Legume Alternative Oxidase Isoforms Show Differential Sensitivity to Pyruvate Activation

Alternative oxidase (AOX) is an important component of the plant respiratory pathway, enabling a route for electrons that bypasses the energy-conserving, ROS-producing complexes of the mitochondrial electron transport chain. Plants contain numerous isoforms of AOX, classified as either AOX1 or AOX2. AOX1 isoforms have received the most attention due to their importance in stress responses across a wide range of species. However, the propensity for at least one isoform of AOX2 to accumulate to very high levels in photosynthetic tissues of all legumes studied to date, suggests that this isoform has specialized roles, but we know little of its properties. Previous studies with sub-mitochondrial particles of soybean cotyledons and roots indicated that differential expression of GmAOX1, GmAOX2A, and GmAOX2D across tissues might confer different activation kinetics with pyruvate. We have investigated this using recombinantly expressed isoforms of soybean AOX in a previously described bacterial system (Selinski et al., 2016, Physiologia Plantarum 157, 264-279). Pyruvate activation kinetics were similar between the two GmAOX2 isoforms but differed substantially from those of GmAOX1, suggesting that selective expression of AOX1 and 2 could determine the level of AOX activity. However, this alone cannot completely explain the differences seen in sub-mitochondrial particles isolated from different legume tissues and possible reasons for this are discussed.

Inorganic Polymeric Materials Based on Natural Silicate and Aluminosilicate Raw Materials

The prospects of implementing the mechanochemical activation method of volcanic silicate and aluminosilicate rocks - perlites, tuffs, pumice, etc. are being considered for the production of a wide range of building materials using energy-conserving technologies. The thermodynamic and kinetic parameters of interaction in the systems of aluminosilicate – NaOH have been presented, indicating low-temperature sintering of volcanic rocks with sodium hydroxide. According to the degree of activity, the rocks have the following order: perlites, tuffs, obsidian, microcline. Kinetic parameters are presented: concentration, temperature, conversion degree, reaction rate constant, time of complete reaction and product layer thickness.

Degradation of Azo Dyes with Different Functional Groups in Simulated Wastewater by Electrocoagulation

Increasing attention has been paid to the widespread contamination of azo dyes in water bodies globally. These chemicals can present high toxicity, possibly causing severe irritation of the respiratory tract and even carcinogenic effects. The present study focuses on the periodically reverse electrocoagulation (PREC) treatment of two typical azo dyes with different functional groups, involving methyl orange (MO) and alizarin yellow (AY), using Fe-Fe electrodes. Based upon the comparative analysis of three main parameters, including current intensity, pH, and electrolyte, the optimal color removal rates for MO and AY could be achieved at a rate of up to 98.7% and 98.6%, respectively, when the current intensity is set to 0.6 A, the pH is set at 6.0, and the electrolyte is selected as NaCl. An accurate predicted method of response surface methodology (RSM) was established to optimize the PREC process involving the three parameters above. The reaction time was the main influence for both azo dyes, while the condition of PREC treatment for AY simulated wastewater was time-saving and energy conserving. According to the further UV–Vis spectrophotometry analysis throughout the procedure of the PREC process, the removal efficiency for AY was better than that of MO, potentially because hydroxyl groups might donate electrons to iron flocs or electrolyze out hydroxyl free radicals. The present study revealed that the functional groups might pose a vital influence on the removal efficiencies of the PREC treatment for those two azo dyes.

Energy-conserving model of hexagonal pattern in Rayleigh-Bénard convection

We have constructed an energy-conserving sixteen mode dynamical system to model hexagonal pattern in Rayleigh-Bénard convection of Boussinesq fluids with symmetric stress-free thermally conducting boundaries. The model shows stable roll pattern at the onset of convection. Hexagon is found to appear in the system via sausage and (or) stationary rhombus patterns. Both up and down hexagons arise periodically or chaotically with roll, sausage and rhombus patterns. Hexagonal patterns exist for all values of the Prandtl number, 1 ≤ Pr ≤ 5 explored here. However the pattern is more prominent for small Pr and k < kc , where k denotes the wave number. The plot of Nusselt number matches with previous theoretical result. In dissipationless limit, the total energy and the unavailable energy are constants though the kinetic energy, the potential energy and the available energy vary with time. The derived model does not diverge for large values of Rayleigh number Ra.

Construction of a Second-order Six-dimensional Hamiltonian-conserving Scheme

Research has analytically shown that the energy-conserving implicit nonsymplectic scheme of Bacchini, Ripperda, Chen, and Sironi provides a first-order accuracy to numerical solutions of a six-dimensional conservative Hamiltonian system. Because of this, a new second-order energy-conserving implicit scheme is proposed. Numerical simulations of a galactic model hosting a BL Lacertae object and magnetized rotating black hole background support these analytical results. The new method with appropriate time steps is used to explore the effects of varying the parameters on the presence of chaos in the two physical models. Chaos easily occurs in the galactic model as the mass of the nucleus, the internal perturbation parameter, and the anisotropy of the potential of the elliptical galaxy increase. The dynamics of charged particles around the magnetized Kerr spacetime is easily chaotic for larger energies of the particles, smaller initial angular momenta of the particles, and stronger magnetic fields. The chaotic properties are not necessarily weakened when the black-hole spin increases. The new method can be used for any six-dimensional Hamiltonian problems, including globally hyperbolic spacetimes with readily available (3 + 1) split coordinates.

Realization of active metamaterials with odd micropolar elasticity

Materials made from active, living, or robotic components can display emergent properties arising from local sensing and computation. Here, we realize a freestanding active metabeam with piezoelectric elements and electronic feed-forward control that gives rise to an odd micropolar elasticity absent in energy-conserving media. The non-reciprocal odd modulus enables bending and shearing cycles that convert electrical energy into mechanical work, and vice versa. The sign of this elastic modulus is linked to a non-Hermitian topological index that determines the localization of vibrational modes to sample boundaries. At finite frequency, we can also tune the phase angle of the active modulus to produce a direction-dependent bending modulus and control non-Hermitian vibrational properties. Our continuum approach, built on symmetries and conservation laws, could be exploited to design others systems such as synthetic biofilaments and membranes with feed-forward control loops.