Characterization of osmophore activity and secondary metabolite localization in fragrant ephemeral flowers of Murraya paniculata (Linn.) Jack (Rutaceae)

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

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Chemical Components and Bioactive of Gorgonian Sea Plumes (Genus Rumphella and Hicksonella) from Raijua Islands-East Nusa Tenggara

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v18i1.9566 ◽

2015 ◽

Vol 18 (1) ◽

Author(s):

Yunialdi Happynes Teffu ◽

Ruddy Suwandi ◽

Nurjanah Nurjanah

Keyword(s):

Heavy Metal ◽

Secondary Metabolite ◽

Bioactive Compounds ◽

Proximate Analysis ◽

Chemical Components ◽

Metal Analysis ◽

Pharmaceutical Ingredients ◽

Quality Testing ◽

Protein Components

Sea plumes is mostly used by people as jewellery and arthritis drug. The aim of this research was to characterize the chemicals and the bioactive compounds of the sea plumes for pharmaceutical ingredients. Characterization of sea plumes was carried out schlerit form identification, proximate analysis, heavy metal analysis using Atomic Absorption Spectrofotometer, radium content using gamma spectrometer, extraction by maceration method for 24 hour and phytochemicals quality testing based on color appearance. The sea plumes was identified as Rumphella and Hicksonella contains the highest protein components. Heavy metal Pb exceed the standar set to National Standar of Indonesia (SNI) and Medicines and Foods Security Agency. Akar bahar containing radium 0.65-0.79 Becquerel/kilogram, highest yield using methanol and containing secondary metabolite which targeted as bioactive compounds alkaloids, flavonoids, phenols hydroquinone, steroids, triterpenoids and saponins. Keywords : Hicksonella, secondary metabolite, radium, Rumphella, sklerit

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Molecular characterization of the PR-toxin gene cluster in Penicillium roqueforti and Penicillium chrysogenum: Cross talk of secondary metabolite pathways

Fungal Genetics and Biology ◽

10.1016/j.fgb.2013.10.009 ◽

2014 ◽

Vol 62 ◽

pp. 11-24 ◽

Cited By ~ 39

Author(s):

Pedro I. Hidalgo ◽

Ricardo V. Ullán ◽

Silvia M. Albillos ◽

Olimpio Montero ◽

María Ángeles Fernández-Bodega ◽

...

Keyword(s):

Gene Cluster ◽

Molecular Characterization ◽

Secondary Metabolite ◽

Cross Talk ◽

Penicillium Chrysogenum ◽

Toxin Gene ◽

Penicillium Roqueforti

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Erratum to: Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity

Journal of the Korean Society for Applied Biological Chemistry ◽

10.1007/s13765-015-0067-1 ◽

2015 ◽

Vol 58 (6) ◽

pp. 955-955

Author(s):

M. I. R. Mamun ◽

A. M. Abd El-Aty ◽

Md. Musfiqur Rahman ◽

Jeong-Heui Choi ◽

Kyeong Won Yun ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Secondary Metabolite ◽

Gas Chromatography Mass Spectrometry ◽

Sample Injection ◽

Chromatography Mass Spectrometry ◽

Artemisia Princeps ◽

Direct Sample

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Identification and Characterization of the Asperthecin Gene Cluster of Aspergillus nidulans

Applied and Environmental Microbiology ◽

10.1128/aem.01743-08 ◽

2008 ◽

Vol 74 (24) ◽

pp. 7607-7612 ◽

Cited By ~ 116

Author(s):

Edyta Szewczyk ◽

Yi-Ming Chiang ◽

C. Elizabeth Oakley ◽

Ashley D. Davidson ◽

Clay C. C. Wang ◽

...

Keyword(s):

Secondary Metabolite ◽

Polyketide Synthase ◽

Gene Clusters ◽

Laboratory Culture ◽

Culture Conditions ◽

Type I ◽

Genes Encoding ◽

Identification And Characterization ◽

Normal Laboratory

ABSTRACT The sequencing of Aspergillus genomes has revealed that the products of a large number of secondary metabolism pathways have not yet been identified. This is probably because many secondary metabolite gene clusters are not expressed under normal laboratory culture conditions. It is, therefore, important to discover conditions or regulatory factors that can induce the expression of these genes. We report that the deletion of sumO, the gene that encodes the small ubiquitin-like protein SUMO in A. nidulans, caused a dramatic increase in the production of the secondary metabolite asperthecin and a decrease in the synthesis of austinol/dehydroaustinol and sterigmatocystin. The overproduction of asperthecin in the sumO deletion mutant has allowed us, through a series of targeted deletions, to identify the genes required for asperthecin synthesis. The asperthecin biosynthesis genes are clustered and include genes encoding an iterative type I polyketide synthase, a hydrolase, and a monooxygenase. The identification of these genes allows us to propose a biosynthetic pathway for asperthecin.

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