scholarly journals Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions

2021 ◽  
Author(s):  
Shinako Kakuda ◽  
Bingchen Li ◽  
Erwin London
Keyword(s):  
Lipid Vesicles ◽  
Lipid Interactions ◽  
Perfringolysin O

scholarly journals Scratching the surface: native mass spectrometry of peripheral membrane protein complexes

2020 ◽  
Vol 48 (2) ◽  
pp. 547-558 ◽  
Author(s):  
Cagla Sahin ◽  
Deseree J. Reid ◽  
Michael T. Marty ◽  
Michael Landreh
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Lipid Vesicles ◽  
Native Mass Spectrometry ◽  
Integral Membrane Proteins ◽  
Structural Basis ◽  
Lipid Interactions ◽  
Native Ms ◽  
Peripheral Membrane Protein

A growing number of integral membrane proteins have been shown to tune their activity by selectively interacting with specific lipids. The ability to regulate biological functions via lipid interactions extends to the diverse group of proteins that associate only peripherally with the lipid bilayer. However, the structural basis of these interactions remains challenging to study due to their transient and promiscuous nature. Recently, native mass spectrometry has come into focus as a new tool to investigate lipid interactions in membrane proteins. Here, we outline how the native MS strategies developed for integral membrane proteins can be applied to generate insights into the structure and function of peripheral membrane proteins. Specifically, native MS studies of proteins in complex with detergent-solubilized lipids, bound to lipid nanodiscs, and released from native-like lipid vesicles all shed new light on the role of lipid interactions. The unique ability of native MS to capture and interrogate protein–protein, protein–ligand, and protein–lipid interactions opens exciting new avenues for the study of peripheral membrane protein biology.

scholarly journals Understanding Sequence Contributions to Peptoid–lipid Interactions: Using Peptoids as a Platform to Advance Multidisciplinary Research and Undergraduate Education in Parallel

2018 ◽  
Author(s):  
Christian Jimenez ◽  
Jiacheng Tan ◽  
Kalli M. Dowell ◽  
Gillian Gadbois ◽  
Cameron Read ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Undergraduate Education ◽  
Lipid Vesicles ◽  
Multidisciplinary Research ◽  
Biophysical Properties ◽  
Educational Value ◽  
Lipid Interactions ◽  
Sequence Features ◽  
Spectroscopy Studies ◽  
Insight Into

<p>The synthesis and fluorescence spectroscopy studies of 21 peptoids,</p> <p>including 19 new tripeptoids, are described. Insight into sequence features that influence biophysical properties in the presence and absence of unilamellar lipid vesicles is provided. Concomitantly, we highlight the educational value of training undergraduates in multidisciplinary research using peptoid science.</p>

Microfluidic Diffusion Platform for Characterizing the Sizes of Lipid Vesicles and the Thermodynamics of Protein–Lipid Interactions

2018 ◽  
Vol 90 (5) ◽  
pp. 3284-3290 ◽  
Author(s):  
Hongze Gang ◽  
Céline Galvagnion ◽  
Georg Meisl ◽  
Thomas Müller ◽  
Manuela Pfammatter ◽  
...  
Keyword(s):  
Lipid Vesicles ◽  
Lipid Interactions

Conjugated polyelectrolyte–lipid interactions: Opportunities in biosensing

2010 ◽  
Vol 83 (1) ◽  
pp. 43-55 ◽  
Author(s):  
An Thien Ngo ◽  
Pierre Karam ◽  
Gonzalo Cosa
Keyword(s):  
Conjugated Polymers ◽  
Energy Transport ◽  
Lipid Vesicles ◽  
Electronic Energy ◽  
Polymer Backbone ◽  
Conjugated Polyelectrolyte ◽  
Lipid Interactions ◽  
Efficient Energy ◽  
The Past ◽  
Considerable Research

Fluorescent conjugated polyelectrolytes (CPEs) have attracted considerable interest over the past decade as novel materials for developing biosensing schemes and sensing devices for biomolecules. This interest stems from the exquisite polymer sensitivity to the presence of fluorescence quenchers, enabling amplified sensing of molecules of interest. Efficient energy transport along the polymer backbone is critical to their sensing capabilities. Considerable research efforts have thus gone into understanding and controlling energy transport along the polymer backbone. In particular, it has been shown that interactions between CPEs with either surfactants or lipid molecules may significantly reduce energy transport along the polymer backbone that in turn may provide for unique biosensing opportunities. In the first half of this review, we give a historical overview on energy transport in conjugated polymers and polyelectrolytes. In the second half, we summarize the most recent work on the interaction of CPEs with surfactants with an emphasis on our own work elucidating electronic energy transport in CPEs encapsulated into lipid vesicles or embedded within the membrane of lipid vesicles.

scholarly journals Imaging of intermittent lipid-receptor interactions reflects changes in live cell membranes upon agonist-receptor binding

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Johan Tornmalm ◽  
Joachim Piguet ◽  
Volodymyr Chmyrov ◽  
Jerker Widengren
Keyword(s):  
Triplet State ◽  
Cell Membranes ◽  
Plasma Membranes ◽  
Lipid Vesicles ◽  
Live Cell ◽  
Spin Labels ◽  
State Transitions ◽  
Collisional Quenching ◽  
Lipid Interactions ◽  
Gpcr Activation

AbstractProtein-lipid interactions in cellular membranes modulate central cellular functions, are often transient in character, but occur too intermittently to be readily observable. We introduce transient state imaging (TRAST), combining sensitive fluorescence detection of fluorophore markers with monitoring of their dark triplet state transitions, allowing imaging of such protein-lipid interactions. We first determined the dark state kinetics of the biomembrane fluorophore 7-nitrobenz-2-oxa-1,3-diazole-4-yl (NBD) in lipid vesicles, and how its triplet state is quenched by spin-labels in the same membranes. We then monitored collisional quenching of NBD-lipid derivatives by spin-labelled stearic acids in live cell plasma membranes, and of NBD-lipid derivatives by spin-labelled G-Protein Coupled Receptors (GPCRs). We could then resolve transient interactions between the GPCRs and different lipids, how these interactions changed upon GPCR activation, thereby demonstrating a widely applicable means to image and characterize transient molecular interactions in live cell membranes in general, not within reach via traditional fluorescence readouts.

scholarly journals Influence of Lipid Composition on Pediocin PA-1 Binding to Phospholipid Vesicles

1998 ◽  
Vol 64 (9) ◽  
pp. 3530-3532 ◽  
Author(s):  
Yuhuan Chen ◽  
Richard D. Ludescher ◽  
Thomas J. Montville
Keyword(s):  
Fatty Acid ◽  
Electrostatic Interaction ◽  
Lipid Content ◽  
Interaction Model ◽  
Lipid Vesicles ◽  
Phospholipid Vesicles ◽  
Binding Parameters ◽  
Lipid Concentration ◽  
Anionic Lipid ◽  
Lipid Interactions

ABSTRACT Pediocin PA-1 bound to anionic lipid vesicles with saturated or unsaturated fatty acid chains in a lipid concentration-dependent fashion. Little change in binding parameters was observed for zwitterionic lipid vesicles. Decreasing the anionic lipid content of the vesicles gave a higher relative dissociation constant for the peptide-lipid interactions and further supports the electrostatic interaction model of binding.

scholarly journals Understanding Sequence Contributions to Peptoid–lipid Interactions: Using Peptoids as a Platform to Advance Multidisciplinary Research and Undergraduate Education in Parallel

2018 ◽  
Author(s):  
Christian Jimenez ◽  
Jiacheng Tan ◽  
Kalli M. Dowell ◽  
Gillian Gadbois ◽  
Cameron Read ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Undergraduate Education ◽  
Lipid Vesicles ◽  
Multidisciplinary Research ◽  
Biophysical Properties ◽  
Educational Value ◽  
Lipid Interactions ◽  
Sequence Features ◽  
Spectroscopy Studies ◽  
Insight Into

<p>The synthesis and fluorescence spectroscopy studies of 21 peptoids,</p> <p>including 19 new tripeptoids, are described. Insight into sequence features that influence biophysical properties in the presence and absence of unilamellar lipid vesicles is provided. Concomitantly, we highlight the educational value of training undergraduates in multidisciplinary research using peptoid science.</p>

The structure of membranes and membrane proteins embedded in amorphous ice

1992 ◽  
Vol 50 (1) ◽  
pp. 432-433
Author(s):  
Uwe Lücken ◽  
Joachim Jäger
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Membrane Proteins ◽  
Phase Transition Temperature ◽  
Lipid Vesicles ◽  
Freezing Stress ◽  
Amorphous Ice ◽  
Different Temperatures ◽  
Band Pass ◽  
Lipid Polymorphism

TEM imaging of frozen-hydrated lipid vesicles has been done by several groups Thermotrophic and lyotrophic polymorphism has been reported. By using image processing, computer simulation and tilt experiments, we tried to learn about the influence of freezing-stress and defocus artifacts on the lipid polymorphism and fine structure of the bilayer profile. We show integrated membrane proteins do modulate the bilayer structure and the morphology of the vesicles.Phase transitions of DMPC vesicles were visualized after freezing under equilibrium conditions at different temperatures in a controlled-environment vitrification system. Below the main phase transition temperature of 24°C (Fig. 1), vesicles show a facetted appearance due to the quasicrystalline areas. A gradual increase in temperature leads to melting processes with different morphology in the bilayer profile. Far above the phase transition temperature the bilayer profile is still present. In the band-pass-filtered images (Fig. 2) no significant change in the width of the bilayer profile is visible.

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