Peritoneal fluid, lymph nodes, masses, peritoneal cavity, and great vessel thrombosis

Abstract Background Endometriosis is a refractory and recurrent disease and it affects nearly 10% of reproductive-aged women and 40% of infertile patients. The commonly accepted theory for endometriosis is retrograde menstruation where endometrial tissues invade into peritoneal cavity and fail to be cleared due to immune dysfunction. Therefore, the comprehensive understanding of immunologic microenvironment of peritoneal cavity deserves further investigation for the previous studies mainly focus on one or several immune cells. Results High-quality transcriptomes were from peritoneal fluid samples of patients with endometriosis and control, and firstly subjected to 10 × genomics single-cell RNA-sequencing. We acquired the single-cell transcriptomes of 10,280 cells from endometriosis sample and 7250 cells from control sample with an average of approximately 63,000 reads per cell. A comprehensive map of overall cells in peritoneal fluid was first exhibited. We unveiled the heterogeneity of immune cells and discovered new cell subtypes including T cell receptor positive (TCR+) macrophages, proliferating macrophages and natural killer dendritic cells in peritoneal fluid, which was further verified by double immunofluorescence staining and flow cytometry. Pseudo-time analysis showed that the response of macrophages to the menstrual debris might follow the certain differentiation trajectory after endometrial tissues invaded into the peritoneal cavity, that is, from antigen presentation to pro-inflammation, then to chemotaxis and phagocytosis. Our analyses also mirrored the dysfunctions of immune cells including decreased phagocytosis and cytotoxic activity and elevated pro-inflammatory and chemotactic effects in endometriosis. Conclusion TCR+ macrophages, proliferating macrophages and natural killer dendritic cells are firstly reported in human peritoneal fluid. Our results also revealed that immune dysfunction happens in peritoneal fluid of endometriosis, which may be responsible for the residues of invaded menstrual debris. It provided a large-scale and high-dimensional characterization of peritoneal microenvironment and offered a useful resource for future development of immunotherapy.

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Met-Enkephalin Involvement in Morphine-Modulated Peritonitis in Swiss Mice

Mediators of Inflammation ◽

10.1155/mi.2005.112 ◽

2005 ◽

Vol 2005 (2) ◽

pp. 112-117 ◽

Cited By ~ 3

Author(s):

Magdalena Chadzinska ◽

Anna Scislowska-Czarnecka ◽

Krystyna Pierzchala-Koziec ◽

Barbara Plytycz

Keyword(s):

Pituitary Gland ◽

Peritoneal Cavity ◽

Peritoneal Fluid ◽

Endogenous Opioids ◽

Time Points ◽

Swiss Mice ◽

Leukocyte Accumulation ◽

Peritoneal Leukocytes

Morphine coinjection with zymosan inhibits pain and leukocyte accumulation during peritonitis in several strains of mice, and affects systems of endogenous opioids. Present investigations focus on Met-enkephalin (Met-ENK) in the inflamed peritoneal cavity and brain centers of Swiss mice. Males of Swiss mice were IP injected with zymosan or zymosan supplemented with morphine. At the selected time points the peritoneal leukocytes were counted and the Met-ENK level was measured in exudatory fluid and leukocytes, striatum, hypothalamus, and pituitary gland. The Met-ENK content in peritoneal fluid rised sharply after zymosan injection, which corresponded with its decline in exudatory leukocytes, hypothalamus, and striatum. Morphine coinjection enhanced intraperitoneal accumulation of Met-ENK and its release from exudatory leukocytes, but inhibited its early fluctuations in hypothalamus and striatum. Effects of morphine-modulated inflammation on the Met-ENK system lasted longer than 7 days.

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Characterization of an intraperitoneal ovarian cancer xenograft model in nude rats using noninvasive microPET imaging

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2007.00814.x ◽

2007 ◽

Vol 17 (2) ◽

pp. 407-417 ◽

Cited By ~ 6

Author(s):

C. L. Zavaleta ◽

W. T. Phillips ◽

Y. C. Bradley ◽

L. M. McMANUS ◽

P. A. Jerabek ◽

...

Keyword(s):

Ovarian Cancer ◽

Lymph Nodes ◽

Peritoneal Cavity ◽

Imaging Modality ◽

Xenograft Model ◽

Tumor Model ◽

Fdg Uptake ◽

Tumor Bearing ◽

Nude Rats ◽

Biodistribution Data

MicroPET is a noninvasive imaging modality that can potentially track tumor development in nude rats using the radiotracer fluorine 18-fluorodeoxyglucose (18F-FDG). Our goal was to determine whether microPET, as opposed to more invasive techniques, could be used to noninvasively monitor the development of ovarian cancer in the peritoneal cavity of nude rats for monitoring treatment response in future studies. Female nude rats were inoculated intraperitoneally with 36 million NIH:OVCAR-3 cells. Imaging was carried out at 2, 4, 6, or 8 weeks postinoculation. Each rat was fasted overnight and intravenously injected with 11.1 MBq (300 μCi) of 18F-FDG in 0.2 mL of saline. Thirty minutes following injection, the rats were placed in the microPET and scanned for 30 min. After imaging, rats were euthanized for ascites and tissue collection for biodistribution and histopathologic correlation. Standard uptake values (SUVs) of 18F-FDG within the peritoneal cavity were also calculated from regions of interest analysis of the microPET images. MicroPET images showed diffuse increased uptake of 18F-FDG throughout the peritoneal cavity of tumor rats (mean SUV = 4.64) compared with control rats (mean SUV = 1.03). Ascites gathered from tumor-bearing rats had increased 18F-FDG uptake as opposed to the peritoneal fluid collected from control rats. Biodistribution data revealed that the percent injected dose per gram (% ID/g) was significantly higher in tumor-bearing rats (6.29%) than in control rats (0.59%) in the peritoneal lymph nodes. Pathology verified that these lymph nodes were more reactive in tumor-bearing rats. By 6 weeks, some rats developed solid masses within the peritoneum, which could be detected on microPET images and confirmed as tumor by histopathology. 18F-FDG uptake in these tumors at necropsy was 2.83% ID/g. These results correlate with previous invasive laparoscopic studies of the same tumor model and demonstrate that microPET using 18F-FDG is a promising noninvasive tool to localize and follow tumor growth in an intraperitoneal ovarian cancer model.

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Peritoneal Fluid and Solute Transport with Different Polyglucose Formulations

Peritoneal Dialysis International ◽

10.1177/089686089801800209 ◽

1998 ◽

Vol 18 (2) ◽

pp. 193-203 ◽

Cited By ~ 18

Author(s):

Tao Wang ◽

Olof Heimbürger ◽

Hui-Hong Cheng ◽

Jonas Bergström ◽

Bengt Lindholm

Keyword(s):

Solute Transport ◽

Peritoneal Cavity ◽

Peritoneal Fluid ◽

Absorption Rate ◽

Glucose Solution ◽

Ex Vivo ◽

Elimination Rate ◽

Fluid Absorption ◽

Significant Difference ◽

Glucose Polymer

Objective To study peritoneal fluid and solute transport characteristics using different polyglucose solutions with and without the addition of glucose. Design Thirty-one rats were divided into three groups. A 4-hour dwell study with frequent dialysate and blood samples was performed in each rat using 25 mL of 7.5% polyglucose solution (PG, n = 11),7.5% polyglucose + 0.35% glucose solution (PG1, n = 12), or 3.75% polyglucose + 1.93% glucose solution (PG2, n = 8). Radiolabeled human albumin (RISA) was added to the solutions as an intraperitoneal volume (IPV) marker. In addition, polyglucose degradation was evaluated ex vivo over 24 hours. Experimental Animals Thirty-one male Sprague Dawley rats (300 g) were used. Main Outcome Measures Fluid and solute (glucose, urea, sodium, potassium, and total protein) transport characteristics as well as changes in dialysate osmolality were evaluated. Results The IPV was higher in the PG1 and PG2 groups than in the PG group during the first 2 hours of the dwell. The IPV, in fact, decreased during the first hour of the dwell in the PG group. However, the net ultrafiltration at 4 hours tended to be lower in the PG2 (3.2 ± 1.5 mL) group compared to the PG (5.1 ± 2.3 mL) and the PG1 groups (5.2 ± 2.1 mL) (p = 0.07), and no significant difference was found between the PG and PG1 groups. Adding glucose to the PG solution increased the RISA elimination rate (KE, representing the fluid absorption rate from the peritoneal cavity): 25.5 ± 8.2, 37.5 ± 12.2, and 42.5 ± 8.9 μL/ min for the PG, PG1, and the PG2 group, respectively, p < 0.01. Dialysate osmolality (Dos) increased with the dwell time in the PG and PG1 groups but decreased in the PG2 group. The increase in Dos was partially due to the degradation of glucose polymer, which was supported by the marked increase in osmolality over 24 hours of incubation of PG solution with peritoneal fluid, ex vivo. The diffusive mass transport coefficient for the investigated solutes did not differ among the three groups (except for glucose, which was significantly lower in the PG group). The sieving coefficient for sodium was significantly higher in the PG group compared to the PG1 group (p < 0.05). Conclusion Our results suggest that, although there was an initial decrease in the intraperitoneal dialysate volume, significant amounts of fluid can be removed by polyglucose solution during a single 4-hour dwell in rats, despite the low osmolality of the solution. The positive fluid removal induced by the PG solution is partially due to the lower fluid absorption rate associated with this solution and may, to some extent, also be due to the degradation of glucose polymer within the peritoneal cavity, resulting in increased dialysate osmolality. The addition of glucose to the polyglucose solution does not seem to improve ultrafiltration in a 4-hour dwell in the rat model. However, the peritoneal fluid absorption rate may be increased, and peritoneal transport of glucose and sodium may be altered, by adding glucose to the polyglucose solution.

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The fate of isotope-labelled uterine spermatozoa in the mouse post coitum

Australian Journal of Zoology ◽

10.1071/zo9650525 ◽

1965 ◽

Vol 13 (4) ◽

pp. 525 ◽

Cited By ~ 15

Author(s):

BL Reid

Keyword(s):

Lymph Nodes ◽

Peritoneal Cavity ◽

Tritiated Thymidine ◽

Uterine Wall ◽

Phagocytic Cells ◽

Uterine Lumen ◽

Polymorphonuclear Granulocytes ◽

Uterine Mucosa ◽

Needle Track ◽

Degree Of Degradation

Isotopically labelled sperm was used to investigate the fate of the uterine sperm residue not used in the process of fertilization of the mouse. Portion of the sperm present in the uterus exhibiting a copulation plug was removed and replaced by intrauterine injection of sperm labelled either specifically by tritiated thymidine or non-specifically by exposure to a tritium source. The latter label was found more suitable for tracer use although the results with both methods were qualitatively similar. Seventeen hours after injection label was present in sperm in the lumen, in debris associated with polymorphonuclear granulocytes and monocytes in the lumen, in the epithelial and subepithelial coats of the mucosa and in phagocytic cells of the lower abdominal lymph nodes and spleen. The density of labelling was greatest in the sperm itself then fell away sharply and uniformly in the other sites. Label was present at this time in sperm spilled into the peritoneal cavity via the needle track. Associated with this spillage, label was seen in peritoneal polymorpho- nuclear granulocytes and macrophages, in macrophages of the uterine wall, and in phagocytic cells of the lymph nodes and spleen. The density of labelling was greatest in the sperm itself but the density decline in these other sites was less than in these same sites resulting from sperm retained wholly in the uterine lumen. Labelled sperm was present in all experiments in the vaginal lumen. The relation between the density of labelling and the degree of degradation of the sperm products is discussed and it is reasoned that the female cells are exposed to less degraded sperm products as a result of entry via the peritoneal cavity than entry via the uterine mucosa. This route may be thereby more effective as an antigenic stimulant.

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STUDIES IN EDEMA

Journal of Experimental Medicine ◽

10.1084/jem.12.4.510 ◽

1910 ◽

Vol 12 (4) ◽

pp. 510-532 ◽

Cited By ~ 3

Author(s):

Moyer S. Fleisher ◽

Leo Loeb

Keyword(s):

Sodium Chloride ◽

Osmotic Pressure ◽

Blood Vessels ◽

Peritoneal Cavity ◽

Peritoneal Fluid ◽

Chloride Content ◽

Body Fluids ◽

The Body ◽

Uranium Nitrate ◽

Rate Of Absorption

I. In normal animals the injection of caffeine slightly diminishes the absorption of fluid from the peritoneal cavity, in spite of the fact that the amount of fluid and sodium chloride eliminated through the kidneys is markedly increased. The lessened absorption of fluid is due to a slight lowering of the osmotic pressure of the blood. II. In nephrectomized animals caffeine increases the absorption of fluid from the peritoneal cavity; the increase in absorption is greater in nephrectomized animals which received caffeine than in nephrectomized animals which did not receive this substance, and it is due to additive increase in the osmotic pressure of the blood. In a similar manner, caffeine increases the absorption of fluid from the peritoneal cavity in animals in which, instead of nephrectomy, other operations, not directly affecting the kidneys, had been performed. In this case also the increase in absorption is presumably preceded by and due to an increase in the osmotic pressure of the blood. III. In animals injected with uranium nitrate three days previously, caffeine diminishes the absorption of fluid from the peritoneal cavity, notwithstanding the high osmotic pressure of the blood which we observe in such animals. This agrees with the results of our previous experiments in which we found that in animals injected with uranium nitrate the absorption of fluid is not increased in spite of the rise of the osmotic pressure of the blood. IV. At the time of the conclusion of the absorption experiments, the amount of fluid retained in the vessels was found to be diminished in each series in which caffeine was used. Only in certain cases can this be due to the increased amount of fluid leaving the blood vessels through the kidneys; in other cases it can only be due to a movement of water from the blood vessels into the tissues caused by the injection of caffeine. V. In normal animals, in nephrectomized animals and in animals in which an operation not directly affecting the kidneys had been performed, caffeine causes an absolute and relative increase in the elimination of sodium chloride from the peritoneal fluid, as a result of which the remaining peritoneal fluid shows a lessened content of sodium chloride. Caffeine causes also a decrease in the sodium chloride content of the blood. We see, therefore, that under the influence of caffeine a greater amount of sodium chloride is eliminated from the body fluids into the tissues or through the kidneys. The factors which cause the sodium chloride to leave the body fluids are probably primarily responsible for the diuresis which takes place after administration of caffeine. In the case of caffeine and other similar substances the diuresis is, therefore, in all probability not due primarily to a specific action of the kidney, but to conditions which affect the distribution of sodium chloride in the body. VI. The distribution coefficient of other osmotically active substances differs from that of sodium chloride. These other substances have a tendency to move into the body fluids in increased quantities under the influence of caffeine. VII. Summarizing all experiments in which we studied the absorption from the peritoneal cavity, we may state that changes in the osmotic pressure of the blood represent the principal factor in explaining the variations in the rate of absorption of fluid from the peritoneal cavity. VIII. There exists no direct relation between an increase in the rate of absorption of fluid from the peritoneal cavity and an increase in the amount of urine secreted. If it should be found that even at a period following the injection of caffeine, later than that at which we have studied the absorption, a rise of the osmotic pressure of the blood does not appear, then we may state that the diminution in the amount of edema in the body cavities resulting from the administration of caffeine is entirely due to an inhibition of the production of edema and not to an increased absorption of fluid from the serous body cavities.

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STUDIES IN EDEMA

Journal of Experimental Medicine ◽

10.1084/jem.12.4.487 ◽

1910 ◽

Vol 12 (4) ◽

pp. 487-509 ◽

Cited By ~ 6

Author(s):

Moyer S. Fleisher ◽

Leo Loeb

Keyword(s):

Sodium Chloride ◽

Osmotic Pressure ◽

Blood Vessels ◽

Peritoneal Cavity ◽

Peritoneal Fluid ◽

Chloride Solution ◽

Sodium Chloride Solution ◽

The Other ◽

Uranium Nitrate ◽

Active Substances

1. In the experiments recorded in this paper the influence of the osmotic pressure of the blood upon absorption of fluid from the peritoneal cavity becomes apparent. Nephrectomy, removal of the adrenals, and other operations increase the osmotic pressure of the blood and increase the absorption of fluid from the peritoneal cavity. On the other hand, ether narcosis, at the period at which we tested its influence, causes neither an increase of osmotic pressure of the blood nor an increase in the absorption of fluid from the peritoneal cavity. 2. The increased osmotic pressure and increased absorption of fluid in nephrectomized animals is to a great extent not a specific effect of the removal of the kidneys, but approximately the same conditions can be observed after incisions of the skin and muscles. 3. After poisoning with uranium nitrate and in cases of peritonitis, complicating factors come into play, and under such conditions the absorption from the peritoneal cavity is not increased, notwithstanding the higher osmotic pressure of the blood. 4. In conditions in which the osmotic pressure of the blood is very high before the injection of sodium chloride solution into the peritoneal cavity (nephrectomized rabbits or rabbits injected with uranium nitrate three days previously), adrenalin causes no increase, or only a very slight one, in the absorption of peritoneal fluid. On the other hand, one day after the injection of uranium nitrate the osmotic pressure of the blood is only slightly increased before the injection of the sodium chloride solution into the peritoneal cavity, and here adrenalin causes a marked increase in absorption of fluid from the peritoneal cavity. 5. In animals injected with uranium nitrate the retention of sodium chloride and other osmotically active substances in the blood is not entirely due to interference with the functions of the kidney. This retention may be explained either by an inability of the tissues to bind the sodium chloride and other osmotically active substances or to a diminished permeability of the blood vessels for such substances. 6. While in nephrectornized animals the elimination of sodium chloride from the peritoneal cavity and also from the blood is increased, in animals injected with uranium nitrate such an elimination is diminished. This increase in the sodium chloride content of the peritoneal fluid in animals treated with uranium nitrate is accompanied by a decrease in the diffusion of other osmotically active substances into the peritoneal cavity. 7. While in nephrectomized animals and in animals injected with uranium nitrate one day previously, adrenalin causes a diminution of the fluid retained in the blood-vessels similar to the diminution noted in normal animals, adrenalin no longer exerts such an effect at a later stage of the uranium nitrate poisoning. At this period after the administration of uranium nitrate, the retention of fluid in the blood vessels is apparently equal in experiments with and without the injection of adrenalin, and following the absorption of fluid from the peritoneal cavity, the retention of fluid in the blood vessels in the uranium nitrate animals is increased comparatively to a greater extent than in normal animals. 8. Our experiments show a marked difference in the distribution of fluid and of osmotically active substances in nephrectomized animals and in animals injected with uranium nitrate. This difference may explain the much greater liability to the development of edema in animals injected with uranium nitrate.

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Ovarian Origin of Plasma and Peritoneal Fluid Prorenin in Early Pregnancy and in Patients with Ovarian Hyperstimulation Syndrome*

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.82.2.3767 ◽

1997 ◽

Vol 82 (2) ◽

pp. 461-464

Author(s):

Joseph Itskovitz-Eldor ◽

Shahar Kol ◽

Nathan Lewit ◽

Jean E. Sealey

Keyword(s):

Ascitic Fluid ◽

Peritoneal Cavity ◽

Early Pregnancy ◽

Plasma Levels ◽

Peritoneal Fluid ◽

Ovarian Hyperstimulation Syndrome ◽

Vascular System ◽

Ovarian Hyperstimulation ◽

Arterial Blood ◽

Wide Range

Abstract Prorenin is the major product of renin gene expression in the ovary. Plasma levels of prorenin are elevated in ovarian-stimulated patients and during early pregnancy. To further elucidate the source of the elevated plasma levels of prorenin, we measured prorenin, renin activity, angiotensinogen, and steroid hormone levels in the plasma, luteal fluids (luteal cysts), ascitic fluid, and in ovarian venous samples collected from a patient with severe ovarian hyperstimulation syndrome (OHSS) and ectopic pregnancy. Prorenin/renin was also measured in plasma and in peritoneal fluid obtained during therapeutic paracentesis from four patients with OHSS. Several corpora luteal fluids were obtained that were rich in estradiol (E2) and progesterone (P). Ovarian venous E2 and P were 20-fold higher than in arterial blood and as high or higher than the levels detected in the luteal fluids. The ratios of the hormonal levels in ascitic fluid and plasma were 1.9 for P and 1.4 for E2. A wide range of prorenin concentrations [1279 ± 918 sd ng/mL/hr, n = 6] were found in corpora luteal fluids, but in each the prorenin concentration was higher than in plasma (494 ng/mL/hr). Prorenin but not renin was higher (+23%) in ovarian venous than arterial blood. Prorenin in the 7 liters of ascitic fluid aspirated (2686 ng/mL/hr) was 5-fold higher than in plasma and similar to the levels measured in the corpora lutea with the highest prorenin concentrations. Renin in luteal cysts and ascitic fluid constituted 3% and 6% of the total renin (renin + prorenin), respectively. Total renin was also higher in peritoneal fluid (1538 ± 925 ng/mL/hr) than in plasma (375 ± 237 ng/mL/hr) of the 4 additional patients with severe OHSS. These findings indicate that the ovary secretes prorenin during early pregnancy and that its secretion is directed preferentially from the luteal cysts into the peritoneal cavity. In light of recent evidence of an effect of prorenin on the vascular system, the presence of a huge reservoir of prorenin in the peritoneal cavity of patients with OHSS suggests a potential role for prorenin in the pathogenesis of this syndrome.

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