Detection and quantification of tau aggregation using a membrane filter assay

Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment.Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23–79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4′,6′-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer.Results. The mean ± SD of CFU in the sample of “#25 First” was(1.0±1.4)×105. As the root canal treatment progressed, the CFU decreased as7.9×103(#55 First) and4.3×102(#55 Second).Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

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[24] Membrane filter assay for detection of amyloid-like polyglutamine-containing protein aggregates

Methods in Enzymology - Amyloid, Prions, and Other Protein Aggregates ◽

10.1016/s0076-6879(99)09026-6 ◽

1999 ◽

pp. 375-386 ◽

Cited By ~ 165

Author(s):

Erich E. Wanker ◽

Eberhard Scherzinger ◽

Volker Heiser ◽

Annie Sittler ◽

Holger Eickhoff ◽

...

Keyword(s):

Membrane Filter ◽

Protein Aggregates ◽

Filter Assay

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Detection and Quantification Methods for Fibrillar Products of In Vitro Tau Aggregation Assays

Methods in Molecular Biology - Tau Protein ◽

10.1007/978-1-4939-6598-4_6 ◽

2016 ◽

pp. 101-111 ◽

Cited By ~ 5

Author(s):

Niki Nanavaty ◽

Lauren Lin ◽

Samantha H. Hinckley ◽

Jeff Kuret

Keyword(s):

Tau Aggregation ◽

Detection And Quantification

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A membrane filter assay for protein sulfhydryl groups

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(71)90622-x ◽

1971 ◽

Vol 44 (2) ◽

pp. 453-458 ◽

Cited By ~ 17

Author(s):

Joseph S. Krakow ◽

Sara P. Goolsby

Keyword(s):

Membrane Filter ◽

Sulfhydryl Groups ◽

Protein Sulfhydryl ◽

Filter Assay ◽

Protein Sulfhydryl Groups

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Evaluation of a Membrane Filter Assay System, Ortho HCV Ab Quik Pack, for Detection of Anti-Hepatitis C Virus Antibody

Journal of Clinical Microbiology ◽

10.1128/jcm.36.5.1439-1440.1998 ◽

1998 ◽

Vol 36 (5) ◽

pp. 1439-1440 ◽

Cited By ~ 2

Author(s):

Takanari Kodama ◽

Satoshi Ichiyama ◽

Kumiko Sato ◽

Toshi Nada ◽

Nobuo Nakashima

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Sensitivity And Specificity ◽

Membrane Filter ◽

Assay System ◽

Virus Antibody ◽

Serum Samples ◽

Filter Assay ◽

Immunosorbent Assays ◽

Hepatitis C Virus Antibody

A simple membrane immunoassay assay system, Quik Pack, for the detection of hepatitis C virus antibody was compared with two enzyme-linked immunosorbent assays (ELISAs) in a study of 600 serum samples. Quik Pack exhibited excellent sensitivity and specificity: 96.0 and 99.7%, respectively, versus the ELISA-2 and 99.7 and 99.4%, respectively, versus the ELISA-3.

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MEMBRANE FILTER ASSAY FOR DETECTION OF ENTEROTOXIGENIC ESCHERICHIA COLI IN EPIDEMIOLOGICAL STUDIES

The Lancet ◽

10.1016/s0140-6736(86)91274-2 ◽

1986 ◽

Vol 327 (8488) ◽

pp. 1007-1009 ◽

Cited By ~ 1

Author(s):

Jamuna Vadivelu ◽

BohumilS. Drasar ◽

NigelP. Cox ◽

BarryJ. Lloyd ◽

RichardG. Feachem ◽

...

Keyword(s):

Escherichia Coli ◽

Membrane Filter ◽

Epidemiological Studies ◽

Enterotoxigenic Escherichia Coli ◽

Filter Assay

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The utility of the AC/TC ratio for watershed management: a case study

Water Science & Technology ◽

10.2166/wst.2004.0054 ◽

2004 ◽

Vol 50 (1) ◽

pp. 199-203 ◽

Cited By ~ 7

Author(s):

J. Booth ◽

G.M. Brion

Keyword(s):

Watershed Management ◽

Membrane Filter ◽

Total Coliform ◽

Faecal Coliforms ◽

Faecal Material ◽

Total Coliforms ◽

Filter Assay ◽

The Impact ◽

Faecal Streptococci

A human-impacted watershed was monitored during the dry summer seasons in 2002 and 2003 to investigate the impact of providing access to sewer mains to local village residences. Faecal coliform concentrations were monitored at select sites along the 30-mile stretch of creek, together with faecal streptococci, enterococci and total coliforms. Analysis of the results found that levels of faecal coliforms were inadequate at identifying significant known influxes of human and animal sewage established by sanitary survey. However, the bacterial ratio of atypical colonies to total coliform colonies (AC/TC), obtained from the total coliform membrane filter assay on m-Endo media, correctly indexed human faecal impact of inadequately sewered villages located along the creek. In addition, the AC/TC ratio correctly classified the predominant source of faecal runoff in the creek headwaters as agricultural, and indicated when aged agricultural faecal material was introduced by tributaries. An approach for watershed management that uses the AC/TC ratio in addition to levels of bacteria is proposed.

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EnterotoxigenicEscherichia coliin the domestic environment of a Malaysian village

Epidemiology and Infection ◽

10.1017/s0950268800030909 ◽

1989 ◽

Vol 103 (3) ◽

pp. 497-511 ◽

Cited By ~ 5

Author(s):

J. Vadivelu ◽

R. G. Feachem ◽

B. S. Drasar ◽

T. J. Harrison ◽

N. Parasakthi ◽

...

Keyword(s):

Dna Hybridization ◽

Membrane Filter ◽

Food Samples ◽

Food Preparation ◽

Hybridization Assay ◽

Faecal Coliforms ◽

Weaning Food ◽

Stool Samples ◽

Filter Assay ◽

Children Under 5

SUMMARYThe membrane-filter assay, GM1-ELISA, and DNA-DNA hybridization assay, were used to detect enterotoxigenicEscherichia coli(ETEC) in samples of water, weaning food, food preparation surface swabs, fingerprints of mothers, and the fingerprints and stools of children under 5 years of age, in 20 households in a Malaysian village. Weaning food and environmental samples were frequently contaminated by faecal coliforms, including ETEC. The membrane-filter assay detected and enumerated faecal coliforms and LT-ETEC in all types of water and weaning food samples. Highest concentrations of faecal coliforms and LT-ETEC were found in weaning food, followed by well-water, stored water and stored drinking water. The GM1-ELISA detected LT-ETEC in weaning food, food preparation surfaces, fingerprints and stool samples. The DNA-DNA hybridization assay detected a larger proportion of STa2-ETEC than the other toxotypes, either singly or in combination. All the assays in combination detected the presence of ETEC in all types of samples on at least one occasion in each household. It was not possible to classify households as consistently more or less contaminated with ETEC. On individual occasions it was possible to show a significant association of the presence of LT-ETEC between the fingerprints of children and their stools, fingerprints of mothers and children, and weaning food and the stools of the child consuming the food.

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