An egg-hatch assay for resistance to levamisole in trichostrongyloid nematode parasites

1986 ◽  
Vol 19 (1-2) ◽  
pp. 77-84 ◽  
Author(s):  
R.J. Dobson ◽  
A.D. Donald ◽  
P.J. Waller ◽  
K.L. Snowdon
Keyword(s):  
Egg Hatch ◽  
Nematode Parasites ◽  
Egg Hatch Assay

scholarly journals In vitro Efficacy of Albendazole against Strongyle eggs recovered from Trade Goats slaughtered at the Nsukka Abattoir: A Preliminary Survey of Resistance to Albendazole

2021 ◽  
Vol 40 (4) ◽  
pp. 287-295
Author(s):  
I.K. Idika ◽  
V.J. Ebuk ◽  
E.I. Okoro ◽  
T.A. Nzeakor ◽  
N.M. Uzonnah ◽  
...  
Keyword(s):  
Faecal Sample ◽  
Anthelmintic Resistance ◽  
Egg Hatch ◽  
Lc50 Value ◽  
Nematode Parasites ◽  
Faecal Culture ◽  
World Association ◽  
Egg Hatch Assay ◽  
Set Up

The efficacy of Albendazole against trichostrongyle nematode parasites in goats presented for slaughter at the Nsukka municipal  abattoir was evaluated using the In vitro Egg hatch assay (EHA) model. The abattoir was visited once every week for 4 consecutive months during which a total of 240 goats were sampled. Fecal samples were collected per rectum from a minimum of 15 goats on each day of the visit. Egg Hatch Assay was performed on strongyle eggs recovered from pooled faecal sample on each day of sampling with a 2.5% W/V Albendazole. Faecal culture was also set up from the pooled faecal sample on each sampling day to recover and identify the nematode parasites present in the goats. Among the 240 goats sampled, the prevalence of trichostrongylosis as observed by the presence of strongyle eggs was 94.6% (227/240). Faecal culture and larval identification revealed 69.8% of the strongyles as  Haemonchus contortus, while 25.5 and 4.8% were Trichostrongylus colubriformis and Oesophagostomum species respectively. In the EHA, Albendazole had mean LC50 value of 0.16 µg/ml which is slightly in excess of the discriminating dose of 0.1µg/ml as prescribed by the World Association for the Advancement of Veterinary Parasitology (WAAVP) as an indication of anthelmintic resistance. There is therefore an urgent need to screen the nematode parasite population in the Nigeria for the presence Albendazole resistance genes. Key words: GI nematode; egg hatch assay; goat; Albendazole; Resistance; Nigeria

scholarly journals Bacterial Culture Filtrates Antinematode Activity of Abomasum Bacterial Culture Filtrates against Haemonchus contortus in Small Ruminants

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1843
Author(s):  
Asfa Nazish ◽  
Fozia ◽  
Baharullah Khattak ◽  
Taj Ali Khan ◽  
Ijaz Ahmad ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Bacterial Culture ◽  
Small Ruminants ◽  
Adult Mortality ◽  
Comamonas Testosteroni ◽  
Bacterial Isolates ◽  
Egg Hatch ◽  
Nematode Parasites ◽  
Culture Filtrates ◽  
Nematode Mortality

Haemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. Haemonchus contortus is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes causes the death of the infected animals. The evaluation of the biocontrol potential of some abomasum bacterial isolates against H. contortus is investigated in this study. Out of which, three isolates—Comamonas testosteroni, Comamonas jiangduensis, Pseudomonas weihenstephanesis—show significant effect against the nematode L3, adult, and egg hatch inhibition assays. Various concentrations of metabolites from these bacteria are prepared and applied in different treatments compared with control. In the case of adult mortality assay, 50% metabolites of C. testosteroni and P. weihenstephanesis show 46% adult mortality, whereas C. jiangduensis shows 40% mortality. It is observed that decreasing the concentration of bacterial metabolite, lowers nematode mortality. The minimum nematode mortality rate is recorded at the lowest filtrates concentration of all the bacterial isolates. The same trend is observed in egg hatch inhibition assay, where the higher concentration of bacterial culture filtrates shows 100% inhibition of H. contortus egg. It is concluded that the effect of bacterial culture filtrates against H. contortus is dose-dependent for their activity against nematode L3, adult, and inhibition of egg hatchment.

Development of an egg hatch assay for the detection of anthelmintic resistance to albendazole in Fasciola hepatica isolated from sheep

2014 ◽  
Vol 203 (1-2) ◽  
pp. 217-221 ◽  
Author(s):  
D. Robles-Pérez ◽  
J.M. Martínez-Pérez ◽  
F.A. Rojo-Vázquez ◽  
M. Martínez-Valladares
Keyword(s):  
Fasciola Hepatica ◽  
Anthelmintic Resistance ◽  
Egg Hatch ◽  
Egg Hatch Assay

Evaluation of the Egg Hatch Assay and the Larval Migration Inhibition Assay to detect anthelmintic resistance in cattle parasitic nematodes on farms

2012 ◽  
Vol 61 (4) ◽  
pp. 614-618 ◽  
Author(s):  
Janina Demeler ◽  
Nina Kleinschmidt ◽  
Ursula Küttler ◽  
Regine Koopmann ◽  
Georg von Samson-Himmelstjerna
Keyword(s):  
Anthelmintic Resistance ◽  
Parasitic Nematodes ◽  
Inhibition Assay ◽  
Migration Inhibition ◽  
Egg Hatch ◽  
Larval Migration ◽  
Egg Hatch Assay

Variability of the egg hatch assay to survey benzimidazole resistance in nematodes of small ruminants under field conditions

2014 ◽  
Vol 203 (1-2) ◽  
pp. 102-113 ◽  
Author(s):  
C. Calvete ◽  
L.M. Ferrer ◽  
D. Lacasta ◽  
R. Calavia ◽  
J.J. Ramos ◽  
...  
Keyword(s):  
Small Ruminants ◽  
Field Conditions ◽  
Egg Hatch ◽  
Benzimidazole Resistance ◽  
Egg Hatch Assay

scholarly journals In Vitro Pharmacodynamics of Benzimidazole and Tetrahydropyrimidine Derivatives in European Bison Gastro-Intestinal Nematodes

2017 ◽  
Vol 74 (2) ◽  
pp. 167
Author(s):  
Laura CĂTANĂ ◽  
Aurora URSACHE ◽  
Alexandra PETCU ◽  
Flavia PETREAN ◽  
Raul CĂTANĂ
Keyword(s):  
Larval Development ◽  
Nematode Population ◽  
In Vitro Tests ◽  
European Bison ◽  
Egg Hatch ◽  
Parasitic Resistance ◽  
Faecal Samples ◽  
Egg Hatch Assay ◽  
Inducing Resistance

The present study was aimed to evaluate the efficacy of anthelmintic agents against intestinal nematodes found in European bison. It was performed between October 2016 and May 2017, using Egg Hatch Assay (EHA) and Larval Development Assay (LDA). The parasites were obtained from faecal samples, harvested from bisons in Romania and Sweden. The efficacy of albendazole (ABZ), mebendazole (MBZ) thiabendazole (TBZ) and pyrantel (PYR) was tested. In EHA, the maximum efficacy was observed in MBZ (EC50 = - 0.227 μg/ml), and then TBZ (EC50 = - 0.2228). ABZ had a weaker result, EC50 being 0.326 μg/ml. All tested benzimidazoles registered hatching percentages below 50%, reflecting the lack of parasitic resistance. MIC obtained in the LDA tests were 0.2144 μg/ml for TBZ, 0.2792 μg/ml for PYR, 0.5429 μg/ml for MBZ, while ABZ came last (MIC = 0.8187 μg/ml). The in vitro tests proved the antiparasitic molecules efficacy against bisons nematode population and a limited risk of inducing resistance phenomena.

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