Role of mesenchymal stem cells in central nervous system regenerative medicine: past, present, and future

Mesenchymal stem cells (MSCs) hold great promise as therapeutic agents in regenerative medicine and autoimmune diseases, based on their differentiation abilities and immunosuppressive properties. However, the therapeutic applications raise a series of questions about the safety of culture-expanded MSCs for human use. This paper summarized recent findings about safety issues of MSCs, in particular their genetic stability in long-termin vitroexpansion, their cryopreservation, banking, and the role of serum in the preparation of MSCs.

Download Full-text

Human Endometrial-Derived Mesenchymal Stem Cells Suppress Inflammation in the Central Nervous System of EAE Mice

Stem Cell Reviews and Reports ◽

10.1007/s12015-011-9338-3 ◽

2011 ◽

Vol 8 (3) ◽

pp. 940-952 ◽

Cited By ~ 30

Author(s):

J. P. S. Peron ◽

T. Jazedje ◽

W. N. Brandão ◽

P. M. Perin ◽

M. Maluf ◽

...

Keyword(s):

Stem Cells ◽

Central Nervous System ◽

Nervous System ◽

Mesenchymal Stem Cells ◽

The Central Nervous System

Download Full-text

Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury

10.1101/239194 ◽

2017 ◽

Cited By ~ 1

Author(s):

Laura N. Zamproni ◽

Marco A.V.M. Grinet ◽

Mayara T.V.V. Mundim ◽

Marcella B.C. Reis ◽

Layla T. Galindo ◽

...

Keyword(s):

Stem Cells ◽

Central Nervous System ◽

Nervous System ◽

Mesenchymal Stem Cells ◽

Mouse Model ◽

Polylactic Acid ◽

Injury Site ◽

Cell Engraftment ◽

Preclinical Trials ◽

The Brain

AbstractTransplanting stem cells into the central nervous system is a promising therapeutic strategy. However, preclinical trials of cell-based therapies are limited by poor local cell engraftment and survival. Here, we present a polylactic acid (PLA) scaffold to support delivery of mesenchymal stem cells (MSCs) in a mouse model of stroke. We isolated bone marrow MSCs from adult C57/Bl6 mice, cultured them on PLA polymeric rough microfibrous (PLA-PRM) scaffolds obtained by rotary jet spinning, and transplanted into the brains of adult C57/Bl6 mice, carrying thermocoagulation-induced cortical stroke. Interleukins (IL4, IL6 and IL10) and tumor necrosis factor alfa (TNFα) expression levels in the brain of mice that received PRM were similar to untreated. MSCs transplantation significantly reduced the area of the lesion and PRM delivery increased MSCs retention at the injury site. We conclude that PLA-PRM scaffolds offer a promising new system to deliver stem cells to injured areas of the brain.GRAPHICAL ABSTRACTSynthetic scaffolds offer an alternative to optimize stem cell transplantation at sites of brain injury. Here, we present a rotary jet spun polylactic acid (PLA) polymer used as a scaffold to support delivery of mesenchymal stem cells (MSCs) in a mouse model of stroke. Transplantation of MSCs isolated or cultured on PRM significantly reduced the area of the lesion and PRM delivery increased MSCs retention at the injury site.

Download Full-text

Bioinformatic Analysis of Transcriptional Regulation by Nur77 in Central Nervous System and Immune System

10.21203/rs.3.rs-505939/v1 ◽

2021 ◽

Author(s):

Montserrat Olivares Costa ◽

Fernando Faunes ◽

María Estela Andrés

Keyword(s):

Stem Cells ◽

Central Nervous System ◽

Nervous System ◽

Transcription Factor ◽

Immune Cells ◽

Binding Sites ◽

Target Genes ◽

Bioinformatic Analysis ◽

Neuronal Stem Cells

Abstract ObjectiveThe objectives of this work were to find genes regulated by Nur77 in neurons and to evaluate the possible common role of this transcription factor in neurons and lymphatic cells using published experimentally generated databases of ChIP-Seq and a microarray. We also characterized Nur77 binding throughout the genome. ResultsWe identified 113 Nur77 target genes in neuronal stem cells and 116 in neuronal cells. Cell adhesion and anchoring processes emerged as regulated by Nur77 in neurons and lymphatic cells. We found 9 common genes regulated by Nur77. Finally, we described a significant distribution of Nur77 binding sites in strong enhancers and active promoters. This work is a first step to understand the role of Nur77 and its common targets in neurons and immune cells.

Download Full-text