mab-3, a gene required for sex-specific yolk protein expression and a male-specific lineage in C. elegans

Cell ◽  
1988 ◽  
Vol 54 (7) ◽  
pp. 1019-1031 ◽  
Author(s):  
Michael M. Shen ◽  
Jonathan Hodgkin
Keyword(s):  
Protein Expression ◽  
Yolk Protein ◽  
C Elegans ◽  
Male Specific

Caenorhabditis elegans lin-25: cellular focus, protein expression and requirement for sur-2 during induction of vulval fates

Development ◽  
1998 ◽  
Vol 125 (23) ◽  
pp. 4809-4819 ◽  
Author(s):  
L. Nilsson ◽  
X. Li ◽  
T. Tiensuu ◽  
R. Auty ◽  
I. Greenwald ◽  
...  
Keyword(s):  
Protein Expression ◽  
Map Kinase ◽  
Signal Transduction Pathway ◽  
Precursor Cells ◽  
C Elegans ◽  
Novel Proteins ◽  
Genetic Mosaic ◽  
Mosaic Analysis ◽  
Map Kinase Pathway ◽  
Cell Fusions

Induction of vulval fates in the C. elegans hermaphrodite is mediated by a signal transduction pathway involving Ras and MAP kinase. Previous genetic analysis has suggested that two potential targets of this pathway in the vulva precursor cells are two novel proteins, LIN-25 and SUR-2. In this report, we describe further studies of lin-25. The results of a genetic mosaic analysis together with those of experiments in which lin-25 was expressed under the control of an heterologous promoter suggest that the major focus of lin-25 during vulva induction is the vulva precursor cells themselves. We have generated antisera to LIN-25 and used these to analyse the pattern of protein expression. LIN-25 is present in all six precursor cells prior to and during vulva induction but later becomes restricted to cells of the vulval lineages. Mutations in genes in the Ras/MAP kinase pathway do not affect the pattern of expression but the accumulation of LIN-25 is reduced in the absence of sur-2. Overexpression of LIN-25 does not rescue sur-2 mutant defects suggesting that LIN-25 and SUR-2 may function together. LIN-25 is also expressed in the lateral hypodermis. Overexpression of LIN-25 disrupts lateral hypodermal cell fusion, suggesting that lin-25 may play a role in regulating cell fusions in C. elegans.

scholarly journals Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Douglas K. Reilly ◽  
Emily J. McGlame ◽  
Elke Vandewyer ◽  
Annalise N. Robidoux ◽  
Caroline S. Muirhead ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Behavioral Response ◽  
Receptor Expression ◽  
Specific Response ◽  
Sensory Cues ◽  
Animal Kingdom ◽  
Dioecious Species ◽  
C Elegans ◽  
Biochemical Studies ◽  
Male Specific

AbstractDioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.

scholarly journals Conservation of mRNA and Protein Expression during Development of C. elegans

Cell Reports ◽  
2014 ◽  
Vol 6 (3) ◽  
pp. 565-577 ◽  
Author(s):  
Dominic Grün ◽  
Marieluise Kirchner ◽  
Nadine Thierfelder ◽  
Marlon Stoeckius ◽  
Matthias Selbach ◽  
...  
Keyword(s):  
Protein Expression ◽  
C Elegans ◽  
Mrna And Protein Expression

scholarly journals New genetic regulators question relevance of abundant yolk protein production in C. elegans

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Liesbeth Van Rompay ◽  
Charline Borghgraef ◽  
Isabel Beets ◽  
Jelle Caers ◽  
Liesbet Temmerman
Keyword(s):  
Protein Production ◽  
Yolk Protein ◽  
C Elegans

scholarly journals In vivo measurements reveal a single 5’ intron is sufficient to increase protein expression level in C. elegans

2018 ◽  
Author(s):  
Matthew M. Crane ◽  
Bryan Sands ◽  
Christian Battaglia ◽  
Brock Johnson ◽  
Soo Yun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Expression Level ◽  
Protein Activity ◽  
Coding Sequence ◽  
Expression Levels ◽  
C Elegans ◽  
Hsp 90 ◽  
Gene Expression Levels

AbstractIntrons can increase gene expression levels using a variety of mechanisms collectively referred to as Intron Mediated Enhancement (IME). To date, the magnitude of IME has been quantified in human cell culture and plant models by comparing intronless reporter gene expression levels to those of intron-bearing reporter genes in vitro (mRNA, Western Blots, protein activity), using genome editing technologies that lacked full control of locus and copy number. Here, for the first time, we quantified IME in vivo, in terms of protein expression levels, using fluorescent reporter proteins expressed from a single, defined locus in Caenorhabditis elegans. To quantify the magnitude of IME, we developed a microfluidic chip-based workflow to mount and image individual animals, including software for operation and image processing. We used this workflow to systematically test the effects of position, number and sequence of introns on two different proteins, mCherry and mEGFP, driven by two different promoters, vit-2 and hsp-90. We found the three canonical synthetic introns commonly used in C. elegans transgenes increased mCherry protein concentration by approximately 50%. The naturally-occurring introns found in hsp-90 also increased mCherry expression level by about 50%. Furthermore, and consistent with prior results examining mRNA levels, protein activity or phenotypic rescue, we found that a single, natural or synthetic, 5’ intron was sufficient for the full IME effect while a 3’ intron was not. IME was also affected by protein coding sequence (50% for mCherry and 80% for mEGFP) but not strongly affected by promoter 46% for hsp-90 and 54% for the stronger vit-2. Our results show that IME of protein expression in C. elegans is affected by intron position and contextual coding sequence surrounding the introns, but not greatly by promoter strength. Our combined controlled transgenesis and microfluidic screening approach should facilitate screens for factors affecting IME and other intron-dependent processes.

scholarly journals The mutational decay of male and hermaphrodite competitive fitness in the androdioecious nematode C. elegans, in which males are naturally rare

2017 ◽  
Author(s):  
Shu-Dan Yeh ◽  
Ayush Saxena ◽  
Timothy Crombie ◽  
Dorian Feistel ◽  
Lindsay M. Johnson ◽  
...  
Keyword(s):  
High Frequency ◽  
Low Frequency ◽  
Point Estimate ◽  
Male Mating ◽  
Male Fitness ◽  
Competitive Fitness ◽  
C Elegans ◽  
Self Fertilization ◽  
Male Specific ◽  
Mutational Variance

AbstractAndrodioecious Caenorhabditis have a high frequency of self-compatible hermaphrodites and a low frequency of males. The effects of mutations on male fitness are of interest for two reasons. First, when males are rare, selection on male-specific mutations is less efficient than in hermaphrodites. Second, males may present a larger mutational target than hermaphrodites because of the different ways in which fitness accrues in the two sexes.We report the first estimates of male-specific mutational effects in an androdioecious organism. The rate of male-specific inviable or sterile mutations is ≤ 5 x 10−4/generation, below the rate at which males would be lost solely due to those kinds of mutations. The rate of mutational decay of male competitive fitness is ~0.17%/generation; that of hermaphrodite competitive fitness is ~0.11%/generation. The point estimate of ~1.5X faster rate of mutational decay of male fitness is nearly identical to the same ratio in Drosophila. Estimates of mutational variance (VM) for male mating success and competitive fitness are not significantly different from zero, whereas VM for hermaphrodite competitive fitness is similar to that of non-competitive fitness. The discrepancy between the two sexes is probably due to the greater inherent variability of mating relative to internal self-fertilization.

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