◽  
Cellular Response ◽  
Cellular Responses ◽  
Cellular Damage ◽  
Impedance Sensing ◽  
Novel Technologies ◽  
Antiviral Therapies ◽  
Mucosal Surfaces ◽  
Cell Substrate ◽  
Hsv 1

ABSTRACT Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner. Electric cell-substrate impedance sensing (ECIS) measures changes in an electrical circuit formed in a culture dish. As cells grow over a gold electrode, they block the flow of electricity and this is read as an increase in electrical impedance in the circuit. ECIS has previously been used in a variety of applications to study cell growth, migration, and behavior in response to stimuli in real time and without the need for cellular labels. Here, we demonstrate that ECIS is also a valuable tool with which to study infection by alphaherpesviruses. To this end, we used ECIS to study the kinetics of cells infected with felid herpesvirus type 1 (FHV-1), a close relative of the human alphaherpesviruses herpes simplex virus 1 (HSV-1) and HSV-2, and compared the results to those obtained with conventional infectivity assays. First, we demonstrated that ECIS can easily distinguish between wells of cells infected with different amounts of FHV-1 and provides information about the cellular response to infection. Second, we found ECIS useful in identifying differences between the replication kinetics of recombinant DsRed Express2-labeled FHV-1, created via CRISPR/Cas9 genome engineering, and wild-type FHV-1. Finally, we demonstrated that ECIS can accurately determine the half-maximal effective concentration of antivirals. Collectively, our data show that ECIS, in conjunction with current methodologies, is a powerful tool that can be used to monitor viral growth and study the cellular response to alphaherpesvirus infection. IMPORTANCE Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner.

scholarly journals High-Resolution Atmospheric Sensing of Multiple Atmospheric Variables Using the DataHawk Small Airborne Measurement System

2013 ◽  
Vol 30 (10) ◽  
pp. 2352-2366 ◽  
Author(s):  
Dale A. Lawrence ◽  
Ben B. Balsley
Keyword(s):  
High Resolution ◽  
Measurement System ◽  
Current System ◽  
Low Cost ◽  
Structure Constant ◽  
Turbulence Structure ◽  
Quality Of Data ◽  
Airborne Measurement ◽  
Atmospheric Measurement ◽  
Atmospheric Variables

Abstract The DataHawk small airborne measurement system provides in situ atmospheric measurement capabilities for documenting scales as small as 1 m and can access reasonably large volumes in and above the atmospheric boundary layer at low cost. The design of the DataHawk system is described, beginning with the atmospheric measurement requirements, and articulating five key challenges that any practical measurement system must overcome. The resulting characteristics of the airborne and ground support components of the DataHawk system are outlined, along with its deployment, operating, and recovery modes. Typical results are presented to illustrate the types and quality of data provided by the current system, as well as the need for more of these finescale measurements. Particular focus is given to the DataHawk's ability to make very-high-resolution measurements of a variety of atmospheric variables simultaneously, with emphasis given to the measurement of two important finescale turbulence parameters, (the temperature turbulence structure constant) and ɛ (the turbulent energy dissipation rate). Future sensing possibilities and limitations using this approach are also discussed.

Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study

The Analyst ◽  
2011 ◽  
Vol 136 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Jongin Hong ◽  
Karthikeyan Kandasamy ◽  
Mohana Marimuthu ◽  
Cheol Soo Choi ◽  
Sanghyo Kim
Keyword(s):  
Cancer Cell ◽  
Impedance Sensing ◽  
Non Invasive ◽  
Cell Substrate ◽  
Cell Study

scholarly journals Comparison of Leading Biosensor Technologies to Detect Changes in Human Endothelial Barrier Properties in Response to Pro-Inflammatory TNFα and IL1β in Real-Time

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 159
Author(s):  
James J. W. Hucklesby ◽  
Akshata Anchan ◽  
Simon J. O'Carroll ◽  
Charles P. Unsworth ◽  
E. Scott Graham ◽  
...  
Keyword(s):  
Human Brain ◽  
Real Time ◽  
Barrier Properties ◽  
Endothelial Barrier ◽  
Endothelial Monolayer ◽  
Impedance Sensing ◽  
Endothelial Monolayers ◽  
Impedance Data ◽  
Cell Substrate ◽  
Limited Frequency

Electric Cell-Substrate Impedance Sensing (ECIS), xCELLigence and cellZscope are commercially available instruments that measure the impedance of cellular monolayers. Despite widespread use of these systems individually, direct comparisons between these platforms have not been published. To compare these instruments, the responses of human brain endothelial monolayers to TNFα and IL1β were measured on all three platforms simultaneously. All instruments detected transient changes in impedance in response to the cytokines, although the response magnitude varied, with ECIS being the most sensitive. ECIS and cellZscope were also able to attribute responses to particular endothelial barrier components by modelling the multifrequency impedance data acquired by these instruments; in contrast the limited frequency xCELLigence data cannot be modelled. Consistent with its superior impedance sensing, ECIS exhibited a greater capacity than cellZscope to distinguish between subtle changes in modelled endothelial monolayer properties. The reduced resolving ability of the cellZscope platform may be due to its electrode configuration, which is necessary to allow access to the basolateral compartment, an important advantage of this instrument. Collectively, this work demonstrates that instruments must be carefully selected to ensure they are appropriate for the experimental questions being asked when assessing endothelial barrier properties.

scholarly journals Visual Measurement System for Wheel–Rail Lateral Position Evaluation

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1297
Author(s):  
Viktor Skrickij ◽  
Eldar Šabanovič ◽  
Dachuan Shi ◽  
Stefano Ricci ◽  
Luca Rizzetto ◽  
...  
Keyword(s):  
Measurement System ◽  
Inertial Sensor ◽  
Low Cost ◽  
Image Point ◽  
Low Velocity ◽  
Point Selection ◽  
Lateral Direction ◽  
Initial Field ◽  
Visual Measurement ◽  
Track Geometry

Railway infrastructure must meet safety requirements concerning its construction and operation. Track geometry monitoring is one of the most important activities in maintaining the steady technical conditions of rail infrastructure. Commonly, it is performed using complex measurement equipment installed on track-recording coaches. Existing low-cost inertial sensor-based measurement systems provide reliable measurements of track geometry in vertical directions. However, solutions are needed for track geometry parameter measurement in the lateral direction. In this research, the authors developed a visual measurement system for track gauge evaluation. It involves the detection of measurement points and the visual measurement of the distance between them. The accuracy of the visual measurement system was evaluated in the laboratory and showed promising results. The initial field test was performed in the Vilnius railway station yard, driving at low velocity on the straight track section. The results show that the image point selection method developed for selecting the wheel and rail points to measure distance is stable enough for TG measurement. Recommendations for the further improvement of the developed system are presented.

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