Bioremediation of Petroleum Contaminated Soil

2012 ◽  
Vol 550-553 ◽  
pp. 1248-1252
Author(s):  
Rui Dan Xu
Keyword(s):  
Contaminated Soil ◽  
Sole Carbon Source ◽  
Oil Content ◽  
Degradation Rate ◽  
Low Cost ◽  
Oil Fields ◽  
Degrading Bacteria ◽  
Petroleum Contaminated Soil ◽  
Immobilized Microorganisms ◽  
Degradation Ability

Two kinds of polyacrylamide(HPAM)-degrading bacteria S1, S2, which can use HPAM as only nitrogen source and the sole carbon source, were isolated from petroleum-contaminated soil of Daqing Oilfield. The bioremediation for treating petroleum contaminated soil by immobilized microorganisms can improve the effect on biodegradation for pollutants in oil fields and reduce the loss of bacteria. The degradation ability of five kinds of embedding immobilization methods on soil pollutant was investigated. The experimental results showed that the immobilized microbial granules, which used polyvinyl alcohols (PVA) and sodium alginate as coagulant, activated carbon as coagulant-support, exhibited good mechanical strength, operated easily, be not breakable and low cost. Experiments results showed that after treatment using this kind of immobilized microbial granules, the HPAM concentration declined from 500 mg•L-1 to 102 mg•L-1 in 48 hours. The degradation rate of HPAM reached 79.6%. At the same time crude oil content decreased from 733.21 mg•L-1 to 9.5 mg•L-1. These immobilized microbial granules can remove 98.7% oil from the petroleum-contaminated soil in 48 hours.

Experimental Study on Bioremediation of Petroleum Contaminated Soil in Puyang Oilfield

2011 ◽  
Vol 233-235 ◽  
pp. 693-696
Author(s):  
Li Chen ◽  
Fa Wang Zhang ◽  
Shuo Ren ◽  
Sheng Zhang
Keyword(s):  
Experimental Study ◽  
Nitrogen Fixation ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Soil Layer ◽  
Water Oxygen ◽  
Petroleum Contaminated Soil ◽  
Ecological Remediation ◽  
Distinct Increase

According to the previous experience on remediation experiment in laboratory and field, the study on microgial ecological remediation of petroleum contaminated soil in Puyang oilfield was carried out under 3 different conditions. Here the results from remediation show that the technology of microbe cooperated with alfalfa, single microbe technology and single alfalfa technology all possess remedial effect on petroleum contaminated soil through 99-day period remediation, and the technology of microbe cooperated with alfalfa creates the best remedial mission with petroleum hydrocarbon degradation rate of 65.27%, while the rest single technologies exerts degradation rate of about 40% on petroleum hydrocarbon, Moreover, the experiment results indicate that few nutriment such as soluble salt, NO3-, Cl-,etc can infiltrate into the lower soil layer(50cm). However, the distinct increase of NH4+ in the second and third remediation area may attribute to abundance fertilizer transportation into depth soil layer due to the nitrogen fixation of alfalfa’s roots. In addition, we also find that the remediation effect can be impacted by the factors containing tempertation, water, oxygen, nutriment and mini-geo-enviroment.

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

Study on the Optimum Degradation Conditions of Combination Remediation on Petroleum-Contaminated Soil by Cold-Adapted Bacterial and Winter Wheat

2012 ◽  
Vol 209-211 ◽  
pp. 1087-1092
Author(s):  
Xin Zhang ◽  
Hong Qi Wang ◽  
Qian Wang ◽  
Ya Fu Zhang
Keyword(s):  
Winter Wheat ◽  
Petroleum Hydrocarbons ◽  
Oil Content ◽  
Degradation Rate ◽  
Orthogonal Experiment ◽  
Test Method ◽  
Cold Adapted ◽  
Orthogonal Test Method ◽  
Petroleum Contaminated Soil ◽  
The Impact

Through greenhouse pots experiments, an orthogonal test method was employed here to study the optimum degradation conditions of cold-adapted degrading bacterial and the winter wheat. This orthogonal experiment selected four influencing factors: oil content, bacteria amount, soil moisture content and fertilizer. The results showed that in different stages, the optimum degradation conditions were different. Oil content and the bacterial amount were the two most important factors that influenced the degradation of petroleum hydrocarbons. The impact of fertilizers on the degradation rate increased with time, while water content on the degradation rate decreased with time.

Biodegradation of Swainsonine by Stenotrophomonas maltophilia Strain YLZZ-2 and its Isolation and Identification

2010 ◽  
Vol 178 ◽  
pp. 59-64
Author(s):  
Xing Hua Zhao ◽  
Xin He ◽  
Jian Hua Wang
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Degradation Rate ◽  
Ph Value ◽  
Culture Conditions ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
Degrading Bacteria ◽  
Initial Ph ◽  
Optimal Ph

Eight swainsonine (SW)-degrading bacteria were isolated from the soil where locoweed was buried for 6 months and one of the strains (YLZZ-2) was selected for further study. Based on morphology, physiologic tests, 16S rDNA sequence, and phylogenetic characteristics, the strain showed the greatest similarity to members of the order Stenotrophomonap and the closest to members of the Stenotrophomonas maltophilia group. The ability of the strain to degrade SW, as sole carbon source, was investigated under different culture conditions. The preferential temperature and initial pH value for the strain were 25～35 °C and 6.0～9.0, respectively. The optimal temperature for the strain was 30 °C and the optimal pH value was 7.0. There was positive correlation between degradation rate and inoculation amount. The growth of stain YLZZ-2 and degradation rate were fast, and YLZZ-2 could completely degradate 400 mg/L swainsoine within 24 h. There was a linear relationship between the growth of stain YLZZ-2 and degradation of swainsonine. These results highlight the potential of this bacterium to be used in detoxifying of SW in livestock consuming locoweed.

scholarly journals BIOREMEDIATION IN PETROLEUM CONTAMINATED SOIL TREATMENT USING PLANT-MICROORGANISMS COMBINATION (Case Study: Reduction Level of TPH and BTEX in Bioremediation Process)

2016 ◽  
Vol 39 (1) ◽  
pp. 41-51
Author(s):  
Cut Nanda Sari ◽  
Tyas Putri Sativa ◽  
Setyo Sarwanto Moersidik
Keyword(s):  
Contaminated Soil ◽  
Oil Content ◽  
Oil Spills ◽  
Ph Value ◽  
Statistical Tests ◽  
Soil Treatment ◽  
Test Results ◽  
Terrestrial Environments ◽  
Petroleum Contaminated Soil

Oil spills, in both aquatic and terrestrial environments, are very detrimental to people and the environment due to hydrocarbon compounds that are contained in oil which are not only be harmful for the balance of the ecosystem and the environment but also carcinogenic to humans and animals. Therefore remediation needs to be done. One of the methods is by using a combination of microorganisms and plants. The aim of this research is to analyze the in􀃀 uences between several different treatments that are applied for TPH and BTEX removal in the process of remediation. In this research, bioremediation was conducted by using four different treatments which are: by adding compost (C), plants and compost (P), microorganisms and compost (B), and compost, plants and microorganisms (BP), to soil with oil content of 5% and 10%. The following test results of TPH in soil contaminated with 5% oil content are: 2.10% (C); 1.31% (B); 1.66% (P); and 0.68% (BP). The TPH test results in soil contaminated with oil content of 10% are: 3.30% (C); 2.54 (B); 3.91% (P); and 3.31% (BP). The highest percentage of TPH degradation in contaminated soil of 5% oil content was found in BP treatment (87.1%), while in the contaminated soil of 10% oil content the largest TPH removal percentage is by the treatment of adding bacteria (B) which is 76.19%. BTEX removal percentage in 5% oil contaminated soil in BP treatment is 68.35% while in 10% oil contaminated soil with B treatment the removal percentage is 84.91%. Based on statistical tests, both on contaminated soil with 5% and 10% oil content, TPH degradation signi􀂿 cantly affects the pH value as p 0.05 but TPH degradation does not affect temperature values as p 0.05.

scholarly journals Bacteria-white-rot fungi joint remediation of petroleum-contaminated soil based on sustained-release of laccase

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 39075-39081 ◽  
Author(s):  
Boqun Liu ◽  
Jinpeng Liu ◽  
Meiting Ju ◽  
Xiaojing Li ◽  
Ping Wang
Keyword(s):  
Sustained Release ◽  
Contaminated Soil ◽  
White Rot Fungi ◽  
White Rot ◽  
Petroleum Contaminated Soil ◽  
Bacteria And Fungi ◽  
Degradation Ability

This research adopted a new way for white-rot fungi to play a full part in the degradation ability of both bacteria and fungi.

scholarly journals Characterisation of the rhizoremediation of petroleum-contaminated soil: effect of different influencing factors

2010 ◽  
Vol 7 (12) ◽  
pp. 3961-3969 ◽  
Author(s):  
J. C. Tang ◽  
R. G. Wang ◽  
X. W. Niu ◽  
M. Wang ◽  
H. R. Chu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Influencing Factors ◽  
Plant Species ◽  
Tall Fescue ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Degradation Rate ◽  
Initial Period ◽  
Continuous Increase ◽  
Petroleum Contaminated Soil

Abstract. Pilot experiments were conducted to analyse the effect of different environmental factors on the rhizoremediation of petroleum-contaminated soil. Different plant species (cotton, ryegrass, tall fescue and alfalfa), the addition of fertilizer, different concentrations of total petroleum hydrocarbons (TPH) in the soil, bioaugmentation with effective microbial agents (EMA) and plant growth-promoting rhizobacteria (PGPR) and remediation time were tested as influencing factors during the bioremediation process of TPH. The results show that the remediation process can be enhanced by different plant species. The order of effectiveness of the plants was the following: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition, and a moderate urea level of 20 g N (Nitrogen)/m2 was best for both plant growth and TPH remediation. A high TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbons. The results showed that a 5% TPH content gave the best degradation in soil planted with ryegrass. Bioaugmentation with different bacteria and PGPR yielded the following results for TPH degradation: cotton+EMA+PGPR > cotton+EMA > cotton+PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms. A continuous increase of degradation rate was found during the 30–90 days period followed by a slow increase during the 90–150 days period. These results suggest that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

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