Macroreticulate buffers: a novel approach to pH control in living systems

Advances in molecular biology and biophysics (at molecular-cellular level) do not contribute to understanding of life phenomena. Achievements of synergetics (H. Haken) and complexity theory (I. Prigogine) have intensified differences between physical-chemical understandings of life and systemic understandings. In addition to that, the systems approach provides the understanding of effects of living objects and especially its more organized and evolving part – human and humanity. Human-scaled systems possess the unique property – continuous and chaotic movement of many components of a system state vector x= x(t). Taking this property into consideration causes the denial of any known types of stationary nodes (for example, dx/dt=0) and requires reconsideration of concept of chaos. A novel approach for understanding of living systems (as the third paradigm of natural science) and novel methods of studying of living systems (as theory of chaos and self-organization) are proposed by the third paradigm and theory of chaos and self-organization.

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Levels of Description: A Novel Approach to Dynamical Hierarchies

Artificial Life ◽

10.1162/106454605774270615 ◽

2005 ◽

Vol 11 (4) ◽

pp. 459-472 ◽

Cited By ~ 9

Author(s):

Simon McGregor ◽

Chrisantha Fernando

Keyword(s):

Information Theory ◽

Dynamical Systems ◽

Empirical Studies ◽

State Dependence ◽

Partial Ordering ◽

Living Systems ◽

Novel Approach ◽

Dependence Criterion

We present a novel formal interpretation of dynamical hierarchies based on information theory, in which each level is a near-state-determined system, and levels are related to one another in a partial ordering. This reformulation moves away from previous definitions, which have considered unique hierarchies of structures or objects arranged in aggregates. Instead, we consider hierarchies of dynamical systems: these are more suited to describing living systems, which are not mere aggregates, but organizations. Transformations from lower to higher levels in a hierarchy are redescriptions that lose information. There are two criteria for partial ordering. One is a state-dependence criterion enforcing predictability within a level. The second is a distinctness criterion enforcing the idea that the higher-level description must do more than just throw information away. We hope this will be a useful tool for empirical studies of both computational and physical dynamical hierarchies.

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A novel approach to study the structure-property relationships and applications in living systems of modular Cu2+ fluorescent probes

Scientific Reports ◽

10.1038/srep28972 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 14

Author(s):

Mengyao She ◽

Zheng Yang ◽

Likai Hao ◽

Zhaohui Wang ◽

Tianyou Luo ◽

...

Keyword(s):

Fluorescent Probes ◽

Living Systems ◽

Structure Property ◽

Structure Property Relationships ◽

Novel Approach

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Redox Reactions in Lipid Membranes as a Model for Primordial Energy-Conserving Systems

International Astronomical Union Colloquium ◽

10.1017/s0252921100014950 ◽

1997 ◽

Vol 161 ◽

pp. 437-442

Author(s):

Salvatore Di Bernardo ◽

Romana Fato ◽

Giorgio Lenaz

Keyword(s):

Experimental Evidence ◽

Lipid Membranes ◽

Energy Supply ◽

Redox Reactions ◽

Living Systems ◽

Asymmetric Distribution ◽

Conservation Of Energy ◽

Asymmetrical Distribution ◽

Energy Conserving ◽

Living Organisms

AbstractOne of the peculiar aspects of living systems is the production and conservation of energy. This aspect is provided by specialized organelles, such as the mitochondria and chloroplasts, in developed living organisms. In primordial systems lacking specialized enzymatic complexes the energy supply was probably bound to the generation and maintenance of an asymmetric distribution of charged molecules in compartmentalized systems. On the basis of experimental evidence, we suggest that lipophilic quinones were involved in the generation of this asymmetrical distribution of charges through vectorial redox reactions across lipid membranes.

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Prolonged Fixation Studies For Spaceflight

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072101 ◽

1973 ◽

Vol 31 ◽

pp. 332-333

Author(s):

Robert Corbett ◽

Delbert E. Philpott ◽

Sam Black

Keyword(s):

High Pressure ◽

Living Systems ◽

Prolonged Storage ◽

Time Intervals ◽

Early Signs ◽

Large Numbers

Observation of subtle or early signs of change in spaceflight induced alterations on living systems require precise methods of sampling. In-flight analysis would be preferable but constraints of time, equipment, personnel and cost dictate the necessity for prolonged storage before retrieval. Because of this, various tissues have been stored in fixatives and combinations of fixatives and observed at various time intervals. High pressure and the effect of buffer alone have also been tried.Of the various tissues embedded, muscle, cartilage and liver, liver has been the most extensively studied because it contains large numbers of organelles common to all tissues (Fig. 1).

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Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Biochemical Journal ◽

10.1042/bcj20190591 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3705-3719 ◽

Cited By ~ 2

Author(s):

Avani Vyas ◽

Umamaheswar Duvvuri ◽

Kirill Kiselyov

Keyword(s):

Oxidative Stress ◽

Head And Neck ◽

Transcriptional Activation ◽

Platinum Resistance ◽

Mrna Levels ◽

Strong Positive Correlation ◽

Platinum Compounds ◽

Copper Chelation ◽

Novel Approach ◽

Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

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Collecting Words: A Clinical Example of a Morphology-Focused Orthographic Intervention

Language Speech and Hearing Services in Schools ◽

10.1044/2020_lshss-19-00050 ◽

2020 ◽

Vol 51 (3) ◽

pp. 544-560 ◽

Cited By ~ 3

Author(s):

Kimberly A. Murphy ◽

Emily A. Diehm

Keyword(s):

Written Language ◽

Morphological Knowledge ◽

English Orthography ◽

Word Level ◽

Novel Approach ◽

Language Pathology ◽

The One ◽

Critical Intervention ◽

Reading And Spelling ◽

Reading And Spelling Difficulties

Purpose Morphological interventions promote gains in morphological knowledge and in other oral and written language skills (e.g., phonological awareness, vocabulary, reading, and spelling), yet we have a limited understanding of critical intervention features. In this clinical focus article, we describe a relatively novel approach to teaching morphology that considers its role as the key organizing principle of English orthography. We also present a clinical example of such an intervention delivered during a summer camp at a university speech and hearing clinic. Method Graduate speech-language pathology students provided a 6-week morphology-focused orthographic intervention to children in first through fourth grade ( n = 10) who demonstrated word-level reading and spelling difficulties. The intervention focused children's attention on morphological families, teaching how morphology is interrelated with phonology and etymology in English orthography. Results Comparing pre- and posttest scores, children demonstrated improvement in reading and/or spelling abilities, with the largest gains observed in spelling affixes within polymorphemic words. Children and their caregivers reacted positively to the intervention. Therefore, data from the camp offer preliminary support for teaching morphology within the context of written words, and the intervention appears to be a feasible approach for simultaneously increasing morphological knowledge, reading, and spelling. Conclusion Children with word-level reading and spelling difficulties may benefit from a morphology-focused orthographic intervention, such as the one described here. Research on the approach is warranted, and clinicians are encouraged to explore its possible effectiveness in their practice. Supplemental Material https://doi.org/10.23641/asha.12290687

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