Characterization of salivary components separated by paper electrophoresis

1961 ◽  
Vol 3 (2) ◽  
pp. 77-85 ◽  
Author(s):  
I.D. Mandel ◽  
S.A. Ellison
Keyword(s):  
Paper Electrophoresis

Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

Applications of high-voltage paper electrophoresis for the characterization of drug metabolites

Xenobiotica ◽  
1981 ◽  
Vol 11 (12) ◽  
pp. 841-847 ◽  
Author(s):  
J. A. Bell ◽  
A. Bradbury ◽  
L. E. Martin ◽  
R. J. N. Tanner
Keyword(s):  
High Voltage ◽  
Paper Electrophoresis ◽  
Drug Metabolites

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

"U" Protein and Migration Ratios in Paper Electrophoresis

1957 ◽  
Vol 3 (5) ◽  
pp. 599-608 ◽  
Author(s):  
Joel R Stern ◽  
Roland F Mais ◽  
Joseph D Boggs
Keyword(s):  
Protein Complexes ◽  
Periodic Acid ◽  
Paper Electrophoresis ◽  
Evidence Based ◽  
Physiological Conditions ◽  
Sudan Black B ◽  
Carbohydrate Complex ◽  
Liver And Kidney ◽  
And Migration

Abstract Paper electrophoresis of serum from children with liver and kidney disease has led to recognition of a protein which migrates between the 2 and globulins. This protein has been observed by other workers, but because of confusion in nomenclature, a new designation, "U" protein, has been employed. The use of migration ratios in locating peaks of the 2 "U" protein, and globulin when these components resolve poorly has been discussed. Characterization of "U" protein by employing the periodic acid-reduced fuchsin test for carbohydrate-protein complexes and Sudan black B for lipoprotein showed: (1)"U" protein varies in its content of protein-carbohydrate complex, and (2)"U" protein may be lipo-protein. Evidence based on benzidine tests and intentional hemolysis indicated that "U" protein was not an artifact resulting from accidental hemolysis, but a protein which is found in serum under certain physiological conditions.

scholarly journals The small-scale isolation and characterization of adrenocorticotrophin

1969 ◽  
Vol 114 (3) ◽  
pp. 519-528 ◽  
Author(s):  
R. O. Hussa ◽  
T. Winnick ◽  
J. Landon
Keyword(s):  
Room Temperature ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Paper Electrophoresis ◽  
Small Scale ◽  
Acid Extraction ◽  
Isolation And Characterization ◽  
Acetic Acid Extraction ◽  
Cellulose Column

Several methods for isolating adrenocorticotrophin from small quantities of porcine and bovine pituitary tissue are compared. Initial extraction of the hormone by an acid–acetone technique was simpler and more efficient than one employing acetic acid extraction and ether precipitation. Subsequent purification procedures utilizing adsorption of the peptide on to oxycellulose realized the highest yields. CM-cellulose-column chromatography followed by Sephadex-gel filtration were suitable final steps for obtaining highly purified adrenocorticotrophin. The purity of the hormone was demonstrated by determining its amino acid composition, C-terminal analysis, polyacrylamide-gel electrophoresis, chymotrypsin digestion and paper electrophoresis and by radioimmunoassay and bioassay. Adrenocorticotrophin was found to be rapidly destroyed in intact and especially in homogenized glands kept at room temperature. At 4° the rate of destruction was less rapid and at −20° losses were minimal.

High-Voltage Paper Electrophoresis for Characterization of Drug Metabolites

1973 ◽  
Vol 62 (11) ◽  
pp. 1810-1817 ◽  
Author(s):  
Walter D. Conway ◽  
Vijay K. Batra ◽  
Alan Abramowitz
Keyword(s):  
High Voltage ◽  
Paper Electrophoresis ◽  
Drug Metabolites

Characterization of Proteins of Human Amniotic Fluid by Means of Paper Electrophoresis

1964 ◽  
Vol 42 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Richard W. Stander ◽  
Cyrus C. McNutt ◽  
David M. Barton ◽  
Charles E. Werts
Keyword(s):  
Amniotic Fluid ◽  
Paper Electrophoresis ◽  
Human Amniotic Fluid

Complexes of Carbohydrates with Metal Cations. XI. Paper Electrophoresis of Polyols in Solutions of Calcium Ions

1979 ◽  
Vol 32 (9) ◽  
pp. 1993 ◽  
Author(s):  
SJ Angyal ◽  
JA Mills
Keyword(s):  
Calcium Ions ◽  
Metal Cations ◽  
Paper Electrophoresis ◽  
Hydroxyl Groups ◽  
Polyhydroxy Compounds ◽  
Electrophoretic Mobilities ◽  
Configuration And Conformation

Paper electrophoresis in an electrolyte containing calcium ions is a method suitable for the separation and characterization of many polyhydroxy compounds. The method is based on the formation ofcomplexes between the cation and at least two hydroxyl groups of the compounds. Electrophoretic mobilities are listed for many sugars, methyl glycosides and cyclitols. The mobilities convey someinformation on the structure of the compounds. The correlation of electrophoretic mobilities with the configuration and conformation of the compounds is discussed.

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