scholarly journals KARAKTERISTIK BIODEGRADASI ALKIL SULFONAT LINEAR OLEH Pseudomonas aeruginosa I Made Sudiana

2003 ◽  
Vol 9 (1) ◽  
pp. 27-31
Author(s):  
I Made Sudiana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
16S Rdna ◽  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Aerobic Condition ◽  
Toxic Material ◽  
Alkyl Sulfonate ◽  
Human Animal ◽  
Linear Alkyl Sulfonate

Detergent contained of Linear Alkyl Sulfonate (LAS) is toxic material to human, animal and microorganism. Strain S1 isolated from detergent contaminated soil was able to grow in media with LAS as a sole carbon source. LAS degradation took place under aerobic condition, with μmax of 0.31-h, Ks = 7.75 mg/L, Vmax = 1.04 mg/L.hour-1and Km = 8.119 mg/L. Analyses of 16s rDNA revealed that S1 is belonging to Pseudomonas aeruginosa.

Rhamnolipids stabilize quorum sensing mediated cooperation in Pseudomonas aeruginosa

2020 ◽  
Vol 367 (10) ◽  
Author(s):  
Rodolfo García-Contreras ◽  
Daniel Loarca ◽  
Caleb Pérez-González ◽  
J Guillermo Jiménez-Cortés ◽  
Abigail Gonzalez-Valdez ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Carbon Source ◽  
Public Goods ◽  
Sole Carbon Source ◽  
Regulatory Genes ◽  
Chronic Infections ◽  
Toxic Metabolites ◽  
Serial Cultivation ◽  
Selection Of

ABSTRACT Pseudomonas aeruginosa is one of the main models to study social behaviors in bacteria since it synthesizes several exoproducts, including exoproteases and siderophores and release them to the environment. Exoproteases and siderophores are public goods that can be utilized by the individuals that produce them but also by non-producers, that are considered social cheaters. Molecularly exoprotease cheaters are mutants in regulatory genes such as lasR, and are commonly isolated from chronic infections and selected in the laboratory upon serial cultivation in media with protein as a sole carbon source. Despite that the production of exoproteases is exploitable, cooperators have also ways to restrict the growth and selection of social cheaters, for instance by producing toxic metabolites like pyocyanin. In this work, using bacterial competitions, serial cultivation and growth assays, we demonstrated that rhamnolipids which production is regulated by quorum sensing, selectively affect the growth of lasR mutants and are able to restrict social cheating, hence contributing to the maintenance of cooperation in Pseudomonas aeruginosa populations.

Antigenic variation and increase in pathogenicity in Pseudomonas aeruginosa as a result of growth on glucose or N-hexadecane as sole carbon source

1978 ◽  
Vol 24 (6) ◽  
pp. 675-679 ◽  
Author(s):  
Robert S. Stinson ◽  
D. E. Talburt
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
Outer Membrane ◽  
Sole Carbon Source ◽  
Antigenic Variation ◽  
Protein Composition ◽  
Cell Types ◽  
Additional Protein

When Pseudomonas aeruginosa is grown on glucose as opposed to n-hexadecane as the sole carbon source, the antigenicity, virulence, and protein composition of the outer membrane are altered. The hydrocarbon-grown cells demonstrate a 3-log increase in virulence over the glucose-grown cells (in mice). There also appears to be an additional protein present in the outer membrane of the n-hexadecane-grown cells. This protein may contribute to the observed antigenic differences between the two cell types.

scholarly journals Utilization of Crude Glycerol as a Substrate for the Production of Rhamnolipid by Pseudomonas aeruginosa

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Walaa A. Eraqi ◽  
Aymen S. Yassin ◽  
Amal E. Ali ◽  
Magdy A. Amin
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Source ◽  
Sodium Nitrate ◽  
Sole Carbon Source ◽  
Crude Glycerol ◽  
Screening Program ◽  
Waste Glycerol ◽  
Pharmaceutical Industries ◽  
Microbial Isolates ◽  
Biodiesel Industry

Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We have developed a process for the production of rhamnolipid biosurfactants using glycerol as the sole carbon source by a local Pseudomonas aeruginosa isolate that was obtained from an extensive screening program. A factorial design was applied with the goal of optimizing the rhamnolipid production. The highest production yield was obtained after 2 days when cells were grown in minimal salt media at pH 6, containing 1% (v/v) glycerol and 2% (w/v) sodium nitrate as nitrogen source, at 37°C and at 180 rpm, and reached 2.164 g/L after 54 hours (0.04 g/L h). Analysis of the produced rhamnolipids by TLC, HPLC, and FTIR confirmed the nature of the biosurfactant as monorhamnolipid. Glycerol can serve as a source for the production of rhamnolipid from microbial isolates providing a cheap and reliable substrate.

Nitrogen-fixing bacteria capable of utilising kerosene hydrocarbons as a sole carbon source

2000 ◽  
Vol 42 (5-6) ◽  
pp. 407-410 ◽  
Author(s):  
J. Pérez-Vargas ◽  
H.M. Poggi-Varaldo ◽  
G. Calva-Calva ◽  
E. Ríos-Leal ◽  
R. Rodríguez-Vázquez ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Nitrogenase Activity ◽  
Soil Bioremediation ◽  
Atmospheric Nitrogen ◽  
Nitrogen Fixing ◽  
Aerobic Conditions ◽  
Bioremediation Processes

Several nitrogen fixing consortia (NFC) were isolated from kerosene contaminated soil, where Phaseolus vulgaris plants were being cultivated. The capability of these consortia for kerosene hydrocarbons removal was investigated and demonstrated. The NFC cultivated under aerobic conditions, and kerosene as sole carbon source, effected a maximum of 75% of reduction of the total kerosene hydrocarbons. Also, from experiments conducted to evaluate their atmospheric nitrogen fixing capability, all consortia showed nitrogenase activity: from 4 to 183 nmol N2/3E09 bacteria.day. To our knowledge, this is the first report that shows a group of bacteria with the dual characteristic of fixing atmospheric nitrogen and capability to use kerosene hydrocarbons as a sole carbon source. Application of these microorganisms to soil bioremediation processes is discussed.

scholarly journals Fluorescent pseudomonads capable of growth at 41 degrees C but distinct from Pseudomonas aeruginosa

1976 ◽  
Vol 4 (5) ◽  
pp. 443-449
Author(s):  
G W Ajello ◽  
A W Hoadley
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid ◽  
Carbon Source ◽  
Antibiotic Susceptibility ◽  
Sole Carbon Source ◽  
Cellular Fatty Acid ◽  
Fluorescent Pseudomonads ◽  
Nitrogen Gas ◽  
Fluorescent Pseudomonas ◽  
Fatty Acid Compositions

One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results were compared with those from 40 apyocyanogenic and 14 pyocyanin-producing strains of P. aeruginosa. Unidentified fluorescent Pseudomonas (UFP) strains generally were not agglutinated by P. aeruginosa antisera and showed little or no pyocin activity. In contrast to P. aeruginosa strains, UFP strains usually failed to oxidize D-gluconate or reduce nitrate to nitrogen gas. They could not use D-gluconate or D-mannitol as sole carbon source and were susceptible to kanamycin. The cellular fatty acid compositions of major UFP groups resembled those of the alcaligenes-stutzeri groups.

Isolation and Mutagenesis of a Novel Phenol-Degrading Strain

2013 ◽  
Vol 647 ◽  
pp. 588-594 ◽  
Author(s):  
Ren Peng ◽  
Gui Juan Yang ◽  
Qi Ming Wang ◽  
Yun Yun Du ◽  
Jia Rong Li
Keyword(s):  
Carbon Source ◽  
16S Rdna ◽  
Sole Carbon Source ◽  
Sole Source ◽  
Morphological Features ◽  
Rhodococcus Ruber ◽  
16S Rdna Sequence ◽  
Rotting Wood ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

In this study, with phenol as sole source of carbon, a phenol-degrading strain was isolated from rotting wood and polluted sludge. The strain was identified as Rhodococcus ruber SD3 according to their morphological features and 16S rDNA sequence. Rhodococcus ruber SD3 almost completely degraded 1.0g L-1 phenol in 72 hours. Rhodococcus ruber SD3 was also capable of growing in a medium containing isooctane, cyclohexane, benzene, n-heptane, toluene, acetonitrile, chlorobenzene, naphthalene, n-hexane, 1-naphthol and dimethylbenzene as sole carbon source, respectively. Rhodococcus ruber SD3 was mutated using LiCl as a chemical mutagen. The optimal concentration of LiCl for mutagenesis was 0.3 %. The mutant M1 could degrade 99.8 % of 1.5 g L-1 phenol within 72h. The optimum pH and temperature for the degradation of phenol with mutant M1 were 7.5 and 35°C.

Isolation and Characterization of a Styrene Trimer Degrading Microorganism

2014 ◽  
Vol 1073-1076 ◽  
pp. 666-671
Author(s):  
Guang Chun Li ◽  
Chun Xiang Piao ◽  
Katsuhiko Saido ◽  
Seon Yong Chung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Liquid Phase ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Ochrobactrum Intermedium ◽  
Complex Strain ◽  
Degrading Microorganism

Biodegradation of the styrene trimer was investigated, and its degrading bacteria were screened and isolated. Complex bacteria ST (strain ST1 and ST2) was isolated from contaminated soil by polystyrene and named by strain ST1 and ST2. ST1 and ST2 were identified by 16S rDNA and classified byOchrobactrum intermediumsp. andPseudomonas aeruginosasp., respectively. Biodegradation experiments were performed in batch and styrene trimer was used as a sole carbon source. Isolated two bacteria were used as degrading microorganisms. Initial liquid phase concentration of the styrene trimer was 50 mg/L. 95% of the styrene trimer was degraded in 17 days by the complex strain ST. The concentration was analyzed by using GC. Metabolites of bacteria were analyzed and three kinds of products that were identified by GC/MS.

scholarly journals BIODEGRADASI LIMBAH OLI BEKAS OLEH Lycinibacillus sphaericus TCP C 2.1

2016 ◽  
Vol 12 (2) ◽  
pp. 111
Author(s):  
Witono Basuki
Keyword(s):  
Carbon Source ◽  
Soil Sample ◽  
16S Rdna ◽  
Sole Carbon Source ◽  
Engine Oil ◽  
Used Engine Oil ◽  
Almost All

Mikroorganism has capability to degrade used engine oil was isolated from soil sample contaminated with used engine oil. One of the selected strain TCP C 2.1 was identified by 16s rDNA as Lycinibacillus sphaericus. The microorganism can use hydrocarbon in used engine oil as the sole carbon source and energy, also it significantly degraded almost all hydrocarbon compounds in used engine oil. With its ability the microorganism has potency to be used as a single microbe for bioremediation of soil polluted by engine oil.

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