Cultures grown and embedded in situ on Spurr discs, a low viscosity epoxy resin for electron microscopy

1973 ◽  
Vol 80 (2) ◽  
pp. 486-490 ◽  
Author(s):  
Marlene Sabbath ◽  
Barbro Anderson ◽  
H.L. Ioachim
2010 ◽  
Vol 160-162 ◽  
pp. 1405-1408 ◽  
Author(s):  
Qing Ming Jia ◽  
Shao Yun Shan ◽  
Li Hong Jiang ◽  
Ya Ming Wang

Polyaniline(PANI) nanofibers were firstly prepared by rapid mixture method, EP/PANI nanocomposite was obtained by in-situ adding the PANI nanofibers to epoxy resin(EP). Scanning electron microscopy(SEM) proves that the type of doped acids has a little effect on the morphologies of PANI, and the PANI doped by different acids present nanofiber structures with the diameter of about 50 nm. The thermal behaviour of EP/PANI nanocomposites were investigated in detail by using a dynamic rate mode of thermogravimetric analyser (TGA) in inert atmosphere, and the results show that the PANI nanofibers obviously improve thermal stability of pure EP. Compared with EP/PANI doped by nitric acid or sulfuric acid, EP/PANI doped by phosphoric acid has best thermal stability.


Author(s):  
John H. Crowe

Many species of arachnids possess cuticular ridges, the function of which is not known. In the present investigation the structural properties of the ridges in a Trombidiform mite, Panonychus citri were studied, with a view towards discovering the function of the ridges.For scanning electron microscopy the animals were mounted on metal pos s previously coated with conducting silver paint. Best results were obtained by examining live animals, mounted on the posts immediately before examination. For transmission electron microscopy the animals were fixed in cacodylate-buffered glutaraldehyde, post-fixed in osmium, embedded in Spurr's low viscosity epoxy resin, and sectioned on diamond knives.


Author(s):  
John H. Crowe

Several species of marine mites are known to possess elaborate cuticular pores, the function of which is not known (Newell, 1947). In the present investigation the fine structure of the cuticular pores in a Halacarid mite, Copidognathus curtusi was studied, with a view towards discovering the function of the pores.For scanning electron microscopy the animals were fixed in cacodylatebuffered glutaraldehyde, post-fixed in osmium, and freeze-dried. For transmission electron microscopy they were fixed as above, embedded in Spurr's low viscosity epoxy resin, and sectioned on diamond knives.C. curtusi was chosen for study because of the abundance of pores in its cuticle.


1954 ◽  
Vol 27 (4) ◽  
pp. 1047-1050 ◽  
Author(s):  
A. J. de Vries ◽  
V. Ch Dalitz

Abstract The use of preserved concentrated Hevea latex for the production of rubber articles has given birth, during the last decades, to a number of quite new technological methods in rubber manufacture. Starting from an aqueous rubber dispersion of low viscosity as a raw material, instead of the very tough viscoelastic solid rubber, may be advantageous in many respects. Latex technology, however, also presents its particular difficulties and drawbacks, one of which is concerned with the problem of the reinforcement of rubber. Reinforcing fillers like carbon black, which enhance the tear strength and abrasion resistance of masticated rubber, do not exhibit the same beneficial effect on the properties of rubber films cast from latex ; the same is true of all fillers if added in appreciable amounts, with the notable exception of resorcinol-formaldehyde resins formed in situ. Nevertheless, the strength of vulcanized cast films from latex is excellent in comparison with vulcanized sheets of masticated solid rubber. In order to get more insight into the factors which control the rheological and mechanical properties of wet as well as dried and vulcanized latex gels, an extensive study has been undertaken, part of which has been concerned with an elucidation of the structure of these gels by means of electron microscopy. The purpose of this paper is to describe the experimental methods for obtaining electron micrographs and to present some of the results. The rheological and mechanical properties will be discussed in forthcoming publications, one of which has appeared in print already.


Author(s):  
Joseph E. Mazurkiewicz

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.


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