Pathophysiology of single and challenge infections of Haemonchus contortus in Merino sheep: Studies on red cell kinetics and the “self-cure” phenomenon

1975 ◽  
Vol 5 (2) ◽  
pp. 147-157 ◽  
Author(s):  
J.D. Dargie ◽  
E.W. Allonby
Keyword(s):  
Haemonchus Contortus ◽  
Cell Kinetics ◽  
The Self ◽  
Red Cell ◽  
Merino Sheep

Fibrinogen, Platelet and Red Cell Kinetics in Mice Bearing an Experimental Tumor

1975 ◽  
Author(s):  
A. Poggi ◽  
N. Polentarutti ◽  
M. B. Donati ◽  
G. de Gaetano ◽  
S. Garattini
Keyword(s):  
Cell Kinetics ◽  
Fibrinogen Level ◽  
Low Grade ◽  
Red Cell ◽  
Experimental Tumor ◽  
Tumor Implantation ◽  
The Third ◽  
Red Cell Survival ◽  
Observation Period

In view of the possible role of platelets and coagulation mechanisms in the growth and dissemination of solid tumors, a number of haematological parameters have been followed during development of an experimental syngeneic tumor in mice (Lewis Lung Carcinoma, 3LL). This tumor, when transplanted intramuscularly in C57,B1/6 mice, grows locally and gives spontaneous metastases to the lungs. The transplanted animals survive for about 4 weeks. Metastases are visible since the third week. A slight but constant increase in plasma fibrinogen level and a marked thrombocytopenia were observed starting during the second week after tumor implantation. No other significant changes in coagulation and fibrinolysis parameters were found. Moreover, the animals developed a marked haemolytic anaemia, possibly microangiopathic in origin. 125I-fibrinogen survival was decreased of about 20% during the second week after tumor implantation and was not further reduced later on. Fibrinogen turnover was accelerated since the second week and was further increased thereafter, being more than doubled at the end of the third week. Labelled fibrinogen accumulated in the primary tumor and in the lungs; its rats of disappearance from the tumor was much slower than from lungs or blood. These data suggest the occurrence of a low-grade, localized fibrinogen consumption (intravascular coagulation ?). 51Cr-platelet survival was not modified throughout the observation period, whereas platelet turnover was markedly reduced since the end of the second week, suggesting a defective platelet production. 51Cr-red cell survival was drastically reduced to about 30% of controls starting from the second week, whereas labelled red cell turnover was almost doubled. The pathogenetic relevance of the observed modifications in the processes of grwoth and dissemination of 3 LL remains to be established.(Supported by Grant NIH-PHRB-IRO1 CA 12764–01.

The genetic control of red cell glutathione deficiencies in Finnish Landrace and Tasmanian Merino sheep and in crosses between these breeds

1976 ◽  
Vol 87 (2) ◽  
pp. 315-323 ◽  
Author(s):  
Elizabeth M. Tucker ◽  
L. Kilgour ◽  
J. D. Young
Keyword(s):  
Genetic Control ◽  
Autosomal Recessive ◽  
Autosomal Dominant ◽  
Dominant Gene ◽  
Red Cell ◽  
Merino Sheep ◽  
Gene Coding ◽  
Transport Defect ◽  
Low Levels ◽  
Cysteine Synthetase

SummaryFinnish Landrace sheep with low red cell GSH concentrations resulting from a defective transport system for certain arnino acids were crossed with Tasmanian Merino sheep with a red cell GSH deficiency due to impaired activity of the enzyme γ-glutamyl cysteine synthetase. Inheritance data showed that the two types of GSH deficiency were under independent genetic control. In the Finnish Landrace breed, the gene coding for the transport defect (Trn) was inherited as an autosomal recessive and sheep homozygous for this gene had high red cell concentrations of lysine and ornithine (Ly ×) as well as low levels of GSH. In the Tasmanian Merino breed the GSH deficiency behaved as if controlled by an autosomal dominant gene (GSHL). Backcross breeding experiments resulted in lambs which had inherited both types of GSH deficiency. Evidence suggested that such ‘double low’ GSH lambs had an impaired viability. In Tasmanian Merinos the GSH deficiency was established prior to birth. Newborn Finnish Landrace lambs were clearly separable into two types on the basis of their red cell lysine and ornithine content but not on their GSH concentrations.

The Agouti gene: a positional candidate for recessive self-colour pigmentation in Australian Merino sheep

1999 ◽  
Vol 50 (6) ◽  
pp. 1099 ◽  
Author(s):  
Y. M. Parsons ◽  
M. R. Fleet ◽  
D. W. Cooper
Keyword(s):  
Comparative Mapping ◽  
Economic Importance ◽  
The Self ◽  
Positional Candidate ◽  
Chromosome 13 ◽  
Merino Sheep ◽  
Merino Wool ◽  
Agouti Gene ◽  
Mapping Information ◽  
Australian Merino

The occurrence of self-colour pigmentation in the Australian Merino wool flock is of considerable economic importance. The Agouti gene is believed to be responsible for the recessive expression of pigmented fleece. Using comparative mapping information we have investigated the putative homologous ovine map positon of the Agouti gene for linkage to the recessive self-colour phenotype of Australian Merino sheep. Significant results were observed with microsatellites previously mapped to ovine chromosome 13. Comparative data suggest that the ovine Agouti gene would map to the same chromosome, making the Agouti gene a positional candidate for the self-colour phenotype.

scholarly journals Hematopoietic Stem Cell Regulation. II. Chronic Effects of Hypoxic-Hypoxia on CFU Kinetics

Blood ◽  
1973 ◽  
Vol 42 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Brian I. Lord ◽  
Martin J. Murphy
Keyword(s):  
Stem Cell ◽  
Cell Kinetics ◽  
Relative Concentration ◽  
Femoral Shaft ◽  
Red Cell ◽  
Hematopoietic Stem ◽  
Stem Cell Regulation ◽  
Chronic Effects ◽  
Colony Forming Units ◽  
Cell Volumes

Abstract Hypobaric-hypoxia, an established stimulator of erythropoiesis, was used to perturb normal steady-state hematopoiesis and thereby to facilitate an analysis of stem cell kinetics. BDF1 male mice were exposed to a simulated altitude of 22,000 ft for 1-15 days. The following parameters were assessed at daily intervals: packed red cell volumes (PCV), femoral and splenic nucleated cellularity, the relative concentration and total number of colony-forming units (CFU, i.e., stem cells) in the femoral shaft and whole spleen, as well as the turnover of this population of cells using the "3H-thymidine-killing" technique. By 10 days of hypoxia the PCV had risen to 65% and by a fortnight was elevated to, and stabilized at, 70%-75%. After an evanescent increase, the femoral cellularity became slightly hypocellular but returned to normal by 3 days, thence becoming hypercellular from days 5 to 15. The spleen was markedly depleted of cells for 48 hr and recovered to hypercellular levels between 4 and 6 days. It then oscillated from hypercellular to normal levels with a period interval of 5 days. Both femoral and splenic CFU demonstrated oscillations, as did the estimations made on the state of CFU turnover. The femoral and splenic CFU did not decrease in number during the 15 days of hypoxia, indeed, they both revealed cyclic increases. These results are discussed in terms of CFU kinetics and humoral mediation.

Red cell kinetics in thalassaemia intermedia: its use for a prospective prognosis

1985 ◽  
Vol 59 (3) ◽  
pp. 533-539 ◽  
Author(s):  
Y. Najean ◽  
F. Deschryver ◽  
T. Henni ◽  
R. Girot
Keyword(s):  
Cell Kinetics ◽  
Red Cell ◽  
Thalassaemia Intermedia

Serological studies of the self-cure reaction in sheep infected with Haemonchus contortus

1960 ◽  
Vol 11 (4) ◽  
pp. 595 ◽  
Author(s):  
EJL Soulsby ◽  
DF Stewart
Keyword(s):  
Haemonchus Contortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
The Self ◽  
Antigenic Stimulation ◽  
Cure Reaction ◽  
Haemagglutination Test ◽  
The Third ◽  
Agar Diffusion ◽  
Serological Studies

The serological reactions which take place during self-cure of sheep infected with Haemonchus contortus were investigated by complement fixation, haemagglutination, and the Ouchterlony agar diffusion precipitin techniques. In addition, a test to detect incomplete antibodies was applied to certain sera. Absorption tests demonstrated that the antibodies detected by the complement fixation test and the haemagglutination test could be differentiated. Evidence is presented to show that the main antigenic stimulation at self-cure is derived from substances released by larvae during the third ecdysis.

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