222 The characterisation of steroid receptors in cervical cancer and normal cervix

1983 ◽  
Vol 19 ◽  
pp. 74
Author(s):  
T. Ratajczak ◽  
C. Mamotte ◽  
E. Twaddle ◽  
R. Hähnel
Keyword(s):  
Cervical Cancer ◽  
Steroid Receptors ◽  
Normal Cervix

scholarly journals Prevalence of Cervical Intra Epithelial Neoplasia (CIN) among the sexually active married Women at Rajshahi Division

2019 ◽  
Vol 33 (1) ◽  
pp. 17-20
Author(s):  
Jesmin Shahela ◽  
Najnin Monira ◽  
Ashrafunnesa ◽  
Afroz Romana ◽  
Sarkar Mousumi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Early Stage ◽  
Population Based ◽  
Cross Sectional Study ◽  
Married Women ◽  
Sexually Active ◽  
Cross Sectional ◽  
Socioeconomic Condition ◽  
Normal Cervix ◽  
Good Number

Background: Cervical cancer is 4th most common cancer in women in the world and the most common form of cancer in women in developing countries. Population based cervical cancer screening and treatment of intraepitheial neoplasia in early stage can reduce morbidity and mortality associated with cervical cancer. To determine the prevalence of cervical intra epithelial neoplasia (CIN) among sexually active and married women in Rajshahi Division of Bangladesh. Place and Duration: This population based cross sectional study was carried out in 4 selected Upazilla from 4 different districts of Rajshahi Division from 1st December 2014 to 31st May 2015. Study on 1050 sexually active women between 25 to 55 years of age who fulfilled the inclusion criteria enrolled in the study after taking the consent. Result: Among 1050 enrolled women 90 (8.5%) were VIA positive and 91 were colposcopically abnormal and among them 33 were CIN positive & 58 had chronic cervictis with or without squamorus metaplasia. Mean age of CIN positive women was 36.75 years, 92.2% were housewife & 7.8% were service holder and in 64.6% women education level was up to XII class. In fifty five percent of their husband was day labouer from poor socioeconomic condition. Conclusion: VIA and Colopscopy can differentiate a normal cervix from a precancerous cervix with reasonable accuracy. Till now a good number of studies had been carried out in different countries of world and it is now well established that sensitivity of the test is very good. From the present study we can conclude that it will give a very good information about the prevalence of CIN in our country and by proper management of the CIN cases the incidence of carcinoma cervix can be reduced. Bangladesh J Obstet Gynaecol, 2018; Vol. 33(1) : 17-20

Steroid Receptors and Histological Types in Cervical Cancer

2010 ◽  
Vol 12 (4) ◽  
pp. 541-548
Author(s):  
Jiro Fujimoto ◽  
Teruhiko Tamaya ◽  
Yoshinobu Watanabe ◽  
Kenji Arahori ◽  
Sohei Sato ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Steroid Receptors ◽  
Histological Types

scholarly journals High Levels of Within-Host Variations of Human Papillomavirus 16 E1/E2 Genes in Invasive Cervical Cancer

2020 ◽  
Vol 11 ◽  
Author(s):  
Yusuke Hirose ◽  
Mayuko Yamaguchi-Naka ◽  
Mamiko Onuki ◽  
Yuri Tenjimbayashi ◽  
Nobutaka Tasaka ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Viral Replication ◽  
Viral Genome ◽  
Invasive Cervical Cancer ◽  
Infected Woman ◽  
Human Papillomavirus 16 ◽  
Normal Cervix ◽  
High Level

Human papillomavirus type 16 (HPV16) is the most common HPV genotype found in invasive cervical cancer (ICC). Recent comprehensive genomics studies of HPV16 have revealed that a large number of minor nucleotide variations in the viral genome are present in each infected woman; however, it remains unclear whether such within-host variations of HPV16 are linked to cervical carcinogenesis. Here, by employing next-generation sequencing approaches, we explored the mutational profiles of the HPV16 genome within individual clinical specimens from ICC (n = 31) and normal cervix (n = 21) in greater detail. A total of 367 minor nucleotide variations (167 from ICC and 200 from the normal cervix) were detected throughout the viral genome in both groups, while nucleotide variations at high frequencies (>10% abundance in relative read counts in a single sample) were more prevalent in ICC (10 in ICC versus 1 in normal). Among the high-level variations found in ICC, six were located in the E1/E2 genes, and all of them were non-synonymous substitutions (Q142K, M207I, and L262V for E1; D153Y, R302T, and T357A for E2). In vitro functional analyses of these E1/E2 variants revealed that E1/M207I, E2/D153Y, and E2/R302T had reduced abilities to support viral replication, and that E2/D153Y and E2/R302T failed to suppress the viral early promoter. These results imply that some within-host variations of E1/E2 present at high levels in ICC may be positively selected for and contribute to cervical cancer development through dysfunction or de-stabilization of viral replication/transcription proteins.

scholarly journals Distinct Changes in the Expression TAZ are Associated with Normal Cervix and Human Cervical Cancer

2018 ◽  
Vol 9 (22) ◽  
pp. 4263-4270 ◽  
Author(s):  
Yaofang Liu ◽  
Meiping Ren ◽  
Xiaoyong Tan ◽  
Lina Hu
Keyword(s):  
Cervical Cancer ◽  
Normal Cervix ◽  
Human Cervical Cancer

scholarly journals Differential proteins among normal cervix cells and cervical cancer cells with HPV-16 infection, through mass spectrometry-based Proteomics (2D-DIGE) in women from Southern México

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Idanya Serafín-Higuera ◽  
Olga Lilia Garibay-Cerdenares ◽  
Berenice Illades-Aguiar ◽  
Eugenia Flores-Alfaro ◽  
Marco Antonio Jiménez-López ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cervical Cancer ◽  
Cancer Cells ◽  
Southern Mexico ◽  
Hpv 16 ◽  
2D Dige ◽  
Normal Cervix ◽  
Differential Proteins ◽  
Cervical Cancer Cells

scholarly journals Cytochrome 4Z1 Expression Is Correlated with Poor Prognosis in Patients with Cervical Cancer

2021 ◽  
Vol 28 (5) ◽  
pp. 3573-3584
Author(s):  
Yousef M. Al-saraireh ◽  
Fatemah O. F. O. Alshammari ◽  
Ahmed M. M. Youssef ◽  
Yahya M. Al-sarayra ◽  
Renata A. Al-saraireh ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Lymph Node ◽  
Cancer Patients ◽  
Poor Prognosis ◽  
Patient Survival ◽  
Tumour Stage ◽  
Tumour Invasion ◽  
Cervical Cancers ◽  
Node Metastasis ◽  
Normal Cervix

Background: cervical cancer is one of the most common malignancies in women worldwide and its management remains challenging and complex. As Cytochrome4Z1 (CYP4Z1) is overexpressed in many tumours, its expression in cervical cancer is unknown. Therefore, the present study aimed to evaluate CYP4Z1 expression in cervical cancers. Methods: CYP4Z1 expression was immunohistochemically assessed in 100 cases of cervical cancers along with ten normal cervix tissues, and the enzyme’s relationship to several clinicopathological features and survival was explored. Results: CYP4Z1 was strongly expressed in 55% of cervical cancer patients. Normal cervix samples were negative for CYP4Z1 expression. Importantly, this expression was significantly found in patients with the late stage of the disease, lymph node metastasis, and high tumour invasion (p < 0.05). Interestingly, CYP4Z1 expression was significantly correlated with shorter survival times of cervical cancer patients. Univariate analysis showed that CYP4Z1 expression, tumour stage, lymph node metastasis, and tumour invasion were significantly correlated with patient survival (p < 0.05). The multivariate analysis revealed that only CYP4Z1 expression and tumour stage were significantly correlated with patient survival (p < 0.05). Conclusions: CYP4Z1 expression is associated with cervical cancer patients’ survival and may serve as an independent predictor of poor prognosis in cervical cancer patients.

scholarly journals ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway

2021 ◽  
Vol 10 ◽  
Author(s):  
Yinshuang Wu ◽  
Xixi Chen ◽  
Weijie Dong ◽  
Zhongyang Xu ◽  
Yuli Jian ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Notch Pathway ◽  
Cell Cycle Protein ◽  
Cyclin E1 ◽  
Normal Cervix ◽  
Cervical Cancer Cells ◽  
Negative Role ◽  
Cancer Tissues

ST3Gal IV is one of the principal sialyltransferases responsible for the biosynthesis of α2, 3-sialic acid to the termini N-glycans or O-glycans of glycoproteins and glycolipids. It has been reported that ST3Gal IV expression is associated with gastric carcinoma, pancreatic adenocarcinoma and breast cancer. While the expression and functions of ST3Gal IV in cervical cancer are still poorly understood. In this study, we found that ST3Gal IV was downregulated in human cervical cancer tissues compared to normal cervix tissues, and ST3Gal IV expression was negatively associated with the pathological grade of cervical cancer. ST3Gal IV upregulation inhibited the growth and proliferation of cervical cancer HeLa and SiHa cells in vitro and in vivo. Furthermore, ST3Gal IV overexpression enhanced the expression of several Notch pathway components such as Jagged1, Notch1, Hes1 and Hey1, while cell cycle protein expression like Cyclin D1, Cyclin E1, CDK2 and CDK4 were decreased. These results indicate that expression of ST3Gal IV is reduced in cervical cancer and plays a negative role in cell proliferation via Notch/p21/CDKs signaling pathway. Thus, sialyltransferase ST3Gal IV might be a target for the diagnosis and therapy of cervical cancer.

Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer: identification of candidate genes in pathogenesis of invasion in cervical cancer

2008 ◽  
Vol 18 (5) ◽  
pp. 1051-1059 ◽  
Author(s):  
J. Y. Song ◽  
J. K. Lee ◽  
N. W. Lee ◽  
H. H. Jung ◽  
S. H. Kim ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Candidate Genes ◽  
Expression Profiles ◽  
Invasive Cervical Cancer ◽  
Pcr Analysis ◽  
Cervix Carcinoma ◽  
Rt Pcr ◽  
Cancer Group ◽  
Normal Cervix

The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.

Protein profiling of cervical cancer by protein-biochips: proteomic scoring to discriminate cervical cancer from normal cervix

2004 ◽  
Vol 211 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Y.F Wong ◽  
T.H Cheung ◽  
K.W.K Lo ◽  
V.W Wang ◽  
C.S Chan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Protein Profiling ◽  
Normal Cervix

Identification of Long Noncoding RNA Expression Profiles in HPV-Negative Cervical Cancer

2020 ◽  
Vol 85 (5) ◽  
pp. 377-387
Author(s):  
Shiyin Ooi ◽  
Yuandong Liao ◽  
Pan Liu ◽  
Ganlin Xu ◽  
Tianyu Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Noncoding Rna ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Cell Counting ◽  
Migration And Invasion ◽  
Bioinformatics Analyses ◽  
Qrt Pcr ◽  
Potential Biomarker ◽  
Normal Cervix

<b><i>Aim:</i></b> HPV-negative cervical cancer (CC) usually appears more aggressive and causes poorer survival outcomes compared to HPV-positive cases. However, the research in regard to HPV-negative CC is rare, and the related molecular mechanism underlying remains unclear. We intended to explore the expression profiles of long noncoding RNAs (lncRNAs) and identify the tumor-associated lncRNAs which might be used as the potential biomarker for HPV-negative CC. <b><i>Methods:</i></b> Bioinformatics analyses were utilized to construct the expression profiles of lncRNAs, Gene Ontology, and KEGG analyses and draw the lncRNA-mRNA co-expression network in HPV-negative CC. The expression levels of the top 5 marked-up tumor-associated lncRNAs were detected by qRT-PCR. The effect of LINC00115 on CC growth and metastasis was studied by Cell Counting Kit-8 and transwell assays. <b><i>Results:</i></b> In comparison to normal cervix (NC), 2,052 lncRNAs were differentially expressed in HPV-negative CC. It demonstrated that LINC00115 was significantly upregulated in HPV-negative CC cells compared to NC, and it could promote proliferation, migration, and invasion of HPV-negative CC cells. <b><i>Conclusion:</i></b> LINC00115 might be a potential biomarker for HPV-negative CC.

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