Phosphorus release-storage reaction and organic substrate behavior in biological phosphorus removal

1988 ◽  
Vol 22 (1) ◽  
pp. 49-58 ◽  
Author(s):  
I. Somiya ◽  
H. Tsuno ◽  
M. Matsumoto
1992 ◽  
Vol 25 (12) ◽  
pp. 165-174 ◽  
Author(s):  
R. Franci Gonçalves ◽  
F. Rogalla

Possible procedures to achieve biological phosphorus removal in a fixed film reactor are discussed and the feasibility of phosphorus removal process in a fixed film reactor under continous flow is demonstrated. The behaviour of an upflow aerated filter operating under continuous feed and alternate aerobic/anaerobic conditions is analyzed. The influence of the duration of anaerobic and aerobic contact periods and of organic substrate loadings on the phosphorus removal process is verified. During the anaerobic state, the longer the duration, or the higher the substrate load, the better the phosphorus release and consequently the higher the uptake in the aerobic phase. The excess of accumulated phosphorus in the aerobic phase over released phosphorus in the anaerobic phase approaches 33 %. For each mg of phosphorus released, 5 mg filtered COD are consumed. Continuous phosphorus removal on two biofilters in series was performed by alternating aeration conditions, always introducing the influent to the anaerobic reactor.The tests carried out on laboratory scale showed that this system carrys out complete nitrification and removal of 80% of the phosphorus with a maximum hydraulic retention time of 5 hours. The hydraulic retention time and the residence time of the biomass in the reactor are independent and, therefore, the time the bacteria are exposed to alternate A/O conditions can be optimized. The very low concentrations of suspended solids in the effluent of the biofilter enable residual levels below 1 mg PO4-P/l to be obtained. Further investigations are carried out on full scale and to introduce denitrification in the same reactor.


1985 ◽  
Vol 17 (11-12) ◽  
pp. 297-298 ◽  
Author(s):  
Takao Murakami ◽  
Atsushi Miyairi ◽  
Kazuhiro Tanaka

In Japan various biological phosphorus removal processes have recently been researched by laboratory or pilot plant scale studies and most of them have shown good results. Based on these results, the Japan Sewage Works Agency has conducted a full scale study of the biological phosphorus removal process from June 1982 until February 1983, which was the first full scale operation of this process in Japan. The main purpose of the study was to evaluate phosphorus removal efficiency and also nitrogen removal efficiency of the process and in addition, to ascertain the important operating factors of the process. For the study a treatment train of a large scale sewage treatment plant was remodelled. The aeration tank of 3.825 m3 volume was divided into four equal cells. The whole train including return sludge line was operated entirely independently of the other trains. During the experiment the train was operated under two different modes, Mode 1 and Mode 2. In Mode 1, the train was operated as an A/O process, the first cell of the aeration tank being anaerobic and the other cells oxic. In Mode 2, the train was operated as a Modified Phoredox process. In this case, the first cell was anaerobic, but the second cell was anoxic and nitrified liquor was returned to it from the end of the oxic cells. Mode 1 and Mode 2 were further divided into many ‘runs' and the flow rate varied between 12,550 m3 d−1 and 25,270 m3 d−1 , corresponding to retention times of 7.3 hours and 3.6 hours, respectively. Throughout the experimental period the mean value of influent (primary effluent) total-P concentration was 3.38 mg 1−1 , and that of the final effluent was 0.47 mg 1−1 . A cumulated frequency curve of the data showed that about 93% of measured effluent total-P was below 1.0 mg l−1 . Therefore, it can be concluded that with these influent total-P levels, biological phosphorus removal processes can sufficiently satisfy the effluent standard of 1 mg 1−1 total-P. Even when the process was operated as a Modified Phoredox Process, no obstruction to phosphorus removal because of nitrification was observed and phosphorus removal remained good. However, since the sewage treatment plant treated influent from a combined sewerage system, phosphorus removal was sometimes affected by heavy rainfalls. In such cases phosphorus release in the anaerobic cell was insufficient because of increased influent NOx concentration and accordingly increased denitrification level in the anaerobic cell. Therefore, as a result, enhanced phosphorus uptake in the following cells could not be observed. Higher process stability can be expected if an effective countermeasure to high influent NOx concentration can be made. Influence of flow rate fluctuation on the process was also studied. The treatment train was operated for a week under a daily flow rate fluctuation pattern which ranged between 460 m3 hr−1 and 820 m3 hr−1 . Nevertheless, the effluent total-P concentration showed no increase and stayed constantly lower than 0.5 mg 1−1. The oxidation reduction potential (ORP) was an effective control index to evaluate the degree of phosphorus release in the anaerobic cell. Water temperature did not affect phosphorus release and uptake rates.


2000 ◽  
Vol 41 (4-5) ◽  
pp. 503-508 ◽  
Author(s):  
R.F. Gonçalves ◽  
F. Rogalla

This work describes laboratory scale research about Enhanced Biological Phosphorus Removal (EBPR) in a submerged biofilter under Anaerobic/Oxic (A/O) alternation and continuous feed. Its main purpose is to detail the behaviour of the reactor throughout the anaerobic and the aerobic phases of the A/O cycle, to study the importance of the anaerobic phase in the selection of the EBPR bacteria in the biofilm and to evaluate the consumption and the importance of the organic substrate during the anaerobic phase. The mass balance over the Phosphorus (P) element indicates that long anaerobic phases (6 h) are more efficient than short ones (3 h) as a selector of EBPR bacteria in biofilms. In both comparisons, thespecific mass of P released in a 6 h period represents almost 50% more than the amount of P release in the shorter period (3 h). However, the presence of rapidly biodegradable COD in the influent of the anaerobic phase is a more effective selector, more important than the duration of the anaerobic phase: by doubling the amount of acetic acid in the influent, a similar 50% increase of P-release can be achieved at short anaerobic periods of 3 h. The effect of the strategy adopted in this study, focusing on selecting EBPR bacteria in biofilm, is shown by the P levels of 4% (total P/SST) in the sludge removed from the BF by backwashing in all periods.


2002 ◽  
Vol 46 (4-5) ◽  
pp. 193-200 ◽  
Author(s):  
D. Brandt ◽  
C. Sieker ◽  
W. Hegemann

The sorption-denitrification-P-removal (S-DN-P) process combines biological excess P-removal (BEPR) and denitrification using immobilized biomass. The accumulation of denitrifying polyP organisms is achieved by sequencing anaerobic/anoxic conditions. The immobilized biomass is in alternating contact with primary treated wastewater (anaerobic sorption-phase) and nitrified wastewater (denitrification phase). In the sorption phase, P-release takes place and readily biodegradable organic substrate, e.g. volatile fatty acid, is taken up and stored by polyP accumulating organisms (PAO). In addition to this, other organic matter is physically/chemically adsorbed in the biofilm structures. In the denitrification phase, the biomass denitrifies the stored and adsorbed organic substrate and, at the same time, P-uptake and polyP formation occurs. This paper presents results of investigations at laboratory and half-technical scale. At laboratory scale different types of carriers were tested regarding their suitability for the S-DN-P-process. In half-technical scale a biofilter and a moving bed reactor (MBR) were tested. In the biofilter a stable removal of nitrate and phosphate was achieved. However, it was not possible to achieve similar results in the MBR process. Especially the release and uptake of phosphate showed no clear tendency although the uptake of acetate was good. Reasons for this could be the accumulation of glycogen accumulating organisms which impair the metabolism of PAO.


1998 ◽  
Vol 38 (1) ◽  
pp. 97-105 ◽  
Author(s):  
J. Meinhold ◽  
H. Pedersen ◽  
E. Arnold ◽  
S. Isaacs ◽  
M. Henze

The continuous introduction of a biological phosphorus removal (BPR) promoting organic substrate to the denitrifying reactor of a BPR process is examined through a series of batch experiments using acetate as model organic substrate. Several observations are made regarding the influence of substrate availability on PHA storage/utilization and phosphate uptake/release. Under anoxic conditions PHB is utilized and phosphate is taken up, indicating that at least a fraction of the PAO can denitrify. The rates of anoxic P-uptake, PHB utilization and denitrification are found to increase with increasing initial PHB level. At low acetate addition rates the P-uptake and PHB utilization rates are reduced compared to when no acetate is available. At higher acetate addition rates a net P-release occurs and PHB is accumulated. For certain intermediate acetate addition rates the PHB level can increase while a net P-release occurs. Whether the introduction of BPR promoting organic substrates to the denitrifying reactor is detrimental to overall P-removal appears to be dependent on the interaction between aerobic P-uptake, which is a function of PHB level, and the aerobic residence time.


1993 ◽  
Vol 28 (10) ◽  
pp. 377-385 ◽  
Author(s):  
K. Moriyama ◽  
M. Takahashi ◽  
Y. Harada

Some experiences concerning a retrofit design and operation for nutrients removal in small extended aeration plants are presented. In this study a new biological phosphorus removal process as well as a biological nitrogen removal process based on a sequential oxic-anoxic-oxic process is investigated. The denitrification in the first oxic zone has a high removing effect for nitrogen, and the oxidation reduction potential (ORP) value and dissolved oxygen (DO) concentration are useful indexes to maintain the optimum conditions of the first oxic zone for simultaneous denitrification. It is verified that the simultaneous denitrification can alleviate the alkalinity deficit problem against full nitrification by a stoichiometric analysis of alkalinity throughout the entire process. Additionally, a biological phosphorus removal process which uses a sludge blanket zone of secondary settling tank for phosphorus release is proposed.


1997 ◽  
Vol 36 (12) ◽  
pp. 55-60 ◽  
Author(s):  
S. W. Oa ◽  
E. Choi

Phosphorus removal characteristics are rather complicated in a highly nitrogenous waste like nightsoil under treatment with SBR (sequencing batch reactor). It was found that the increased pH due to denitrification in anaerobic period stimulated chemical precipitation of phosphorus as struvite and hydroxyapatite, and the depressed pH due to nitrification in the aerobic period dissolved the previously formed precipitates. Phosphate accumulating organisms (PAO) worked as in the ordinary BNR (biological nutrient removal) systems regardless of the chemical reactions, but the chemical reactions masked the biological phosphorus release and uptake reactions. About 36% of phosphorus applied was removed biologically in polyphosphate granules. P-fractionation of sludges confirmed this phenomenon. Biological phosphorus removal could be increased with the increased anaerobic period. The morphological types of phosphorus precipitates were examined by SEM in combination with x-ray diffraction.


1991 ◽  
Vol 23 (4-6) ◽  
pp. 585-594 ◽  
Author(s):  
Z. H. Abu-ghararah ◽  
C. W. Randall

The effect of influent organic compounds on the performance of a biological nutrient removal system was investigated using a pilot plant system operated as a UCT (University of Cape Town) process. The system was fed domestic sewage and operated at a sludge age of 13 days. The effects of separate addition of formic, acetic, propionic, butyric, isobutyric, valeric, and isovaleric acid on phosphorus release under anaerobic conditions, and phosphorus uptake under aerobic conditions, were studied. The effects of the organic acid additions on the removal of nitrogen and COD, and changes in SOUR and MLSS, were also studied. All added substrates, except formic acid, caused significant increases in phosphorus release in the anaerobic stage, and subsequent phosphorus uptake in the aerobic stage with an increase in phosphorus removal efficiency. It was also found that the branched organic acids, isobutyric and isovaleric, caused more phosphorus release in the anaerobic stage and better phosphorus removal efficiencies in the system, compared with the nonbranching forms of the same organic acids. The most recent biochemical model, proposed by Comeau et al. (1986) and Wentzel et al. (1986) was also tested using the data collected in this investigation. Both models, in most cases, overestimated the ratios of phosphorus release to volatile fatty acid utilized. All added substrates caused no change in either COD or TKN removals. For engineering applications, it is suggested by this research, that at least 20 mg COD equivalent of acetic acid is needed for the removal of 1 mg phosphorus.


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