Disruption of myelin sheaths in mouse brain in vitro and in vivo by staphylococcal α-toxin

A mouse brain spectrin-like protein, which was an immunoreactive analogue of erythrocyte spectrin, has been isolated from demyelinated membranes. This spectrin analogue was a 10.5 S, 972,000 molecular weight (Mr) (alpha beta)2 tetramer containing subunits of 240,000 (alpha) and 235,000 (beta) Mr. We demonstrated that in vivo only the 235,000 Mr beta subunit of the mouse brain spectrin-like protein was phosphorylated, which was an analogous situation to mouse erythrocyte spectrin in which only the 220,000 Mr beta subunit was phosphorylated. Incubation of isolated membrane fractions with [gamma-32P]ATP +/- adenosine 3',5'-cyclic monophosphate (cAMP) indicated that mouse brain spectrin-like protein, mouse erythrocyte spectrin, and human erythrocyte spectrin's beta subunits were all phosphorylated in vitro by membrane-associated cAMP-independent protein kinases.

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Cannabinoid effects on adenylate cyclase and phosphodiesterase activities of mouse brain

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y77-125 ◽

1977 ◽

Vol 55 (4) ◽

pp. 934-942 ◽

Cited By ~ 10

Author(s):

Thomas W. Dolby ◽

Lewis J. Kleinsmith

Keyword(s):

Cyclic Amp ◽

Adenylate Cyclase ◽

Mouse Brain ◽

Biogenic Amine ◽

Specific Activity ◽

Intraperitoneal Administration ◽

The Brain

The experiments presented in this paper examine the mechanisms underlying the ability of cannabinoids to alter the in vivo levels of cyclic adenosine 3′,5′-monophosphate (cyclic AMP) in mouse brain. It was found that changes in cyclic AMP levels are a composite result of direct actions of cannabinoids on adenylate cyclase (EC 4.6.1.1) activity and indirect actions involving the potentiation or inhibition of biogenic amine induced activity of adenylate cyclase. Furthermore, the long-term intraperitoneal administration of 1-(−)-Δ-tetrahydrocannabinol to mice produced a form of phosphodiesterase (EC 3.1.4.17) in the brain whose activity is not stimulated by Ca2+, although its basal specific activity is similar to that of control animals. In vitro, the presence of the cannabinoids caused no significant changes in activity of brain PDE at the concentrations tested. Some correlations are presented which imply that many of the observed behavioral and physiological actions of the cannabinoids in mammalian organisms may be mediated via cyclic AMP mechanisms.

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In vitro and In vivo Assessment of Suitable Reference Region and Kinetic Modelling for the mGluR1 Radioligand [11C]ITDM in Mice

Molecular Imaging and Biology ◽

10.1007/s11307-019-01435-1 ◽

2019 ◽

Vol 22 (4) ◽

pp. 854-863 ◽

Cited By ~ 4

Author(s):

Daniele Bertoglio ◽

Jeroen Verhaeghe ◽

Špela Korat ◽

Alan Miranda ◽

Leonie wyffels ◽

...

Keyword(s):

Mouse Brain ◽

Pet Imaging ◽

Metabotropic Glutamate Receptor ◽

Kinetic Modelling ◽

Input Function ◽

Dependent Manner ◽

Reference Region ◽

Suitable Reference

Abstract Purpose This study aimed at investigating binding specificity, suitability of reference region-based kinetic modelling, and pharmacokinetics of the metabotropic glutamate receptor 1 (mGluR1) radioligand [11C]ITDM in mice. Procedures We performed in vivo blocking as well as displacement of [11C]ITDM during positron emission tomography (PET) imaging using the specific mGluR1 antagonist YM-202074. Additionally, we assessed in vitro blocking of [3H]ITDM at two different doses of YM-202074. As an alternative to reference region models, we validated the use of a noninvasive image-derived input function (IDIF) compared to an arterial input function measured with an invasive arteriovenous (AV) shunt using a population-based curve for radiometabolite correction and characterized the pharmacokinetic modelling of [11C]ITDM in the mouse brain. Finally, we also assessed semi-quantitative approaches. Results In vivo blocking with YM-202074 resulted in a decreased [11C]ITDM binding, ranging from − 35.8 ± 8.0 % in pons to − 65.8 ± 3.0 % in thalamus. Displacement was also markedly observed in all tested regions. In addition, in vitro [3H]ITDM binding could be blocked in a dose-dependent manner. The volume of distribution (VT) based on the noninvasive IDIF (VT (IDIF)) showed excellent agreement with the VT values based on the metabolite-corrected plasma input function regardless of the metabolite correction (r2 > 0.943, p < 0.0001). Two-tissue compartmental model (2TCM) was found to be the preferred model and showed optimal agreement with Logan plot (r2 > 0.960, p < 0.0001). A minimum scan duration of 80 min was required for proper parameter estimation. SUV was not reliable (r2 = 0.379, p = 0.0011), unlike the SUV ratio to the SUV of the input function, which showed to be a valid approach. Conclusions No suitable reference region could be identified for [11C]ITDM as strongly supported by in vivo and in vitro evidence of specific binding in all brain regions. However, by applying appropriate kinetic models, [11C]ITDM PET imaging represents a promising tool to visualize mGluR1 in the mouse brain.

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Vulnerability of rat and mouse brain cells to murine hepatitis virus (JHM-strain): Studies in vivo and in vitro

Glia ◽

10.1002/glia.440020204 ◽

1989 ◽

Vol 2 (2) ◽

pp. 85-93 ◽

Cited By ~ 5

Author(s):

M.F. van Berlo ◽

R. Warringa ◽

G. Wolswijk ◽

M. Lopes-Cardozo

Keyword(s):

Mouse Brain ◽

Hepatitis Virus ◽

Brain Cells ◽

Murine Hepatitis Virus

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Ionising Radiation Immediately Impairs Synaptic Plasticity-Associated Cytoskeletal Signalling Pathways in HT22 Cells and in Mouse Brain: An In Vitro/In Vivo Comparison Study

PLoS ONE ◽

10.1371/journal.pone.0110464 ◽

2014 ◽

Vol 9 (10) ◽

pp. e110464 ◽

Cited By ~ 28

Author(s):

Stefan J. Kempf ◽

Sonja Buratovic ◽

Christine von Toerne ◽

Simone Moertl ◽

Bo Stenerlöw ◽

...

Keyword(s):

Synaptic Plasticity ◽

Mouse Brain ◽

Ionising Radiation ◽

Signalling Pathways ◽

Comparison Study ◽

Ht22 Cells

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Purple Sweet Potato Color Ameliorates Cognition Deficits and Attenuates Oxidative Damage and Inflammation in Aging Mouse Brain Induced by D-Galactose

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/564737 ◽

2009 ◽

Vol 2009 ◽

pp. 1-9 ◽

Cited By ~ 57

Author(s):

Qun Shan ◽

Jun Lu ◽

Yuanlin Zheng ◽

Jing Li ◽

Zhong Zhou ◽

...

Keyword(s):

Sweet Potato ◽

Mouse Brain ◽

Nuclear Translocation ◽

Neuroprotective Effect ◽

Neuroprotective Effects ◽

Copper Zinc Superoxide Dismutase ◽

Aging Mouse ◽

Purple Sweet Potato

Purple sweet potato color (PSPC), a naturally occurring anthocyanin, has a powerful antioxidant activity in vitro and in vivo. This study explores whether PSPC has the neuroprotective effect on the aging mouse brain induced by D-galactose (D-gal). The mice administrated with PSPC (100 mg/kg.day, 4 weeks, from 9th week) via oral gavage showed significantly improved behavior performance in the open field and passive avoidance test compared with D-gal-treated mice (500 mg/kg.day, 8 weeks). We further investigate the mechanism involved in neuroprotective effects of PSPC on mouse brain. Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), inhibited nuclear translocation of nuclear factor-kappaB (NF-κB), increased the activity of copper/zinc superoxide dismutase (Cu/Zn-SOD) and catalase (CAT), and reduced the content of malondialdehyde (MDA), respectively. Our data suggested that PSPC attenuated D-gal-induced cognitive impairment partly via enhancing the antioxidant and anti-inflammatory capacity.

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In vivo and in vitro effects of aluminum on the activity of mouse brain acetylcholinesterase

Brain Research Bulletin ◽

10.1016/s0361-9230(02)00836-5 ◽

2002 ◽

Vol 59 (1) ◽

pp. 41-45 ◽

Cited By ~ 90

Author(s):

P Zatta ◽

M Ibn-Lkhayat-Idrissi ◽

P Zambenedetti ◽

M Kilyen ◽

T Kiss

Keyword(s):

Mouse Brain ◽

In Vitro Effects ◽

Brain Acetylcholinesterase

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Corrigendum to “Mouse brain plasmalogens are targets for hypochlorous acid-mediated modification in vitro and in vivo” [Free Radic. Biol. Med. 49 (2010) 1655-1665]

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2011.01.008 ◽

2011 ◽

Vol 50 (7) ◽

pp. 903

Author(s):

Andreas Üllen ◽

Günter Fauler ◽

Harald Köfeler ◽

Sabine Waltl ◽

Christoph Nusshold ◽

...

Keyword(s):

Mouse Brain ◽

Hypochlorous Acid ◽

Free Radic Biol

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Quantitative secretome analysis establishes the cell type-resolved mouse brain secretome

10.1101/2020.05.22.110023 ◽

2020 ◽

Author(s):

Johanna Tüshaus ◽

Stephan A. Müller ◽

Evans Sioma Kataka ◽

Jan Zaucha ◽

Laura Sebastian Monasor ◽

...

Keyword(s):

Mouse Brain ◽

High Performance ◽

Brain Slices ◽

Large Cell ◽

Cell Type ◽

Cellular Origin ◽

Secretome Analysis ◽

Csf Proteins

AbstractTo understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the high-performance secretome-protein-enrichment-with-click-sugars method (hiSPECS). To demonstrate its broad utility, hiSPECS was used to identify the secretory response of brain slices upon LPS-induced neuroinflammation and to establish the cell type-resolved mouse brain secretome resource using primary astrocytes, microglia, neurons and oligodendrocytes. This resource allowed mapping the cellular origin of CSF proteins and revealed that an unexpectedly high number of secreted proteins in vitro and in vivo are proteolytically-cleaved membrane protein ectodomains. Two examples are neuronally secreted ADAM22 and CD200, which we identified as substrates of the Alzheimer-linked protease BACE1. hiSPECS and the brain secretome resource can be widely exploited to systematically study protein secretion, brain function and to identify cell type-specific biomarkers for CNS diseases.

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Multiple Irradiation Affects Cellular and Extracellular Components of the Mouse Brain Tissue and Adhesion and Proliferation of Glioblastoma Cells in Experimental System In Vivo

International Journal of Molecular Sciences ◽

10.3390/ijms222413350 ◽

2021 ◽

Vol 22 (24) ◽

pp. 13350

Author(s):

Maxim O. Politko ◽

Alexandra Y. Tsidulko ◽

Oxana A. Pashkovskaya ◽

Konstantin E. Kuper ◽

Anastasia V. Suhovskih ◽

...

Keyword(s):

Brain Tissue ◽

Mouse Brain ◽

Glial Cells ◽

Ex Vivo ◽

Experimental System ◽

Normal Brain ◽

Biosynthetic Activity ◽

Normal Brain Tissue

Intensive adjuvant radiotherapy (RT) is a standard treatment for glioblastoma multiforme (GBM) patients; however, its effect on the normal brain tissue remains unclear. Here, we investigated the short-term effects of multiple irradiation on the cellular and extracellular glycosylated components of normal brain tissue and their functional significance. Triple irradiation (7 Gy*3 days) of C57Bl/6 mouse brain inhibited the viability, proliferation and biosynthetic activity of normal glial cells, resulting in a fast brain-zone-dependent deregulation of the expression of proteoglycans (PGs) (decorin, biglycan, versican, brevican and CD44). Complex time-point-specific (24–72 h) changes in decorin and brevican protein and chondroitin sulfate (CS) and heparan sulfate (HS) content suggested deterioration of the PGs glycosylation in irradiated brain tissue, while the transcriptional activity of HS-biosynthetic system remained unchanged. The primary glial cultures and organotypic slices from triple-irradiated brain tissue were more susceptible to GBM U87 cells’ adhesion and proliferation in co-culture systems in vitro and ex vivo. In summary, multiple irradiation affects glycosylated components of normal brain extracellular matrix (ECM) through inhibition of the functional activity of normal glial cells. The changed content and pattern of PGs and GAGs in irradiated brain tissues are accompanied by the increased adhesion and proliferation of GBM cells, suggesting a novel molecular mechanism of negative side-effects of anti-GBM radiotherapy.

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