Enzymatic synthesis of ? by a cell-free particulate system of mycobacterium smegmatis

1976 ◽  
Vol 428 (3) ◽  
pp. 563-572 ◽  
Author(s):  
J Schultz
Keyword(s):  
Mycobacterium Smegmatis ◽  
Enzymatic Synthesis ◽  
Particulate System ◽  
A Cell

Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings

1980 ◽  
Vol 35 (3-4) ◽  
pp. 204-208 ◽  
Author(s):  
Dieter Strack
Keyword(s):  
Phenolic Acids ◽  
Enzymatic Synthesis ◽  
Raphanus Sativus ◽  
High Performance ◽  
Sinapic Acid ◽  
Carboxyl Group ◽  
Ph Optimum ◽  
Free System ◽  
Sh Group ◽  
A Cell

Abstract Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group of phenolic acids. Enzymatic activity was determined spectrophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glucosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of dithiothreitol (DTT). The formation of 1-sinapoylglucose was found to be a reversible reaction, since the addition of UDP results in a breakdown of the ester.

scholarly journals OXIDATIVE PHOSPHORYLATION BY A CELL-FREE PARTICULATE SYSTEM FROM UNFERTILIZED ARBACIA EGGS

1950 ◽  
Vol 33 (5) ◽  
pp. 547-553 ◽  
Author(s):  
A. K. Keltch ◽  
C. F. Strittmatter ◽  
C. P. Walters ◽  
G. H. A. Clowes
Keyword(s):  
Oxidative Phosphorylation ◽  
Tricarboxylic Acid Cycle ◽  
High Energy ◽  
Equal Weight ◽  
Maximum Oxygen Consumption ◽  
Arbacia Punctulata ◽  
Particulate System ◽  
Mammalian Liver ◽  
A Cell ◽  
Ph Range

1. A cell-free particulate system capable of effecting oxidative phosphorylation has been prepared from unfertilized eggs of Arbacia punctulata. A substantial increase in phosphorylation can be produced by addition of α-ketoglutarate, oxalacetate, or succinate, the magnitude of the increase being greatest with α-ketoglutarate. The activity of the phosphorylating system is sharply dependent on maintenance of a comparatively narrow pH range during both the preparation of the particulate system and its subsequent incubation with oxidizable substrate. 2. The maximum oxygen consumption of the cell-free particulate system derived from a given weight of unfertilized eggs is about three times that of the same weight of intact unfertilized eggs and approximately the same as that of an equal weight of fertilized eggs. 3. The data indicate that generation of high-energy phosphate bonds in the Arbacia egg is coupled, as it is in mammalian liver or kidney, with the functioning of the tricarboxylic acid cycle.

scholarly journals ACTION OF NITRO- AND HALOPHENOLS UPON OXYGEN CONSUMPTION AND PHOSPHORYLATION BY A CELL-FREE PARTICULATE SYSTEM FROM ARBACIA EGGS

1950 ◽  
Vol 33 (5) ◽  
pp. 555-561 ◽  
Author(s):  
G. H. A. Clowes ◽  
A. K. Keltch ◽  
C. F. Strittmatter ◽  
C. P. Walters
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Oxidative Phosphorylation ◽  
High Energy ◽  
Substituted Phenols ◽  
High Energy Phosphate ◽  
Particulate System ◽  
A Cell ◽  
Phosphate Groups ◽  
Stimulation Of

1. The ability of 4,6-dinitrocresol and eight other substituted phenols to stimulate oxygen uptake and inhibit phosphorylation by a cell-free particulate system from unfertilized Arbacia eggs has been determined. Five of those agents can produce both stimulation of oxygen consumption and inhibition of phosphorylation; one inhibits both oxygen consumption and phosphorylation; and two have no effect on either oxygen consumption or phosphorylation. In every case the effects of these substituted phenols upon the cell-free particulate systems parallel those upon oxygen consumption and cleavage in the intact fertilized Arbacia eggs. 2. The data suggest that energy for cleavage of the Arbacia egg is provided at least in part by oxidative phosphorylation and that substituted phenols may block cleavage by interfering with generation and transfer of high-energy phosphate groups.

scholarly journals Enzymatic Synthesis ofα-Linked Galactooligosaccharides Using the Reverse Reaction of a Cell-boundα-Galactosidase fromCandida guilliermondiiH-404

1995 ◽  
Vol 59 (2) ◽  
pp. 179-183 ◽  
Author(s):  
Hiroyuki Hashimoto ◽  
Chie Katayama ◽  
Masaru Goto ◽  
Tatsuyuki Okinaga ◽  
Sumio Kitahata
Keyword(s):  
Enzymatic Synthesis ◽  
Reverse Reaction ◽  
A Cell
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