High-concentration cultivation of lactic acid bacteria in fermentor with cross-flow filtration

1987 ◽  
Vol 65 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Masayuki Taniguchi ◽  
Nobuharu Kotani ◽  
Takeshi Kobayashi
1993 ◽  
Vol 28 (7) ◽  
pp. 9-15 ◽  
Author(s):  
Taro Urase ◽  
Kazuo Yamamoto ◽  
Shinichiro Ohgaki

Virus removal in membrane separation processes was investigated by employing coliphage Qβ as a tracer. Several types of microfiltration membrane and ultrafiltration membrane were tested. Two types of filtration experiments were carried out; dead-end filtration and cross-flow filtration. The membrane surface deposits played an important role in the rejection of viruses in the filtration of activated sludge and pond-water, whereas acrylate polymer cake did not affect the rejection of Qβ. The leakage of ultrafiltration membranes was well examined by the high concentration of Qβ applied. The major part of the rejected coliphages were adsorbed onto the membrane and in its surface deposit.


2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.


1992 ◽  
Vol 25 (10) ◽  
pp. 319-327 ◽  
Author(s):  
P. D. Rose ◽  
B. A. Maart ◽  
T. D. Phillips ◽  
S. L. Tucker ◽  
A. K. Cowan ◽  
...  

An algal high rate oxidation ponding process for treating organic s present in saline effluents has been described. The extreme halophile Dunaliella salina can be made to predominate in the system by manipulating salinity, producing products of value together with a waste treatment function. Application in treating tannery saline organic wastes was examined. Techniques appropriate for the harvesting of micro-algae from this and other algal production systems presents a limiting factor in the development of algal biotechnology. Cross-flow filtration was evaluated as a technique for micro-algal cell separation. Both microfiltration and ultrafiltration were found to produce effective algal removal from the medium, Cross-flow ultrafiltration with a polyethersulfone coated tubular filter produced effective separation with the production of cell concentrates in a viable condition. Flux rates of 30 - 40 LMH fall within acceptable levels for application in industrial processes. Cell shattering observed with microfiltration precludes its use for recovering whole or viable cell concentrates.


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