Production of rhamnolipids with a high specificity by Pseudomonas aeruginosa M408 isolated from petroleum-contaminated soil using olive oil as sole carbon source

2016 ◽  
Vol 66 (3) ◽  
pp. 1145-1156 ◽  
Author(s):  
Fangling Ji ◽  
Lu Li ◽  
Shuai Ma ◽  
Jingyun Wang ◽  
Yongming Bao
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
Olive Oil ◽  
Contaminated Soil ◽  
Sole Carbon Source ◽  
High Specificity ◽  
Petroleum Contaminated Soil

scholarly journals KARAKTERISTIK BIODEGRADASI ALKIL SULFONAT LINEAR OLEH Pseudomonas aeruginosa I Made Sudiana

2003 ◽  
Vol 9 (1) ◽  
pp. 27-31
Author(s):  
I Made Sudiana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
16S Rdna ◽  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Aerobic Condition ◽  
Toxic Material ◽  
Alkyl Sulfonate ◽  
Human Animal ◽  
Linear Alkyl Sulfonate

Detergent contained of Linear Alkyl Sulfonate (LAS) is toxic material to human, animal and microorganism. Strain S1 isolated from detergent contaminated soil was able to grow in media with LAS as a sole carbon source. LAS degradation took place under aerobic condition, with μmax of 0.31-h, Ks = 7.75 mg/L, Vmax = 1.04 mg/L.hour-1and Km = 8.119 mg/L. Analyses of 16s rDNA revealed that S1 is belonging to Pseudomonas aeruginosa.

Rhamnolipids stabilize quorum sensing mediated cooperation in Pseudomonas aeruginosa

2020 ◽  
Vol 367 (10) ◽  
Author(s):  
Rodolfo García-Contreras ◽  
Daniel Loarca ◽  
Caleb Pérez-González ◽  
J Guillermo Jiménez-Cortés ◽  
Abigail Gonzalez-Valdez ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Carbon Source ◽  
Public Goods ◽  
Sole Carbon Source ◽  
Regulatory Genes ◽  
Chronic Infections ◽  
Toxic Metabolites ◽  
Serial Cultivation ◽  
Selection Of

ABSTRACT Pseudomonas aeruginosa is one of the main models to study social behaviors in bacteria since it synthesizes several exoproducts, including exoproteases and siderophores and release them to the environment. Exoproteases and siderophores are public goods that can be utilized by the individuals that produce them but also by non-producers, that are considered social cheaters. Molecularly exoprotease cheaters are mutants in regulatory genes such as lasR, and are commonly isolated from chronic infections and selected in the laboratory upon serial cultivation in media with protein as a sole carbon source. Despite that the production of exoproteases is exploitable, cooperators have also ways to restrict the growth and selection of social cheaters, for instance by producing toxic metabolites like pyocyanin. In this work, using bacterial competitions, serial cultivation and growth assays, we demonstrated that rhamnolipids which production is regulated by quorum sensing, selectively affect the growth of lasR mutants and are able to restrict social cheating, hence contributing to the maintenance of cooperation in Pseudomonas aeruginosa populations.

Antigenic variation and increase in pathogenicity in Pseudomonas aeruginosa as a result of growth on glucose or N-hexadecane as sole carbon source

1978 ◽  
Vol 24 (6) ◽  
pp. 675-679 ◽  
Author(s):  
Robert S. Stinson ◽  
D. E. Talburt
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
Outer Membrane ◽  
Sole Carbon Source ◽  
Antigenic Variation ◽  
Protein Composition ◽  
Cell Types ◽  
Additional Protein

When Pseudomonas aeruginosa is grown on glucose as opposed to n-hexadecane as the sole carbon source, the antigenicity, virulence, and protein composition of the outer membrane are altered. The hydrocarbon-grown cells demonstrate a 3-log increase in virulence over the glucose-grown cells (in mice). There also appears to be an additional protein present in the outer membrane of the n-hexadecane-grown cells. This protein may contribute to the observed antigenic differences between the two cell types.

Laboratory scale bioremediation of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet

2010 ◽  
Vol 101 (16) ◽  
pp. 6545-6552 ◽  
Author(s):  
A.K. Karamalidis ◽  
A.C. Evangelou ◽  
E. Karabika ◽  
A.I. Koukkou ◽  
C. Drainas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soil ◽  
Laboratory Scale ◽  
Indigenous Microorganisms ◽  
Petroleum Contaminated Soil

Bioremediation of Petroleum Contaminated Soil

2012 ◽  
Vol 550-553 ◽  
pp. 1248-1252
Author(s):  
Rui Dan Xu
Keyword(s):  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Oil Content ◽  
Degradation Rate ◽  
Low Cost ◽  
Oil Fields ◽  
Degrading Bacteria ◽  
Petroleum Contaminated Soil ◽  
Immobilized Microorganisms ◽  
Degradation Ability

Two kinds of polyacrylamide(HPAM)-degrading bacteria S1, S2, which can use HPAM as only nitrogen source and the sole carbon source, were isolated from petroleum-contaminated soil of Daqing Oilfield. The bioremediation for treating petroleum contaminated soil by immobilized microorganisms can improve the effect on biodegradation for pollutants in oil fields and reduce the loss of bacteria. The degradation ability of five kinds of embedding immobilization methods on soil pollutant was investigated. The experimental results showed that the immobilized microbial granules, which used polyvinyl alcohols (PVA) and sodium alginate as coagulant, activated carbon as coagulant-support, exhibited good mechanical strength, operated easily, be not breakable and low cost. Experiments results showed that after treatment using this kind of immobilized microbial granules, the HPAM concentration declined from 500 mg•L-1 to 102 mg•L-1 in 48 hours. The degradation rate of HPAM reached 79.6%. At the same time crude oil content decreased from 733.21 mg•L-1 to 9.5 mg•L-1. These immobilized microbial granules can remove 98.7% oil from the petroleum-contaminated soil in 48 hours.

scholarly journals Utilization of Crude Glycerol as a Substrate for the Production of Rhamnolipid by Pseudomonas aeruginosa

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Walaa A. Eraqi ◽  
Aymen S. Yassin ◽  
Amal E. Ali ◽  
Magdy A. Amin
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
Sodium Nitrate ◽  
Sole Carbon Source ◽  
Crude Glycerol ◽  
Screening Program ◽  
Waste Glycerol ◽  
Pharmaceutical Industries ◽  
Microbial Isolates ◽  
Biodiesel Industry

Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We have developed a process for the production of rhamnolipid biosurfactants using glycerol as the sole carbon source by a local Pseudomonas aeruginosa isolate that was obtained from an extensive screening program. A factorial design was applied with the goal of optimizing the rhamnolipid production. The highest production yield was obtained after 2 days when cells were grown in minimal salt media at pH 6, containing 1% (v/v) glycerol and 2% (w/v) sodium nitrate as nitrogen source, at 37°C and at 180 rpm, and reached 2.164 g/L after 54 hours (0.04 g/L h). Analysis of the produced rhamnolipids by TLC, HPLC, and FTIR confirmed the nature of the biosurfactant as monorhamnolipid. Glycerol can serve as a source for the production of rhamnolipid from microbial isolates providing a cheap and reliable substrate.

Nitrogen-fixing bacteria capable of utilising kerosene hydrocarbons as a sole carbon source

2000 ◽  
Vol 42 (5-6) ◽  
pp. 407-410 ◽  
Author(s):  
J. Pérez-Vargas ◽  
H.M. Poggi-Varaldo ◽  
G. Calva-Calva ◽  
E. Ríos-Leal ◽  
R. Rodríguez-Vázquez ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Nitrogenase Activity ◽  
Soil Bioremediation ◽  
Atmospheric Nitrogen ◽  
Nitrogen Fixing ◽  
Aerobic Conditions ◽  
Bioremediation Processes

Several nitrogen fixing consortia (NFC) were isolated from kerosene contaminated soil, where Phaseolus vulgaris plants were being cultivated. The capability of these consortia for kerosene hydrocarbons removal was investigated and demonstrated. The NFC cultivated under aerobic conditions, and kerosene as sole carbon source, effected a maximum of 75% of reduction of the total kerosene hydrocarbons. Also, from experiments conducted to evaluate their atmospheric nitrogen fixing capability, all consortia showed nitrogenase activity: from 4 to 183 nmol N2/3E09 bacteria.day. To our knowledge, this is the first report that shows a group of bacteria with the dual characteristic of fixing atmospheric nitrogen and capability to use kerosene hydrocarbons as a sole carbon source. Application of these microorganisms to soil bioremediation processes is discussed.

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