PO 8559 ASSESSMENT OF THE EFFECT OF RVSV REPLICATION ON NON-EBOLA CIRCULATING ANTIBODIES IN RVSV-ZEBOV-GP-VACCINATED INDIVIDUALS IN LAMBARÉNÉ, GABON

BackgroundThe West Africa Ebola virus disease (EVD) outbreak between 2015 and 2016 accelerated the need for safe and effective vaccines. Among candidate vaccines in clinical development, the recombinant Vesicular stomatitis virus (VSV) vectored with the Ebola virus (EBOV) glycoprotein (rVSV-ZEBOV-GP) vaccine showed acceptable safety and promising immunogenicity results across diverse settings.Baseline screening data from the phase I trial of this vaccine in Lambaréné, Gabon, established that prior to vaccination about 21% (33/155) and 8% (12/155) of adults had naturally acquired antibodies to infectious ZEBOV particle and ZEBOV-GP, respectively. In participants with prior ZEBOV(-GP) antibodies, post-vaccination antibodies titres were significantly higher 56 days following vaccination with doses of 3×103, 3×104, and 3×106 PFU compared to those without.Our study seeks to investigate rVSV vector non-specific boosting of naturally acquired antibodies to other viral infections (dengue virus 1–4, and yellow fever virus).MethodsWe measured antibodies titres to Dengue (serotypes 1–4) and yellow fever infection at baseline, 28 and 56 days after injection in a total of 155 serum samples from vaccinees receiving various doses of rVSV-ZEBOV-GP using ELISA technique.ResultsPreliminary results were presented at the meeting.ConclusionOur results confirm rVSV vector non-specific replication on non ZEBOV-GP circulating antibodies in Lambaréné vaccinees and potential boosting action on naturally acquired dengue virus (serotypes 1–4) and yellow fever virus antibodies.

Download Full-text

Yellow Fever Virus Exhibits Slower Evolutionary Dynamics than Dengue Virus

Journal of Virology ◽

10.1128/jvi.01738-09 ◽

2009 ◽

Vol 84 (2) ◽

pp. 765-772 ◽

Cited By ~ 46

Author(s):

Amadou A. Sall ◽

Ousmane Faye ◽

Mawlouth Diallo ◽

Cadhla Firth ◽

Andrew Kitchen ◽

...

Keyword(s):

Dengue Virus ◽

Yellow Fever ◽

Central African Republic ◽

Evolutionary Dynamics ◽

Nucleotide Substitution ◽

Viral Infections ◽

Yellow Fever Virus ◽

Fever Virus ◽

Limited Information ◽

Reduced Rate

ABSTRACT Although yellow fever has historically been one of the most important viral infections of humans, relatively little is known about the evolutionary processes that shape its genetic diversity. Similarly, there is limited information on the molecular epidemiology of yellow fever virus (YFV) in Africa even though it most likely first emerged on this continent. Through an analysis of complete E gene sequences, including a newly acquired viral collection from Central and West Africa (Senegal, Cameroon, Central African Republic, Côte d'Ivoire, Mali, and Mauritania), we show that YFV exhibits markedly lower rates of evolutionary change than dengue virus, despite numerous biological similarities between these two viruses. From this observation, along with a lack of clock-like evolutionary behavior in YFV, we suggest that vertical transmission, itself characterized by lower replication rates, may play an important role in the evolution of YFV in its enzootic setting. Despite a reduced rate of nucleotide substitution, phylogenetic patterns and estimates of times to common ancestry in YFV still accord well with the dual histories of colonialism and the slave trade, with areas of sylvatic transmission (such as Kedougou, Senegal) acting as enzootic/epidemic foci.

Download Full-text

Zika Virus: A Clinical Review

KYAMC Journal ◽

10.3329/kyamcj.v7i1.33766 ◽

2017 ◽

Vol 7 (1) ◽

pp. 719-725

Author(s):

Md Daharul Islam ◽

SM Tajdit Rahman ◽

Khaleda Akhter ◽

Md Azizul Hoque ◽

Anannya Roy ◽

...

Keyword(s):

Yellow Fever ◽

Zika Virus ◽

Yellow Fever Virus ◽

Virus Disease ◽

Specific Treatment ◽

Abrupt Onset ◽

Fever Virus ◽

Control Measures ◽

Disease Treatment ◽

Antibodies Detection

Zika virus is a flavivirus related to Dengue virus, yellow fever virus and West Nile virus. It is considered an emerging arbovirus transmitted by mosquito of the genus Aedes. Its first description took place in 1947 in the Zika Forest in Uganda, isolated on Rhesus monkey used as bait to study the yellow fever virus. Clinical picture is characterized as a 'dengue-like' syndrome, with abrupt onset of fever; and an early onset of evanescent rash, often pruritic. Occasionally the disease has been associated with Guillain-Barré syndrome. The diagnosis can be performed by PCR or by IgG and IgM antibodies detection. No specific treatment or vaccine is available for Zika virus disease. Treatment is generally supportive. Control measures are same for dengue and chikungunya based mostly on health education and vector control.KYAMC Journal Vol. 7, No.-1, Jul 2016, Page 719-725

Download Full-text

Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus

Journal of Virological Methods ◽

10.1016/j.jviromet.2019.03.014 ◽

2019 ◽

Vol 268 ◽

pp. 53-55 ◽

Cited By ~ 3

Author(s):

José A. Boga ◽

Marta E. Alvarez-Arguelles ◽

Susana Rojo-Alba ◽

Mercedes Rodríguez ◽

María de Oña ◽

...

Keyword(s):

West Nile Virus ◽

Dengue Virus ◽

Yellow Fever ◽

Zika Virus ◽

Chikungunya Virus ◽

Yellow Fever Virus ◽

Simultaneous Detection ◽

Fever Virus ◽

West Nile ◽

Virus Zika

Download Full-text

Yellow Fever Virus/Dengue-2 Virus and Yellow Fever Virus/Dengue-4 Virus Chimeras: Biological Characterization, Immunogenicity, and Protection against Dengue Encephalitis in the Mouse Model

Journal of Virology ◽

10.1128/jvi.77.6.3655-3668.2003 ◽

2003 ◽

Vol 77 (6) ◽

pp. 3655-3668 ◽

Cited By ~ 33

Author(s):

Thomas J. Chambers ◽

Yan Liang ◽

Deborah A. Droll ◽

Jacob J. Schlesinger ◽

Andrew D. Davidson ◽

...

Keyword(s):

Mouse Model ◽

Dengue Virus ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus ◽

E Proteins ◽

Dengue Viruses ◽

Intracerebral Inoculation ◽

Intraperitoneal Route ◽

Chimeric Viruses

ABSTRACT Two yellow fever virus (YFV)/dengue virus chimeras which encode the prM and E proteins of either dengue virus serotype 2 (dengue-2 virus) or dengue-4 virus within the genome of the YFV 17D strain (YF5.2iv infectious clone) were constructed and characterized for their properties in cell culture and as experimental vaccines in mice. The prM and E proteins appeared to be properly processed and glycosylated, and in plaque reduction neutralization tests and other assays of antigenic specificity, the E proteins exhibited profiles which resembled those of the homologous dengue virus serotypes. Both chimeric viruses replicated in cell lines of vertebrate and mosquito origin to levels comparable to those of homologous dengue viruses but less efficiently than the YF5.2iv parent. YFV/dengue-4 virus, but not YFV/dengue-2 virus, was neurovirulent for 3-week-old mice by intracerebral inoculation; however, both viruses were attenuated when administered by the intraperitoneal route in mice of that age. Single-dose inoculation of either chimeric virus at a dose of 105 PFU by the intraperitoneal route induced detectable levels of neutralizing antibodies against the homologous dengue virus strains. Mice which had been immunized in this manner were fully protected from challenge with homologous neurovirulent dengue viruses by intracerebral inoculation compared to unimmunized mice. Protection was associated with significant increases in geometric mean titers of neutralizing antibody compared to those for unimmunized mice. These data indicate that YFV/dengue virus chimeras elicit antibodies which represent protective memory responses in the mouse model of dengue encephalitis. The levels of neurovirulence and immunogenicity of the chimeric viruses in mice correlate with the degree of adaptation of the dengue virus strain to mice. This study supports ongoing investigations concerning the use of this technology for development of a live attenuated viral vaccine against dengue viruses.

Download Full-text

Fever versus fever: The role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus

Infection Genetics and Evolution ◽

10.1016/j.meegid.2013.03.008 ◽

2013 ◽

Vol 19 ◽

pp. 292-311 ◽

Cited By ~ 98

Author(s):

Kathryn A. Hanley ◽

Thomas P. Monath ◽

Scott C. Weaver ◽

Shannan L. Rossi ◽

Rebecca L. Richman ◽

...

Keyword(s):

Dengue Virus ◽

Interspecific Competition ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus

Download Full-text

High Fidelity of Yellow Fever Virus RNA Polymerase

Journal of Virology ◽

10.1128/jvi.78.2.1032-1038.2004 ◽

2004 ◽

Vol 78 (2) ◽

pp. 1032-1038 ◽

Cited By ~ 82

Author(s):

Konstantin V. Pugachev ◽

Farshad Guirakhoo ◽

Simeon W. Ocran ◽

Fred Mitchell ◽

Megan Parsons ◽

...

Keyword(s):

Dengue Virus ◽

Rna Polymerase ◽

Yellow Fever ◽

Error Rate ◽

Yellow Fever Virus ◽

Protein A ◽

Fever Virus ◽

Genome Replication ◽

Virus Rna

ABSTRACT Three consecutive plaque purifications of four chimeric yellow fever virus-dengue virus (ChimeriVax-DEN) vaccine candidates against dengue virus types 1 to 4 were performed. The genome of each candidate was sequenced by the consensus approach after plaque purification and additional passages in cell culture. Our data suggest that the nucleotide sequence error rate for SP6 RNA polymerase used in the in vitro transcription step to initiate virus replication was as high as 1.34 × 10−4 per copied nucleotide and that the error rate of the yellow fever virus RNA polymerase employed by the chimeras for genome replication in infected cells was as low as 1.9 × 10−7 to 2.3 × 10−7. Clustering of beneficial mutations that accumulated after multiple virus passages suggests that the N-terminal part of the prM protein, a specific site in the middle of the E protein, and the NS4B protein may be essential for nucleocapsid-envelope interaction during flavivirus assembly.

Download Full-text

Steroidal saponins from the roots of Solanum sisymbriifolium Lam. (Solanaceae) have inhibitory activity against dengue virus and yellow fever virus

Brazilian Journal of Medical and Biological Research ◽

10.1590/1414-431x2020e10240 ◽

2021 ◽

Vol 54 (7) ◽

Author(s):

G.G. Figueiredo ◽

O.A. Coronel ◽

A.C. Trabuco ◽

D.E. Bazán ◽

R.R. Russo ◽

...

Keyword(s):

Dengue Virus ◽

Yellow Fever ◽

Inhibitory Activity ◽

Yellow Fever Virus ◽

Fever Virus ◽

Steroidal Saponins ◽

Solanum Sisymbriifolium

Download Full-text

Dengue Virus and Yellow Fever Virus Damage the Liver: A Systematic Review About the Histopathological Profiles

Journal of Gastroenterology and Hepatology Research ◽

10.17554/j.issn.2224-3992.2019.07.823 ◽

2019 ◽

Vol 8 (2) ◽

pp. 2864-2870 ◽

Cited By ~ 1

Author(s):

Dacylla Sampaio Costa ◽

◽

Lucas Arruda Moita ◽

Even Herlany Pereira Alves ◽

Ana Clara Silva Sales ◽

...

Keyword(s):

Systematic Review ◽

Dengue Virus ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus

Download Full-text

Experience of application of IgY-technology for laboratory diagnostics of viral infections

Voprosy Virusologii ◽

10.36233/0507-4088-2020-65-1-21-26 ◽

2020 ◽

Vol 65 (1) ◽

pp. 21-26 ◽

Cited By ~ 2

Author(s):

A. P. Ivanov ◽

T. D. Klebleeva ◽

O. E. Ivanova

Keyword(s):

Yellow Fever ◽

Viral Infections ◽

Yellow Fever Virus ◽

Laboratory Diagnosis ◽

Encephalitis Virus ◽

Fever Virus ◽

Laboratory Animals ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus

Introduction. The well-known advantages of class Y antibodies (IgY) from egg yolks of immunized hens in comparison with class G antibodies (IgG) of laboratory animals traditionally used in laboratory diagnosis of infectious diseases determine the stable interest of researchers in using IgY for these purposes (IgY technology) . Over the past 20 years, the obvious benefits of IgY technology have been demonstrated for a number of viral and bacterial infections. Goals and objectives. Construction of ELISA systems based on specific IgY for laboratory diagnosis of infections caused by tick-borne encephalitis virus, yellow fever virus, poliovirus.Material and methods. Obtaining yolk preparations of immunized chickens, obtaining highly purified IgY preparations (salting out, affinity chromatography), constructing ELISA systems for determining virus-specific antigens, testing the parameters of ELISA systems.Results and discussion. For the first time in laboratory practice, ELISA systems based on the use of specific polyclonal IgY were designed for laboratory diagnosis of topical human viral infections caused by flaviviruses and enteroviruses: determination of antigens of tick-borne encephalitis virus, yellow fever virus, 3 types of poliovirus. It was experimentally shown that these ELISA systems have high sensitivity and specificity, which allows them to be used for the semiquantitative and quantitative determination of antigens of these viruses in various materials (infected cell cultures, vaccines, etc.).Conclusion. The ELISA systems developed on the basis of specific IgY for determination of viral antigens can be effectively used for laboratory diagnosis of a number of viral infections, for the validation and control of vaccine preparations.

Download Full-text