Mitotic metaphase cells from different cell lines cause different levels of expression of the α-form of interphase chromosome breaks irradiated CHO cels

1994 ◽  
Vol 310 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Ryuichi Okayasu ◽  
George Iliakis
Keyword(s):  
Cell Lines ◽  
Mitotic Metaphase ◽  
Interphase Chromosome ◽  
Chromosome Breaks ◽  
Different Levels

scholarly journals Epstein-Barr Virus Recombinants from BC-1 and BC-2 Can Immortalize Human Primary B Lymphocytes with Different Levels of Efficiency and in the Absence of Coinfection by Kaposi's Sarcoma-Associated Herpesvirus

2000 ◽  
Vol 74 (2) ◽  
pp. 735-743 ◽  
Author(s):  
Andrew J. Aguirre ◽  
Erle S. Robertson
Keyword(s):  
B Lymphocytes ◽  
Cell Lines ◽  
Kaposi's Sarcoma ◽  
Epstein Barr Virus ◽  
Kaposi’S Sarcoma ◽  
Barr Virus ◽  
Kaposi's Sarcoma Associated Herpesvirus ◽  
Epstein Barr ◽  
Kaposi’S Sarcoma Associated Herpesvirus ◽  
Different Levels

ABSTRACT Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are human gammaherpesviruses associated with numerous malignancies. Primary effusion lymphoma or body cavity-based lymphoma is a distinct clinicopathological entity that, in the majority of cases, manifests coinfection with KSHV and EBV. In previous analyses, we have characterized the EBV in the BC-1 and BC-2 cell lines as potential intertypic recombinants of the EBV types 1 and 2. In order to examine the infectious and transforming capacities of KSHV and the intertypic EBV recombinants from the BC-1 and BC-2 cell lines, viral replication was induced in these cell lines and fresh human primary B lymphocytes were infected with progeny virus. The transformed clones were analyzed by PCR and Western blotting. All analyzed clones were infected with the intertypic progeny EBV but had no detectable signal for progeny KSHV. Additionally, primary B lymphocytes incubated with viral supernatant containing KSHV alone showed an unsustained initial proliferation, but prolonged growth or immortalization of these cells in vitro was not observed. We also show that the EBV recombinants from BC-1 were less efficient than the EBV recombinants from BC-2 in the ability to maintain the transformed phenotype of the infected human B lymphocytes. From these findings, we conclude that the BC-1 and BC-2 intertypic EBV recombinants can immortalize human primary B lymphocytes, albeit at different levels of efficiency. However, the KSHV induced from BC-1 and BC-2 alone cannot transform primary B cells, nor can it coinfect EBV-positive B lymphocytes under our experimental conditions with B lymphocytes from EBV-seropositive individuals. These results are distinct from those in one previous report and suggest a possible requirement for other factors to establish coinfection with both viral agents.

scholarly journals Deciphering the Functional Role of RIPK4 in Melanoma

2021 ◽  
Vol 22 (21) ◽  
pp. 11504
Author(s):  
Ewelina Madej ◽  
Damian Ryszawy ◽  
Anna A. Brożyna ◽  
Malgorzata Czyz ◽  
Jaroslaw Czyz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Time Lapse ◽  
Clinical Samples ◽  
Invasive Potential ◽  
Interacting Protein ◽  
Metastatic Melanoma Cell ◽  
Node Metastasis ◽  
Different Levels

The receptor-interacting protein kinase 4 (RIPK4) plays an important role in the development and maintenance of various tissues including skin, but its role in melanoma has not been reported. Using patient-derived cell lines and clinical samples, we show that RIPK4 is expressed in melanomas at different levels. This heterogenous expression, together with very low level of RIPK4 in melanocytes, indicates that the role of this kinase in melanoma is context-dependent. While the analysis of microarray data has revealed no straightforward correlation between the stage of melanoma progression and RIPK4 expression in vivo, relatively high levels of RIPK4 are in metastatic melanoma cell lines. RIPK4 down-regulation by siRNA resulted in the attenuation of invasive potential as assessed by time-lapse video microscopy, wound-healing and transmigration assays. These effects were accompanied by reduced level of pro-invasive proteins such as MMP9, MMP2, and N-cadherin. Incubation of melanoma cells with phorbol ester (PMA) increased PKC-1β level and hyperphosphorylation of RIPK4 resulting in degradation of RIPK4. Interestingly, incubation of cells with PMA for short and long durations revealed that cell migration is controlled by the NF-κB signaling in a RIPK4-dependent (RIPK4high) or independent (RIPK4low) manner depending on cell origin (distant or lymph node metastasis) or phenotype (mesenchymal or epithelial).

scholarly journals A New Metabolite from the Endophytic Fungus Penicillium citrinum

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Xinlan Li ◽  
Liang Zhang ◽  
Yanhui Liu ◽  
Zhiyong Guo ◽  
Zhangshuang Deng ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Endophytic Fungus ◽  
Cancer Cell Lines ◽  
Penicillium Citrinum ◽  
Hep G2 ◽  
Different Levels

A new polyketide, penicillocitrin A (1), together with four known compounds, was isolated from the endophytic fungus Penicillium citrinum (CTGU-TS-24) of Tapiscia sinensis Oliv., and their structures were elucidated by NMR spectroscopic and MS spectrums. The five compounds were evaluated cytotoxic activity to four cancer cell lines A549, Hep G2, Hela and Caski at different levels.

scholarly journals Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1323
Author(s):  
Jua Lee ◽  
Heeyoun Hwang ◽  
Sumin Kim ◽  
Jaeyun Hwang ◽  
Jaekyung Yoon ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Types ◽  
Cellular Membrane ◽  
Abundant Species ◽  
Invasion And Metastasis ◽  
Pancreatic Cell ◽  
Analytical Tools ◽  
Different Levels

Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski. We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS. Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively. The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively. Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties. In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1). These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.

scholarly journals PUROMYCIN RESISTANCE IN HAPLOID AND HETEROPLOID FROG CELLS: GENE OR MEMBRANE DETERMINED?

1971 ◽  
Vol 51 (3) ◽  
pp. 742-751 ◽  
Author(s):  
Liselotte Mezger-Freed
Keyword(s):  
Membrane Permeability ◽  
Gene Mutation ◽  
Cell Lines ◽  
Colony Formation ◽  
Cell Populations ◽  
Resistant Phenotype ◽  
Mutagen Treatment ◽  
Different Levels

The frequency of colony formation in monolayers of cultured frog cell lines treated with puromycin was compared in (a) haploid and heteroploid lines and (b) mutagen-treated and nontreated haploid lines. Evidence that resistant colonies result from gene mutation was negative, since the colony frequency is independent of both ploidy and mutagen treatment. A study of five frog cell lines showed that colony formation in puromycin depends on (a) the concentration of puromycin, (b) preselection of the population with puromycin, and, particularly, (c) the capacity of the treated population to survive some exposure to puromycin. One haploid and one heteroploid strain showing stable resistance to puromycin have been isolated; comparison of those variants with sensitive populations has shown that resistance to puromycin is correlated with the cells' capacity to exclude the drug. The evidence for different levels of membrane permeability, combined with evidence for many degrees of resistance among and within cell populations, suggests a model of self-determining membrane units. The evolution of a resistant phenotype may result from changes in the proportion of specific units in the membrane population.

Sign in / Sign up

Export Citation Format

Share Document