Economic and political aspects of Dengue virus disease

2020 ◽  
pp. 151-158 ◽  
Author(s):  
Mushtaq H. Qureshi
Keyword(s):  
Dengue Virus ◽  
Virus Disease

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals Bacterial coinfections in dengue virus disease: what we know and what is still obscure about an emerging concern

Infection ◽  
2016 ◽  
Vol 45 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Mattia Trunfio ◽  
Alessia Savoldi ◽  
Ottavia Viganò ◽  
Antonella d’Arminio Monforte
Keyword(s):  
Dengue Virus ◽  
Virus Disease

scholarly journals Molecular detection of dengue virus in patients suspected of Ebola virus disease in Ghana

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208907 ◽  
Author(s):  
Joseph Humphrey Kofi Bonney ◽  
Takaya Hayashi ◽  
Samuel Dadzie ◽  
Esinam Agbosu ◽  
Deborah Pratt ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Ebola Virus ◽  
Molecular Detection ◽  
Virus Disease ◽  
Ebola Virus Disease

scholarly journals The dengue virus non-structural protein 1 (NS1) use the scavenger receptor B1 as cell receptor in human hepatic and mosquito cells

2019 ◽  
Author(s):  
Ana C. Alcalá ◽  
José L. Maravillas ◽  
David Meza ◽  
Octavio T. Ramirez ◽  
Juan E. Ludert ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Disease ◽  
Scavenger Receptor ◽  
Direct Interaction ◽  
Cell Receptor ◽  
Serum Lipoprotein ◽  
Structural Protein ◽  
Surface Binding

AbstractDengue is the most common virus disease transmitted to humans by mosquitoes. The dengue virus NS1 is a multifunctional protein that form part of replication complexes. In addition, NS1 is also secreted, as a hexamer, to the extracellular milieu. Circulating NS1 has been associated with dengue pathogenesis by several different mechanisms. Cell binding and internalization of soluble NS1 result in the disruption of tight junctions and in down regulation of the innate immune response. In this work, we report that the HDL scavenger receptor B1 (SRB1) in human hepatic cells, and a scavenger receptor B1-like in mosquito C6/36 cells act as cell surface binding receptor for dengue virus NS1. The presence of the SRB1 on the plasma membrane of C6/36 cells, as well as in Huh-7 cells, was demonstrated by confocal microcopy. Internalization of NS1 can be efficiently blocked by anti-SRB1 antibodies and previous incubation of the cells with HDL significantly reduces NS1 internalization. In addition, the transient expression of SRB1 in Vero cells, which lack the receptor, renders these cells susceptible to NS1 entry. Direct interaction between soluble NS1 and the SRB1 in Huh7 and C6/36 cells was demonstrated in vivo by proximity ligation assays an in vitro by surface plasmon resonance. Finally, data is presented indicating that the SRB1 also act as cell receptor for zika virus NS1. These results demonstrate that dengue virus NS1, a bona fide lipoprotein, usurps the HDL receptor for cell entry and offers explanations for the altered serum lipoprotein homeostasis observed in dengue patients.

A rare ability to enhance dengue virus disease

Science ◽  
2017 ◽  
Vol 355 (6323) ◽  
pp. 363.8-364
Author(s):  
Caroline Ash
Keyword(s):  
Dengue Virus ◽  
Virus Disease

scholarly journals PO 8559 ASSESSMENT OF THE EFFECT OF RVSV REPLICATION ON NON-EBOLA CIRCULATING ANTIBODIES IN RVSV-ZEBOV-GP-VACCINATED INDIVIDUALS IN LAMBARÉNÉ, GABON

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A53.1-A53
Author(s):  
Armel V Ndong Mbouna ◽  
Selidji T Agnandji
Keyword(s):  
Dengue Virus ◽  
Yellow Fever ◽  
Ebola Virus ◽  
Viral Infections ◽  
Yellow Fever Virus ◽  
Virus Disease ◽  
Fever Virus ◽  
Serum Samples ◽  
Elisa Technique ◽  
Circulating Antibodies

BackgroundThe West Africa Ebola virus disease (EVD) outbreak between 2015 and 2016 accelerated the need for safe and effective vaccines. Among candidate vaccines in clinical development, the recombinant Vesicular stomatitis virus (VSV) vectored with the Ebola virus (EBOV) glycoprotein (rVSV-ZEBOV-GP) vaccine showed acceptable safety and promising immunogenicity results across diverse settings.Baseline screening data from the phase I trial of this vaccine in Lambaréné, Gabon, established that prior to vaccination about 21% (33/155) and 8% (12/155) of adults had naturally acquired antibodies to infectious ZEBOV particle and ZEBOV-GP, respectively. In participants with prior ZEBOV(-GP) antibodies, post-vaccination antibodies titres were significantly higher 56 days following vaccination with doses of 3×103, 3×104, and 3×106 PFU compared to those without.Our study seeks to investigate rVSV vector non-specific boosting of naturally acquired antibodies to other viral infections (dengue virus 1–4, and yellow fever virus).MethodsWe measured antibodies titres to Dengue (serotypes 1–4) and yellow fever infection at baseline, 28 and 56 days after injection in a total of 155 serum samples from vaccinees receiving various doses of rVSV-ZEBOV-GP using ELISA technique.ResultsPreliminary results were presented at the meeting.ConclusionOur results confirm rVSV vector non-specific replication on non ZEBOV-GP circulating antibodies in Lambaréné vaccinees and potential boosting action on naturally acquired dengue virus (serotypes 1–4) and yellow fever virus antibodies.

scholarly journals Dengue Virus Nonstructural Protein NS5 Induces Interleukin-8 Transcription and Secretion

2005 ◽  
Vol 79 (17) ◽  
pp. 11053-11061 ◽  
Author(s):  
Carey L. Medin ◽  
Katherine A. Fitzgerald ◽  
Alan L. Rothman
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Virus Disease ◽  
Nonstructural Protein ◽  
Interleukin 8 ◽  
Hek293 Cells ◽  
Poly I:C ◽  
Target Cells ◽  
Mrna Levels ◽  
Chemokine Production

ABSTRACT Elevated circulating levels of chemokines have been reported in patients with dengue fever and are proposed to contribute to the pathogenesis of dengue disease. To establish in vitro models for chemokine induction by dengue 2 virus (DEN2V), we studied a variety of human cell lines and primary cells. DEN2V infection of HepG2 and primary dendritic cells induced the production of interleukin-8 (IL-8), RANTES, MIP-1α, and MIP-1β, whereas only IL-8 and RANTES were induced following dengue virus infection of HEK293 cells. Chemokine secretion was accompanied by an increase in steady-state mRNA levels. No chemokine induction was observed in HEK293 cells treated with poly(I:C) or alpha interferon, suggesting a direct effect of virus infection. To determine the mechanism(s) involved in the induction of chemokine production by DEN2V, individual dengue virus genes were cloned into plasmids and expressed in HEK293 cells. Transfection of a plasmid expressing NS5 or a dengue virus replicon induced IL-8 gene expression and secretion. RANTES expression was not induced under these conditions, however. Reporter assays showed that IL-8 induction by NS5 was principally through CAAT/enhancer binding protein, whereas DEN2V infection also induced NF-κB. These results indicate a role for the dengue virus NS5 protein in the induction of IL-8 by DEN2V infection. Recruitment and activation of potential target cells to sites of DEN2V replication by virus-induced chemokine production may contribute to viral replication as well as to the inflammatory components of dengue virus disease.

scholarly journals Possible re-infection of SARS-CoV-2 complicated by dengue virus co-infection: report of a rare case from Bangladesh

2020 ◽  
pp. 105-106
Author(s):  
Miah Wahiduzzaman ◽  
Muhammad Abdur Rahim
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Dengue Virus ◽  
Rare Case ◽  
Virus Disease ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Mild Form ◽  
Chain Reaction ◽  
Polymerase Chain

Re-infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and co-infection by dengue virus and SARS-CoV-2 are possible. We report a case of dengue haemorrhagic fever, occurring in a young Bangladeshi man, who concurrently tested positive for SARS-CoV-2 infection by reverse transcriptase polymerase chain reaction (RT-PCR). Four months previously, he suffered a mild form of corona virus disease 2019 (COVID-19). This case is reported to make the physicians aware that, co-infections are possible in this COVID-19 pandemic, specially in dengue endemic regions and countries like Bangladesh. Birdem Med J 2020; 10, COVID Supplement: 105-106

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