The effects of tobramycin, amiloride, sodium cholate and sodium deoxycholate on the growth inhibitory activity of ciprofloxacin against a respiratory tract isolate of Pseudomonas aeruginosa

1994 ◽  
Vol 101 (3) ◽  
pp. R7-R10
Author(s):  
David S. Jones ◽  
Karen N. Shaffer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Respiratory Tract ◽  
Inhibitory Activity ◽  
Sodium Deoxycholate ◽  
Sodium Cholate ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity

scholarly journals SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA PRODUCING ENZYMES AGAINST ANTIMICROBIAL AGENT CELL FREE SUPERNATANT OF LACTOCOCCUS WITH THE FOCUS ON ITS DETERMINING QUANTITATIVELY BY OD (ENZYME LINKED IMMUNE SORBENT ASSAY)

2017 ◽  
Vol 10 (3) ◽  
pp. 291
Author(s):  
Yusra Mb Mohsin ◽  
Huda Zuheir Majeed ◽  
Ali Murtatha Hasan ◽  
Enaam Hamed Bataah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Optical Density ◽  
Inhibitory Activity ◽  
Susceptibility Pattern ◽  
Cell Free Supernatant ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Potential Antimicrobial Activity

ABSTRACTObjective: The present work was conducted to explore the prevalence of Pseudomonas aeruginosa that produce lipase and protease enzymes in40 samples, as well as detection of quality and quantity production and determine its susceptibility to antimicrobial.Methods: The antimicrobial activity of Lactococcus cell free supernatant (CFS) on P. aeruginosa growth and quantity production of enzymes by twomethods (special media and OD) were also studied.Results: A total of 8(20%) and 13(32%) isolates were found to be positive to P. aeruginosa producing lipase and protease, respectively. The in vitroantimicrobial activity results revealed that all isolates producing enzymes showed sensitive to CFS of Lactococcus, only one isolate exhibited lowsensitivity to CFS 4 mm, however, these isolates varied in their sensitivity to CFS ranged 4-15 mm.Conclusion: Results of the quantity production of P. aeruginosa enzymes with CFS of Lactococcus showed and exerts growth inhibitory activity andreduce the production of enzymes. We could be concluded that CFS of Lactococcus has great potential antimicrobial activity against P. aeruginosagrowth and its ability on enzymes production.Keywords: Pseudomonas aeruginosa, Lactococcus, Cell-free supernatant, Optical density, Lipase, Protease.

scholarly journals Modifications on the Tetrahydroquinoline Scaffold Targeting a Phenylalanine Cluster on GPER as Antiproliferative Compounds against Renal, Liver and Pancreatic Cancer Cells

2021 ◽  
Vol 14 (1) ◽  
pp. 49
Author(s):  
David Méndez-Luna ◽  
Loreley Araceli Morelos-Garnica ◽  
Juan Benjamín García-Vázquez ◽  
Martiniano Bello ◽  
Itzia Irene Padilla-Martínez ◽  
...  
Keyword(s):  
Binding Site ◽  
Cell Lines ◽  
Cancer Cell ◽  
Inhibitory Activity ◽  
In Silico ◽  
Cancer Cell Lines ◽  
Pancreatic Cancer Cells ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
New Compounds

The implementation of chemo- and bioinformatics tools is a crucial step in the design of structure-based drugs, enabling the identification of more specific and effective molecules against cancer without side effects. In this study, three new compounds were designed and synthesized with suitable absorption, distribution, metabolism, excretion and toxicity (ADME-tox) properties and high affinity for the G protein-coupled estrogen receptor (GPER) binding site by in silico methods, which correlated with the growth inhibitory activity tested in a cluster of cancer cell lines. Docking and molecular dynamics (MD) simulations accompanied by a molecular mechanics/generalized Born surface area (MMGBSA) approach yielded the binding modes and energetic features of the proposed compounds on GPER. These in silico studies showed that the compounds reached the GPER binding site, establishing interactions with a phenylalanine cluster (F206, F208 and F278) required for GPER molecular recognition of its agonist and antagonist ligands. Finally, a 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay showed growth inhibitory activity of compounds 4, 5 and 7 in three different cancer cell lines—MIA Paca-2, RCC4-VA and Hep G2—at micromolar concentrations. These new molecules with specific chemical modifications of the GPER pharmacophore open up the possibility of generating new compounds capable of reaching the GPER binding site with potential growth inhibitory activities against nonconventional GPER cell models.

Indole-3-carbinol inhibits the proliferation of colorectal carcinoma LoVo cells through activation of the apoptotic signaling pathway

2021 ◽  
pp. 096032712110214
Author(s):  
JY Lee ◽  
HM Lim ◽  
CM Lee ◽  
S-H Park ◽  
MJ Nam
Keyword(s):  
Colorectal Carcinoma ◽  
Cancer Cells ◽  
Inhibitory Activity ◽  
Annexin V ◽  
Fluorescein Isothiocyanate ◽  
Cell Counting ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Lovo Cells

Indole-3-carbinol (I3C) is a phytochemical that exhibits growth-inhibitory activity against various cancer cells. However, there are limited studies on the effects of I3C on colon cancer cells. In this study, the growth-inhibitory activity of I3C against the human colorectal carcinoma cell line (LoVo) was examined. The results of the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide, colony formation, and cell counting assays revealed that I3C suppressed the proliferation of LoVo cells. Microscopy and wound-healing analyses revealed that I3C affected the morphology and inhibited the migration of LoVo cells, respectively. I3C induced apoptosis and DNA fragmentation as evidenced by the results of fluorescein isothiocyanate-conjugated annexin V staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling assay, respectively. Additionally, I3C arrested the cell cycle at the G0/G1 phase and enhanced the reactive oxygen species levels. Western blotting analysis revealed that treatment with I3C resulted in the activation of apoptotic proteins, such as poly(ADP-ribose) polymerase, caspase-3, caspase-7, caspase-9, Bax, Bim, and p53 in LoVo cells. These results indicate that I3C induces apoptosis in LoVo cells by upregulating p53, leading to the activation of Bax and caspases. Taken together, I3C exerts cytotoxic effects on LoVo cells by activating apoptosis.

scholarly journals Structural Determinants of Heparin's Growth Inhibitory Activity

1989 ◽  
Vol 264 (3) ◽  
pp. 1534-1542
Author(s):  
T C Wright ◽  
J J Castellot ◽  
M Petitou ◽  
J C Lormeau ◽  
J Choay ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Structural Determinants ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity

Spontaneous immortalisation of Schwann cells in culture: short-term cultured Schwann cells secrete growth inhibitory activity

Development ◽  
1991 ◽  
Vol 112 (1) ◽  
pp. 33-42
Author(s):  
P.A. Eccleston ◽  
R. Mirsky ◽  
K.R. Jessen
Keyword(s):  
Growth Factor ◽  
Schwann Cell ◽  
Dna Synthesis ◽  
Schwann Cells ◽  
Inhibitory Activity ◽  
Short Term ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Cultured Schwann Cells

In the developing peripheral nerve, Schwann cells proliferate rapidly and then become quiescent, an essential step in control of Schwann cell differentiation. Cell proliferation is controlled by growth factors that can exert positive or inhibitory influences on DNA synthesis. It has been well established that neonatal Schwann cells divide very slowly in culture when separated from neurons but here we show that when culture was continued for several months some cells began to proliferate rapidly and non-clonal lines of immortalised Schwann cells were established which could be passaged for over two years. These cells had a similar molecular phenotype to short-term cultured Schwann cells, except that they expressed intracellular and cell surface fibronectin. The difference in proliferation rates between short- and long-term cultured Schwann cells appeared to be due in part to the secretion by short-term cultured Schwann cells of growth inhibitory activity since DNA synthesis of long-term, immortalised Schwann cells was inhibited by conditioned medium from short-term cultures. This conditioned medium also inhibited DNA synthesis in short-term Schwann cells stimulated to divide by glial growth factor or elevation of intracellular cAMP. The growth inhibitory activity was not detected in the medium of long-term immortalised Schwann cells, epineurial fibroblasts, a Schwannoma (33B), astrocytes or a fibroblast-like cell-line (3T3) and it did not inhibit serum-induced DNA synthesis in epineurial fibroblasts, 33B cells or 3T3 cells. The activity was apparently distinct from transforming growth factor-beta, activin, IL6, epidermal growth factor, atrial natriuretic peptide and gamma-interferon and was heat and acid stable, resistant to collagenase and destroyed by trypsin treatment. We raise the possibility that loss of an inhibitory autocrine loop may contribute to the rapid proliferation of long-term cultured Schwann cells and that an autocrine growth inhibitor may have a role in the cessation of Schwann cell division that precedes differentiation in peripheral nerve development.

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