Studies of lipid turnover in cells with stable isotope and gas chromatograph–mass spectrometry

2004 ◽  
Vol 325 (2) ◽  
pp. 344-353 ◽  
Author(s):  
Kou-Yi Tserng ◽  
Ronda Griffin
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Gas Chromatograph ◽  
Lipid Turnover ◽  
Gas Chromatograph Mass Spectrometry ◽  
In Cells

scholarly journals Potential insecticidal activity of four essential oils against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7767-7783
Author(s):  
Mohamed E. Tawfeek ◽  
Hayssam M. Ali ◽  
Mohammad Akrami ◽  
Mohamed Z. M. Salem
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Sitophilus Oryzae ◽  
Imperata Cylindrica ◽  
Rice Weevil ◽  
Gas Chromatograph ◽  
Cymbopogon Citratus ◽  
Phytochemical Composition ◽  
Germacrene D ◽  
Gas Chromatograph Mass Spectrometry

Oils extracted from Cymbopogon citratus, Lantana camara, Artemisia camphorata, and Imperata cylindrica plants were used as potential insecticides against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae). The phytochemical composition of the isolated oils was identified by gas chromatograph-mass spectrometry (GC-MS). Oil contact toxicities were evaluated against the adults of S. oryzae. The activities of acetylcholinesterase (AChE), alkaline phosphatase (ALP), and transaminases enzymes (AST) were measured. L. camara oil (LC50 = 9.81 mg/cm2) demonstrated the highest effect, followed by C. citratus oil (LC50 = 10.89 mg/cm2), A. camphorata EO (LC50 = 16.12 mg/cm2), and I. cylindrica oil (LC50= 36.85 mg/cm2) against the adults of S. oryzae. The inhibition percentages of AChE were 38.8, 41.7, 35.0, and 27.2%; ALP were 42.4, 49.3, 28.1, and 18.7%; AST were 33.9, 38.7, 20.8, and 11.8%; and ALT were 22.7, 30.5, 14.6, and 9.6% after treated S. oryzae with oils from C. citratus, L. camara, A. camphorata and I. cylindrica, respectively. The highest abundant compounds in C. citratus were geranial (25.95%), nerylacetal (8.85%), and neral (8.45%), in L. camara were caryophyllene (12.2%), and 3-elemene (8.89%), in A. camphorata were germacrene D-4-ol (20.83%), and borneol (19.47%), and in I. cylindrica were 5-phenylundecane (10.68%), and 6-phenyldodecane (8.70%).

scholarly journals Decomposition Characteristics of Benzene, Toluene and Xylene in an Atmospheric Pressure DC Corona Discharge II. Characteristics of Deposited By-products and Decomposition Process

2016 ◽  
Vol 19 (1) ◽  
Author(s):  
Seiji Sakai ◽  
Kazuhiro Takahashi ◽  
Kohki Satoh ◽  
Hidenori Itoh
Keyword(s):  
Mass Spectrometry ◽  
Carbon Dioxide ◽  
Corona Discharge ◽  
Atmospheric Pressure ◽  
Infrared Absorption Spectroscopy ◽  
Gas Chromatograph ◽  
Decomposition Processes ◽  
Benzene Toluene ◽  
Gas Chromatograph Mass Spectrometry ◽  
By Products

AbstractGaseous by-products and deposited material obtained from the decomposition of benzene, toluene and xylene in an atmospheric pressure DC corona discharge were minutely investigated by gas chromatograph mass spectrometry and infrared absorption spectroscopy, and the decomposition processes of benzene, toluene and xylene were estimated. It was found that carbon dioxide (CO

scholarly journals Integrated Gas Chromatograph-Mass Spectrometry (GC/MS) and MS/MS-Based Molecular Networking Reveals the Analgesic and Anti-Inflammatory Phenotypes of the Sea Slater Ligia exotica

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 395 ◽  
Author(s):  
Yang Yue ◽  
Quanbin Zhang ◽  
Jing Wang
Keyword(s):  
Mass Spectrometry ◽  
Folk Medicine ◽  
Chemical Diversity ◽  
Biologically Active ◽  
Gas Chromatograph ◽  
Molecular Networking ◽  
In Vivo Models ◽  
Analgesic Effects ◽  
Anti Inflammatory ◽  
Gas Chromatograph Mass Spectrometry

The sea slater Ligia exotica is believed to have effects of reducing swelling and relieving pain in Chinese folk medicine. However, the scientific foundation of using the sea slater Ligia spp. as an analgesic and anti-inflammatory material remains elusive. In the present study, various organic extracts from sea slater L. exotica were subjected to biological screening employing in vitro and in vivo models, and chemical phenotypes of the biologically active extract were deciphered by integrated gas chromatograph-mass spectrometry (GC-MS) profiling and MS/MS-based molecular networking. The results demonstrated, for the first time, that petroleum ether extract (PE) from L. exotica possessed remarkable anti-inflammatory and analgesic effects. Moreover, intragastric administration of PE at 200 mg/kg produced analgesic effects in both the writhing test and hot plate test. GC-MS analysis revealed that Z-9-hexadecenoic acid and 6-octadecenoic acid dominated in the volatile compositions of PE. Molecular networking (MN) suggested great chemical diversity within L. exotica. In total, 69 known compounds were identified in Ligia extracts by MS/MS spectral matching, and at least 7 analogues from two clusters of nitrogen-containing compounds (MN3,4) were strongly suggested as novel compounds. The molecular families MN1,3,4 were almost exclusively detected in the biologically active PE and ethyl acetate extract (EE). Importantly, various known compounds identified in MN1 were reported to possess analgesic and anti-inflammatory effects in the literature, which may contribute to the observed analgesic and anti-inflammatory effects of L. exotica. The present study not only demonstrated the ethnopharmaceutical value of L. exotica for pain-relief in Chinese folk medicine, but also suggested that sea slaters may represent a promising source for discovery of novel analgesic and anti-inflammatory compounds in the near future.

Sign in / Sign up

Export Citation Format

Share Document